Identification and characteristic analysis of an extracellular signal‐regulated kinase from Ostrinia furnacalis Guenée
The extracellular signal‐regulated kinase (ERK) pathway, a critical genetic determinant, controls diverse physiological functions, including innate immunity, development, and stress response. In the current study, a full‐length cDNA (1592bp) encoding the ERK gene (OfERK) was cloned from Ostrinia fur...
Gespeichert in:
| Veröffentlicht in: | Archives of insect biochemistry and physiology 2024-01, Vol.115 (1), p.e22077-n/a |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | n/a |
|---|---|
| container_issue | 1 |
| container_start_page | e22077 |
| container_title | Archives of insect biochemistry and physiology |
| container_volume | 115 |
| creator | Li, Shuzhong Xu, Fuqiang Zhang, Yiqiang Gao, Zupeng Han, Zhaoyang Feng, Congjing |
| description | The extracellular signal‐regulated kinase (ERK) pathway, a critical genetic determinant, controls diverse physiological functions, including innate immunity, development, and stress response. In the current study, a full‐length cDNA (1592bp) encoding the ERK gene (OfERK) was cloned from Ostrinia furnacalis Guenée (GenBank accession number: MF797866). The open reading frame of the OfERK gene encoded 364 amino acids and shared 96.43%–98.08% amino acid identities with other insect mitogen‐activated protein kinases. For spatiotemporal analysis of the expression pattern, OfERK exhibited a significant peak expression on the 3rd day of the pupa stage and showed the highest expression in hemocytes specifically. Indirect immunofluorescence assays and immuno‐electron microscopy revealed a wide distribution of the OfERK protein in hemocytes and epidermis. Moreover, the results demonstrated that the Bt Cry1Ab‐activated toxin significantly induces the expression of OfERK. Other genes related to immune response, development, and stress response exhibited dynamic changes in expression after Cry1Ab oral treatment. The expression of OfERK was downregulated through RNA interference, and the correlation of its expression with other related genes was verified using quantitative real‐time polymerase chain reaction. Our study provides valuable insights into the regulatory mechanism of ERK in insects for future studies.
The extracellular signal‐regulated kinase (ERK) pathway, a critical genetic determinant, controls diverse physiological functions, including innate immunity, development, and stress response. OfERK exhibited a significant peak expression on the 3rd day of the pupa stage and showed the highest expression in hemocytes specifically. Indirect immunofluorescence assays and immuno‐electron microscopy revealed a wide distribution of the OfERK protein in hemocytes and epidermis. The Bt Cry1Ab‐activated toxin significantly induces the expression of OfERK. Other genes related to immune response, development, and stress response exhibited dynamic changes in expression.
Highlights
OfERK was spatiotemporal specific high expressed on the third of pupa and in hemocytes, and the OfERK protein was widely distributed in hemocytes and epidermis.
Bt Cry1Ab significantly changed the expression of OfERK and other immune‐, developmental‐, and stress response‐related genes. |
| doi_str_mv | 10.1002/arch.22077 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3040389113</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3040389113</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3497-1633ee9982a65f5d5dc15d15ab053ebaf7958cdc77a0d431b6cda64382d637663</originalsourceid><addsrcrecordid>eNqFkctKHEEUhougxPGyyQOEBjcitNaluy5LGaIjCIKYdXOm6rSW9nSbqm7MuPIR8gjZ-hrmTfIkVjvGRRbJ6hzO-fjg5yfkE6MHjFJ-CMFeH3BOlfpAJqzkNJeCqzUyoUqYvCgk3yCbMd5QSo1k-iPZEJprXWgzIQ-nDtve195C77s2g9Zl9hoC2B6Dj7236QTNMvqYdXXaM_zepy82zdBAeP4Z_VX6_378EfAqXXp02a1vIWJWh26Rncc--NZDVg-hBQtN8pwM2P56wm2yXkMTcedtbpGvx18up7P87PzkdHp0lltRGJUzKQSiMZqDLOvSlc6y0rES5rQUOIdamVJbZ5UC6grB5tI6kEWK6KRQUootsrfy3oXu24CxrxY-jgGgxW6IlaAFFdowJv6LcsMp09Jok9Ddv9CbbozYjBQzSlKpR-H-irKhizFgXd0Fv4CwrBitxvKqsbzqtbwEf35TDvMFunf0T1sJYCvg3je4_IeqOrqYzlbSF2AfqCU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2919760683</pqid></control><display><type>article</type><title>Identification and characteristic analysis of an extracellular signal‐regulated kinase from Ostrinia furnacalis Guenée</title><source>Wiley Online Library Journals Frontfile Complete</source><creator>Li, Shuzhong ; Xu, Fuqiang ; Zhang, Yiqiang ; Gao, Zupeng ; Han, Zhaoyang ; Feng, Congjing</creator><creatorcontrib>Li, Shuzhong ; Xu, Fuqiang ; Zhang, Yiqiang ; Gao, Zupeng ; Han, Zhaoyang ; Feng, Congjing</creatorcontrib><description>The extracellular signal‐regulated kinase (ERK) pathway, a critical genetic determinant, controls diverse physiological functions, including innate immunity, development, and stress response. In the current study, a full‐length cDNA (1592bp) encoding the ERK gene (OfERK) was cloned from Ostrinia furnacalis Guenée (GenBank accession number: MF797866). The open reading frame of the OfERK gene encoded 364 amino acids and shared 96.43%–98.08% amino acid identities with other insect mitogen‐activated protein kinases. For spatiotemporal analysis of the expression pattern, OfERK exhibited a significant peak expression on the 3rd day of the pupa stage and showed the highest expression in hemocytes specifically. Indirect immunofluorescence assays and immuno‐electron microscopy revealed a wide distribution of the OfERK protein in hemocytes and epidermis. Moreover, the results demonstrated that the Bt Cry1Ab‐activated toxin significantly induces the expression of OfERK. Other genes related to immune response, development, and stress response exhibited dynamic changes in expression after Cry1Ab oral treatment. The expression of OfERK was downregulated through RNA interference, and the correlation of its expression with other related genes was verified using quantitative real‐time polymerase chain reaction. Our study provides valuable insights into the regulatory mechanism of ERK in insects for future studies.
The extracellular signal‐regulated kinase (ERK) pathway, a critical genetic determinant, controls diverse physiological functions, including innate immunity, development, and stress response. OfERK exhibited a significant peak expression on the 3rd day of the pupa stage and showed the highest expression in hemocytes specifically. Indirect immunofluorescence assays and immuno‐electron microscopy revealed a wide distribution of the OfERK protein in hemocytes and epidermis. The Bt Cry1Ab‐activated toxin significantly induces the expression of OfERK. Other genes related to immune response, development, and stress response exhibited dynamic changes in expression.
Highlights
OfERK was spatiotemporal specific high expressed on the third of pupa and in hemocytes, and the OfERK protein was widely distributed in hemocytes and epidermis.
Bt Cry1Ab significantly changed the expression of OfERK and other immune‐, developmental‐, and stress response‐related genes.</description><identifier>ISSN: 0739-4462</identifier><identifier>EISSN: 1520-6327</identifier><identifier>DOI: 10.1002/arch.22077</identifier><identifier>PMID: 38288489</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Amino acids ; Cellular stress response ; Cry1Ab toxin ; development ; Electron microscopy ; Epidermis ; Extracellular signal-regulated kinase ; fluorescent antibody technique ; Gene expression ; Genes ; Genetic diversity ; Hemocytes ; Identities ; Immune response ; Immune system ; Immunofluorescence ; Innate immunity ; insect biochemistry ; Insects ; Kinases ; microscopy ; mitogen-activated protein kinase ; oral administration ; Ostrinia furnacalis ; Polymerase chain reaction ; Proteins ; Pupae ; quantitative polymerase chain reaction ; Regulatory mechanisms (biology) ; RNA interference ; RNA-mediated interference ; stress response ; Toxins</subject><ispartof>Archives of insect biochemistry and physiology, 2024-01, Vol.115 (1), p.e22077-n/a</ispartof><rights>2023 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3497-1633ee9982a65f5d5dc15d15ab053ebaf7958cdc77a0d431b6cda64382d637663</cites><orcidid>0000-0001-7720-7956</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Farch.22077$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Farch.22077$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38288489$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Shuzhong</creatorcontrib><creatorcontrib>Xu, Fuqiang</creatorcontrib><creatorcontrib>Zhang, Yiqiang</creatorcontrib><creatorcontrib>Gao, Zupeng</creatorcontrib><creatorcontrib>Han, Zhaoyang</creatorcontrib><creatorcontrib>Feng, Congjing</creatorcontrib><title>Identification and characteristic analysis of an extracellular signal‐regulated kinase from Ostrinia furnacalis Guenée</title><title>Archives of insect biochemistry and physiology</title><addtitle>Arch Insect Biochem Physiol</addtitle><description>The extracellular signal‐regulated kinase (ERK) pathway, a critical genetic determinant, controls diverse physiological functions, including innate immunity, development, and stress response. In the current study, a full‐length cDNA (1592bp) encoding the ERK gene (OfERK) was cloned from Ostrinia furnacalis Guenée (GenBank accession number: MF797866). The open reading frame of the OfERK gene encoded 364 amino acids and shared 96.43%–98.08% amino acid identities with other insect mitogen‐activated protein kinases. For spatiotemporal analysis of the expression pattern, OfERK exhibited a significant peak expression on the 3rd day of the pupa stage and showed the highest expression in hemocytes specifically. Indirect immunofluorescence assays and immuno‐electron microscopy revealed a wide distribution of the OfERK protein in hemocytes and epidermis. Moreover, the results demonstrated that the Bt Cry1Ab‐activated toxin significantly induces the expression of OfERK. Other genes related to immune response, development, and stress response exhibited dynamic changes in expression after Cry1Ab oral treatment. The expression of OfERK was downregulated through RNA interference, and the correlation of its expression with other related genes was verified using quantitative real‐time polymerase chain reaction. Our study provides valuable insights into the regulatory mechanism of ERK in insects for future studies.
The extracellular signal‐regulated kinase (ERK) pathway, a critical genetic determinant, controls diverse physiological functions, including innate immunity, development, and stress response. OfERK exhibited a significant peak expression on the 3rd day of the pupa stage and showed the highest expression in hemocytes specifically. Indirect immunofluorescence assays and immuno‐electron microscopy revealed a wide distribution of the OfERK protein in hemocytes and epidermis. The Bt Cry1Ab‐activated toxin significantly induces the expression of OfERK. Other genes related to immune response, development, and stress response exhibited dynamic changes in expression.
Highlights
OfERK was spatiotemporal specific high expressed on the third of pupa and in hemocytes, and the OfERK protein was widely distributed in hemocytes and epidermis.
Bt Cry1Ab significantly changed the expression of OfERK and other immune‐, developmental‐, and stress response‐related genes.</description><subject>Amino acids</subject><subject>Cellular stress response</subject><subject>Cry1Ab toxin</subject><subject>development</subject><subject>Electron microscopy</subject><subject>Epidermis</subject><subject>Extracellular signal-regulated kinase</subject><subject>fluorescent antibody technique</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic diversity</subject><subject>Hemocytes</subject><subject>Identities</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunofluorescence</subject><subject>Innate immunity</subject><subject>insect biochemistry</subject><subject>Insects</subject><subject>Kinases</subject><subject>microscopy</subject><subject>mitogen-activated protein kinase</subject><subject>oral administration</subject><subject>Ostrinia furnacalis</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Pupae</subject><subject>quantitative polymerase chain reaction</subject><subject>Regulatory mechanisms (biology)</subject><subject>RNA interference</subject><subject>RNA-mediated interference</subject><subject>stress response</subject><subject>Toxins</subject><issn>0739-4462</issn><issn>1520-6327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkctKHEEUhougxPGyyQOEBjcitNaluy5LGaIjCIKYdXOm6rSW9nSbqm7MuPIR8gjZ-hrmTfIkVjvGRRbJ6hzO-fjg5yfkE6MHjFJ-CMFeH3BOlfpAJqzkNJeCqzUyoUqYvCgk3yCbMd5QSo1k-iPZEJprXWgzIQ-nDtve195C77s2g9Zl9hoC2B6Dj7236QTNMvqYdXXaM_zepy82zdBAeP4Z_VX6_378EfAqXXp02a1vIWJWh26Rncc--NZDVg-hBQtN8pwM2P56wm2yXkMTcedtbpGvx18up7P87PzkdHp0lltRGJUzKQSiMZqDLOvSlc6y0rES5rQUOIdamVJbZ5UC6grB5tI6kEWK6KRQUootsrfy3oXu24CxrxY-jgGgxW6IlaAFFdowJv6LcsMp09Jok9Ddv9CbbozYjBQzSlKpR-H-irKhizFgXd0Fv4CwrBitxvKqsbzqtbwEf35TDvMFunf0T1sJYCvg3je4_IeqOrqYzlbSF2AfqCU</recordid><startdate>202401</startdate><enddate>202401</enddate><creator>Li, Shuzhong</creator><creator>Xu, Fuqiang</creator><creator>Zhang, Yiqiang</creator><creator>Gao, Zupeng</creator><creator>Han, Zhaoyang</creator><creator>Feng, Congjing</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-7720-7956</orcidid></search><sort><creationdate>202401</creationdate><title>Identification and characteristic analysis of an extracellular signal‐regulated kinase from Ostrinia furnacalis Guenée</title><author>Li, Shuzhong ; Xu, Fuqiang ; Zhang, Yiqiang ; Gao, Zupeng ; Han, Zhaoyang ; Feng, Congjing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3497-1633ee9982a65f5d5dc15d15ab053ebaf7958cdc77a0d431b6cda64382d637663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Amino acids</topic><topic>Cellular stress response</topic><topic>Cry1Ab toxin</topic><topic>development</topic><topic>Electron microscopy</topic><topic>Epidermis</topic><topic>Extracellular signal-regulated kinase</topic><topic>fluorescent antibody technique</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genetic diversity</topic><topic>Hemocytes</topic><topic>Identities</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Immunofluorescence</topic><topic>Innate immunity</topic><topic>insect biochemistry</topic><topic>Insects</topic><topic>Kinases</topic><topic>microscopy</topic><topic>mitogen-activated protein kinase</topic><topic>oral administration</topic><topic>Ostrinia furnacalis</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Pupae</topic><topic>quantitative polymerase chain reaction</topic><topic>Regulatory mechanisms (biology)</topic><topic>RNA interference</topic><topic>RNA-mediated interference</topic><topic>stress response</topic><topic>Toxins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Shuzhong</creatorcontrib><creatorcontrib>Xu, Fuqiang</creatorcontrib><creatorcontrib>Zhang, Yiqiang</creatorcontrib><creatorcontrib>Gao, Zupeng</creatorcontrib><creatorcontrib>Han, Zhaoyang</creatorcontrib><creatorcontrib>Feng, Congjing</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Archives of insect biochemistry and physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Shuzhong</au><au>Xu, Fuqiang</au><au>Zhang, Yiqiang</au><au>Gao, Zupeng</au><au>Han, Zhaoyang</au><au>Feng, Congjing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characteristic analysis of an extracellular signal‐regulated kinase from Ostrinia furnacalis Guenée</atitle><jtitle>Archives of insect biochemistry and physiology</jtitle><addtitle>Arch Insect Biochem Physiol</addtitle><date>2024-01</date><risdate>2024</risdate><volume>115</volume><issue>1</issue><spage>e22077</spage><epage>n/a</epage><pages>e22077-n/a</pages><issn>0739-4462</issn><eissn>1520-6327</eissn><abstract>The extracellular signal‐regulated kinase (ERK) pathway, a critical genetic determinant, controls diverse physiological functions, including innate immunity, development, and stress response. In the current study, a full‐length cDNA (1592bp) encoding the ERK gene (OfERK) was cloned from Ostrinia furnacalis Guenée (GenBank accession number: MF797866). The open reading frame of the OfERK gene encoded 364 amino acids and shared 96.43%–98.08% amino acid identities with other insect mitogen‐activated protein kinases. For spatiotemporal analysis of the expression pattern, OfERK exhibited a significant peak expression on the 3rd day of the pupa stage and showed the highest expression in hemocytes specifically. Indirect immunofluorescence assays and immuno‐electron microscopy revealed a wide distribution of the OfERK protein in hemocytes and epidermis. Moreover, the results demonstrated that the Bt Cry1Ab‐activated toxin significantly induces the expression of OfERK. Other genes related to immune response, development, and stress response exhibited dynamic changes in expression after Cry1Ab oral treatment. The expression of OfERK was downregulated through RNA interference, and the correlation of its expression with other related genes was verified using quantitative real‐time polymerase chain reaction. Our study provides valuable insights into the regulatory mechanism of ERK in insects for future studies.
The extracellular signal‐regulated kinase (ERK) pathway, a critical genetic determinant, controls diverse physiological functions, including innate immunity, development, and stress response. OfERK exhibited a significant peak expression on the 3rd day of the pupa stage and showed the highest expression in hemocytes specifically. Indirect immunofluorescence assays and immuno‐electron microscopy revealed a wide distribution of the OfERK protein in hemocytes and epidermis. The Bt Cry1Ab‐activated toxin significantly induces the expression of OfERK. Other genes related to immune response, development, and stress response exhibited dynamic changes in expression.
Highlights
OfERK was spatiotemporal specific high expressed on the third of pupa and in hemocytes, and the OfERK protein was widely distributed in hemocytes and epidermis.
Bt Cry1Ab significantly changed the expression of OfERK and other immune‐, developmental‐, and stress response‐related genes.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>38288489</pmid><doi>10.1002/arch.22077</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0001-7720-7956</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0739-4462 |
| ispartof | Archives of insect biochemistry and physiology, 2024-01, Vol.115 (1), p.e22077-n/a |
| issn | 0739-4462 1520-6327 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3040389113 |
| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Amino acids Cellular stress response Cry1Ab toxin development Electron microscopy Epidermis Extracellular signal-regulated kinase fluorescent antibody technique Gene expression Genes Genetic diversity Hemocytes Identities Immune response Immune system Immunofluorescence Innate immunity insect biochemistry Insects Kinases microscopy mitogen-activated protein kinase oral administration Ostrinia furnacalis Polymerase chain reaction Proteins Pupae quantitative polymerase chain reaction Regulatory mechanisms (biology) RNA interference RNA-mediated interference stress response Toxins |
| title | Identification and characteristic analysis of an extracellular signal‐regulated kinase from Ostrinia furnacalis Guenée |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T20%3A24%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20and%20characteristic%20analysis%20of%20an%20extracellular%C2%A0signal%E2%80%90regulated%20kinase%20from%20Ostrinia%20furnacalis%20Guen%C3%A9e&rft.jtitle=Archives%20of%20insect%20biochemistry%20and%20physiology&rft.au=Li,%20Shuzhong&rft.date=2024-01&rft.volume=115&rft.issue=1&rft.spage=e22077&rft.epage=n/a&rft.pages=e22077-n/a&rft.issn=0739-4462&rft.eissn=1520-6327&rft_id=info:doi/10.1002/arch.22077&rft_dat=%3Cproquest_cross%3E3040389113%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2919760683&rft_id=info:pmid/38288489&rfr_iscdi=true |