Screening California surface waters for estrogenic endocrine disrupting chemicals (EEDC) with a juvenile rainbow trout liver vitellogenin mRNA procedure

Concern regarding the occurrence of chemicals that disrupt endocrine system functions in aquatic species has heightened over the last 15 years. However, little attention has been given to monitoring for estrogenic endocrine disrupting chemicals (EEDCs) in California's freshwater ecosystems. The...

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Veröffentlicht in:The Science of the total environment 2007-10, Vol.385 (1), p.66-79
Hauptverfasser: de Vlaming, V., Biales, A., Riordan, D., Markiewicz, D., Holmes, R., Otis, P., Zander, R., Lazorchak, J.
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container_end_page 79
container_issue 1
container_start_page 66
container_title The Science of the total environment
container_volume 385
creator de Vlaming, V.
Biales, A.
Riordan, D.
Markiewicz, D.
Holmes, R.
Otis, P.
Zander, R.
Lazorchak, J.
description Concern regarding the occurrence of chemicals that disrupt endocrine system functions in aquatic species has heightened over the last 15 years. However, little attention has been given to monitoring for estrogenic endocrine disrupting chemicals (EEDCs) in California's freshwater ecosystems. The objective was to screen surface water samples for estrogenic activity using vitellogenin (Vtg) mRNA quantification in livers of juvenile rainbow trout by real-time reverse transcriptase polymerase chain reaction (Q-RT PCR). Vtg mRNA analysis of livers from fish exposed to 113 ambient water samples collected from surface waters in California's Central Valley and northern area indicated that six samples (5% of total) may have contained EEDCs. The six samples induced marginal, but statistically significant, increases of Vtg mRNA. No ambient water sample evoked Vtg mRNA responses equivalent to those in positive controls (all responses were less than 2% of the positive control response). Thus, EEDC concentrations in these samples were low (at or near the threshold for the procedure) or results may have included false positives. To establish a more definitive assessment of EEDC occurrence, follow-up screening at sites where statistically significant, but weak, estrogenic activity was observed is recommended. Overall, results reveal that a majority of the California surface waters tested were below EEDC detection threshold concentration for the screening procedure utilized.
doi_str_mv 10.1016/j.scitotenv.2007.06.026
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subjects Animal, plant and microbial ecology
Animals
Applied ecology
Biological and medical sciences
California
California surface waters
Ecotoxicology, biological effects of pollution
Endocrine Disruptors - analysis
Endocrine Disruptors - toxicity
Environmental Monitoring
Estrogenic endocrine disruption
Estrogens, Non-Steroidal - analysis
Estrogens, Non-Steroidal - toxicity
Fresh Water - chemistry
Fundamental and applied biological sciences. Psychology
General aspects
Liver - drug effects
Liver - metabolism
Oncorhynchus mykiss
Oncorhynchus mykiss - growth & development
Oncorhynchus mykiss - metabolism
Rainbow trout
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
Vitellogenin mRNA
Vitellogenins - biosynthesis
Water Pollutants, Chemical - analysis
Water Pollutants, Chemical - toxicity
title Screening California surface waters for estrogenic endocrine disrupting chemicals (EEDC) with a juvenile rainbow trout liver vitellogenin mRNA procedure
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