Description of Sporanaerobium hydrogeniformans gen. nov., sp. nov., an obligately anaerobic, hydrogen-producing bacterium isolated from Aravali hot spring in India
An obligately anaerobic bacterium XHS1971 T , capable of degrading cellulose and xylan, was isolated from a sediment sample of Aravali hot spring, Ratnagiri, India. Cells of strain XHS1971 T were Gram-stain-negative, spore-forming, motile, long-rods. Growth was observed at temperatures 30–50 °C (opt...
Gespeichert in:
| Veröffentlicht in: | Archives of microbiology 2023-09, Vol.205 (9), p.305-305, Article 305 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 305 |
|---|---|
| container_issue | 9 |
| container_start_page | 305 |
| container_title | Archives of microbiology |
| container_volume | 205 |
| creator | Hivarkar, Sai Suresh Vasudevan, Gowdaman Dhakephalkar, Prashant K. Dagar, Sumit Singh |
| description | An obligately anaerobic bacterium XHS1971
T
, capable of degrading cellulose and xylan, was isolated from a sediment sample of Aravali hot spring, Ratnagiri, India. Cells of strain XHS1971
T
were Gram-stain-negative, spore-forming, motile, long-rods. Growth was observed at temperatures 30–50 °C (optimum 40–45 °C), pH 5.0–10.0 (optimum pH 8.0) and NaCl concentrations 0–0.5% (optimum 0%). Generation time of strain XHS1971
T
was 5 h under optimised growth conditions. Strain XHS1971
T
showed the ability to metabolise different complex and simple sugars constituting lignocellulosic biomass. Glucose was fermented majorly into hydrogen, formic acid, acetic acid, and ethanol, whereas carbon dioxide, butyric acid, lactic acid and succinic acid were produced in traces. 16S rRNA gene analysis of strain XHS1971
T
revealed 5%) were C
14:0
, C
16:0
, C
18:0
, and C
16:1
ω7
c
. The genome size of the strain was 3.74 Mb with 35.3 mol% G + C content, and genes were annotated to carbohydrate metabolism, including genes involved in the degradation of cellulose and xylan and the production of hydrogen, ethanol and acetate. The uniqueness of strain was further validated by digital DNA-DNA hybridisation (dDDH), Average Nucleotide Identity (ANI), and Average Amino Acid Identity (AAI) values of 22%, 80%, and 63%, respectively, with nearest phylogenetic affiliates. Based on the detailed analyses, we propose a new genus and species,
Sporanaerobium hydrogeniformans
gen. nov., sp. nov., for strain XHS1971
T
(= MCC3498
T
= KCTC15729
T
= JCM32657
T
) within family
Lachnospiraceae
. |
| doi_str_mv | 10.1007/s00203-023-03641-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3040475545</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2849391409</sourcerecordid><originalsourceid>FETCH-LOGICAL-c452t-7efca0d995968feecacd4d2a522398bf9ee0e117911b3fcc754bed16ad9f615c3</originalsourceid><addsrcrecordid>eNqFkc1u1DAUhS0EokPhBVggS2xYNIN_k3hZlRYqVeqiILGLHPt66iqxBzupNM_Di-IhnSJ1URaW7-I7x9fnIPSekjUlpPmcCWGEV4SVw2tBq_oFWlHBWUUa9vMlWhFOWNUqzo_Qm5zvCKGsbdvX6Ig3smG0rlfo9xfIJvnt5GPA0eGbbUw6aEix9_OIb3c2xQ0E72Iadci4zGsc4v36BOftYdJF2g9-oycYdvggNyeP8mqbop2NDxvcazNB2nv7HIeisNilOOLTpO_14PFtnIpz2qM-4MtgvX6LXjk9ZHj3cB-jHxfn38--VVfXXy_PTq8qIySbqgac0cQqJVXdOgCjjRWWackYV23vFAABShtFac-dMY0UPVhaa6tcTaXhx-jT4lu2_TVDnrrRZwPDoAPEOXecCCIaKYX8L8paWcIXUrQF_fgEvYtzCuUjhRKKKyqIKhRbKJNizglcVzIYddp1lHT7trul7a603f1tu6uL6MOD9dyPYB8lh3oLwBdgSRTSv7efsf0DIO63uQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2849391409</pqid></control><display><type>article</type><title>Description of Sporanaerobium hydrogeniformans gen. nov., sp. nov., an obligately anaerobic, hydrogen-producing bacterium isolated from Aravali hot spring in India</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Hivarkar, Sai Suresh ; Vasudevan, Gowdaman ; Dhakephalkar, Prashant K. ; Dagar, Sumit Singh</creator><creatorcontrib>Hivarkar, Sai Suresh ; Vasudevan, Gowdaman ; Dhakephalkar, Prashant K. ; Dagar, Sumit Singh</creatorcontrib><description>An obligately anaerobic bacterium XHS1971
T
, capable of degrading cellulose and xylan, was isolated from a sediment sample of Aravali hot spring, Ratnagiri, India. Cells of strain XHS1971
T
were Gram-stain-negative, spore-forming, motile, long-rods. Growth was observed at temperatures 30–50 °C (optimum 40–45 °C), pH 5.0–10.0 (optimum pH 8.0) and NaCl concentrations 0–0.5% (optimum 0%). Generation time of strain XHS1971
T
was 5 h under optimised growth conditions. Strain XHS1971
T
showed the ability to metabolise different complex and simple sugars constituting lignocellulosic biomass. Glucose was fermented majorly into hydrogen, formic acid, acetic acid, and ethanol, whereas carbon dioxide, butyric acid, lactic acid and succinic acid were produced in traces. 16S rRNA gene analysis of strain XHS1971
T
revealed < 94.5% homology with
Cellulosilyticum lentocellum
DSM5427
T
followed by
Cellulosilyticum ruminicola
JCM14822
T
, identifying strain as a distinct member of family
Lachnospiraceae
. The major cellular fatty acids (> 5%) were C
14:0
, C
16:0
, C
18:0
, and C
16:1
ω7
c
. The genome size of the strain was 3.74 Mb with 35.3 mol% G + C content, and genes were annotated to carbohydrate metabolism, including genes involved in the degradation of cellulose and xylan and the production of hydrogen, ethanol and acetate. The uniqueness of strain was further validated by digital DNA-DNA hybridisation (dDDH), Average Nucleotide Identity (ANI), and Average Amino Acid Identity (AAI) values of 22%, 80%, and 63%, respectively, with nearest phylogenetic affiliates. Based on the detailed analyses, we propose a new genus and species,
Sporanaerobium hydrogeniformans
gen. nov., sp. nov., for strain XHS1971
T
(= MCC3498
T
= KCTC15729
T
= JCM32657
T
) within family
Lachnospiraceae
.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-023-03641-6</identifier><identifier>PMID: 37572166</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>acetates ; Acetic acid ; Amino acids ; Anaerobiosis ; Bacteria ; Bacteria, Anaerobic - genetics ; Bacterial Typing Techniques ; Base Composition ; Biochemistry ; biomass ; Biomedical and Life Sciences ; Biotechnology ; Butyric acid ; Carbohydrate metabolism ; Carbohydrates ; Carbon dioxide ; Cell Biology ; Cellulose ; Cellulose - metabolism ; Deoxyribonucleic acid ; DNA ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; Ecology ; Ethanol ; family ; Fatty acids ; Fatty Acids - analysis ; Formic acid ; Genes ; Genomes ; glucose ; Growth conditions ; Homology ; Hot springs ; Hot Springs - microbiology ; Hybridization ; Hydrogen ; Hydrogen - metabolism ; Hydrogen production ; India ; Lachnospiraceae ; Lactic acid ; Life Sciences ; Lignocellulose ; Microbial Ecology ; Microbiology ; New genera ; new genus ; New species ; Nucleotides ; Original Paper ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Sediment samplers ; sediments ; Sequence Analysis, DNA ; Sodium chloride ; species ; Strain analysis ; Succinic acid ; Xylan ; Xylans</subject><ispartof>Archives of microbiology, 2023-09, Vol.205 (9), p.305-305, Article 305</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-7efca0d995968feecacd4d2a522398bf9ee0e117911b3fcc754bed16ad9f615c3</citedby><cites>FETCH-LOGICAL-c452t-7efca0d995968feecacd4d2a522398bf9ee0e117911b3fcc754bed16ad9f615c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-023-03641-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-023-03641-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37572166$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hivarkar, Sai Suresh</creatorcontrib><creatorcontrib>Vasudevan, Gowdaman</creatorcontrib><creatorcontrib>Dhakephalkar, Prashant K.</creatorcontrib><creatorcontrib>Dagar, Sumit Singh</creatorcontrib><title>Description of Sporanaerobium hydrogeniformans gen. nov., sp. nov., an obligately anaerobic, hydrogen-producing bacterium isolated from Aravali hot spring in India</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>An obligately anaerobic bacterium XHS1971
T
, capable of degrading cellulose and xylan, was isolated from a sediment sample of Aravali hot spring, Ratnagiri, India. Cells of strain XHS1971
T
were Gram-stain-negative, spore-forming, motile, long-rods. Growth was observed at temperatures 30–50 °C (optimum 40–45 °C), pH 5.0–10.0 (optimum pH 8.0) and NaCl concentrations 0–0.5% (optimum 0%). Generation time of strain XHS1971
T
was 5 h under optimised growth conditions. Strain XHS1971
T
showed the ability to metabolise different complex and simple sugars constituting lignocellulosic biomass. Glucose was fermented majorly into hydrogen, formic acid, acetic acid, and ethanol, whereas carbon dioxide, butyric acid, lactic acid and succinic acid were produced in traces. 16S rRNA gene analysis of strain XHS1971
T
revealed < 94.5% homology with
Cellulosilyticum lentocellum
DSM5427
T
followed by
Cellulosilyticum ruminicola
JCM14822
T
, identifying strain as a distinct member of family
Lachnospiraceae
. The major cellular fatty acids (> 5%) were C
14:0
, C
16:0
, C
18:0
, and C
16:1
ω7
c
. The genome size of the strain was 3.74 Mb with 35.3 mol% G + C content, and genes were annotated to carbohydrate metabolism, including genes involved in the degradation of cellulose and xylan and the production of hydrogen, ethanol and acetate. The uniqueness of strain was further validated by digital DNA-DNA hybridisation (dDDH), Average Nucleotide Identity (ANI), and Average Amino Acid Identity (AAI) values of 22%, 80%, and 63%, respectively, with nearest phylogenetic affiliates. Based on the detailed analyses, we propose a new genus and species,
Sporanaerobium hydrogeniformans
gen. nov., sp. nov., for strain XHS1971
T
(= MCC3498
T
= KCTC15729
T
= JCM32657
T
) within family
Lachnospiraceae
.</description><subject>acetates</subject><subject>Acetic acid</subject><subject>Amino acids</subject><subject>Anaerobiosis</subject><subject>Bacteria</subject><subject>Bacteria, Anaerobic - genetics</subject><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>Biochemistry</subject><subject>biomass</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Butyric acid</subject><subject>Carbohydrate metabolism</subject><subject>Carbohydrates</subject><subject>Carbon dioxide</subject><subject>Cell Biology</subject><subject>Cellulose</subject><subject>Cellulose - metabolism</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>Ecology</subject><subject>Ethanol</subject><subject>family</subject><subject>Fatty acids</subject><subject>Fatty Acids - analysis</subject><subject>Formic acid</subject><subject>Genes</subject><subject>Genomes</subject><subject>glucose</subject><subject>Growth conditions</subject><subject>Homology</subject><subject>Hot springs</subject><subject>Hot Springs - microbiology</subject><subject>Hybridization</subject><subject>Hydrogen</subject><subject>Hydrogen - metabolism</subject><subject>Hydrogen production</subject><subject>India</subject><subject>Lachnospiraceae</subject><subject>Lactic acid</subject><subject>Life Sciences</subject><subject>Lignocellulose</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>New genera</subject><subject>new genus</subject><subject>New species</subject><subject>Nucleotides</subject><subject>Original Paper</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Sediment samplers</subject><subject>sediments</subject><subject>Sequence Analysis, DNA</subject><subject>Sodium chloride</subject><subject>species</subject><subject>Strain analysis</subject><subject>Succinic acid</subject><subject>Xylan</subject><subject>Xylans</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkc1u1DAUhS0EokPhBVggS2xYNIN_k3hZlRYqVeqiILGLHPt66iqxBzupNM_Di-IhnSJ1URaW7-I7x9fnIPSekjUlpPmcCWGEV4SVw2tBq_oFWlHBWUUa9vMlWhFOWNUqzo_Qm5zvCKGsbdvX6Ig3smG0rlfo9xfIJvnt5GPA0eGbbUw6aEix9_OIb3c2xQ0E72Iadci4zGsc4v36BOftYdJF2g9-oycYdvggNyeP8mqbop2NDxvcazNB2nv7HIeisNilOOLTpO_14PFtnIpz2qM-4MtgvX6LXjk9ZHj3cB-jHxfn38--VVfXXy_PTq8qIySbqgac0cQqJVXdOgCjjRWWackYV23vFAABShtFac-dMY0UPVhaa6tcTaXhx-jT4lu2_TVDnrrRZwPDoAPEOXecCCIaKYX8L8paWcIXUrQF_fgEvYtzCuUjhRKKKyqIKhRbKJNizglcVzIYddp1lHT7trul7a603f1tu6uL6MOD9dyPYB8lh3oLwBdgSRTSv7efsf0DIO63uQ</recordid><startdate>20230901</startdate><enddate>20230901</enddate><creator>Hivarkar, Sai Suresh</creator><creator>Vasudevan, Gowdaman</creator><creator>Dhakephalkar, Prashant K.</creator><creator>Dagar, Sumit Singh</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20230901</creationdate><title>Description of Sporanaerobium hydrogeniformans gen. nov., sp. nov., an obligately anaerobic, hydrogen-producing bacterium isolated from Aravali hot spring in India</title><author>Hivarkar, Sai Suresh ; Vasudevan, Gowdaman ; Dhakephalkar, Prashant K. ; Dagar, Sumit Singh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-7efca0d995968feecacd4d2a522398bf9ee0e117911b3fcc754bed16ad9f615c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>acetates</topic><topic>Acetic acid</topic><topic>Amino acids</topic><topic>Anaerobiosis</topic><topic>Bacteria</topic><topic>Bacteria, Anaerobic - genetics</topic><topic>Bacterial Typing Techniques</topic><topic>Base Composition</topic><topic>Biochemistry</topic><topic>biomass</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Butyric acid</topic><topic>Carbohydrate metabolism</topic><topic>Carbohydrates</topic><topic>Carbon dioxide</topic><topic>Cell Biology</topic><topic>Cellulose</topic><topic>Cellulose - metabolism</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>Ecology</topic><topic>Ethanol</topic><topic>family</topic><topic>Fatty acids</topic><topic>Fatty Acids - analysis</topic><topic>Formic acid</topic><topic>Genes</topic><topic>Genomes</topic><topic>glucose</topic><topic>Growth conditions</topic><topic>Homology</topic><topic>Hot springs</topic><topic>Hot Springs - microbiology</topic><topic>Hybridization</topic><topic>Hydrogen</topic><topic>Hydrogen - metabolism</topic><topic>Hydrogen production</topic><topic>India</topic><topic>Lachnospiraceae</topic><topic>Lactic acid</topic><topic>Life Sciences</topic><topic>Lignocellulose</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>New genera</topic><topic>new genus</topic><topic>New species</topic><topic>Nucleotides</topic><topic>Original Paper</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Sediment samplers</topic><topic>sediments</topic><topic>Sequence Analysis, DNA</topic><topic>Sodium chloride</topic><topic>species</topic><topic>Strain analysis</topic><topic>Succinic acid</topic><topic>Xylan</topic><topic>Xylans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hivarkar, Sai Suresh</creatorcontrib><creatorcontrib>Vasudevan, Gowdaman</creatorcontrib><creatorcontrib>Dhakephalkar, Prashant K.</creatorcontrib><creatorcontrib>Dagar, Sumit Singh</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hivarkar, Sai Suresh</au><au>Vasudevan, Gowdaman</au><au>Dhakephalkar, Prashant K.</au><au>Dagar, Sumit Singh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Description of Sporanaerobium hydrogeniformans gen. nov., sp. nov., an obligately anaerobic, hydrogen-producing bacterium isolated from Aravali hot spring in India</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><addtitle>Arch Microbiol</addtitle><date>2023-09-01</date><risdate>2023</risdate><volume>205</volume><issue>9</issue><spage>305</spage><epage>305</epage><pages>305-305</pages><artnum>305</artnum><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>An obligately anaerobic bacterium XHS1971
T
, capable of degrading cellulose and xylan, was isolated from a sediment sample of Aravali hot spring, Ratnagiri, India. Cells of strain XHS1971
T
were Gram-stain-negative, spore-forming, motile, long-rods. Growth was observed at temperatures 30–50 °C (optimum 40–45 °C), pH 5.0–10.0 (optimum pH 8.0) and NaCl concentrations 0–0.5% (optimum 0%). Generation time of strain XHS1971
T
was 5 h under optimised growth conditions. Strain XHS1971
T
showed the ability to metabolise different complex and simple sugars constituting lignocellulosic biomass. Glucose was fermented majorly into hydrogen, formic acid, acetic acid, and ethanol, whereas carbon dioxide, butyric acid, lactic acid and succinic acid were produced in traces. 16S rRNA gene analysis of strain XHS1971
T
revealed < 94.5% homology with
Cellulosilyticum lentocellum
DSM5427
T
followed by
Cellulosilyticum ruminicola
JCM14822
T
, identifying strain as a distinct member of family
Lachnospiraceae
. The major cellular fatty acids (> 5%) were C
14:0
, C
16:0
, C
18:0
, and C
16:1
ω7
c
. The genome size of the strain was 3.74 Mb with 35.3 mol% G + C content, and genes were annotated to carbohydrate metabolism, including genes involved in the degradation of cellulose and xylan and the production of hydrogen, ethanol and acetate. The uniqueness of strain was further validated by digital DNA-DNA hybridisation (dDDH), Average Nucleotide Identity (ANI), and Average Amino Acid Identity (AAI) values of 22%, 80%, and 63%, respectively, with nearest phylogenetic affiliates. Based on the detailed analyses, we propose a new genus and species,
Sporanaerobium hydrogeniformans
gen. nov., sp. nov., for strain XHS1971
T
(= MCC3498
T
= KCTC15729
T
= JCM32657
T
) within family
Lachnospiraceae
.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>37572166</pmid><doi>10.1007/s00203-023-03641-6</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0302-8933 |
| ispartof | Archives of microbiology, 2023-09, Vol.205 (9), p.305-305, Article 305 |
| issn | 0302-8933 1432-072X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3040475545 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | acetates Acetic acid Amino acids Anaerobiosis Bacteria Bacteria, Anaerobic - genetics Bacterial Typing Techniques Base Composition Biochemistry biomass Biomedical and Life Sciences Biotechnology Butyric acid Carbohydrate metabolism Carbohydrates Carbon dioxide Cell Biology Cellulose Cellulose - metabolism Deoxyribonucleic acid DNA DNA, Bacterial - chemistry DNA, Bacterial - genetics Ecology Ethanol family Fatty acids Fatty Acids - analysis Formic acid Genes Genomes glucose Growth conditions Homology Hot springs Hot Springs - microbiology Hybridization Hydrogen Hydrogen - metabolism Hydrogen production India Lachnospiraceae Lactic acid Life Sciences Lignocellulose Microbial Ecology Microbiology New genera new genus New species Nucleotides Original Paper Phylogeny RNA, Ribosomal, 16S - genetics rRNA 16S Sediment samplers sediments Sequence Analysis, DNA Sodium chloride species Strain analysis Succinic acid Xylan Xylans |
| title | Description of Sporanaerobium hydrogeniformans gen. nov., sp. nov., an obligately anaerobic, hydrogen-producing bacterium isolated from Aravali hot spring in India |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T18%3A17%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Description%20of%20Sporanaerobium%20hydrogeniformans%20gen.%20nov.,%20sp.%20nov.,%20an%20obligately%20anaerobic,%20hydrogen-producing%20bacterium%20isolated%20from%20Aravali%20hot%20spring%20in%20India&rft.jtitle=Archives%20of%20microbiology&rft.au=Hivarkar,%20Sai%20Suresh&rft.date=2023-09-01&rft.volume=205&rft.issue=9&rft.spage=305&rft.epage=305&rft.pages=305-305&rft.artnum=305&rft.issn=0302-8933&rft.eissn=1432-072X&rft_id=info:doi/10.1007/s00203-023-03641-6&rft_dat=%3Cproquest_cross%3E2849391409%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2849391409&rft_id=info:pmid/37572166&rfr_iscdi=true |