Liquid Biopsy for Detection of Pancreaticobiliary Cancers by Functional Enrichment and Immunofluorescent Profiling of Circulating Tumor Cells and Their Clusters
Circulating tumor cells (CTCs) have historically been used for prognostication in oncology. We evaluate the performance of liquid biopsy CTC assay as a diagnostic tool in suspected pancreaticobiliary cancers (PBC). The assay utilizes functional enrichment of CTCs followed by immunofluorescent profil...
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| Veröffentlicht in: | Cancers 2024-04, Vol.16 (7), p.1400 |
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| creator | Gaya, Andrew Rohatgi, Nitesh Limaye, Sewanti Shreenivas, Aditya Ajami, Ramin Akolkar, Dadasaheb Datta, Vineet Srinivasan, Ajay Patil, Darshana |
| description | Circulating tumor cells (CTCs) have historically been used for prognostication in oncology. We evaluate the performance of liquid biopsy CTC assay as a diagnostic tool in suspected pancreaticobiliary cancers (PBC). The assay utilizes functional enrichment of CTCs followed by immunofluorescent profiling of organ-specific markers. The performance of the assay was first evaluated in a multicentric case-control study of blood samples from 360 participants, including 188 PBC cases (pre-biopsy samples) and 172 healthy individuals. A subsequent prospective observational study included pre-biopsy blood samples from 88 individuals with suspicion of PBC and no prior diagnosis of cancer. CTCs were harvested using a unique functional enrichment method and used for immunofluorescent profiling for CA19.9, Maspin, EpCAM, CK, and CD45, blinded to the tissue histopathological diagnosis. TruBlood
malignant or non-malignant predictions were compared with tissue diagnoses to establish sensitivity and specificity. The test had 95.9% overall sensitivity (95% CI: 86.0-99.5%) and 92.3% specificity (95% CI: 79.13% to 98.38%) to differentiate PBC (n = 49) from benign conditions (n = 39). The high accuracy of the CTC-based TruBlood test demonstrates its potential clinical application as a diagnostic tool to assist the effective detection of PBC when tissue sampling is unviable or inconclusive. |
| doi_str_mv | 10.3390/cancers16071400 |
| format | Article |
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malignant or non-malignant predictions were compared with tissue diagnoses to establish sensitivity and specificity. The test had 95.9% overall sensitivity (95% CI: 86.0-99.5%) and 92.3% specificity (95% CI: 79.13% to 98.38%) to differentiate PBC (n = 49) from benign conditions (n = 39). The high accuracy of the CTC-based TruBlood test demonstrates its potential clinical application as a diagnostic tool to assist the effective detection of PBC when tissue sampling is unviable or inconclusive.</description><identifier>ISSN: 2072-6694</identifier><identifier>EISSN: 2072-6694</identifier><identifier>DOI: 10.3390/cancers16071400</identifier><identifier>PMID: 38611078</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Abdomen ; Antigens ; Asymptomatic ; Bile ducts ; Biopsy ; Blood ; Cancer ; CD45 antigen ; Diagnosis ; Endoscopy ; Ethylenediaminetetraacetic acid ; Medical examination ; Medical prognosis ; Metastasis ; Observational studies ; Pancreatic cancer ; Patients ; Sampling ; Tumor cells ; Tumors</subject><ispartof>Cancers, 2024-04, Vol.16 (7), p.1400</ispartof><rights>COPYRIGHT 2024 MDPI AG</rights><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c387t-3ad9156739a53d338d7bf6e4378e629e4828bc7c2b9d26cac98e7beb2a8d8a223</cites><orcidid>0000-0002-4434-488X ; 0000-0002-3145-9469</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38611078$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gaya, Andrew</creatorcontrib><creatorcontrib>Rohatgi, Nitesh</creatorcontrib><creatorcontrib>Limaye, Sewanti</creatorcontrib><creatorcontrib>Shreenivas, Aditya</creatorcontrib><creatorcontrib>Ajami, Ramin</creatorcontrib><creatorcontrib>Akolkar, Dadasaheb</creatorcontrib><creatorcontrib>Datta, Vineet</creatorcontrib><creatorcontrib>Srinivasan, Ajay</creatorcontrib><creatorcontrib>Patil, Darshana</creatorcontrib><title>Liquid Biopsy for Detection of Pancreaticobiliary Cancers by Functional Enrichment and Immunofluorescent Profiling of Circulating Tumor Cells and Their Clusters</title><title>Cancers</title><addtitle>Cancers (Basel)</addtitle><description>Circulating tumor cells (CTCs) have historically been used for prognostication in oncology. We evaluate the performance of liquid biopsy CTC assay as a diagnostic tool in suspected pancreaticobiliary cancers (PBC). The assay utilizes functional enrichment of CTCs followed by immunofluorescent profiling of organ-specific markers. The performance of the assay was first evaluated in a multicentric case-control study of blood samples from 360 participants, including 188 PBC cases (pre-biopsy samples) and 172 healthy individuals. A subsequent prospective observational study included pre-biopsy blood samples from 88 individuals with suspicion of PBC and no prior diagnosis of cancer. CTCs were harvested using a unique functional enrichment method and used for immunofluorescent profiling for CA19.9, Maspin, EpCAM, CK, and CD45, blinded to the tissue histopathological diagnosis. TruBlood
malignant or non-malignant predictions were compared with tissue diagnoses to establish sensitivity and specificity. The test had 95.9% overall sensitivity (95% CI: 86.0-99.5%) and 92.3% specificity (95% CI: 79.13% to 98.38%) to differentiate PBC (n = 49) from benign conditions (n = 39). 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Rohatgi, Nitesh ; Limaye, Sewanti ; Shreenivas, Aditya ; Ajami, Ramin ; Akolkar, Dadasaheb ; Datta, Vineet ; Srinivasan, Ajay ; Patil, Darshana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-3ad9156739a53d338d7bf6e4378e629e4828bc7c2b9d26cac98e7beb2a8d8a223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Abdomen</topic><topic>Antigens</topic><topic>Asymptomatic</topic><topic>Bile ducts</topic><topic>Biopsy</topic><topic>Blood</topic><topic>Cancer</topic><topic>CD45 antigen</topic><topic>Diagnosis</topic><topic>Endoscopy</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Medical examination</topic><topic>Medical prognosis</topic><topic>Metastasis</topic><topic>Observational studies</topic><topic>Pancreatic cancer</topic><topic>Patients</topic><topic>Sampling</topic><topic>Tumor cells</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gaya, Andrew</creatorcontrib><creatorcontrib>Rohatgi, Nitesh</creatorcontrib><creatorcontrib>Limaye, Sewanti</creatorcontrib><creatorcontrib>Shreenivas, Aditya</creatorcontrib><creatorcontrib>Ajami, Ramin</creatorcontrib><creatorcontrib>Akolkar, Dadasaheb</creatorcontrib><creatorcontrib>Datta, Vineet</creatorcontrib><creatorcontrib>Srinivasan, Ajay</creatorcontrib><creatorcontrib>Patil, Darshana</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Cancers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gaya, Andrew</au><au>Rohatgi, Nitesh</au><au>Limaye, Sewanti</au><au>Shreenivas, Aditya</au><au>Ajami, Ramin</au><au>Akolkar, Dadasaheb</au><au>Datta, Vineet</au><au>Srinivasan, Ajay</au><au>Patil, Darshana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Liquid Biopsy for Detection of Pancreaticobiliary Cancers by Functional Enrichment and Immunofluorescent Profiling of Circulating Tumor Cells and Their Clusters</atitle><jtitle>Cancers</jtitle><addtitle>Cancers (Basel)</addtitle><date>2024-04-01</date><risdate>2024</risdate><volume>16</volume><issue>7</issue><spage>1400</spage><pages>1400-</pages><issn>2072-6694</issn><eissn>2072-6694</eissn><abstract>Circulating tumor cells (CTCs) have historically been used for prognostication in oncology. We evaluate the performance of liquid biopsy CTC assay as a diagnostic tool in suspected pancreaticobiliary cancers (PBC). The assay utilizes functional enrichment of CTCs followed by immunofluorescent profiling of organ-specific markers. The performance of the assay was first evaluated in a multicentric case-control study of blood samples from 360 participants, including 188 PBC cases (pre-biopsy samples) and 172 healthy individuals. A subsequent prospective observational study included pre-biopsy blood samples from 88 individuals with suspicion of PBC and no prior diagnosis of cancer. CTCs were harvested using a unique functional enrichment method and used for immunofluorescent profiling for CA19.9, Maspin, EpCAM, CK, and CD45, blinded to the tissue histopathological diagnosis. TruBlood
malignant or non-malignant predictions were compared with tissue diagnoses to establish sensitivity and specificity. The test had 95.9% overall sensitivity (95% CI: 86.0-99.5%) and 92.3% specificity (95% CI: 79.13% to 98.38%) to differentiate PBC (n = 49) from benign conditions (n = 39). The high accuracy of the CTC-based TruBlood test demonstrates its potential clinical application as a diagnostic tool to assist the effective detection of PBC when tissue sampling is unviable or inconclusive.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>38611078</pmid><doi>10.3390/cancers16071400</doi><orcidid>https://orcid.org/0000-0002-4434-488X</orcidid><orcidid>https://orcid.org/0000-0002-3145-9469</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Abdomen Antigens Asymptomatic Bile ducts Biopsy Blood Cancer CD45 antigen Diagnosis Endoscopy Ethylenediaminetetraacetic acid Medical examination Medical prognosis Metastasis Observational studies Pancreatic cancer Patients Sampling Tumor cells Tumors |
| title | Liquid Biopsy for Detection of Pancreaticobiliary Cancers by Functional Enrichment and Immunofluorescent Profiling of Circulating Tumor Cells and Their Clusters |
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