Exosomes from SHED-MSC regulate polarization and stress oxidative indexes in THP-1 derived M1 macrophages
The inhibition of M1 macrophages may be interesting for targeted therapy with mesenchymal stem cell-derived Exosomes (MSC-EXOs). This study aimed to investigate the stem cells of human exfoliated deciduous teeth-derived EXOs (SHED-MSC-EXOs) effect on regulating the pro- and anti-oxidant indexes and...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 2024-05, Vol.755, p.109987-109987, Article 109987 |
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| creator | Fallah, Ali Hosseinzadeh Colagar, Abasalt Khosravi, Ayyoob Saeidi, Mohsen |
| description | The inhibition of M1 macrophages may be interesting for targeted therapy with mesenchymal stem cell-derived Exosomes (MSC-EXOs). This study aimed to investigate the stem cells of human exfoliated deciduous teeth-derived EXOs (SHED-MSC-EXOs) effect on regulating the pro- and anti-oxidant indexes and inhibiting M1 macrophage polarization. Besides, an in-silico analysis of SHED-MSC-EXO miRNAs as the highest frequency of small RNAs in the exosomes was performed to discover the possible mechanism.
The flow cytometry analysis of CD80 and CD86 as M1-specific markers confirmed the polarization of macrophages derived from THP-1 cells. After exosome isolation, characterization, and internalization, THP-1-derived M1 macrophages were treated with SHED-MSC-EXOs. M1-specific markers and pro- and anti-oxidant indexes were evaluated. For in-silico analysis of SHED-MSC-EXOs miRNAs, initial miRNA array data of SHED-EXOs is collected from GEO, and the interaction of the miRNAs in M1 macrophage polarization (M1P), mitochondrial oxidative stress (MOS) and LPS-induced oxidative stress (LOS) were analyzed by miRWalk 3.0 server. Outcomes were filtered by 75th percentile signal intensity, score cut-off ≥0.95, minimum free energy (MEF)≤ −20 kcal/mol, and seed = 1.
It shows a decrease in the expression of CD80 and CD81, a reduction in pro-oxidant indicators, and an increase in the anti-oxidant indexes (P |
| doi_str_mv | 10.1016/j.abb.2024.109987 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3034248632</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0003986124001061</els_id><sourcerecordid>3153744194</sourcerecordid><originalsourceid>FETCH-LOGICAL-c338t-c1d8ad669a4602afb28d35afc16563001a5173aa01fe17ae0f5befa920f63a5e3</originalsourceid><addsrcrecordid>eNqFkU9v1DAQxS0EarelH4AL8pFLFk_sOIk4oe3SRWoFUsvZmsTj4lUSL3a2WvrpcbWFYznNH_3mSW8eY-9ALEGA_rhdYtctS1GqPLdtU79ii9zoQshGvWYLIYQs2kbDKTtLaSsEgNLlCTuVTVW3baUXzK8PIYWREncxjPx2s74sbm5XPNL9fsCZ-C4MGP0jzj5MHCfL0xwpJR4O3ublA3E_WTpkAT_xu833ArilmPeW3wAfsY9h9xPvKb1lbxwOiS6e6zn78WV9t9oU19-uvq4-Xxe9lM1c9GAbtFq3qLQo0XVlY2WFrgddaZktYAW1RBTgCGok4aqOHLalcFpiRfKcfTjq7mL4tac0m9GnnoYBJwr7ZCRUslYKWvV_VEhVqkbLMqNwRLOflCI5s4t-xPjbgDBPYZityWGYpzDMMYx88_5Zft-NZP9d_P1-Bj4dAcr_ePAUTeo9TT1ZH6mfjQ3-Bfk_vK-ZTg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3034248632</pqid></control><display><type>article</type><title>Exosomes from SHED-MSC regulate polarization and stress oxidative indexes in THP-1 derived M1 macrophages</title><source>Elsevier ScienceDirect Journals</source><creator>Fallah, Ali ; Hosseinzadeh Colagar, Abasalt ; Khosravi, Ayyoob ; Saeidi, Mohsen</creator><creatorcontrib>Fallah, Ali ; Hosseinzadeh Colagar, Abasalt ; Khosravi, Ayyoob ; Saeidi, Mohsen</creatorcontrib><description>The inhibition of M1 macrophages may be interesting for targeted therapy with mesenchymal stem cell-derived Exosomes (MSC-EXOs). This study aimed to investigate the stem cells of human exfoliated deciduous teeth-derived EXOs (SHED-MSC-EXOs) effect on regulating the pro- and anti-oxidant indexes and inhibiting M1 macrophage polarization. Besides, an in-silico analysis of SHED-MSC-EXO miRNAs as the highest frequency of small RNAs in the exosomes was performed to discover the possible mechanism.
The flow cytometry analysis of CD80 and CD86 as M1-specific markers confirmed the polarization of macrophages derived from THP-1 cells. After exosome isolation, characterization, and internalization, THP-1-derived M1 macrophages were treated with SHED-MSC-EXOs. M1-specific markers and pro- and anti-oxidant indexes were evaluated. For in-silico analysis of SHED-MSC-EXOs miRNAs, initial miRNA array data of SHED-EXOs is collected from GEO, and the interaction of the miRNAs in M1 macrophage polarization (M1P), mitochondrial oxidative stress (MOS) and LPS-induced oxidative stress (LOS) were analyzed by miRWalk 3.0 server. Outcomes were filtered by 75th percentile signal intensity, score cut-off ≥0.95, minimum free energy (MEF)≤ −20 kcal/mol, and seed = 1.
It shows a decrease in the expression of CD80 and CD81, a reduction in pro-oxidant indicators, and an increase in the anti-oxidant indexes (P < 0.05). Computational analysis showed that eight microRNAs of SHED-MSC-EXO miRNAs can bind to and interfere with the expression of candidate genes in the M1P, MOS, and LOS pathways simultaneously.
SHED-MSCs-EXOs can be utilized to treat conditions related to M1 macrophage-induced diseases (M1IDs) due to their unique physical properties and ability to penetrate target cells easily.
[Display omitted]
•SHED-MSCs-EXOs regulate levels of pro-oxidant markers (NO, MDA) and enhance levels of anti-oxidant markers (TAC, CAT, SOD).•The SHED-MSCs-EXOs can target the polarization of M1 macrophages and leading to a reduction in CD80 and CD86 levels.•The exosomal-miRNAs from SHED bind to and interfere with the expression of potential genes in the M1P, MOS, and LOS pathways.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/j.abb.2024.109987</identifier><identifier>PMID: 38579956</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>antioxidants ; biophysics ; computer simulation ; Exosome ; exosomes ; flow cytometry ; Gibbs free energy ; humans ; LPS-Induced oxidative stress ; Macrophage polarization ; macrophages ; microRNA ; mitochondria ; Mitochondrial oxidative stress ; oxidative stress ; SHED-MSC-EXO miRNAs ; therapeutics</subject><ispartof>Archives of biochemistry and biophysics, 2024-05, Vol.755, p.109987-109987, Article 109987</ispartof><rights>2024 Elsevier Inc.</rights><rights>Copyright © 2024. Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c338t-c1d8ad669a4602afb28d35afc16563001a5173aa01fe17ae0f5befa920f63a5e3</cites><orcidid>0000-0001-6536-8250 ; 0000-0002-2258-3070</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0003986124001061$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38579956$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fallah, Ali</creatorcontrib><creatorcontrib>Hosseinzadeh Colagar, Abasalt</creatorcontrib><creatorcontrib>Khosravi, Ayyoob</creatorcontrib><creatorcontrib>Saeidi, Mohsen</creatorcontrib><title>Exosomes from SHED-MSC regulate polarization and stress oxidative indexes in THP-1 derived M1 macrophages</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>The inhibition of M1 macrophages may be interesting for targeted therapy with mesenchymal stem cell-derived Exosomes (MSC-EXOs). This study aimed to investigate the stem cells of human exfoliated deciduous teeth-derived EXOs (SHED-MSC-EXOs) effect on regulating the pro- and anti-oxidant indexes and inhibiting M1 macrophage polarization. Besides, an in-silico analysis of SHED-MSC-EXO miRNAs as the highest frequency of small RNAs in the exosomes was performed to discover the possible mechanism.
The flow cytometry analysis of CD80 and CD86 as M1-specific markers confirmed the polarization of macrophages derived from THP-1 cells. After exosome isolation, characterization, and internalization, THP-1-derived M1 macrophages were treated with SHED-MSC-EXOs. M1-specific markers and pro- and anti-oxidant indexes were evaluated. For in-silico analysis of SHED-MSC-EXOs miRNAs, initial miRNA array data of SHED-EXOs is collected from GEO, and the interaction of the miRNAs in M1 macrophage polarization (M1P), mitochondrial oxidative stress (MOS) and LPS-induced oxidative stress (LOS) were analyzed by miRWalk 3.0 server. Outcomes were filtered by 75th percentile signal intensity, score cut-off ≥0.95, minimum free energy (MEF)≤ −20 kcal/mol, and seed = 1.
It shows a decrease in the expression of CD80 and CD81, a reduction in pro-oxidant indicators, and an increase in the anti-oxidant indexes (P < 0.05). Computational analysis showed that eight microRNAs of SHED-MSC-EXO miRNAs can bind to and interfere with the expression of candidate genes in the M1P, MOS, and LOS pathways simultaneously.
SHED-MSCs-EXOs can be utilized to treat conditions related to M1 macrophage-induced diseases (M1IDs) due to their unique physical properties and ability to penetrate target cells easily.
[Display omitted]
•SHED-MSCs-EXOs regulate levels of pro-oxidant markers (NO, MDA) and enhance levels of anti-oxidant markers (TAC, CAT, SOD).•The SHED-MSCs-EXOs can target the polarization of M1 macrophages and leading to a reduction in CD80 and CD86 levels.•The exosomal-miRNAs from SHED bind to and interfere with the expression of potential genes in the M1P, MOS, and LOS pathways.</description><subject>antioxidants</subject><subject>biophysics</subject><subject>computer simulation</subject><subject>Exosome</subject><subject>exosomes</subject><subject>flow cytometry</subject><subject>Gibbs free energy</subject><subject>humans</subject><subject>LPS-Induced oxidative stress</subject><subject>Macrophage polarization</subject><subject>macrophages</subject><subject>microRNA</subject><subject>mitochondria</subject><subject>Mitochondrial oxidative stress</subject><subject>oxidative stress</subject><subject>SHED-MSC-EXO miRNAs</subject><subject>therapeutics</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkU9v1DAQxS0EarelH4AL8pFLFk_sOIk4oe3SRWoFUsvZmsTj4lUSL3a2WvrpcbWFYznNH_3mSW8eY-9ALEGA_rhdYtctS1GqPLdtU79ii9zoQshGvWYLIYQs2kbDKTtLaSsEgNLlCTuVTVW3baUXzK8PIYWREncxjPx2s74sbm5XPNL9fsCZ-C4MGP0jzj5MHCfL0xwpJR4O3ublA3E_WTpkAT_xu833ArilmPeW3wAfsY9h9xPvKb1lbxwOiS6e6zn78WV9t9oU19-uvq4-Xxe9lM1c9GAbtFq3qLQo0XVlY2WFrgddaZktYAW1RBTgCGok4aqOHLalcFpiRfKcfTjq7mL4tac0m9GnnoYBJwr7ZCRUslYKWvV_VEhVqkbLMqNwRLOflCI5s4t-xPjbgDBPYZityWGYpzDMMYx88_5Zft-NZP9d_P1-Bj4dAcr_ePAUTeo9TT1ZH6mfjQ3-Bfk_vK-ZTg</recordid><startdate>20240501</startdate><enddate>20240501</enddate><creator>Fallah, Ali</creator><creator>Hosseinzadeh Colagar, Abasalt</creator><creator>Khosravi, Ayyoob</creator><creator>Saeidi, Mohsen</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-6536-8250</orcidid><orcidid>https://orcid.org/0000-0002-2258-3070</orcidid></search><sort><creationdate>20240501</creationdate><title>Exosomes from SHED-MSC regulate polarization and stress oxidative indexes in THP-1 derived M1 macrophages</title><author>Fallah, Ali ; Hosseinzadeh Colagar, Abasalt ; Khosravi, Ayyoob ; Saeidi, Mohsen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-c1d8ad669a4602afb28d35afc16563001a5173aa01fe17ae0f5befa920f63a5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>antioxidants</topic><topic>biophysics</topic><topic>computer simulation</topic><topic>Exosome</topic><topic>exosomes</topic><topic>flow cytometry</topic><topic>Gibbs free energy</topic><topic>humans</topic><topic>LPS-Induced oxidative stress</topic><topic>Macrophage polarization</topic><topic>macrophages</topic><topic>microRNA</topic><topic>mitochondria</topic><topic>Mitochondrial oxidative stress</topic><topic>oxidative stress</topic><topic>SHED-MSC-EXO miRNAs</topic><topic>therapeutics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fallah, Ali</creatorcontrib><creatorcontrib>Hosseinzadeh Colagar, Abasalt</creatorcontrib><creatorcontrib>Khosravi, Ayyoob</creatorcontrib><creatorcontrib>Saeidi, Mohsen</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fallah, Ali</au><au>Hosseinzadeh Colagar, Abasalt</au><au>Khosravi, Ayyoob</au><au>Saeidi, Mohsen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exosomes from SHED-MSC regulate polarization and stress oxidative indexes in THP-1 derived M1 macrophages</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>2024-05-01</date><risdate>2024</risdate><volume>755</volume><spage>109987</spage><epage>109987</epage><pages>109987-109987</pages><artnum>109987</artnum><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>The inhibition of M1 macrophages may be interesting for targeted therapy with mesenchymal stem cell-derived Exosomes (MSC-EXOs). This study aimed to investigate the stem cells of human exfoliated deciduous teeth-derived EXOs (SHED-MSC-EXOs) effect on regulating the pro- and anti-oxidant indexes and inhibiting M1 macrophage polarization. Besides, an in-silico analysis of SHED-MSC-EXO miRNAs as the highest frequency of small RNAs in the exosomes was performed to discover the possible mechanism.
The flow cytometry analysis of CD80 and CD86 as M1-specific markers confirmed the polarization of macrophages derived from THP-1 cells. After exosome isolation, characterization, and internalization, THP-1-derived M1 macrophages were treated with SHED-MSC-EXOs. M1-specific markers and pro- and anti-oxidant indexes were evaluated. For in-silico analysis of SHED-MSC-EXOs miRNAs, initial miRNA array data of SHED-EXOs is collected from GEO, and the interaction of the miRNAs in M1 macrophage polarization (M1P), mitochondrial oxidative stress (MOS) and LPS-induced oxidative stress (LOS) were analyzed by miRWalk 3.0 server. Outcomes were filtered by 75th percentile signal intensity, score cut-off ≥0.95, minimum free energy (MEF)≤ −20 kcal/mol, and seed = 1.
It shows a decrease in the expression of CD80 and CD81, a reduction in pro-oxidant indicators, and an increase in the anti-oxidant indexes (P < 0.05). Computational analysis showed that eight microRNAs of SHED-MSC-EXO miRNAs can bind to and interfere with the expression of candidate genes in the M1P, MOS, and LOS pathways simultaneously.
SHED-MSCs-EXOs can be utilized to treat conditions related to M1 macrophage-induced diseases (M1IDs) due to their unique physical properties and ability to penetrate target cells easily.
[Display omitted]
•SHED-MSCs-EXOs regulate levels of pro-oxidant markers (NO, MDA) and enhance levels of anti-oxidant markers (TAC, CAT, SOD).•The SHED-MSCs-EXOs can target the polarization of M1 macrophages and leading to a reduction in CD80 and CD86 levels.•The exosomal-miRNAs from SHED bind to and interfere with the expression of potential genes in the M1P, MOS, and LOS pathways.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>38579956</pmid><doi>10.1016/j.abb.2024.109987</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-6536-8250</orcidid><orcidid>https://orcid.org/0000-0002-2258-3070</orcidid></addata></record> |
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| subjects | antioxidants biophysics computer simulation Exosome exosomes flow cytometry Gibbs free energy humans LPS-Induced oxidative stress Macrophage polarization macrophages microRNA mitochondria Mitochondrial oxidative stress oxidative stress SHED-MSC-EXO miRNAs therapeutics |
| title | Exosomes from SHED-MSC regulate polarization and stress oxidative indexes in THP-1 derived M1 macrophages |
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