Possible alternative strategies to implement basophil activation testing in multicentric studies

The Basophil Activation Test (BAT) enables flow cytometry characterization of basophil reactivity against specific allergenic molecules. The focus now revolves around democratizing this tool, but, as blood sample stability could be challenging, after having developed a simplified approach, herein, w...

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Veröffentlicht in:Cytometry. Part B, Clinical cytometry Clinical cytometry, 2024-09, Vol.106 (5), p.392-404
Hauptverfasser: Bourgoin, Pénélope, Dupont, Thomas, Agabriel, Chantal, Carsin, Ania, Verles, Aurélie, Cabanski, Maciej, Vitaliti, Alessandra, Busnel, Jean‐Marc
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container_end_page 404
container_issue 5
container_start_page 392
container_title Cytometry. Part B, Clinical cytometry
container_volume 106
creator Bourgoin, Pénélope
Dupont, Thomas
Agabriel, Chantal
Carsin, Ania
Verles, Aurélie
Cabanski, Maciej
Vitaliti, Alessandra
Busnel, Jean‐Marc
description The Basophil Activation Test (BAT) enables flow cytometry characterization of basophil reactivity against specific allergenic molecules. The focus now revolves around democratizing this tool, but, as blood sample stability could be challenging, after having developed a simplified approach, herein, we aimed to characterize two strategies for implementing BAT in multicentric studies: store and ship blood before or after sample processing. Fresh heparin‐ and EDTA‐anticoagulated whole blood samples followed both BAT workflows: “collect, store, process & analyze” or “collect, process, store & analyze”. Storage temperatures of 18–25 °C or 2–8 °C and preservation times from 0 to 7 days were considered. Interleukin‐3 was also evaluated. With the “collect, store, process & analyze” workflow, heparin‐anticoagulated blood and 18–25 °C storage were better than other conditions. While remaining possible, basophil activation exhibited a possible reactivity decay after 24 h. Under the conditions tested, interleukin‐3 had no role in enhancing basophil reactivity after storage. Conversely, the “collect, process, store & analyze” workflow demonstrated that either heparin‐ or EDTA‐anticoagulated blood can be processed and kept up to 7 days at 18–25 °C or 2–8 °C before being analyzed. Various strategies can be implemented to integrate BAT in multicentric studies. The “collect, store, process & analyze” workflow remains a simplified logistical approach, but depending on time required to ship from the clinical centers to the reference laboratories, it might not be applicable, or should be used with caution. The “collect, process, store & analyze” workflow may constitute a workflow improvement to provide significant flexibility without impact on basophil reactivity.
doi_str_mv 10.1002/cyto.b.22172
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The focus now revolves around democratizing this tool, but, as blood sample stability could be challenging, after having developed a simplified approach, herein, we aimed to characterize two strategies for implementing BAT in multicentric studies: store and ship blood before or after sample processing. Fresh heparin‐ and EDTA‐anticoagulated whole blood samples followed both BAT workflows: “collect, store, process & analyze” or “collect, process, store & analyze”. Storage temperatures of 18–25 °C or 2–8 °C and preservation times from 0 to 7 days were considered. Interleukin‐3 was also evaluated. With the “collect, store, process & analyze” workflow, heparin‐anticoagulated blood and 18–25 °C storage were better than other conditions. While remaining possible, basophil activation exhibited a possible reactivity decay after 24 h. Under the conditions tested, interleukin‐3 had no role in enhancing basophil reactivity after storage. 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1552-4957
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source Wiley-Blackwell Journals
subjects basophil activation testing
basophil reactivity
Blood
blood sample stability
Cytokines
Edetic acid
Ethylenediaminetetraacetic acids
Flow cytometry
Heparin
Impact analysis
Interleukins
interleukin‐3
Leukocytes (basophilic)
multicentric studies
Reactivity
Workflow
title Possible alternative strategies to implement basophil activation testing in multicentric studies
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