DNA purification silicon chip
A silicon microchip has been developed for purification of deoxyribonucleic acid (DNA) from human blood based on the known affinity of DNA to silica under high chaotropic salt conditions. The chip comprises several components which include a micromixer, microfilter, microbinder and two microvalves t...
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| Veröffentlicht in: | Sensors and actuators. A. Physical. 2007-09, Vol.139 (1), p.139-144 |
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| container_issue | 1 |
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| container_title | Sensors and actuators. A. Physical. |
| container_volume | 139 |
| creator | Ji, Hong Miao Samper, Victor Chen, Yu Hui, Wing Cheong Lye, Hui Jen Mustafa, Fatimah Bte Lee, Ai Cheng Cong, Lin Heng, Chew Kiat Lim, Tit Meng |
| description | A silicon microchip has been developed for purification of deoxyribonucleic acid (DNA) from human blood based on the known affinity of DNA to silica under high chaotropic salt conditions. The chip comprises several components which include a micromixer, microfilter, microbinder and two microvalves to perform the functions of the mixing, filtering, purification and fluidic control; and yet it can be accomplished by a simple mask process flow process. Original human blood sample which is a mixture of many components, including white blood cells (WBC), red blood cells (RBC), platelets, plasma, etc., can be used directly as the input sample. After cell sorting, cell lysing, DNA binding, eluting and cleaning processes, pure DNA has been extracted for the white blood cells. An average of 10
ng DNA/μl blood was able to be extracted from this fully integrated DNA extraction chip. The purity of the eluted DNA was evaluated by optical density at wavelengths of 260
nm and 280
nm. Tests results showed that the most DNA solution eluted was free from RNA and protein contamination. |
| doi_str_mv | 10.1016/j.sna.2007.05.033 |
| format | Article |
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ng DNA/μl blood was able to be extracted from this fully integrated DNA extraction chip. The purity of the eluted DNA was evaluated by optical density at wavelengths of 260
nm and 280
nm. Tests results showed that the most DNA solution eluted was free from RNA and protein contamination.</description><subject>DNA extraction</subject><subject>Microbinder</subject><subject>Microfilter</subject><subject>Micromixer</subject><subject>Microvalve</subject><subject>Preparation</subject><subject>Purification</subject><issn>0924-4247</issn><issn>1873-3069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp9kD1PwzAURS0EEqXwAxiQOrElvOdnx7aYKr6lChaYLddxhKs0CXaKxL8nVZmZ7nLPle5h7BKhRMDqZlPmzpUcQJUgSyA6YjPUigqCyhyzGRguCsGFOmVnOW8ApopSM3Z1_7pcDLsUm-jdGPtukWMb_ZT-Mw7n7KRxbQ4XfzlnH48P73fPxert6eVuuSo8cT0WXDoDUpFWjnOPQqNSa4ENmopqp4WURIbIq7WptAAleaXRoIMagxeKaM6uD7tD6r92IY92G7MPbeu60O-yJUAAnDbmDA9Fn_qcU2jskOLWpR-LYPci7MZOIuxehAVpp5cTc3tgwvTgO4Zks4-h86GOKfjR1n38h_4FLs9iHA</recordid><startdate>20070912</startdate><enddate>20070912</enddate><creator>Ji, Hong Miao</creator><creator>Samper, Victor</creator><creator>Chen, Yu</creator><creator>Hui, Wing Cheong</creator><creator>Lye, Hui Jen</creator><creator>Mustafa, Fatimah Bte</creator><creator>Lee, Ai Cheng</creator><creator>Cong, Lin</creator><creator>Heng, Chew Kiat</creator><creator>Lim, Tit Meng</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TB</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>L7M</scope></search><sort><creationdate>20070912</creationdate><title>DNA purification silicon chip</title><author>Ji, Hong Miao ; Samper, Victor ; Chen, Yu ; Hui, Wing Cheong ; Lye, Hui Jen ; Mustafa, Fatimah Bte ; Lee, Ai Cheng ; Cong, Lin ; Heng, Chew Kiat ; Lim, Tit Meng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c328t-25a9057387a22c148177b41f1963da845533933c7b9684075268191a0d1ec4733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>DNA extraction</topic><topic>Microbinder</topic><topic>Microfilter</topic><topic>Micromixer</topic><topic>Microvalve</topic><topic>Preparation</topic><topic>Purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ji, Hong Miao</creatorcontrib><creatorcontrib>Samper, Victor</creatorcontrib><creatorcontrib>Chen, Yu</creatorcontrib><creatorcontrib>Hui, Wing Cheong</creatorcontrib><creatorcontrib>Lye, Hui Jen</creatorcontrib><creatorcontrib>Mustafa, Fatimah Bte</creatorcontrib><creatorcontrib>Lee, Ai Cheng</creatorcontrib><creatorcontrib>Cong, Lin</creatorcontrib><creatorcontrib>Heng, Chew Kiat</creatorcontrib><creatorcontrib>Lim, Tit Meng</creatorcontrib><collection>CrossRef</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Sensors and actuators. A. Physical.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ji, Hong Miao</au><au>Samper, Victor</au><au>Chen, Yu</au><au>Hui, Wing Cheong</au><au>Lye, Hui Jen</au><au>Mustafa, Fatimah Bte</au><au>Lee, Ai Cheng</au><au>Cong, Lin</au><au>Heng, Chew Kiat</au><au>Lim, Tit Meng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA purification silicon chip</atitle><jtitle>Sensors and actuators. A. Physical.</jtitle><date>2007-09-12</date><risdate>2007</risdate><volume>139</volume><issue>1</issue><spage>139</spage><epage>144</epage><pages>139-144</pages><issn>0924-4247</issn><eissn>1873-3069</eissn><abstract>A silicon microchip has been developed for purification of deoxyribonucleic acid (DNA) from human blood based on the known affinity of DNA to silica under high chaotropic salt conditions. The chip comprises several components which include a micromixer, microfilter, microbinder and two microvalves to perform the functions of the mixing, filtering, purification and fluidic control; and yet it can be accomplished by a simple mask process flow process. Original human blood sample which is a mixture of many components, including white blood cells (WBC), red blood cells (RBC), platelets, plasma, etc., can be used directly as the input sample. After cell sorting, cell lysing, DNA binding, eluting and cleaning processes, pure DNA has been extracted for the white blood cells. An average of 10
ng DNA/μl blood was able to be extracted from this fully integrated DNA extraction chip. The purity of the eluted DNA was evaluated by optical density at wavelengths of 260
nm and 280
nm. Tests results showed that the most DNA solution eluted was free from RNA and protein contamination.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.sna.2007.05.033</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0924-4247 |
| ispartof | Sensors and actuators. A. Physical., 2007-09, Vol.139 (1), p.139-144 |
| issn | 0924-4247 1873-3069 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_30100139 |
| source | Access via ScienceDirect (Elsevier) |
| subjects | DNA extraction Microbinder Microfilter Micromixer Microvalve Preparation Purification |
| title | DNA purification silicon chip |
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