Fusion PCR-targeted tylCV gene deletion of Streptomyces fradiae for producing desmycosin, the direct precursor of tilmicosin

Tilmicosin is a semisynthetic macrolide antibiotic derived from tylosin. The disruption of tylCV gene from tylosin producer— Streptomyces fradiae would result in the accumulation of desmycosin, the direct precursor of tilmicosin. TylCV gene disruption cassette was constructed by fusion PCR. The tylC...

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Veröffentlicht in:	Process biochemistry (1991) 2007-04, Vol.42 (4), p.729-733
Hauptverfasser:	Min, Yong, Lv, Heping, Zheng, Yinghua
Format:	Artikel
Sprache:	eng
Schlagworte:	Desmycosin FLP recombinase Fusion PCR Gene replacement Streptomyces fradiae Tilmicosin
Online-Zugang:	Volltext
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