Improving trans-cleavage activity of CRISPR-Cas13a using engineered crRNA with a uridinylate-rich 5′-overhang

The engieering of Cas13a crRNA to enhance its binding affinity with the Cas enzyme or target is a promising method of improving the collateral cleavage efficiency of CRISPR-Cas13a systems, thereby amplifying the sensitivity of nucleic acid detection. An examination of the top-performing engineered c...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Biosensors & bioelectronics 2024-07, Vol.255, p.116239-116239, Article 116239
Hauptverfasser:	Yang, Yihan, Sun, Lingli, Zhao, Jianhong, Jiao, Yang, Han, Taoli, Zhou, Xiaohong
Format:	Artikel
Sprache:	eng
Schlagworte:	CRISPR-Cas13a Engineered crRNA Nucleic acid detection SARS-CoV-2 Trans-Cleavage activity
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!