EgMADS3 directly regulates EgLPAAT to mediate medium-chain fatty acids (MCFA) anabolism in the mesocarp of oil palm
Key message EgMADS3, a pivotal transcription factor, positively regulates MCFA accumulation via binding to the EgLPAAT promoter, advancing lipid content in mesocarp of oil palm. Lipids function as the structural components of cell membranes, which serve as permeable barriers to the external environm...
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| Veröffentlicht in: | Plant cell reports 2024-04, Vol.43 (4), p.107-107, Article 107 |
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| creator | Wang, Yaning Yan, Jinqi Yang, Ming Zou, Jixin Zheng, Yusheng Li, Dongdong |
| description | Key message
EgMADS3, a pivotal transcription factor, positively regulates MCFA accumulation via binding to the EgLPAAT promoter, advancing lipid content in mesocarp of oil palm.
Lipids function as the structural components of cell membranes, which serve as permeable barriers to the external environment of cells. The medium-chain fatty acid in the stored lipids of plants is an important renewable energy. Most research on MCFA production in plant lipid synthesis is based on biochemical methods, and the importance of transcriptional regulation in MCFA synthesis and its incorporation into TAGs needs further research. Oil palm is the most productive oil crop in the world and has the highest productivity among the main oil crops. In this study, the MADS transcription factor (
EgMADS3
) in the mesocarp of oil palm was characterized. Through the VIGS-virus induced gene silencing, it was determined that the potential target gene of
EgMADS3
was related to the biosynthesis of medium-chain fatty acid (MCFA). Transient transformation in protoplasts and qRT-PCR analysis showed that
EgMADS3
positively regulated the expression of
EgLPAAT
. The results of the yeast one-hybrid assays and EMSA indicated the interaction between
EgMADS3
and
EgLPAAT
promoter. Through genetic transformation and fatty acid analysis, it is concluded that
EgMADS3
directly regulates the mid-chain fatty acid synthesis pathway of the potential target gene
EgLPAAT
, thus promotes the accumulation of MCFA and improves the total lipid content. This study is innovative in the functional analysis of the MADS family transcription factor in the metabolism of medium-chain fatty acids (MCFA) of oil palm, provides a certain research basis for improving the metabolic pathway of chain fatty acids in oil palm, and improves the synthesis of MCFA in plants. Our results will provide a reference direction for further research on improving the oil quality through biotechnology of oil palm. |
| doi_str_mv | 10.1007/s00299-024-03200-3 |
| format | Article |
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EgMADS3, a pivotal transcription factor, positively regulates MCFA accumulation via binding to the EgLPAAT promoter, advancing lipid content in mesocarp of oil palm.
Lipids function as the structural components of cell membranes, which serve as permeable barriers to the external environment of cells. The medium-chain fatty acid in the stored lipids of plants is an important renewable energy. Most research on MCFA production in plant lipid synthesis is based on biochemical methods, and the importance of transcriptional regulation in MCFA synthesis and its incorporation into TAGs needs further research. Oil palm is the most productive oil crop in the world and has the highest productivity among the main oil crops. In this study, the MADS transcription factor (
EgMADS3
) in the mesocarp of oil palm was characterized. Through the VIGS-virus induced gene silencing, it was determined that the potential target gene of
EgMADS3
was related to the biosynthesis of medium-chain fatty acid (MCFA). Transient transformation in protoplasts and qRT-PCR analysis showed that
EgMADS3
positively regulated the expression of
EgLPAAT
. The results of the yeast one-hybrid assays and EMSA indicated the interaction between
EgMADS3
and
EgLPAAT
promoter. Through genetic transformation and fatty acid analysis, it is concluded that
EgMADS3
directly regulates the mid-chain fatty acid synthesis pathway of the potential target gene
EgLPAAT
, thus promotes the accumulation of MCFA and improves the total lipid content. This study is innovative in the functional analysis of the MADS family transcription factor in the metabolism of medium-chain fatty acids (MCFA) of oil palm, provides a certain research basis for improving the metabolic pathway of chain fatty acids in oil palm, and improves the synthesis of MCFA in plants. Our results will provide a reference direction for further research on improving the oil quality through biotechnology of oil palm.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-024-03200-3</identifier><identifier>PMID: 38558250</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Accumulation ; Biomedical and Life Sciences ; Biosynthesis ; Biotechnology ; Cell Biology ; Cell membranes ; Fatty acids ; Functional analysis ; Gene regulation ; Gene silencing ; Genetic transformation ; Life Sciences ; Lipids ; Metabolic pathways ; Oils & fats ; Oilseeds ; Original Article ; Plant Biochemistry ; Plant Sciences ; Protoplasts ; Renewable energy ; Transcription factors ; Vegetable oils ; Yeasts</subject><ispartof>Plant cell reports, 2024-04, Vol.43 (4), p.107-107, Article 107</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-99c31235c19022db99ea9567788a267c1bf5a3b5b7fa8b0a0694f3fdf529478f3</citedby><cites>FETCH-LOGICAL-c375t-99c31235c19022db99ea9567788a267c1bf5a3b5b7fa8b0a0694f3fdf529478f3</cites><orcidid>0000-0003-4006-3551</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00299-024-03200-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00299-024-03200-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38558250$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Yaning</creatorcontrib><creatorcontrib>Yan, Jinqi</creatorcontrib><creatorcontrib>Yang, Ming</creatorcontrib><creatorcontrib>Zou, Jixin</creatorcontrib><creatorcontrib>Zheng, Yusheng</creatorcontrib><creatorcontrib>Li, Dongdong</creatorcontrib><title>EgMADS3 directly regulates EgLPAAT to mediate medium-chain fatty acids (MCFA) anabolism in the mesocarp of oil palm</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><addtitle>Plant Cell Rep</addtitle><description>Key message
EgMADS3, a pivotal transcription factor, positively regulates MCFA accumulation via binding to the EgLPAAT promoter, advancing lipid content in mesocarp of oil palm.
Lipids function as the structural components of cell membranes, which serve as permeable barriers to the external environment of cells. The medium-chain fatty acid in the stored lipids of plants is an important renewable energy. Most research on MCFA production in plant lipid synthesis is based on biochemical methods, and the importance of transcriptional regulation in MCFA synthesis and its incorporation into TAGs needs further research. Oil palm is the most productive oil crop in the world and has the highest productivity among the main oil crops. In this study, the MADS transcription factor (
EgMADS3
) in the mesocarp of oil palm was characterized. Through the VIGS-virus induced gene silencing, it was determined that the potential target gene of
EgMADS3
was related to the biosynthesis of medium-chain fatty acid (MCFA). Transient transformation in protoplasts and qRT-PCR analysis showed that
EgMADS3
positively regulated the expression of
EgLPAAT
. The results of the yeast one-hybrid assays and EMSA indicated the interaction between
EgMADS3
and
EgLPAAT
promoter. Through genetic transformation and fatty acid analysis, it is concluded that
EgMADS3
directly regulates the mid-chain fatty acid synthesis pathway of the potential target gene
EgLPAAT
, thus promotes the accumulation of MCFA and improves the total lipid content. This study is innovative in the functional analysis of the MADS family transcription factor in the metabolism of medium-chain fatty acids (MCFA) of oil palm, provides a certain research basis for improving the metabolic pathway of chain fatty acids in oil palm, and improves the synthesis of MCFA in plants. Our results will provide a reference direction for further research on improving the oil quality through biotechnology of oil palm.</description><subject>Accumulation</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Cell membranes</subject><subject>Fatty acids</subject><subject>Functional analysis</subject><subject>Gene regulation</subject><subject>Gene silencing</subject><subject>Genetic transformation</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Metabolic pathways</subject><subject>Oils & fats</subject><subject>Oilseeds</subject><subject>Original Article</subject><subject>Plant Biochemistry</subject><subject>Plant Sciences</subject><subject>Protoplasts</subject><subject>Renewable energy</subject><subject>Transcription factors</subject><subject>Vegetable oils</subject><subject>Yeasts</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kc1q3DAUhUVIaabTvkAWRZBNslB7JVmWtTSTSVOY0EJT6E7IsjRxsK2pZC_m7aNk8gNddHVB5ztHl3sQOqXwhQLIrwmAKUWAFQQ4AyD8CC1owRlhwP8cowVIRomUtDhBH1K6B8iiLN-jE14JUTEBC5TW25v68hfHbRednfo9jm4792ZyCa-3m591fYungAfXdvntac4DsXemG7E307THxnZtwuc3q6v6ApvRNKHv0oCzPt09GlKwJu5w8Dh0Pd6ZfviI3nnTJ_fpeS7R76v17eqabH58-76qN8RyKSailOWUcWGpAsbaRilnlCilrCrDSmlp44XhjWikN1UDBkpVeO5bL5gqZOX5Ep0fcncx_J1dmvTQJev63owuzElz4JRyVua5RGf_oPdhjmPeLlOsyucVVGSKHSgbQ0rReb2L3WDiXlPQj5XoQyU6V6KfKtE8mz4_R89NPt-r5aWDDPADkLI0bl18-_s_sQ8kcJQj</recordid><startdate>20240401</startdate><enddate>20240401</enddate><creator>Wang, Yaning</creator><creator>Yan, Jinqi</creator><creator>Yang, Ming</creator><creator>Zou, Jixin</creator><creator>Zheng, Yusheng</creator><creator>Li, Dongdong</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4006-3551</orcidid></search><sort><creationdate>20240401</creationdate><title>EgMADS3 directly regulates EgLPAAT to mediate medium-chain fatty acids (MCFA) anabolism in the mesocarp of oil palm</title><author>Wang, Yaning ; Yan, Jinqi ; Yang, Ming ; Zou, Jixin ; Zheng, Yusheng ; Li, Dongdong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-99c31235c19022db99ea9567788a267c1bf5a3b5b7fa8b0a0694f3fdf529478f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Accumulation</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Cell membranes</topic><topic>Fatty acids</topic><topic>Functional analysis</topic><topic>Gene regulation</topic><topic>Gene silencing</topic><topic>Genetic transformation</topic><topic>Life Sciences</topic><topic>Lipids</topic><topic>Metabolic pathways</topic><topic>Oils & fats</topic><topic>Oilseeds</topic><topic>Original Article</topic><topic>Plant Biochemistry</topic><topic>Plant Sciences</topic><topic>Protoplasts</topic><topic>Renewable energy</topic><topic>Transcription factors</topic><topic>Vegetable oils</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yaning</creatorcontrib><creatorcontrib>Yan, Jinqi</creatorcontrib><creatorcontrib>Yang, Ming</creatorcontrib><creatorcontrib>Zou, Jixin</creatorcontrib><creatorcontrib>Zheng, Yusheng</creatorcontrib><creatorcontrib>Li, Dongdong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yaning</au><au>Yan, Jinqi</au><au>Yang, Ming</au><au>Zou, Jixin</au><au>Zheng, Yusheng</au><au>Li, Dongdong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>EgMADS3 directly regulates EgLPAAT to mediate medium-chain fatty acids (MCFA) anabolism in the mesocarp of oil palm</atitle><jtitle>Plant cell reports</jtitle><stitle>Plant Cell Rep</stitle><addtitle>Plant Cell Rep</addtitle><date>2024-04-01</date><risdate>2024</risdate><volume>43</volume><issue>4</issue><spage>107</spage><epage>107</epage><pages>107-107</pages><artnum>107</artnum><issn>0721-7714</issn><eissn>1432-203X</eissn><abstract>Key message
EgMADS3, a pivotal transcription factor, positively regulates MCFA accumulation via binding to the EgLPAAT promoter, advancing lipid content in mesocarp of oil palm.
Lipids function as the structural components of cell membranes, which serve as permeable barriers to the external environment of cells. The medium-chain fatty acid in the stored lipids of plants is an important renewable energy. Most research on MCFA production in plant lipid synthesis is based on biochemical methods, and the importance of transcriptional regulation in MCFA synthesis and its incorporation into TAGs needs further research. Oil palm is the most productive oil crop in the world and has the highest productivity among the main oil crops. In this study, the MADS transcription factor (
EgMADS3
) in the mesocarp of oil palm was characterized. Through the VIGS-virus induced gene silencing, it was determined that the potential target gene of
EgMADS3
was related to the biosynthesis of medium-chain fatty acid (MCFA). Transient transformation in protoplasts and qRT-PCR analysis showed that
EgMADS3
positively regulated the expression of
EgLPAAT
. The results of the yeast one-hybrid assays and EMSA indicated the interaction between
EgMADS3
and
EgLPAAT
promoter. Through genetic transformation and fatty acid analysis, it is concluded that
EgMADS3
directly regulates the mid-chain fatty acid synthesis pathway of the potential target gene
EgLPAAT
, thus promotes the accumulation of MCFA and improves the total lipid content. This study is innovative in the functional analysis of the MADS family transcription factor in the metabolism of medium-chain fatty acids (MCFA) of oil palm, provides a certain research basis for improving the metabolic pathway of chain fatty acids in oil palm, and improves the synthesis of MCFA in plants. Our results will provide a reference direction for further research on improving the oil quality through biotechnology of oil palm.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>38558250</pmid><doi>10.1007/s00299-024-03200-3</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-4006-3551</orcidid></addata></record> |
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| subjects | Accumulation Biomedical and Life Sciences Biosynthesis Biotechnology Cell Biology Cell membranes Fatty acids Functional analysis Gene regulation Gene silencing Genetic transformation Life Sciences Lipids Metabolic pathways Oils & fats Oilseeds Original Article Plant Biochemistry Plant Sciences Protoplasts Renewable energy Transcription factors Vegetable oils Yeasts |
| title | EgMADS3 directly regulates EgLPAAT to mediate medium-chain fatty acids (MCFA) anabolism in the mesocarp of oil palm |
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