Green and efficient method to acquire high-value phycobiliprotein from microalgal biomass involving deep eutectic solvent-based ultrasound-assisted extraction

Phycoerythrin (PE) is a phycobiliprotein holding great potential as a high-value food colorant and medicine. Deep eutectic solvent (DES)-based ultrasound-assisted extraction (UAE) was applied to extract B-PE by disrupting the resistant polysaccharide cell wall of Porphyridium purpureum. The solubili...

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Veröffentlicht in:Food chemistry 2024-08, Vol.449, p.139196-139196, Article 139196
Hauptverfasser: Li, Ke, Jiang, Chunxue, Han, Sang-Il, Kang, Seulgi, Chen, Jingyan, Won, Danbi, Kang, Yua, Bae, Boyeon, Choi, Yoon-E, Kim, Hyung Sik, Lee, Jeongmi
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container_end_page 139196
container_issue
container_start_page 139196
container_title Food chemistry
container_volume 449
creator Li, Ke
Jiang, Chunxue
Han, Sang-Il
Kang, Seulgi
Chen, Jingyan
Won, Danbi
Kang, Yua
Bae, Boyeon
Choi, Yoon-E
Kim, Hyung Sik
Lee, Jeongmi
description Phycoerythrin (PE) is a phycobiliprotein holding great potential as a high-value food colorant and medicine. Deep eutectic solvent (DES)-based ultrasound-assisted extraction (UAE) was applied to extract B-PE by disrupting the resistant polysaccharide cell wall of Porphyridium purpureum. The solubility of cell wall monomers in 31 DESs was predicted using COSMO-RS. Five glycerol-based DESs were tested for extraction, all of which showed significantly higher B-PE yields by up to 13.5 folds than water. The DES-dependent B-PE extraction efficiencies were proposedly associated with different cell disrupting capabilities and protein stabilizing effects of DESs. The DES-based UAE method could be considered green according to a metric assessment tool, AGREEprep. The crude extract containing DES was further subjected to aqueous two-phase system, two-step ammonium sulfate precipitation, and ultrafiltration processes. The final purified B-PE had a PE purity ratio of 3.60 and a PC purity ratio of 0.08, comparable to the purity of commercial products. •COSMO-RS predicted the solubility of microalgal cell wall components in DESs.•High solubility-predicted DESs could disrupt the resistant cell wall effectively.•PGly-based UAE of cells could produce the B-PE extract the most efficiently.•PGly-based UAE method was considered green based on AGREEprep evaluation.•High-purity B-PE was obtained from DES extract by ATPS, followed by ASP and UF.
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The final purified B-PE had a PE purity ratio of 3.60 and a PC purity ratio of 0.08, comparable to the purity of commercial products. •COSMO-RS predicted the solubility of microalgal cell wall components in DESs.•High solubility-predicted DESs could disrupt the resistant cell wall effectively.•PGly-based UAE of cells could produce the B-PE extract the most efficiently.•PGly-based UAE method was considered green based on AGREEprep evaluation.•High-purity B-PE was obtained from DES extract by ATPS, followed by ASP and UF.</description><subject>Cell disruption</subject><subject>Deep eutectic solvents</subject><subject>Extraction</subject><subject>Microalgae</subject><subject>Phycoerythrin</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkc1uFDEQhC0EIpvAK0Q-cpnFPxPbcwNFEJAicYGz5bHbO17N2BvbsyIvk2eNV5tw5dRSqapLpQ-ha0q2lFDxeb_1KTk7wbJlhPVbygc6iDdoQ5XknSSSvUUbwonqFO3FBbosZU8IYYSq9-iCqxtFpZIb9HSXASI20WHwPtgAseIF6pQcrgkb-7CGDHgKu6k7mnkFfJgebRrDHA45VQgR-5wWvASbk5l3ZsZjSIspBYd4TPMxxB12AAcMawVbg8Wlqa2lG00Bh9e5ZlPSGl3XQqHUpsHfpjVvih_QO2_mAh9f7hX68_3b79sf3f2vu5-3X-87y4WqHVWMMdEPxgzgiKdSSEc48x4GKZminAgu_CiUHAEUG5xn1JPRgR9urJXAr9Cn89826mGFUvUSioV5NhHSWjQnvGe95Ew2qzhb2-BSMnh9yGEx-VFTok9s9F6_stEnNvrMpgWvXzrWcQH3L_YKoxm-nA3Qlh4DZF1OPCy4hsBW7VL4X8cz0_6okw</recordid><startdate>20240815</startdate><enddate>20240815</enddate><creator>Li, Ke</creator><creator>Jiang, Chunxue</creator><creator>Han, Sang-Il</creator><creator>Kang, Seulgi</creator><creator>Chen, Jingyan</creator><creator>Won, Danbi</creator><creator>Kang, Yua</creator><creator>Bae, Boyeon</creator><creator>Choi, Yoon-E</creator><creator>Kim, Hyung Sik</creator><creator>Lee, Jeongmi</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20240815</creationdate><title>Green and efficient method to acquire high-value phycobiliprotein from microalgal biomass involving deep eutectic solvent-based ultrasound-assisted extraction</title><author>Li, Ke ; Jiang, Chunxue ; Han, Sang-Il ; Kang, Seulgi ; Chen, Jingyan ; Won, Danbi ; Kang, Yua ; Bae, Boyeon ; Choi, Yoon-E ; Kim, Hyung Sik ; Lee, Jeongmi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-18222649aa9ed0f1767d032ffe97728130636fb687bee829df21f0bdef95cc7e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Cell disruption</topic><topic>Deep eutectic solvents</topic><topic>Extraction</topic><topic>Microalgae</topic><topic>Phycoerythrin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Ke</creatorcontrib><creatorcontrib>Jiang, Chunxue</creatorcontrib><creatorcontrib>Han, Sang-Il</creatorcontrib><creatorcontrib>Kang, Seulgi</creatorcontrib><creatorcontrib>Chen, Jingyan</creatorcontrib><creatorcontrib>Won, Danbi</creatorcontrib><creatorcontrib>Kang, Yua</creatorcontrib><creatorcontrib>Bae, Boyeon</creatorcontrib><creatorcontrib>Choi, Yoon-E</creatorcontrib><creatorcontrib>Kim, Hyung Sik</creatorcontrib><creatorcontrib>Lee, Jeongmi</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Ke</au><au>Jiang, Chunxue</au><au>Han, Sang-Il</au><au>Kang, Seulgi</au><au>Chen, Jingyan</au><au>Won, Danbi</au><au>Kang, Yua</au><au>Bae, Boyeon</au><au>Choi, Yoon-E</au><au>Kim, Hyung Sik</au><au>Lee, Jeongmi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Green and efficient method to acquire high-value phycobiliprotein from microalgal biomass involving deep eutectic solvent-based ultrasound-assisted extraction</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2024-08-15</date><risdate>2024</risdate><volume>449</volume><spage>139196</spage><epage>139196</epage><pages>139196-139196</pages><artnum>139196</artnum><issn>0308-8146</issn><eissn>1873-7072</eissn><abstract>Phycoerythrin (PE) is a phycobiliprotein holding great potential as a high-value food colorant and medicine. 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The final purified B-PE had a PE purity ratio of 3.60 and a PC purity ratio of 0.08, comparable to the purity of commercial products. •COSMO-RS predicted the solubility of microalgal cell wall components in DESs.•High solubility-predicted DESs could disrupt the resistant cell wall effectively.•PGly-based UAE of cells could produce the B-PE extract the most efficiently.•PGly-based UAE method was considered green based on AGREEprep evaluation.•High-purity B-PE was obtained from DES extract by ATPS, followed by ASP and UF.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>38581787</pmid><doi>10.1016/j.foodchem.2024.139196</doi><tpages>1</tpages></addata></record>
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subjects Cell disruption
Deep eutectic solvents
Extraction
Microalgae
Phycoerythrin
title Green and efficient method to acquire high-value phycobiliprotein from microalgal biomass involving deep eutectic solvent-based ultrasound-assisted extraction
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