Screening California surface waters for estrogenic endocrine disrupting chemicals (EEDC) with a juvenile rainbow trout liver vitellogenin mRNA procedure
Concern regarding the occurrence of chemicals that disrupt endocrine system functions in aquatic species has heightened over the last 15 years. However, little attention has been given to monitoring for estrogenic endocrine disrupting chemicals (EEDCs) in California's freshwater ecosystems. The...
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description | Concern regarding the occurrence of chemicals that disrupt endocrine system functions in aquatic species has heightened over the last 15 years. However, little attention has been given to monitoring for estrogenic endocrine disrupting chemicals (EEDCs) in California's freshwater ecosystems. The objective was to screen surface water samples for estrogenic activity using vitellogenin (Vtg) mRNA quantification in livers of juvenile rainbow trout by real-time reverse transcriptase polymerase chain reaction (Q-RT PCR). Vtg mRNA analysis of livers from fish exposed to 113 ambient water samples collected from surface waters in California's Central Valley and northern area indicated that six samples (5% of total) may have contained EEDCs. The six samples induced marginal, but statistically significant, increases of Vtg mRNA. No ambient water sample evoked Vtg mRNA responses equivalent to those in positive controls (all responses were less than 2% of the positive control response). Thus, EEDC concentrations in these samples were low (at or near the threshold for the procedure) or results may have included false positives. To establish a more definitive assessment of EEDC occurrence, follow-up screening at sites where statistically significant, but weak, estrogenic activity was observed is recommended. Overall, results reveal that a majority of the California surface waters tested were below EEDC detection threshold concentration for the screening procedure utilized. |
doi_str_mv | 10.1016/j.scitotenv.2007.06.026 |
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However, little attention has been given to monitoring for estrogenic endocrine disrupting chemicals (EEDCs) in California's freshwater ecosystems. The objective was to screen surface water samples for estrogenic activity using vitellogenin (Vtg) mRNA quantification in livers of juvenile rainbow trout by real-time reverse transcriptase polymerase chain reaction (Q-RT PCR). Vtg mRNA analysis of livers from fish exposed to 113 ambient water samples collected from surface waters in California's Central Valley and northern area indicated that six samples (5% of total) may have contained EEDCs. The six samples induced marginal, but statistically significant, increases of Vtg mRNA. No ambient water sample evoked Vtg mRNA responses equivalent to those in positive controls (all responses were less than 2% of the positive control response). Thus, EEDC concentrations in these samples were low (at or near the threshold for the procedure) or results may have included false positives. To establish a more definitive assessment of EEDC occurrence, follow-up screening at sites where statistically significant, but weak, estrogenic activity was observed is recommended. Overall, results reveal that a majority of the California surface waters tested were below EEDC detection threshold concentration for the screening procedure utilized.</description><identifier>ISSN: 0048-9697</identifier><identifier>EISSN: 1879-1026</identifier><identifier>DOI: 10.1016/j.scitotenv.2007.06.026</identifier><identifier>PMID: 17644162</identifier><identifier>CODEN: STENDL</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Animal, plant and microbial ecology ; Animals ; Applied ecology ; Biological and medical sciences ; California ; California surface waters ; Ecotoxicology, biological effects of pollution ; Endocrine Disruptors - analysis ; Endocrine Disruptors - toxicity ; Environmental Monitoring ; Estrogenic endocrine disruption ; Estrogens, Non-Steroidal - analysis ; Estrogens, Non-Steroidal - toxicity ; Fresh Water - chemistry ; Fundamental and applied biological sciences. Psychology ; General aspects ; Liver - drug effects ; Liver - metabolism ; Oncorhynchus mykiss ; Oncorhynchus mykiss - growth & development ; Oncorhynchus mykiss - metabolism ; Rainbow trout ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - biosynthesis ; Vitellogenin mRNA ; Vitellogenins - biosynthesis ; Water Pollutants, Chemical - analysis ; Water Pollutants, Chemical - toxicity</subject><ispartof>The Science of the total environment, 2007-10, Vol.385 (1), p.66-79</ispartof><rights>2007 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4566-4f0cedb7bce6e90724aca9e41179c669cd01f9a7878bfa98496d1d2004009a973</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.scitotenv.2007.06.026$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19101895$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17644162$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Vlaming, V.</creatorcontrib><creatorcontrib>Biales, A.</creatorcontrib><creatorcontrib>Riordan, D.</creatorcontrib><creatorcontrib>Markiewicz, D.</creatorcontrib><creatorcontrib>Holmes, R.</creatorcontrib><creatorcontrib>Otis, P.</creatorcontrib><creatorcontrib>Zander, R.</creatorcontrib><creatorcontrib>Lazorchak, J.</creatorcontrib><title>Screening California surface waters for estrogenic endocrine disrupting chemicals (EEDC) with a juvenile rainbow trout liver vitellogenin mRNA procedure</title><title>The Science of the total environment</title><addtitle>Sci Total Environ</addtitle><description>Concern regarding the occurrence of chemicals that disrupt endocrine system functions in aquatic species has heightened over the last 15 years. However, little attention has been given to monitoring for estrogenic endocrine disrupting chemicals (EEDCs) in California's freshwater ecosystems. The objective was to screen surface water samples for estrogenic activity using vitellogenin (Vtg) mRNA quantification in livers of juvenile rainbow trout by real-time reverse transcriptase polymerase chain reaction (Q-RT PCR). Vtg mRNA analysis of livers from fish exposed to 113 ambient water samples collected from surface waters in California's Central Valley and northern area indicated that six samples (5% of total) may have contained EEDCs. The six samples induced marginal, but statistically significant, increases of Vtg mRNA. No ambient water sample evoked Vtg mRNA responses equivalent to those in positive controls (all responses were less than 2% of the positive control response). Thus, EEDC concentrations in these samples were low (at or near the threshold for the procedure) or results may have included false positives. To establish a more definitive assessment of EEDC occurrence, follow-up screening at sites where statistically significant, but weak, estrogenic activity was observed is recommended. Overall, results reveal that a majority of the California surface waters tested were below EEDC detection threshold concentration for the screening procedure utilized.</description><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Applied ecology</subject><subject>Biological and medical sciences</subject><subject>California</subject><subject>California surface waters</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Endocrine Disruptors - analysis</subject><subject>Endocrine Disruptors - toxicity</subject><subject>Environmental Monitoring</subject><subject>Estrogenic endocrine disruption</subject><subject>Estrogens, Non-Steroidal - analysis</subject><subject>Estrogens, Non-Steroidal - toxicity</subject><subject>Fresh Water - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Oncorhynchus mykiss</subject><subject>Oncorhynchus mykiss - growth & development</subject><subject>Oncorhynchus mykiss - metabolism</subject><subject>Rainbow trout</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Vitellogenin mRNA</subject><subject>Vitellogenins - biosynthesis</subject><subject>Water Pollutants, Chemical - analysis</subject><subject>Water Pollutants, Chemical - toxicity</subject><issn>0048-9697</issn><issn>1879-1026</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd2O0zAQhS0EYsvCK4BvWLEXCeOs65_LqhQWaQUSP9eW40x2XaVOsZNUvAmPi0Mr9rK-sTX6zpnxHELeMCgZMPF-Wybnh37AMJUVgCxBlFCJJ2TBlNQFy--nZAHAVaGFlhfkRUpbyEcq9pxcMCk4Z6JakD_fXUQMPtzTte1828fgLU1jbK1DerADxkRzlWIaYn-fSUcxNL2LPiBtfIrjfpjV7gF33tku0XebzYf1NT344YFauh2nLOqQRutD3R9othkH2vkJI538gF33zzbQ3bcvK7qPvcNmjPiSPGuzG7463Zfk58fNj_Vtcff10-f16q5wfClEwVvIfC1rhwI1yIpbZzVyxqR2QmjXAGu1lUqqurVacS0a1uSVcQBttby5JFdH39z515h_aXY-uTyVDdiPydwAq4QS_CxYgVxqgOVZkHHFlJazozyCLvYpRWzNPvqdjb8NAzPHbLbmf8xmjtmAMDnarHx9ajHWO2wedadcM_D2BNiUQ2mjDc6nR05ne6XnWVdHDvOKJ49xboghr9RHdINpen92mL9R181S</recordid><startdate>20071015</startdate><enddate>20071015</enddate><creator>de Vlaming, V.</creator><creator>Biales, A.</creator><creator>Riordan, D.</creator><creator>Markiewicz, D.</creator><creator>Holmes, R.</creator><creator>Otis, P.</creator><creator>Zander, R.</creator><creator>Lazorchak, J.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7QH</scope><scope>7TM</scope><scope>7TN</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>8FD</scope><scope>FR3</scope><scope>KR7</scope></search><sort><creationdate>20071015</creationdate><title>Screening California surface waters for estrogenic endocrine disrupting chemicals (EEDC) with a juvenile rainbow trout liver vitellogenin mRNA procedure</title><author>de Vlaming, V. ; Biales, A. ; Riordan, D. ; Markiewicz, D. ; Holmes, R. ; Otis, P. ; Zander, R. ; Lazorchak, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4566-4f0cedb7bce6e90724aca9e41179c669cd01f9a7878bfa98496d1d2004009a973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Applied ecology</topic><topic>Biological and medical sciences</topic><topic>California</topic><topic>California surface waters</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Endocrine Disruptors - analysis</topic><topic>Endocrine Disruptors - toxicity</topic><topic>Environmental Monitoring</topic><topic>Estrogenic endocrine disruption</topic><topic>Estrogens, Non-Steroidal - analysis</topic><topic>Estrogens, Non-Steroidal - toxicity</topic><topic>Fresh Water - chemistry</topic><topic>Fundamental and applied biological sciences. 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However, little attention has been given to monitoring for estrogenic endocrine disrupting chemicals (EEDCs) in California's freshwater ecosystems. The objective was to screen surface water samples for estrogenic activity using vitellogenin (Vtg) mRNA quantification in livers of juvenile rainbow trout by real-time reverse transcriptase polymerase chain reaction (Q-RT PCR). Vtg mRNA analysis of livers from fish exposed to 113 ambient water samples collected from surface waters in California's Central Valley and northern area indicated that six samples (5% of total) may have contained EEDCs. The six samples induced marginal, but statistically significant, increases of Vtg mRNA. No ambient water sample evoked Vtg mRNA responses equivalent to those in positive controls (all responses were less than 2% of the positive control response). Thus, EEDC concentrations in these samples were low (at or near the threshold for the procedure) or results may have included false positives. To establish a more definitive assessment of EEDC occurrence, follow-up screening at sites where statistically significant, but weak, estrogenic activity was observed is recommended. Overall, results reveal that a majority of the California surface waters tested were below EEDC detection threshold concentration for the screening procedure utilized.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>17644162</pmid><doi>10.1016/j.scitotenv.2007.06.026</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animal, plant and microbial ecology Animals Applied ecology Biological and medical sciences California California surface waters Ecotoxicology, biological effects of pollution Endocrine Disruptors - analysis Endocrine Disruptors - toxicity Environmental Monitoring Estrogenic endocrine disruption Estrogens, Non-Steroidal - analysis Estrogens, Non-Steroidal - toxicity Fresh Water - chemistry Fundamental and applied biological sciences. Psychology General aspects Liver - drug effects Liver - metabolism Oncorhynchus mykiss Oncorhynchus mykiss - growth & development Oncorhynchus mykiss - metabolism Rainbow trout Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - biosynthesis Vitellogenin mRNA Vitellogenins - biosynthesis Water Pollutants, Chemical - analysis Water Pollutants, Chemical - toxicity |
title | Screening California surface waters for estrogenic endocrine disrupting chemicals (EEDC) with a juvenile rainbow trout liver vitellogenin mRNA procedure |
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