Rutin alleviates Sjogren’s syndrome via CaR/NLRP3/NF-κB signal pathway
Sjogren’s syndrome (SS) is an autoimmune disease. Its mechanism and treatment methods are unclear. The purpose of this study was to investigate the effects of rutin (Ru) on SS. Proteomics was used to detect differential proteins in the submandibular glands of normal mice and SS mice. Salivary secret...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Animal 2024-04, Vol.60 (4), p.411-419 |
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| description | Sjogren’s syndrome (SS) is an autoimmune disease. Its mechanism and treatment methods are unclear. The purpose of this study was to investigate the effects of rutin (Ru) on SS. Proteomics was used to detect differential proteins in the submandibular glands of normal mice and SS mice. Salivary secretion (SAS) and salivary gland index (SGI) were detected. Oxidative stress and inflammatory cytokine in submandibular glands were detected. The levels of NLRP3, ASC, Caspase-1, IL-1β, and p-NF-κBp65 in submandibular gland tissues and submandibular gland cells of overexpressed calcium-sensing receptor (over-CaR) mice and overexpressed CaR primary submandibular gland cells (over-CaR-PSGs) were detected. In total, 327 differential proteins were identified in the submandibular gland tissues of SS mice compared to control mice. CaR was one of the most differential proteins and significantly increased compared to control mice. Ru could significantly increase SGI and SGI, and inhibit oxidative stress and inflammatory cytokine in submandibular glands. In addition, Ru was shown to further improve SS via regulation of the CaR/NOD-like receptor thermal protein domain associated protein 3 (NLRP3)/nuclear factor kappa-B (NF-κB) signal pathway. Overexpression of CaR counteracted partial activity of Ru. CaR may be an important target for the treatment of SS. In addition, Ru improved the SS via the CaR/NLRP3/NF-κB signal pathway. This study provides a basis for the treatments for SS. |
| doi_str_mv | 10.1007/s11626-024-00893-4 |
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Its mechanism and treatment methods are unclear. The purpose of this study was to investigate the effects of rutin (Ru) on SS. Proteomics was used to detect differential proteins in the submandibular glands of normal mice and SS mice. Salivary secretion (SAS) and salivary gland index (SGI) were detected. Oxidative stress and inflammatory cytokine in submandibular glands were detected. The levels of NLRP3, ASC, Caspase-1, IL-1β, and p-NF-κBp65 in submandibular gland tissues and submandibular gland cells of overexpressed calcium-sensing receptor (over-CaR) mice and overexpressed CaR primary submandibular gland cells (over-CaR-PSGs) were detected. In total, 327 differential proteins were identified in the submandibular gland tissues of SS mice compared to control mice. CaR was one of the most differential proteins and significantly increased compared to control mice. Ru could significantly increase SGI and SGI, and inhibit oxidative stress and inflammatory cytokine in submandibular glands. In addition, Ru was shown to further improve SS via regulation of the CaR/NOD-like receptor thermal protein domain associated protein 3 (NLRP3)/nuclear factor kappa-B (NF-κB) signal pathway. Overexpression of CaR counteracted partial activity of Ru. CaR may be an important target for the treatment of SS. In addition, Ru improved the SS via the CaR/NLRP3/NF-κB signal pathway. This study provides a basis for the treatments for SS.</description><identifier>ISSN: 1071-2690</identifier><identifier>EISSN: 1543-706X</identifier><identifier>DOI: 10.1007/s11626-024-00893-4</identifier><identifier>PMID: 38587579</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animal Genetics and Genomics ; Autoimmune diseases ; Biomedical and Life Sciences ; calcium receptors ; Caspase-1 ; Cell Biology ; Cell Culture ; Cytokines ; Developmental Biology ; Exocrine glands ; Flavonoids ; Health services ; Immunotherapy ; Inflammation ; Life Sciences ; NF-κB protein ; Oxidative stress ; Pathogenesis ; protein domains ; Proteins ; Proteomics ; Receptors ; Rutin ; Salivary gland ; Salivary glands ; secretion ; signal transduction ; Sjogren's syndrome ; Stem Cells ; Submandibular gland ; Transcription factors</subject><ispartof>In vitro cellular & developmental biology. Animal, 2024-04, Vol.60 (4), p.411-419</ispartof><rights>The Society for In Vitro Biology 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Society for In Vitro Biology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c359t-b0bfab12708276a3265b5ad3947b2a92b65cf10adc3efcb9f3c7479ccca946dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11626-024-00893-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11626-024-00893-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27915,27916,41479,42548,51310</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38587579$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Jing</creatorcontrib><creatorcontrib>Xu, Meimei</creatorcontrib><creatorcontrib>Wu, Suling</creatorcontrib><title>Rutin alleviates Sjogren’s syndrome via CaR/NLRP3/NF-κB signal pathway</title><title>In vitro cellular & developmental biology. Animal</title><addtitle>In Vitro Cell.Dev.Biol.-Animal</addtitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><description>Sjogren’s syndrome (SS) is an autoimmune disease. Its mechanism and treatment methods are unclear. The purpose of this study was to investigate the effects of rutin (Ru) on SS. Proteomics was used to detect differential proteins in the submandibular glands of normal mice and SS mice. Salivary secretion (SAS) and salivary gland index (SGI) were detected. Oxidative stress and inflammatory cytokine in submandibular glands were detected. The levels of NLRP3, ASC, Caspase-1, IL-1β, and p-NF-κBp65 in submandibular gland tissues and submandibular gland cells of overexpressed calcium-sensing receptor (over-CaR) mice and overexpressed CaR primary submandibular gland cells (over-CaR-PSGs) were detected. In total, 327 differential proteins were identified in the submandibular gland tissues of SS mice compared to control mice. CaR was one of the most differential proteins and significantly increased compared to control mice. Ru could significantly increase SGI and SGI, and inhibit oxidative stress and inflammatory cytokine in submandibular glands. In addition, Ru was shown to further improve SS via regulation of the CaR/NOD-like receptor thermal protein domain associated protein 3 (NLRP3)/nuclear factor kappa-B (NF-κB) signal pathway. Overexpression of CaR counteracted partial activity of Ru. CaR may be an important target for the treatment of SS. In addition, Ru improved the SS via the CaR/NLRP3/NF-κB signal pathway. This study provides a basis for the treatments for SS.</description><subject>Animal Genetics and Genomics</subject><subject>Autoimmune diseases</subject><subject>Biomedical and Life Sciences</subject><subject>calcium receptors</subject><subject>Caspase-1</subject><subject>Cell Biology</subject><subject>Cell Culture</subject><subject>Cytokines</subject><subject>Developmental Biology</subject><subject>Exocrine glands</subject><subject>Flavonoids</subject><subject>Health services</subject><subject>Immunotherapy</subject><subject>Inflammation</subject><subject>Life Sciences</subject><subject>NF-κB protein</subject><subject>Oxidative stress</subject><subject>Pathogenesis</subject><subject>protein domains</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Receptors</subject><subject>Rutin</subject><subject>Salivary gland</subject><subject>Salivary glands</subject><subject>secretion</subject><subject>signal transduction</subject><subject>Sjogren's syndrome</subject><subject>Stem Cells</subject><subject>Submandibular gland</subject><subject>Transcription factors</subject><issn>1071-2690</issn><issn>1543-706X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkc9u1DAQh60K1JbSF-gBReLCxezY4z_xEVaUVloVtIDEzXIcZ5tVNlnsBLQ3XoNX6UPwEDwJLtuCxAF8GUvzzW80-gg5Y_CcAehZYkxxRYELClAapOKAHDMpkGpQHx_kP2hGuTJwRB6ltIb8DFOH5AhLWWqpzTG5XE5j2xeu68Ln1o0hFe_WwyqG_sfXb6lIu76OwyYUuVfM3XJ2tVi-xdnVOf1-87JI7ap3XbF14_UXt3tMHjauS-H0rp6QD-ev3s8v6OLN68v5iwX1KM1IK6gaVzGuoeRaOeRKVtLVaISuuDO8UtI3DFztMTS-Mg16LbTx3jsjVF3jCXm2z93G4dMU0mg3bfKh61wfhilZZBKVLjni_1FAobVCARl9-he6HqaYz7ulJJOMAeeZ4nvKxyGlGBq7je3GxZ1lYG-d2L0Tm53YX06syENP7qKnahPq3yP3EjKAeyDlVr8K8c_uf8T-BDw8ls8</recordid><startdate>20240401</startdate><enddate>20240401</enddate><creator>He, Jing</creator><creator>Xu, Meimei</creator><creator>Wu, Suling</creator><general>Springer US</general><general>Society for In Vitro Biology</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>4T-</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240401</creationdate><title>Rutin alleviates Sjogren’s syndrome via CaR/NLRP3/NF-κB signal pathway</title><author>He, Jing ; Xu, Meimei ; Wu, Suling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-b0bfab12708276a3265b5ad3947b2a92b65cf10adc3efcb9f3c7479ccca946dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animal Genetics and Genomics</topic><topic>Autoimmune diseases</topic><topic>Biomedical and Life Sciences</topic><topic>calcium receptors</topic><topic>Caspase-1</topic><topic>Cell Biology</topic><topic>Cell Culture</topic><topic>Cytokines</topic><topic>Developmental Biology</topic><topic>Exocrine glands</topic><topic>Flavonoids</topic><topic>Health services</topic><topic>Immunotherapy</topic><topic>Inflammation</topic><topic>Life Sciences</topic><topic>NF-κB protein</topic><topic>Oxidative stress</topic><topic>Pathogenesis</topic><topic>protein domains</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Receptors</topic><topic>Rutin</topic><topic>Salivary gland</topic><topic>Salivary glands</topic><topic>secretion</topic><topic>signal transduction</topic><topic>Sjogren's syndrome</topic><topic>Stem Cells</topic><topic>Submandibular gland</topic><topic>Transcription factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Jing</creatorcontrib><creatorcontrib>Xu, Meimei</creatorcontrib><creatorcontrib>Wu, Suling</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Docstoc</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>In vitro cellular & developmental biology. Animal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Jing</au><au>Xu, Meimei</au><au>Wu, Suling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rutin alleviates Sjogren’s syndrome via CaR/NLRP3/NF-κB signal pathway</atitle><jtitle>In vitro cellular & developmental biology. Animal</jtitle><stitle>In Vitro Cell.Dev.Biol.-Animal</stitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><date>2024-04-01</date><risdate>2024</risdate><volume>60</volume><issue>4</issue><spage>411</spage><epage>419</epage><pages>411-419</pages><issn>1071-2690</issn><eissn>1543-706X</eissn><abstract>Sjogren’s syndrome (SS) is an autoimmune disease. Its mechanism and treatment methods are unclear. The purpose of this study was to investigate the effects of rutin (Ru) on SS. Proteomics was used to detect differential proteins in the submandibular glands of normal mice and SS mice. Salivary secretion (SAS) and salivary gland index (SGI) were detected. Oxidative stress and inflammatory cytokine in submandibular glands were detected. The levels of NLRP3, ASC, Caspase-1, IL-1β, and p-NF-κBp65 in submandibular gland tissues and submandibular gland cells of overexpressed calcium-sensing receptor (over-CaR) mice and overexpressed CaR primary submandibular gland cells (over-CaR-PSGs) were detected. In total, 327 differential proteins were identified in the submandibular gland tissues of SS mice compared to control mice. CaR was one of the most differential proteins and significantly increased compared to control mice. Ru could significantly increase SGI and SGI, and inhibit oxidative stress and inflammatory cytokine in submandibular glands. In addition, Ru was shown to further improve SS via regulation of the CaR/NOD-like receptor thermal protein domain associated protein 3 (NLRP3)/nuclear factor kappa-B (NF-κB) signal pathway. Overexpression of CaR counteracted partial activity of Ru. CaR may be an important target for the treatment of SS. In addition, Ru improved the SS via the CaR/NLRP3/NF-κB signal pathway. This study provides a basis for the treatments for SS.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>38587579</pmid><doi>10.1007/s11626-024-00893-4</doi><tpages>9</tpages></addata></record> |
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| subjects | Animal Genetics and Genomics Autoimmune diseases Biomedical and Life Sciences calcium receptors Caspase-1 Cell Biology Cell Culture Cytokines Developmental Biology Exocrine glands Flavonoids Health services Immunotherapy Inflammation Life Sciences NF-κB protein Oxidative stress Pathogenesis protein domains Proteins Proteomics Receptors Rutin Salivary gland Salivary glands secretion signal transduction Sjogren's syndrome Stem Cells Submandibular gland Transcription factors |
| title | Rutin alleviates Sjogren’s syndrome via CaR/NLRP3/NF-κB signal pathway |
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