Preparation of pH-sensitive carboxymethyl chitosan nanoparticles loaded with ginsenoside Rb1 and evaluation of drug release in vitro
Oral absorption of ginsenoside Rb1 (Rb1) is often hindered by the gastrointestinal tract. Carboxymethyl chitosan deoxycholic acid loaded with ginsenoside Rb1 nanoparticles (CMDA@Rb1-NPs), were prepared as a delivery system using a self-assembly technique with amphipathic deoxycholic acid grafted car...
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| Veröffentlicht in: | International journal of biological macromolecules 2024-05, Vol.267 (Pt 2), p.131487-131487, Article 131487 |
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| container_issue | Pt 2 |
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| container_title | International journal of biological macromolecules |
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| creator | An, Ziyuan Dong, Yujia Wang, Wanying Wang, Jiani Wu, Zhansheng Wang, Wenfei He, Yanhui Bao, Guoqiang |
| description | Oral absorption of ginsenoside Rb1 (Rb1) is often hindered by the gastrointestinal tract. Carboxymethyl chitosan deoxycholic acid loaded with ginsenoside Rb1 nanoparticles (CMDA@Rb1-NPs), were prepared as a delivery system using a self-assembly technique with amphipathic deoxycholic acid grafted carboxymethyl chitosan as the carrier, which improved the stability and embedding rate of Rb1. In addition, the CMDA@Rb1-NPs was encapsulated with sodium alginate by ion crosslinking method with additional layer (CMDAlg@Rb1-NPs). Scanning electron microscopy showed that the nanoparticles were spherical, evenly distributed, smooth and without obvious adhesion. By evaluating drug loading, entrapment efficiency, the encapsulation efficiency of Rb1 increased from 60.07 % to 72.14 % after grafting deoxycholic acid improvement and optimization. In vitro release results showed that the cumulative release of Rb1 by CMDAlg-NPs showed a pH dependent effect, which was |
| doi_str_mv | 10.1016/j.ijbiomac.2024.131487 |
| format | Article |
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Carboxymethyl chitosan deoxycholic acid loaded with ginsenoside Rb1 nanoparticles (CMDA@Rb1-NPs), were prepared as a delivery system using a self-assembly technique with amphipathic deoxycholic acid grafted carboxymethyl chitosan as the carrier, which improved the stability and embedding rate of Rb1. In addition, the CMDA@Rb1-NPs was encapsulated with sodium alginate by ion crosslinking method with additional layer (CMDAlg@Rb1-NPs). Scanning electron microscopy showed that the nanoparticles were spherical, evenly distributed, smooth and without obvious adhesion. By evaluating drug loading, entrapment efficiency, the encapsulation efficiency of Rb1 increased from 60.07 % to 72.14 % after grafting deoxycholic acid improvement and optimization. In vitro release results showed that the cumulative release of Rb1 by CMDAlg-NPs showed a pH dependent effect, which was <10 % in simulated gastric juice with pH 1.2, completely released with pH 7.4 for about 48 h. In addition, Rb1 and CMDAlg@Rb1-NPs had inhibitory effects on A549 cells, and the inhibitory effect of CMDAlg@Rb1-NPs was better. Therefore, all results indicated that CMDA/Alg@Rb1 nanoparticles might be a novel drug delivery system to improve the stability and embedding rate of Rb1, and has the potential to be applied in oral pharmaceutical preparations.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2024.131487</identifier><identifier>PMID: 38599430</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>absorption ; adhesion ; Carboxymethyl chitosan ; Cell Line, Tumor ; chitosan ; Chitosan - analogs & derivatives ; Chitosan - chemistry ; crosslinking ; deoxycholic acid ; digestive tract ; Drug Carriers - chemistry ; drug delivery systems ; Drug Liberation ; drugs ; electron microscopy ; encapsulation ; gastric juice ; ginsenosides ; Ginsenosides - chemistry ; Ginsenosides - pharmacokinetics ; Ginsenosides - pharmacology ; Humans ; Hydrogen-Ion Concentration ; nanoparticles ; Nanoparticles - chemistry ; Particle Size ; pH sensitivity ; Release mechanism ; sodium alginate ; surfactants</subject><ispartof>International journal of biological macromolecules, 2024-05, Vol.267 (Pt 2), p.131487-131487, Article 131487</ispartof><rights>2024 Elsevier B.V.</rights><rights>Copyright © 2024 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c348t-ac568c93b75cf89192cd86d1f2916a395fdc51fc1cbcc0e975e8592e2709136f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijbiomac.2024.131487$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38599430$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>An, Ziyuan</creatorcontrib><creatorcontrib>Dong, Yujia</creatorcontrib><creatorcontrib>Wang, Wanying</creatorcontrib><creatorcontrib>Wang, Jiani</creatorcontrib><creatorcontrib>Wu, Zhansheng</creatorcontrib><creatorcontrib>Wang, Wenfei</creatorcontrib><creatorcontrib>He, Yanhui</creatorcontrib><creatorcontrib>Bao, Guoqiang</creatorcontrib><title>Preparation of pH-sensitive carboxymethyl chitosan nanoparticles loaded with ginsenoside Rb1 and evaluation of drug release in vitro</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Oral absorption of ginsenoside Rb1 (Rb1) is often hindered by the gastrointestinal tract. Carboxymethyl chitosan deoxycholic acid loaded with ginsenoside Rb1 nanoparticles (CMDA@Rb1-NPs), were prepared as a delivery system using a self-assembly technique with amphipathic deoxycholic acid grafted carboxymethyl chitosan as the carrier, which improved the stability and embedding rate of Rb1. In addition, the CMDA@Rb1-NPs was encapsulated with sodium alginate by ion crosslinking method with additional layer (CMDAlg@Rb1-NPs). Scanning electron microscopy showed that the nanoparticles were spherical, evenly distributed, smooth and without obvious adhesion. By evaluating drug loading, entrapment efficiency, the encapsulation efficiency of Rb1 increased from 60.07 % to 72.14 % after grafting deoxycholic acid improvement and optimization. In vitro release results showed that the cumulative release of Rb1 by CMDAlg-NPs showed a pH dependent effect, which was <10 % in simulated gastric juice with pH 1.2, completely released with pH 7.4 for about 48 h. In addition, Rb1 and CMDAlg@Rb1-NPs had inhibitory effects on A549 cells, and the inhibitory effect of CMDAlg@Rb1-NPs was better. Therefore, all results indicated that CMDA/Alg@Rb1 nanoparticles might be a novel drug delivery system to improve the stability and embedding rate of Rb1, and has the potential to be applied in oral pharmaceutical preparations.</description><subject>absorption</subject><subject>adhesion</subject><subject>Carboxymethyl chitosan</subject><subject>Cell Line, Tumor</subject><subject>chitosan</subject><subject>Chitosan - analogs & derivatives</subject><subject>Chitosan - chemistry</subject><subject>crosslinking</subject><subject>deoxycholic acid</subject><subject>digestive tract</subject><subject>Drug Carriers - chemistry</subject><subject>drug delivery systems</subject><subject>Drug Liberation</subject><subject>drugs</subject><subject>electron microscopy</subject><subject>encapsulation</subject><subject>gastric juice</subject><subject>ginsenosides</subject><subject>Ginsenosides - chemistry</subject><subject>Ginsenosides - pharmacokinetics</subject><subject>Ginsenosides - pharmacology</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Particle Size</subject><subject>pH sensitivity</subject><subject>Release mechanism</subject><subject>sodium alginate</subject><subject>surfactants</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vFDEMhiMEokvhL1Q5cpklnsxHcgNVQCtVAiE4RxnH081qNlmSzJa988M71ba99mTJel5b9sPYBYg1COg-bdd-O_i4s7iuRd2sQUKj-ldsBarXlRBCvmYrAQ1UCqQ4Y-9y3i7drgX1lp1J1WrdSLFi_38m2ttki4-Bx5Hvr6pMIfviD8TRpiH-O-6obI4Tx40vMdvAgw1xyRSPE2U-RevI8TtfNvzWhyUds3fEfw3AbXCcDnaan-e7NN_yRBPZTNwHfvAlxffszWinTB8e6zn78-3r78ur6ubH9-vLLzcVykaVymLbKdRy6FsclQZdo1Odg7HW0Fmp29FhCyMCDoiCdN_ScmdNdS80yG6U5-zjae4-xb8z5WJ2PiNNkw0U52wktLKXou7ly6hYSK16aBa0O6GYYs6JRrNPfmfT0YAwD7LM1jzJMg-yzEnWErx43DEPO3LPsSc7C_D5BNDylIOnZDJ6CkjOJ8JiXPQv7bgHa4-rQA</recordid><startdate>20240501</startdate><enddate>20240501</enddate><creator>An, Ziyuan</creator><creator>Dong, Yujia</creator><creator>Wang, Wanying</creator><creator>Wang, Jiani</creator><creator>Wu, Zhansheng</creator><creator>Wang, Wenfei</creator><creator>He, Yanhui</creator><creator>Bao, Guoqiang</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240501</creationdate><title>Preparation of pH-sensitive carboxymethyl chitosan nanoparticles loaded with ginsenoside Rb1 and evaluation of drug release in vitro</title><author>An, Ziyuan ; Dong, Yujia ; Wang, Wanying ; Wang, Jiani ; Wu, Zhansheng ; Wang, Wenfei ; He, Yanhui ; Bao, Guoqiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-ac568c93b75cf89192cd86d1f2916a395fdc51fc1cbcc0e975e8592e2709136f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>absorption</topic><topic>adhesion</topic><topic>Carboxymethyl chitosan</topic><topic>Cell Line, Tumor</topic><topic>chitosan</topic><topic>Chitosan - analogs & derivatives</topic><topic>Chitosan - chemistry</topic><topic>crosslinking</topic><topic>deoxycholic acid</topic><topic>digestive tract</topic><topic>Drug Carriers - chemistry</topic><topic>drug delivery systems</topic><topic>Drug Liberation</topic><topic>drugs</topic><topic>electron microscopy</topic><topic>encapsulation</topic><topic>gastric juice</topic><topic>ginsenosides</topic><topic>Ginsenosides - chemistry</topic><topic>Ginsenosides - pharmacokinetics</topic><topic>Ginsenosides - pharmacology</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Particle Size</topic><topic>pH sensitivity</topic><topic>Release mechanism</topic><topic>sodium alginate</topic><topic>surfactants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>An, Ziyuan</creatorcontrib><creatorcontrib>Dong, Yujia</creatorcontrib><creatorcontrib>Wang, Wanying</creatorcontrib><creatorcontrib>Wang, Jiani</creatorcontrib><creatorcontrib>Wu, Zhansheng</creatorcontrib><creatorcontrib>Wang, Wenfei</creatorcontrib><creatorcontrib>He, Yanhui</creatorcontrib><creatorcontrib>Bao, Guoqiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>An, Ziyuan</au><au>Dong, Yujia</au><au>Wang, Wanying</au><au>Wang, Jiani</au><au>Wu, Zhansheng</au><au>Wang, Wenfei</au><au>He, Yanhui</au><au>Bao, Guoqiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of pH-sensitive carboxymethyl chitosan nanoparticles loaded with ginsenoside Rb1 and evaluation of drug release in vitro</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2024-05-01</date><risdate>2024</risdate><volume>267</volume><issue>Pt 2</issue><spage>131487</spage><epage>131487</epage><pages>131487-131487</pages><artnum>131487</artnum><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>Oral absorption of ginsenoside Rb1 (Rb1) is often hindered by the gastrointestinal tract. Carboxymethyl chitosan deoxycholic acid loaded with ginsenoside Rb1 nanoparticles (CMDA@Rb1-NPs), were prepared as a delivery system using a self-assembly technique with amphipathic deoxycholic acid grafted carboxymethyl chitosan as the carrier, which improved the stability and embedding rate of Rb1. In addition, the CMDA@Rb1-NPs was encapsulated with sodium alginate by ion crosslinking method with additional layer (CMDAlg@Rb1-NPs). Scanning electron microscopy showed that the nanoparticles were spherical, evenly distributed, smooth and without obvious adhesion. By evaluating drug loading, entrapment efficiency, the encapsulation efficiency of Rb1 increased from 60.07 % to 72.14 % after grafting deoxycholic acid improvement and optimization. In vitro release results showed that the cumulative release of Rb1 by CMDAlg-NPs showed a pH dependent effect, which was <10 % in simulated gastric juice with pH 1.2, completely released with pH 7.4 for about 48 h. In addition, Rb1 and CMDAlg@Rb1-NPs had inhibitory effects on A549 cells, and the inhibitory effect of CMDAlg@Rb1-NPs was better. Therefore, all results indicated that CMDA/Alg@Rb1 nanoparticles might be a novel drug delivery system to improve the stability and embedding rate of Rb1, and has the potential to be applied in oral pharmaceutical preparations.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>38599430</pmid><doi>10.1016/j.ijbiomac.2024.131487</doi><tpages>1</tpages></addata></record> |
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| subjects | absorption adhesion Carboxymethyl chitosan Cell Line, Tumor chitosan Chitosan - analogs & derivatives Chitosan - chemistry crosslinking deoxycholic acid digestive tract Drug Carriers - chemistry drug delivery systems Drug Liberation drugs electron microscopy encapsulation gastric juice ginsenosides Ginsenosides - chemistry Ginsenosides - pharmacokinetics Ginsenosides - pharmacology Humans Hydrogen-Ion Concentration nanoparticles Nanoparticles - chemistry Particle Size pH sensitivity Release mechanism sodium alginate surfactants |
| title | Preparation of pH-sensitive carboxymethyl chitosan nanoparticles loaded with ginsenoside Rb1 and evaluation of drug release in vitro |
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