Nano zinc oxide mediated resuscitation of aged Cajanus cajan via modulating aquaporin, cell cycle regulatory genes and hormonal responses
Key message Nanoparticle pretreatment improved the health of aged Cajanus cajan seeds viz., regulation of redox status, gene expression, and restoration of hormonal homeostasis. Ageing deteriorates the quality of seeds by lowering their vigor and viability, and terminating with loss of germination....
Gespeichert in:
| Veröffentlicht in: | Plant cell reports 2024-04, Vol.43 (4), p.110-110, Article 110 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 110 |
|---|---|
| container_issue | 4 |
| container_start_page | 110 |
| container_title | Plant cell reports |
| container_volume | 43 |
| creator | Kaur, Rasleen Yadu, Bhumika Chauhan, Nagendra Singh Parihar, Arun Singh Keshavkant, S. |
| description | Key message
Nanoparticle pretreatment improved the health of aged
Cajanus cajan
seeds viz., regulation of redox status, gene expression, and restoration of hormonal homeostasis.
Ageing deteriorates the quality of seeds by lowering their vigor and viability, and terminating with loss of germination. These days, nanotechnology has been seen to revolutionize the agricultural sectors, and particularly nano zinc oxide (nZnO) has gained considerable interests due to its distinctive properties. The aim of the present work was to decipher the possibilities of using nZnO to rejuvenate accelerated aged (AA) seeds of
Cajanus cajan
. Both chemically (CnZnO) and green (GnZnO; synthesized using
Moringa oleifera
) fabricated nZnOs were characterized via standard techniques to interpret their purity, size, and shape. Experimental results revealed erratic germination with a decline in viability and membrane stability as outcomes of reactive oxygen intermediate (ROI) buildup in AA seeds. Application of nZnO substantially rebated the accrual of ROI, along with enhanced production of antioxidants, α-amylase activity, total sugar, protein and DNA content. Higher level of zinc was assessed qualitatively/ histologically and quantitatively in nZnO pulsed AA seeds, supporting germination without inducing toxicity. Meantime, augmentation in the gibberellic acid with a simultaneous reduction in the abscisic acid level were noted in nZnO invigorated seeds than that determined in the AA seeds, suggesting possible involvement of ROI in hormonal signalling. Furthermore, nZnO-subjected AA seeds unveiled differential expression of aquaporins and cell cycle regulatory genes. Summarizing, among CnZnO and GnZnO, later one holds better potential for a revival of AA seeds of
Cajanus cajan
by providing considerable tolerance against ageing-associated deterioration via recouping the cellular redox homeostasis, hormonal signaling, and alteration in expression patterns of aquaporin and cell cycle regulatory genes.
Graphical Abstract |
| doi_str_mv | 10.1007/s00299-024-03202-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3031134599</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3153591779</sourcerecordid><originalsourceid>FETCH-LOGICAL-c359t-dd50a2c684359f82dfe1034206c9ce9bde584b1c375e721eda6535b573f2a0043</originalsourceid><addsrcrecordid>eNqFkc9u1DAQxi0EokvhBTggS1w4NGX8L1kf0QoKUlUuIHGLvPYkeJXYWzupWN6At67TLSD1AKfRzPzmmxl9hLxkcM4AmrcZgGtdAZcVCA68Yo_IiknBKw7i22OygoazqmmYPCHPct4BlGZTPyUnYq1qyUCuyK8rEyL96YOl8Yd3SEd03kzoaMI8Z-snM_kYaOyo6Ut1Y3YmzJnaJdIbb-gY3TwUKPTUXM9mH5MPZ9TiMFB7sAMWoX4BYjrQHgNmaoKj32MaYzDDsmYfQ8b8nDzpzJDxxX08JV8_vP-y-Vhdfr74tHl3WVmh9FQ5p8BwW69lSbs1dx0yEJJDbbVFvXWo1nLLrGgUlvfRmVoJtVWN6LgBkOKUvDnq7lO8njFP7ejzcq4JGOfcClZ4zZpG_x8FwZiQSi_o6wfoLs6pPHhHga6XCwvFj5RNMeeEXbtPfjTp0DJoF0_bo6dt8bS987RlZejVvfS8Le78GfltYgHEEcilFXpMf3f_Q_YWbHCtXA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3030964206</pqid></control><display><type>article</type><title>Nano zinc oxide mediated resuscitation of aged Cajanus cajan via modulating aquaporin, cell cycle regulatory genes and hormonal responses</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Kaur, Rasleen ; Yadu, Bhumika ; Chauhan, Nagendra Singh ; Parihar, Arun Singh ; Keshavkant, S.</creator><creatorcontrib>Kaur, Rasleen ; Yadu, Bhumika ; Chauhan, Nagendra Singh ; Parihar, Arun Singh ; Keshavkant, S.</creatorcontrib><description>Key message
Nanoparticle pretreatment improved the health of aged
Cajanus cajan
seeds viz., regulation of redox status, gene expression, and restoration of hormonal homeostasis.
Ageing deteriorates the quality of seeds by lowering their vigor and viability, and terminating with loss of germination. These days, nanotechnology has been seen to revolutionize the agricultural sectors, and particularly nano zinc oxide (nZnO) has gained considerable interests due to its distinctive properties. The aim of the present work was to decipher the possibilities of using nZnO to rejuvenate accelerated aged (AA) seeds of
Cajanus cajan
. Both chemically (CnZnO) and green (GnZnO; synthesized using
Moringa oleifera
) fabricated nZnOs were characterized via standard techniques to interpret their purity, size, and shape. Experimental results revealed erratic germination with a decline in viability and membrane stability as outcomes of reactive oxygen intermediate (ROI) buildup in AA seeds. Application of nZnO substantially rebated the accrual of ROI, along with enhanced production of antioxidants, α-amylase activity, total sugar, protein and DNA content. Higher level of zinc was assessed qualitatively/ histologically and quantitatively in nZnO pulsed AA seeds, supporting germination without inducing toxicity. Meantime, augmentation in the gibberellic acid with a simultaneous reduction in the abscisic acid level were noted in nZnO invigorated seeds than that determined in the AA seeds, suggesting possible involvement of ROI in hormonal signalling. Furthermore, nZnO-subjected AA seeds unveiled differential expression of aquaporins and cell cycle regulatory genes. Summarizing, among CnZnO and GnZnO, later one holds better potential for a revival of AA seeds of
Cajanus cajan
by providing considerable tolerance against ageing-associated deterioration via recouping the cellular redox homeostasis, hormonal signaling, and alteration in expression patterns of aquaporin and cell cycle regulatory genes.
Graphical Abstract</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-024-03202-1</identifier><identifier>PMID: 38564104</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Abscisic acid ; Aging ; Agricultural industry ; alpha-amylase ; Aquaporins ; Biomedical and Life Sciences ; Biotechnology ; Cajanus ; Cajanus cajan ; Cell Biology ; Cell Cycle ; DNA ; Gene expression ; gene expression regulation ; Genes ; Genes, Regulator ; Germination ; Gibberellic acid ; Homeostasis ; Life Sciences ; Moringa oleifera ; Nanoparticles ; Nanotechnology ; Original Article ; Plant Biochemistry ; Plant Sciences ; Reactive oxygen species ; Seeds ; sugars ; Toxicity ; viability ; vigor ; zinc ; Zinc oxide ; Zinc Oxide - pharmacology ; Zinc oxides ; α-Amylase</subject><ispartof>Plant cell reports, 2024-04, Vol.43 (4), p.110-110, Article 110</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c359t-dd50a2c684359f82dfe1034206c9ce9bde584b1c375e721eda6535b573f2a0043</cites><orcidid>0000-0003-3679-479X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00299-024-03202-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00299-024-03202-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27911,27912,41475,42544,51306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38564104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaur, Rasleen</creatorcontrib><creatorcontrib>Yadu, Bhumika</creatorcontrib><creatorcontrib>Chauhan, Nagendra Singh</creatorcontrib><creatorcontrib>Parihar, Arun Singh</creatorcontrib><creatorcontrib>Keshavkant, S.</creatorcontrib><title>Nano zinc oxide mediated resuscitation of aged Cajanus cajan via modulating aquaporin, cell cycle regulatory genes and hormonal responses</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><addtitle>Plant Cell Rep</addtitle><description>Key message
Nanoparticle pretreatment improved the health of aged
Cajanus cajan
seeds viz., regulation of redox status, gene expression, and restoration of hormonal homeostasis.
Ageing deteriorates the quality of seeds by lowering their vigor and viability, and terminating with loss of germination. These days, nanotechnology has been seen to revolutionize the agricultural sectors, and particularly nano zinc oxide (nZnO) has gained considerable interests due to its distinctive properties. The aim of the present work was to decipher the possibilities of using nZnO to rejuvenate accelerated aged (AA) seeds of
Cajanus cajan
. Both chemically (CnZnO) and green (GnZnO; synthesized using
Moringa oleifera
) fabricated nZnOs were characterized via standard techniques to interpret their purity, size, and shape. Experimental results revealed erratic germination with a decline in viability and membrane stability as outcomes of reactive oxygen intermediate (ROI) buildup in AA seeds. Application of nZnO substantially rebated the accrual of ROI, along with enhanced production of antioxidants, α-amylase activity, total sugar, protein and DNA content. Higher level of zinc was assessed qualitatively/ histologically and quantitatively in nZnO pulsed AA seeds, supporting germination without inducing toxicity. Meantime, augmentation in the gibberellic acid with a simultaneous reduction in the abscisic acid level were noted in nZnO invigorated seeds than that determined in the AA seeds, suggesting possible involvement of ROI in hormonal signalling. Furthermore, nZnO-subjected AA seeds unveiled differential expression of aquaporins and cell cycle regulatory genes. Summarizing, among CnZnO and GnZnO, later one holds better potential for a revival of AA seeds of
Cajanus cajan
by providing considerable tolerance against ageing-associated deterioration via recouping the cellular redox homeostasis, hormonal signaling, and alteration in expression patterns of aquaporin and cell cycle regulatory genes.
Graphical Abstract</description><subject>Abscisic acid</subject><subject>Aging</subject><subject>Agricultural industry</subject><subject>alpha-amylase</subject><subject>Aquaporins</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cajanus</subject><subject>Cajanus cajan</subject><subject>Cell Biology</subject><subject>Cell Cycle</subject><subject>DNA</subject><subject>Gene expression</subject><subject>gene expression regulation</subject><subject>Genes</subject><subject>Genes, Regulator</subject><subject>Germination</subject><subject>Gibberellic acid</subject><subject>Homeostasis</subject><subject>Life Sciences</subject><subject>Moringa oleifera</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Original Article</subject><subject>Plant Biochemistry</subject><subject>Plant Sciences</subject><subject>Reactive oxygen species</subject><subject>Seeds</subject><subject>sugars</subject><subject>Toxicity</subject><subject>viability</subject><subject>vigor</subject><subject>zinc</subject><subject>Zinc oxide</subject><subject>Zinc Oxide - pharmacology</subject><subject>Zinc oxides</subject><subject>α-Amylase</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxi0EokvhBTggS1w4NGX8L1kf0QoKUlUuIHGLvPYkeJXYWzupWN6At67TLSD1AKfRzPzmmxl9hLxkcM4AmrcZgGtdAZcVCA68Yo_IiknBKw7i22OygoazqmmYPCHPct4BlGZTPyUnYq1qyUCuyK8rEyL96YOl8Yd3SEd03kzoaMI8Z-snM_kYaOyo6Ut1Y3YmzJnaJdIbb-gY3TwUKPTUXM9mH5MPZ9TiMFB7sAMWoX4BYjrQHgNmaoKj32MaYzDDsmYfQ8b8nDzpzJDxxX08JV8_vP-y-Vhdfr74tHl3WVmh9FQ5p8BwW69lSbs1dx0yEJJDbbVFvXWo1nLLrGgUlvfRmVoJtVWN6LgBkOKUvDnq7lO8njFP7ejzcq4JGOfcClZ4zZpG_x8FwZiQSi_o6wfoLs6pPHhHga6XCwvFj5RNMeeEXbtPfjTp0DJoF0_bo6dt8bS987RlZejVvfS8Le78GfltYgHEEcilFXpMf3f_Q_YWbHCtXA</recordid><startdate>20240401</startdate><enddate>20240401</enddate><creator>Kaur, Rasleen</creator><creator>Yadu, Bhumika</creator><creator>Chauhan, Nagendra Singh</creator><creator>Parihar, Arun Singh</creator><creator>Keshavkant, S.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0003-3679-479X</orcidid></search><sort><creationdate>20240401</creationdate><title>Nano zinc oxide mediated resuscitation of aged Cajanus cajan via modulating aquaporin, cell cycle regulatory genes and hormonal responses</title><author>Kaur, Rasleen ; Yadu, Bhumika ; Chauhan, Nagendra Singh ; Parihar, Arun Singh ; Keshavkant, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-dd50a2c684359f82dfe1034206c9ce9bde584b1c375e721eda6535b573f2a0043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Abscisic acid</topic><topic>Aging</topic><topic>Agricultural industry</topic><topic>alpha-amylase</topic><topic>Aquaporins</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cajanus</topic><topic>Cajanus cajan</topic><topic>Cell Biology</topic><topic>Cell Cycle</topic><topic>DNA</topic><topic>Gene expression</topic><topic>gene expression regulation</topic><topic>Genes</topic><topic>Genes, Regulator</topic><topic>Germination</topic><topic>Gibberellic acid</topic><topic>Homeostasis</topic><topic>Life Sciences</topic><topic>Moringa oleifera</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Original Article</topic><topic>Plant Biochemistry</topic><topic>Plant Sciences</topic><topic>Reactive oxygen species</topic><topic>Seeds</topic><topic>sugars</topic><topic>Toxicity</topic><topic>viability</topic><topic>vigor</topic><topic>zinc</topic><topic>Zinc oxide</topic><topic>Zinc Oxide - pharmacology</topic><topic>Zinc oxides</topic><topic>α-Amylase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaur, Rasleen</creatorcontrib><creatorcontrib>Yadu, Bhumika</creatorcontrib><creatorcontrib>Chauhan, Nagendra Singh</creatorcontrib><creatorcontrib>Parihar, Arun Singh</creatorcontrib><creatorcontrib>Keshavkant, S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaur, Rasleen</au><au>Yadu, Bhumika</au><au>Chauhan, Nagendra Singh</au><au>Parihar, Arun Singh</au><au>Keshavkant, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nano zinc oxide mediated resuscitation of aged Cajanus cajan via modulating aquaporin, cell cycle regulatory genes and hormonal responses</atitle><jtitle>Plant cell reports</jtitle><stitle>Plant Cell Rep</stitle><addtitle>Plant Cell Rep</addtitle><date>2024-04-01</date><risdate>2024</risdate><volume>43</volume><issue>4</issue><spage>110</spage><epage>110</epage><pages>110-110</pages><artnum>110</artnum><issn>0721-7714</issn><eissn>1432-203X</eissn><abstract>Key message
Nanoparticle pretreatment improved the health of aged
Cajanus cajan
seeds viz., regulation of redox status, gene expression, and restoration of hormonal homeostasis.
Ageing deteriorates the quality of seeds by lowering their vigor and viability, and terminating with loss of germination. These days, nanotechnology has been seen to revolutionize the agricultural sectors, and particularly nano zinc oxide (nZnO) has gained considerable interests due to its distinctive properties. The aim of the present work was to decipher the possibilities of using nZnO to rejuvenate accelerated aged (AA) seeds of
Cajanus cajan
. Both chemically (CnZnO) and green (GnZnO; synthesized using
Moringa oleifera
) fabricated nZnOs were characterized via standard techniques to interpret their purity, size, and shape. Experimental results revealed erratic germination with a decline in viability and membrane stability as outcomes of reactive oxygen intermediate (ROI) buildup in AA seeds. Application of nZnO substantially rebated the accrual of ROI, along with enhanced production of antioxidants, α-amylase activity, total sugar, protein and DNA content. Higher level of zinc was assessed qualitatively/ histologically and quantitatively in nZnO pulsed AA seeds, supporting germination without inducing toxicity. Meantime, augmentation in the gibberellic acid with a simultaneous reduction in the abscisic acid level were noted in nZnO invigorated seeds than that determined in the AA seeds, suggesting possible involvement of ROI in hormonal signalling. Furthermore, nZnO-subjected AA seeds unveiled differential expression of aquaporins and cell cycle regulatory genes. Summarizing, among CnZnO and GnZnO, later one holds better potential for a revival of AA seeds of
Cajanus cajan
by providing considerable tolerance against ageing-associated deterioration via recouping the cellular redox homeostasis, hormonal signaling, and alteration in expression patterns of aquaporin and cell cycle regulatory genes.
Graphical Abstract</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>38564104</pmid><doi>10.1007/s00299-024-03202-1</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-3679-479X</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0721-7714 |
| ispartof | Plant cell reports, 2024-04, Vol.43 (4), p.110-110, Article 110 |
| issn | 0721-7714 1432-203X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3031134599 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Abscisic acid Aging Agricultural industry alpha-amylase Aquaporins Biomedical and Life Sciences Biotechnology Cajanus Cajanus cajan Cell Biology Cell Cycle DNA Gene expression gene expression regulation Genes Genes, Regulator Germination Gibberellic acid Homeostasis Life Sciences Moringa oleifera Nanoparticles Nanotechnology Original Article Plant Biochemistry Plant Sciences Reactive oxygen species Seeds sugars Toxicity viability vigor zinc Zinc oxide Zinc Oxide - pharmacology Zinc oxides α-Amylase |
| title | Nano zinc oxide mediated resuscitation of aged Cajanus cajan via modulating aquaporin, cell cycle regulatory genes and hormonal responses |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T17%3A26%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nano%20zinc%20oxide%20mediated%20resuscitation%20of%20aged%20Cajanus%20cajan%20via%20modulating%20aquaporin,%20cell%20cycle%20regulatory%20genes%20and%20hormonal%20responses&rft.jtitle=Plant%20cell%20reports&rft.au=Kaur,%20Rasleen&rft.date=2024-04-01&rft.volume=43&rft.issue=4&rft.spage=110&rft.epage=110&rft.pages=110-110&rft.artnum=110&rft.issn=0721-7714&rft.eissn=1432-203X&rft_id=info:doi/10.1007/s00299-024-03202-1&rft_dat=%3Cproquest_cross%3E3153591779%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3030964206&rft_id=info:pmid/38564104&rfr_iscdi=true |