Size- and surface functionalization-driven molecular interaction of CdSe quantum dots with jack bean urease: multispectroscopic, thermodynamic, and AFM approach
Quantum dots (QDs) with distinctive optical properties have been extensively researched and developed for usage in solar cells, imaging, drug delivery, cellular targeting, etc. But the inevitable production of QDs can lead to their unavoidable release and increased environmental concentration. Depen...
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| description | Quantum dots (QDs) with distinctive optical properties have been extensively researched and developed for usage in solar cells, imaging, drug delivery, cellular targeting, etc. But the inevitable production of QDs can lead to their unavoidable release and increased environmental concentration. Depending on morphological and surface properties, QDs at the nano-bio interface considerably impact the activity and structure of bio-molecules. The present study investigates the interaction of metalloenzyme jack bean urease (JBU) and bi-sized CdSe QDs (2.43 nm and 3.63 nm), surface-functionalized to mercaptopropionic acid (MPA) (–COOH),
l
-cysteine (CYS),
l
-glutathione (GSH), N-acetyl
l
-cysteine (NAC) (–COOH, –NH
2
), and cysteamine hydrochloride (CYST) (–NH
2
) to assess any alterations in JBU’s binding, microenvironment, structure, exciton lifetime, and activity. JBU catalyzes the hydrolysis of urea to produce ammonia and carbon dioxide; any changes in its properties could threaten the survival of several microbes and plants. Spectroscopy techniques such as UV–Vis, fluorescence, circular dichroism, synchronous, time-resolved fluorescence, atomic force microscopy, and JBU activity assay were studied. Results suggested highly spontaneous and energy-favored interactions, which involved static quenching and hydrophobic forces of varied magnitude, dependent on QDs properties. The size, surface modifications, and dosage of QDs significantly impacted the secondary structure and activity of JBUs. Even though the larger sizes of the relevant modifications demonstrated stronger binding, the smaller sizes had the greatest impact on α-helicity and activity. CYST-capped QDs with an average number of the binding site (
n
) = 1, reduced α-helicity by 16% and activity by 22–30% at 7 nM concentration. In contrast, MPA-capped QDs with
n
|
| doi_str_mv | 10.1007/s11356-023-25356-3 |
| format | Article |
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l
-cysteine (CYS),
l
-glutathione (GSH), N-acetyl
l
-cysteine (NAC) (–COOH, –NH
2
), and cysteamine hydrochloride (CYST) (–NH
2
) to assess any alterations in JBU’s binding, microenvironment, structure, exciton lifetime, and activity. JBU catalyzes the hydrolysis of urea to produce ammonia and carbon dioxide; any changes in its properties could threaten the survival of several microbes and plants. Spectroscopy techniques such as UV–Vis, fluorescence, circular dichroism, synchronous, time-resolved fluorescence, atomic force microscopy, and JBU activity assay were studied. Results suggested highly spontaneous and energy-favored interactions, which involved static quenching and hydrophobic forces of varied magnitude, dependent on QDs properties. The size, surface modifications, and dosage of QDs significantly impacted the secondary structure and activity of JBUs. Even though the larger sizes of the relevant modifications demonstrated stronger binding, the smaller sizes had the greatest impact on α-helicity and activity. CYST-capped QDs with an average number of the binding site (
n
) = 1, reduced α-helicity by 16% and activity by 22–30% at 7 nM concentration. In contrast, MPA-capped QDs with
n
< 1 had the least effect on α-helical structure and activity. The smaller GSH-capped QDs increased the activity by 9%, via partially restoring JBU’s α-helical content. The study thus thoroughly analyzed the impact of varied-size and surface-functionalized QDs on the structure and function of JBU, which can be exploited further for several biomedical applications.
Graphical Abstract</description><identifier>ISSN: 1614-7499</identifier><identifier>ISSN: 0944-1344</identifier><identifier>EISSN: 1614-7499</identifier><identifier>DOI: 10.1007/s11356-023-25356-3</identifier><identifier>PMID: 36757588</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acetylcysteine ; Ammonia ; Aquatic Pollution ; Atmospheric Protection/Air Quality Control/Air Pollution ; Atomic force microscopy ; Beans ; Binding sites ; Biomedical materials ; Biomolecules ; Cadmium Compounds - chemistry ; Canavalia ensiformis ; Carbon dioxide ; Circular dichroism ; circular dichroism spectroscopy ; Cysteamine ; Cysteine ; Cysts ; Dichroism ; Drug delivery ; drugs ; Earth and Environmental Science ; Ecotoxicology ; Environment ; Environmental Chemistry ; Environmental Health ; Environmental science ; Excitons ; Fluorescence ; Glutathione ; Helicity ; Humans ; hydrolysis ; Hydrophobicity ; Impact analysis ; Jack beans ; Microenvironments ; Molecular interactions ; Molecular structure ; Optical properties ; Photovoltaic cells ; Protein structure ; Quantum dots ; Quantum Dots - chemistry ; Research Article ; Secondary structure ; Selenium Compounds - chemistry ; Solar cells ; Spectroscopy ; Structure-function relationships ; Surface properties ; Thermodynamics ; Tumor Microenvironment ; Urea ; Urease ; Urease - metabolism ; Waste Water Technology ; Water Management ; Water Pollution Control</subject><ispartof>Environmental science and pollution research international, 2023-04, Vol.30 (16), p.48300-48322</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-338e1b36ac4ceca63c933ab7b8e113339a8195ea4a9bb8f5a7f503c79db9de1c3</citedby><cites>FETCH-LOGICAL-c408t-338e1b36ac4ceca63c933ab7b8e113339a8195ea4a9bb8f5a7f503c79db9de1c3</cites><orcidid>0000-0003-4922-9028</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11356-023-25356-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11356-023-25356-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36757588$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gupta, Jagriti</creatorcontrib><creatorcontrib>Rajamani, Paulraj</creatorcontrib><title>Size- and surface functionalization-driven molecular interaction of CdSe quantum dots with jack bean urease: multispectroscopic, thermodynamic, and AFM approach</title><title>Environmental science and pollution research international</title><addtitle>Environ Sci Pollut Res</addtitle><addtitle>Environ Sci Pollut Res Int</addtitle><description>Quantum dots (QDs) with distinctive optical properties have been extensively researched and developed for usage in solar cells, imaging, drug delivery, cellular targeting, etc. But the inevitable production of QDs can lead to their unavoidable release and increased environmental concentration. Depending on morphological and surface properties, QDs at the nano-bio interface considerably impact the activity and structure of bio-molecules. The present study investigates the interaction of metalloenzyme jack bean urease (JBU) and bi-sized CdSe QDs (2.43 nm and 3.63 nm), surface-functionalized to mercaptopropionic acid (MPA) (–COOH),
l
-cysteine (CYS),
l
-glutathione (GSH), N-acetyl
l
-cysteine (NAC) (–COOH, –NH
2
), and cysteamine hydrochloride (CYST) (–NH
2
) to assess any alterations in JBU’s binding, microenvironment, structure, exciton lifetime, and activity. JBU catalyzes the hydrolysis of urea to produce ammonia and carbon dioxide; any changes in its properties could threaten the survival of several microbes and plants. Spectroscopy techniques such as UV–Vis, fluorescence, circular dichroism, synchronous, time-resolved fluorescence, atomic force microscopy, and JBU activity assay were studied. Results suggested highly spontaneous and energy-favored interactions, which involved static quenching and hydrophobic forces of varied magnitude, dependent on QDs properties. The size, surface modifications, and dosage of QDs significantly impacted the secondary structure and activity of JBUs. Even though the larger sizes of the relevant modifications demonstrated stronger binding, the smaller sizes had the greatest impact on α-helicity and activity. CYST-capped QDs with an average number of the binding site (
n
) = 1, reduced α-helicity by 16% and activity by 22–30% at 7 nM concentration. In contrast, MPA-capped QDs with
n
< 1 had the least effect on α-helical structure and activity. The smaller GSH-capped QDs increased the activity by 9%, via partially restoring JBU’s α-helical content. The study thus thoroughly analyzed the impact of varied-size and surface-functionalized QDs on the structure and function of JBU, which can be exploited further for several biomedical applications.
Graphical Abstract</description><subject>Acetylcysteine</subject><subject>Ammonia</subject><subject>Aquatic Pollution</subject><subject>Atmospheric Protection/Air Quality Control/Air Pollution</subject><subject>Atomic force microscopy</subject><subject>Beans</subject><subject>Binding sites</subject><subject>Biomedical materials</subject><subject>Biomolecules</subject><subject>Cadmium Compounds - chemistry</subject><subject>Canavalia ensiformis</subject><subject>Carbon dioxide</subject><subject>Circular dichroism</subject><subject>circular dichroism spectroscopy</subject><subject>Cysteamine</subject><subject>Cysteine</subject><subject>Cysts</subject><subject>Dichroism</subject><subject>Drug delivery</subject><subject>drugs</subject><subject>Earth and Environmental Science</subject><subject>Ecotoxicology</subject><subject>Environment</subject><subject>Environmental Chemistry</subject><subject>Environmental Health</subject><subject>Environmental science</subject><subject>Excitons</subject><subject>Fluorescence</subject><subject>Glutathione</subject><subject>Helicity</subject><subject>Humans</subject><subject>hydrolysis</subject><subject>Hydrophobicity</subject><subject>Impact analysis</subject><subject>Jack beans</subject><subject>Microenvironments</subject><subject>Molecular interactions</subject><subject>Molecular structure</subject><subject>Optical properties</subject><subject>Photovoltaic cells</subject><subject>Protein structure</subject><subject>Quantum dots</subject><subject>Quantum Dots - chemistry</subject><subject>Research Article</subject><subject>Secondary structure</subject><subject>Selenium Compounds - chemistry</subject><subject>Solar cells</subject><subject>Spectroscopy</subject><subject>Structure-function relationships</subject><subject>Surface properties</subject><subject>Thermodynamics</subject><subject>Tumor Microenvironment</subject><subject>Urea</subject><subject>Urease</subject><subject>Urease - metabolism</subject><subject>Waste Water Technology</subject><subject>Water Management</subject><subject>Water Pollution Control</subject><issn>1614-7499</issn><issn>0944-1344</issn><issn>1614-7499</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkctu1TAQhiMEouXAC7BAltiwaMCOk9hmVx1RQCpiUVhHE2fC8SGxU19A7dP0UXF6ykUsYOWx_fkfa76ieMroS0apeBUY401b0oqXVbNW_F5xzFpWl6JW6v4f9VHxKIQ9pRVVlXhYHPFWNKKR8ri4uTDXWBKwAwnJj6CRjMnqaJyFyVzDWpSDN9_QktlNqNMEnhgb0cMtRdxItsMFkssENqaZDC4G8t3EHdmD_kp6BEuSRwj4msxpiiYsqKN3QbvF6BMSd-hnN1xZmNft-pPTsw8ElsU70LvHxYMRpoBP7tZN8fnszaftu_L849v329PzUtdUxpJziaznLehao4aWa8U59KLPx4xzrkAy1SDUoPpejg2IsaFcCzX0akCm-aZ4ccjNbS8ThtjNJmicJrDoUug4rSkXLc1Z_0MrIWqpZCVX9Plf6N4ln0ebKUmFaqXI6jZFdaB0HkvwOHaLNzP4q47RblXdHVR3WXV3q7pbo5_dRad-xuHXk59uM8APQMhX9gv6373_EfsDcRS3GA</recordid><startdate>20230401</startdate><enddate>20230401</enddate><creator>Gupta, Jagriti</creator><creator>Rajamani, Paulraj</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SN</scope><scope>7T7</scope><scope>7TV</scope><scope>7U7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>P64</scope><scope>PATMY</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0003-4922-9028</orcidid></search><sort><creationdate>20230401</creationdate><title>Size- and surface functionalization-driven molecular interaction of CdSe quantum dots with jack bean urease: multispectroscopic, thermodynamic, and AFM approach</title><author>Gupta, Jagriti ; Rajamani, Paulraj</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-338e1b36ac4ceca63c933ab7b8e113339a8195ea4a9bb8f5a7f503c79db9de1c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Acetylcysteine</topic><topic>Ammonia</topic><topic>Aquatic Pollution</topic><topic>Atmospheric Protection/Air Quality Control/Air Pollution</topic><topic>Atomic force microscopy</topic><topic>Beans</topic><topic>Binding sites</topic><topic>Biomedical materials</topic><topic>Biomolecules</topic><topic>Cadmium Compounds - chemistry</topic><topic>Canavalia ensiformis</topic><topic>Carbon dioxide</topic><topic>Circular dichroism</topic><topic>circular dichroism spectroscopy</topic><topic>Cysteamine</topic><topic>Cysteine</topic><topic>Cysts</topic><topic>Dichroism</topic><topic>Drug delivery</topic><topic>drugs</topic><topic>Earth and Environmental Science</topic><topic>Ecotoxicology</topic><topic>Environment</topic><topic>Environmental Chemistry</topic><topic>Environmental Health</topic><topic>Environmental science</topic><topic>Excitons</topic><topic>Fluorescence</topic><topic>Glutathione</topic><topic>Helicity</topic><topic>Humans</topic><topic>hydrolysis</topic><topic>Hydrophobicity</topic><topic>Impact analysis</topic><topic>Jack beans</topic><topic>Microenvironments</topic><topic>Molecular interactions</topic><topic>Molecular structure</topic><topic>Optical properties</topic><topic>Photovoltaic cells</topic><topic>Protein structure</topic><topic>Quantum dots</topic><topic>Quantum Dots - chemistry</topic><topic>Research Article</topic><topic>Secondary structure</topic><topic>Selenium Compounds - chemistry</topic><topic>Solar cells</topic><topic>Spectroscopy</topic><topic>Structure-function relationships</topic><topic>Surface properties</topic><topic>Thermodynamics</topic><topic>Tumor Microenvironment</topic><topic>Urea</topic><topic>Urease</topic><topic>Urease - metabolism</topic><topic>Waste Water Technology</topic><topic>Water Management</topic><topic>Water Pollution Control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gupta, Jagriti</creatorcontrib><creatorcontrib>Rajamani, Paulraj</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Environmental science and pollution research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gupta, Jagriti</au><au>Rajamani, Paulraj</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Size- and surface functionalization-driven molecular interaction of CdSe quantum dots with jack bean urease: multispectroscopic, thermodynamic, and AFM approach</atitle><jtitle>Environmental science and pollution research international</jtitle><stitle>Environ Sci Pollut Res</stitle><addtitle>Environ Sci Pollut Res Int</addtitle><date>2023-04-01</date><risdate>2023</risdate><volume>30</volume><issue>16</issue><spage>48300</spage><epage>48322</epage><pages>48300-48322</pages><issn>1614-7499</issn><issn>0944-1344</issn><eissn>1614-7499</eissn><abstract>Quantum dots (QDs) with distinctive optical properties have been extensively researched and developed for usage in solar cells, imaging, drug delivery, cellular targeting, etc. But the inevitable production of QDs can lead to their unavoidable release and increased environmental concentration. Depending on morphological and surface properties, QDs at the nano-bio interface considerably impact the activity and structure of bio-molecules. The present study investigates the interaction of metalloenzyme jack bean urease (JBU) and bi-sized CdSe QDs (2.43 nm and 3.63 nm), surface-functionalized to mercaptopropionic acid (MPA) (–COOH),
l
-cysteine (CYS),
l
-glutathione (GSH), N-acetyl
l
-cysteine (NAC) (–COOH, –NH
2
), and cysteamine hydrochloride (CYST) (–NH
2
) to assess any alterations in JBU’s binding, microenvironment, structure, exciton lifetime, and activity. JBU catalyzes the hydrolysis of urea to produce ammonia and carbon dioxide; any changes in its properties could threaten the survival of several microbes and plants. Spectroscopy techniques such as UV–Vis, fluorescence, circular dichroism, synchronous, time-resolved fluorescence, atomic force microscopy, and JBU activity assay were studied. Results suggested highly spontaneous and energy-favored interactions, which involved static quenching and hydrophobic forces of varied magnitude, dependent on QDs properties. The size, surface modifications, and dosage of QDs significantly impacted the secondary structure and activity of JBUs. Even though the larger sizes of the relevant modifications demonstrated stronger binding, the smaller sizes had the greatest impact on α-helicity and activity. CYST-capped QDs with an average number of the binding site (
n
) = 1, reduced α-helicity by 16% and activity by 22–30% at 7 nM concentration. In contrast, MPA-capped QDs with
n
< 1 had the least effect on α-helical structure and activity. The smaller GSH-capped QDs increased the activity by 9%, via partially restoring JBU’s α-helical content. The study thus thoroughly analyzed the impact of varied-size and surface-functionalized QDs on the structure and function of JBU, which can be exploited further for several biomedical applications.
Graphical Abstract</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>36757588</pmid><doi>10.1007/s11356-023-25356-3</doi><tpages>23</tpages><orcidid>https://orcid.org/0000-0003-4922-9028</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1614-7499 |
| ispartof | Environmental science and pollution research international, 2023-04, Vol.30 (16), p.48300-48322 |
| issn | 1614-7499 0944-1344 1614-7499 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3040376033 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Acetylcysteine Ammonia Aquatic Pollution Atmospheric Protection/Air Quality Control/Air Pollution Atomic force microscopy Beans Binding sites Biomedical materials Biomolecules Cadmium Compounds - chemistry Canavalia ensiformis Carbon dioxide Circular dichroism circular dichroism spectroscopy Cysteamine Cysteine Cysts Dichroism Drug delivery drugs Earth and Environmental Science Ecotoxicology Environment Environmental Chemistry Environmental Health Environmental science Excitons Fluorescence Glutathione Helicity Humans hydrolysis Hydrophobicity Impact analysis Jack beans Microenvironments Molecular interactions Molecular structure Optical properties Photovoltaic cells Protein structure Quantum dots Quantum Dots - chemistry Research Article Secondary structure Selenium Compounds - chemistry Solar cells Spectroscopy Structure-function relationships Surface properties Thermodynamics Tumor Microenvironment Urea Urease Urease - metabolism Waste Water Technology Water Management Water Pollution Control |
| title | Size- and surface functionalization-driven molecular interaction of CdSe quantum dots with jack bean urease: multispectroscopic, thermodynamic, and AFM approach |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-16T11%3A11%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Size-%20and%20surface%20functionalization-driven%20molecular%20interaction%20of%20CdSe%20quantum%20dots%20with%20jack%20bean%20urease:%20multispectroscopic,%20thermodynamic,%20and%20AFM%20approach&rft.jtitle=Environmental%20science%20and%20pollution%20research%20international&rft.au=Gupta,%20Jagriti&rft.date=2023-04-01&rft.volume=30&rft.issue=16&rft.spage=48300&rft.epage=48322&rft.pages=48300-48322&rft.issn=1614-7499&rft.eissn=1614-7499&rft_id=info:doi/10.1007/s11356-023-25356-3&rft_dat=%3Cproquest_cross%3E2807968725%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2807968725&rft_id=info:pmid/36757588&rfr_iscdi=true |