Microbulbifer magnicolonia sp. nov., isolated from sediment of tidal flat located in Zhoushan, China
A Gram-stain-negative, aerobic, motile with flagella and rod- or ovoid-shaped bacterium, designated GG15 , was isolated from tidal flat sediment sampled in Zhoushan, Zhejiang Province. Strain GG15 grew at 20-40 °C (optimum, 30 °C), at pH 5.5-9.5 (optimum, pH 7.0-8.0) and with 1.0-10.0 % (w/v) NaCl (...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2024-03, Vol.74 (3) |
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| container_title | International journal of systematic and evolutionary microbiology |
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| creator | Tao, Lang-Ci Gao, Jia-Wei Su, Yue Zhang, Wen-Wu Cao, Ke Yu, Xiao-Yun Ma, Kuo-Jian Xu, Lin Xia, Xiao-Ming Sun, Cong |
| description | A Gram-stain-negative, aerobic, motile with flagella and rod- or ovoid-shaped bacterium, designated GG15
, was isolated from tidal flat sediment sampled in Zhoushan, Zhejiang Province. Strain GG15
grew at 20-40 °C (optimum, 30 °C), at pH 5.5-9.5 (optimum, pH 7.0-8.0) and with 1.0-10.0 % (w/v) NaCl (optimum, 1.5 %). Colony diameters ranged from 1 to 3 mm within the first week, reaching a maximum of 6-7 mm after 15 days of cultivation. Strain GG15
exhibited highest 16S rRNA gene sequence similarity to
CCM 7856
(98.1 %), with similarity to other species within the genus
ranging from 97.8 to 93.8 %. Similarity values to other genera were below 93.8 %. Strain GG15
exhibited positive activity for
-glucosidase, trypsin and chymotrypsin, whereas the reference strain showed negative activity. Chemotaxonomic analyses indicated that strain GG15
contained Q-8 as the sole respiratory quinone, C
(9.1 %), iso-C
(30.9 %) and iso-C
3-OH (7.2 %) as the predominant fatty acids, and phosphatidylethanolamine, phosphatidylglycerol, three unidentified lipids, four unidentified glycolipids, one unidentified phospholipid, two unidentified aminolipids and two unidentified aminophospholipids as the main polar lipids. The genome of strain GG15
was 4 307 641 bp long, comprising 3861 protein-coding genes. The G+C content of strain GG15
was 61.5 mol% based on its genomic sequence. Strain GG15
showed low digital DNA-DNA hybridization ( |
| doi_str_mv | 10.1099/ijsem.0.006310 |
| format | Article |
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, was isolated from tidal flat sediment sampled in Zhoushan, Zhejiang Province. Strain GG15
grew at 20-40 °C (optimum, 30 °C), at pH 5.5-9.5 (optimum, pH 7.0-8.0) and with 1.0-10.0 % (w/v) NaCl (optimum, 1.5 %). Colony diameters ranged from 1 to 3 mm within the first week, reaching a maximum of 6-7 mm after 15 days of cultivation. Strain GG15
exhibited highest 16S rRNA gene sequence similarity to
CCM 7856
(98.1 %), with similarity to other species within the genus
ranging from 97.8 to 93.8 %. Similarity values to other genera were below 93.8 %. Strain GG15
exhibited positive activity for
-glucosidase, trypsin and chymotrypsin, whereas the reference strain showed negative activity. Chemotaxonomic analyses indicated that strain GG15
contained Q-8 as the sole respiratory quinone, C
(9.1 %), iso-C
(30.9 %) and iso-C
3-OH (7.2 %) as the predominant fatty acids, and phosphatidylethanolamine, phosphatidylglycerol, three unidentified lipids, four unidentified glycolipids, one unidentified phospholipid, two unidentified aminolipids and two unidentified aminophospholipids as the main polar lipids. The genome of strain GG15
was 4 307 641 bp long, comprising 3861 protein-coding genes. The G+C content of strain GG15
was 61.5 mol% based on its genomic sequence. Strain GG15
showed low digital DNA-DNA hybridization (<70 %) and average nucleotide identity values (<95 %) with other
species. As a result, a novel species within the genus
, named
sp. nov., is proposed. The type strain is GG15
(MCCC 1K08802
=KCTC 8210
).</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijsem.0.006310</identifier><identifier>PMID: 38546457</identifier><language>eng</language><publisher>England</publisher><subject>Alteromonadaceae ; Bacterial Typing Techniques ; Base Composition ; China ; DNA, Bacterial - genetics ; Fatty Acids - chemistry ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA</subject><ispartof>International journal of systematic and evolutionary microbiology, 2024-03, Vol.74 (3)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c250t-b91d90228e6cd0a539bfcdba9cd1227cb7a8cefa699f27276e5510eaa419a443</cites><orcidid>0000-0003-1587-0634</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38546457$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tao, Lang-Ci</creatorcontrib><creatorcontrib>Gao, Jia-Wei</creatorcontrib><creatorcontrib>Su, Yue</creatorcontrib><creatorcontrib>Zhang, Wen-Wu</creatorcontrib><creatorcontrib>Cao, Ke</creatorcontrib><creatorcontrib>Yu, Xiao-Yun</creatorcontrib><creatorcontrib>Ma, Kuo-Jian</creatorcontrib><creatorcontrib>Xu, Lin</creatorcontrib><creatorcontrib>Xia, Xiao-Ming</creatorcontrib><creatorcontrib>Sun, Cong</creatorcontrib><title>Microbulbifer magnicolonia sp. nov., isolated from sediment of tidal flat located in Zhoushan, China</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>A Gram-stain-negative, aerobic, motile with flagella and rod- or ovoid-shaped bacterium, designated GG15
, was isolated from tidal flat sediment sampled in Zhoushan, Zhejiang Province. Strain GG15
grew at 20-40 °C (optimum, 30 °C), at pH 5.5-9.5 (optimum, pH 7.0-8.0) and with 1.0-10.0 % (w/v) NaCl (optimum, 1.5 %). Colony diameters ranged from 1 to 3 mm within the first week, reaching a maximum of 6-7 mm after 15 days of cultivation. Strain GG15
exhibited highest 16S rRNA gene sequence similarity to
CCM 7856
(98.1 %), with similarity to other species within the genus
ranging from 97.8 to 93.8 %. Similarity values to other genera were below 93.8 %. Strain GG15
exhibited positive activity for
-glucosidase, trypsin and chymotrypsin, whereas the reference strain showed negative activity. Chemotaxonomic analyses indicated that strain GG15
contained Q-8 as the sole respiratory quinone, C
(9.1 %), iso-C
(30.9 %) and iso-C
3-OH (7.2 %) as the predominant fatty acids, and phosphatidylethanolamine, phosphatidylglycerol, three unidentified lipids, four unidentified glycolipids, one unidentified phospholipid, two unidentified aminolipids and two unidentified aminophospholipids as the main polar lipids. The genome of strain GG15
was 4 307 641 bp long, comprising 3861 protein-coding genes. The G+C content of strain GG15
was 61.5 mol% based on its genomic sequence. Strain GG15
showed low digital DNA-DNA hybridization (<70 %) and average nucleotide identity values (<95 %) with other
species. As a result, a novel species within the genus
, named
sp. nov., is proposed. The type strain is GG15
(MCCC 1K08802
=KCTC 8210
).</description><subject>Alteromonadaceae</subject><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>China</subject><subject>DNA, Bacterial - genetics</subject><subject>Fatty Acids - chemistry</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kL1PwzAQxS0EolBYGZFHhjacncSJR1TxJRWxdGKxHH9QV45d4gSJ_57Qlk53uvvd072H0A2BjADn926TTJtBBsByAifoghSMzUvIi9NjT9kEXaa0ARgHAOdoktdlwYqyukD6zakuNoNvnDUdbuVncCr6GJzEaZvhEL-zGXYpetkbjW0XW5yMdq0JPY4W905Lj-24xT6qHeMC_ljHIa1lmOHF2gV5hc6s9MlcH-oUrZ4eV4uX-fL9-XXxsJwrWkI_bzjRHCitDVMaZJnzxirdSK40obRSTSVrZaxknFta0YqZsiRgpCwIl0WRT9HdXnbbxa_BpF60LinjvQxm_Efko38gQOpqRLM9OppPqTNWbDvXyu5HEBB_wYpdsALEPtjx4PagPTSt0Uf8P8n8F_82dXE</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Tao, Lang-Ci</creator><creator>Gao, Jia-Wei</creator><creator>Su, Yue</creator><creator>Zhang, Wen-Wu</creator><creator>Cao, Ke</creator><creator>Yu, Xiao-Yun</creator><creator>Ma, Kuo-Jian</creator><creator>Xu, Lin</creator><creator>Xia, Xiao-Ming</creator><creator>Sun, Cong</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1587-0634</orcidid></search><sort><creationdate>20240301</creationdate><title>Microbulbifer magnicolonia sp. nov., isolated from sediment of tidal flat located in Zhoushan, China</title><author>Tao, Lang-Ci ; Gao, Jia-Wei ; Su, Yue ; Zhang, Wen-Wu ; Cao, Ke ; Yu, Xiao-Yun ; Ma, Kuo-Jian ; Xu, Lin ; Xia, Xiao-Ming ; Sun, Cong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c250t-b91d90228e6cd0a539bfcdba9cd1227cb7a8cefa699f27276e5510eaa419a443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Alteromonadaceae</topic><topic>Bacterial Typing Techniques</topic><topic>Base Composition</topic><topic>China</topic><topic>DNA, Bacterial - genetics</topic><topic>Fatty Acids - chemistry</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tao, Lang-Ci</creatorcontrib><creatorcontrib>Gao, Jia-Wei</creatorcontrib><creatorcontrib>Su, Yue</creatorcontrib><creatorcontrib>Zhang, Wen-Wu</creatorcontrib><creatorcontrib>Cao, Ke</creatorcontrib><creatorcontrib>Yu, Xiao-Yun</creatorcontrib><creatorcontrib>Ma, Kuo-Jian</creatorcontrib><creatorcontrib>Xu, Lin</creatorcontrib><creatorcontrib>Xia, Xiao-Ming</creatorcontrib><creatorcontrib>Sun, Cong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tao, Lang-Ci</au><au>Gao, Jia-Wei</au><au>Su, Yue</au><au>Zhang, Wen-Wu</au><au>Cao, Ke</au><au>Yu, Xiao-Yun</au><au>Ma, Kuo-Jian</au><au>Xu, Lin</au><au>Xia, Xiao-Ming</au><au>Sun, Cong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microbulbifer magnicolonia sp. nov., isolated from sediment of tidal flat located in Zhoushan, China</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2024-03-01</date><risdate>2024</risdate><volume>74</volume><issue>3</issue><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>A Gram-stain-negative, aerobic, motile with flagella and rod- or ovoid-shaped bacterium, designated GG15
, was isolated from tidal flat sediment sampled in Zhoushan, Zhejiang Province. Strain GG15
grew at 20-40 °C (optimum, 30 °C), at pH 5.5-9.5 (optimum, pH 7.0-8.0) and with 1.0-10.0 % (w/v) NaCl (optimum, 1.5 %). Colony diameters ranged from 1 to 3 mm within the first week, reaching a maximum of 6-7 mm after 15 days of cultivation. Strain GG15
exhibited highest 16S rRNA gene sequence similarity to
CCM 7856
(98.1 %), with similarity to other species within the genus
ranging from 97.8 to 93.8 %. Similarity values to other genera were below 93.8 %. Strain GG15
exhibited positive activity for
-glucosidase, trypsin and chymotrypsin, whereas the reference strain showed negative activity. Chemotaxonomic analyses indicated that strain GG15
contained Q-8 as the sole respiratory quinone, C
(9.1 %), iso-C
(30.9 %) and iso-C
3-OH (7.2 %) as the predominant fatty acids, and phosphatidylethanolamine, phosphatidylglycerol, three unidentified lipids, four unidentified glycolipids, one unidentified phospholipid, two unidentified aminolipids and two unidentified aminophospholipids as the main polar lipids. The genome of strain GG15
was 4 307 641 bp long, comprising 3861 protein-coding genes. The G+C content of strain GG15
was 61.5 mol% based on its genomic sequence. Strain GG15
showed low digital DNA-DNA hybridization (<70 %) and average nucleotide identity values (<95 %) with other
species. As a result, a novel species within the genus
, named
sp. nov., is proposed. The type strain is GG15
(MCCC 1K08802
=KCTC 8210
).</abstract><cop>England</cop><pmid>38546457</pmid><doi>10.1099/ijsem.0.006310</doi><orcidid>https://orcid.org/0000-0003-1587-0634</orcidid></addata></record> |
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| source | MEDLINE; Microbiology Society |
| subjects | Alteromonadaceae Bacterial Typing Techniques Base Composition China DNA, Bacterial - genetics Fatty Acids - chemistry Phylogeny RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA |
| title | Microbulbifer magnicolonia sp. nov., isolated from sediment of tidal flat located in Zhoushan, China |
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