The Effects of the Various Surface Treatments of Titanium on Cellular Activity of Osteoblast-Like Cells
The response of osteoblast-like cells cultured on blasted and/or acid etching surfaces and the influence of surface texture or microtopography on cell attachment, cell proliferation, and the gene expression of the osteoblastic phenotype using ROS 17/2.8 cell lines were evaluated. The blasted and/or...
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| Veröffentlicht in: | Key engineering materials 2007-02, Vol.330-332, p.71-74 |
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| container_title | Key engineering materials |
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| creator | Suh, Jo Young Lee, H. Choi, Byung Ju Park, J.W. Yeo, S.I. |
| description | The response of osteoblast-like cells cultured on blasted and/or acid etching
surfaces and the influence of surface texture or microtopography on cell attachment, cell
proliferation, and the gene expression of the osteoblastic phenotype using ROS 17/2.8 cell
lines were evaluated. The blasted and/or acid etching surfaces were significantly rougher in
comparison to machined and etched surfaces (p < 0.05). On X-ray diffraction analysis,
titanium hydride (TiH2) was observed on the surface etched with a mixture of HCl-H2SO4
solution, whereas TiH2 was not observed on machined and blasted surfaces. After 24 h
incubation, most of the cells of all the groups had a flattened, polygonal shape and were fully
spread, exhibiting the onset of proliferation. The MTT assay showed a significant decrease on
the blasted surface compared to the machined surface at 7 day culture (p < 0.05). The
expression of osteopontin mRNA, α1 (I) collagen mRNA, and alkaline phosphatase mRNA
on rough surfaces was higher than on the machined surfaces, and was highest on the blasted
surface at day 7. |
| doi_str_mv | 10.4028/www.scientific.net/KEM.330-332.71 |
| format | Article |
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surfaces and the influence of surface texture or microtopography on cell attachment, cell
proliferation, and the gene expression of the osteoblastic phenotype using ROS 17/2.8 cell
lines were evaluated. The blasted and/or acid etching surfaces were significantly rougher in
comparison to machined and etched surfaces (p < 0.05). On X-ray diffraction analysis,
titanium hydride (TiH2) was observed on the surface etched with a mixture of HCl-H2SO4
solution, whereas TiH2 was not observed on machined and blasted surfaces. After 24 h
incubation, most of the cells of all the groups had a flattened, polygonal shape and were fully
spread, exhibiting the onset of proliferation. The MTT assay showed a significant decrease on
the blasted surface compared to the machined surface at 7 day culture (p < 0.05). The
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on rough surfaces was higher than on the machined surfaces, and was highest on the blasted
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surfaces and the influence of surface texture or microtopography on cell attachment, cell
proliferation, and the gene expression of the osteoblastic phenotype using ROS 17/2.8 cell
lines were evaluated. The blasted and/or acid etching surfaces were significantly rougher in
comparison to machined and etched surfaces (p < 0.05). On X-ray diffraction analysis,
titanium hydride (TiH2) was observed on the surface etched with a mixture of HCl-H2SO4
solution, whereas TiH2 was not observed on machined and blasted surfaces. After 24 h
incubation, most of the cells of all the groups had a flattened, polygonal shape and were fully
spread, exhibiting the onset of proliferation. The MTT assay showed a significant decrease on
the blasted surface compared to the machined surface at 7 day culture (p < 0.05). The
expression of osteopontin mRNA, α1 (I) collagen mRNA, and alkaline phosphatase mRNA
on rough surfaces was higher than on the machined surfaces, and was highest on the blasted
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surfaces and the influence of surface texture or microtopography on cell attachment, cell
proliferation, and the gene expression of the osteoblastic phenotype using ROS 17/2.8 cell
lines were evaluated. The blasted and/or acid etching surfaces were significantly rougher in
comparison to machined and etched surfaces (p < 0.05). On X-ray diffraction analysis,
titanium hydride (TiH2) was observed on the surface etched with a mixture of HCl-H2SO4
solution, whereas TiH2 was not observed on machined and blasted surfaces. After 24 h
incubation, most of the cells of all the groups had a flattened, polygonal shape and were fully
spread, exhibiting the onset of proliferation. The MTT assay showed a significant decrease on
the blasted surface compared to the machined surface at 7 day culture (p < 0.05). The
expression of osteopontin mRNA, α1 (I) collagen mRNA, and alkaline phosphatase mRNA
on rough surfaces was higher than on the machined surfaces, and was highest on the blasted
surface at day 7.</abstract><pub>Trans Tech Publications Ltd</pub><doi>10.4028/www.scientific.net/KEM.330-332.71</doi><tpages>4</tpages></addata></record> |
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| title | The Effects of the Various Surface Treatments of Titanium on Cellular Activity of Osteoblast-Like Cells |
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