Antimicrobial peptide functionalized gold nanorods combining near-infrared photothermal therapy for effective wound healing

Photothermal therapy using laser activated gold nanorods (AuNRs) is a strategy for treatment of bacterial infections. Nevertheless, it also exerts cytotoxicity against human cells which leads to adverse effects in healthy human tissues and limits the applicable dose. Functionalization of AuNRs with...

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Veröffentlicht in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2022-12, Vol.220, p.112887, Article 112887
Hauptverfasser: Xu, Xinyu, Ding, Yujie, Hadianamrei, Roja, Lv, Songwei, You, Rongrong, Pan, Fang, Zhang, Peng, Wang, Nan, Zhao, Xiubo
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container_start_page 112887
container_title Colloids and surfaces, B, Biointerfaces
container_volume 220
creator Xu, Xinyu
Ding, Yujie
Hadianamrei, Roja
Lv, Songwei
You, Rongrong
Pan, Fang
Zhang, Peng
Wang, Nan
Zhao, Xiubo
description Photothermal therapy using laser activated gold nanorods (AuNRs) is a strategy for treatment of bacterial infections. Nevertheless, it also exerts cytotoxicity against human cells which leads to adverse effects in healthy human tissues and limits the applicable dose. Functionalization of AuNRs with a selective antimicrobial peptide (AMP) with higher selectivity for bacteria over human cells is a promising strategy for increasing the selectivity of the AuNRs for bacteria, hence increasing their cellular uptake by the bacteria in order to achieve stronger antimicrobial effects with lower doses of AuNRs without damaging the human cells. In this study, the surface of AuNRs was functionalized with a short AMP named C-At5 and the efficiency of the peptide functionalized AuNRs in killing gram-positive and gram-negative bacteria was evaluated in vitro as well as their potential for facilitating wound healing in a mouse model of wound infection with and without application of laser. The peptide-conjugated AuNRs exhibited higher antibacterial activity in vitro compared to the plain AuNRs both in the presence and absence of laser irradiation. Furthermore, AuNR@C-At5 had very low toxicity against human skin fibroblasts and human red blood cells indicating their higher biocompatibility compared to the plain AuNRs. Treatment of wounded mice with AuNR@C-At5 accelerated the wound healing process which was further enhanced by applying laser. The system developed in this study has great potential for customization for specific antimicrobial or antifungal therapy via conjugation of different types of AMPs with higher selectivity and can therefore serve as a guide for any future attempts in this regard. [Display omitted] •The antimicrobial peptides were successfully conjugated to the AuNRs.•The conjugation of the peptides to the AuNRs reduced the toxicity of the peptides.•The AuNR@C-At5 exhibited promising antimicrobial and wound healing effects.•The laser irradiation enhanced the antimicrobial and wound healing effects.
doi_str_mv 10.1016/j.colsurfb.2022.112887
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Nevertheless, it also exerts cytotoxicity against human cells which leads to adverse effects in healthy human tissues and limits the applicable dose. Functionalization of AuNRs with a selective antimicrobial peptide (AMP) with higher selectivity for bacteria over human cells is a promising strategy for increasing the selectivity of the AuNRs for bacteria, hence increasing their cellular uptake by the bacteria in order to achieve stronger antimicrobial effects with lower doses of AuNRs without damaging the human cells. In this study, the surface of AuNRs was functionalized with a short AMP named C-At5 and the efficiency of the peptide functionalized AuNRs in killing gram-positive and gram-negative bacteria was evaluated in vitro as well as their potential for facilitating wound healing in a mouse model of wound infection with and without application of laser. 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The peptide-conjugated AuNRs exhibited higher antibacterial activity in vitro compared to the plain AuNRs both in the presence and absence of laser irradiation. Furthermore, AuNR@C-At5 had very low toxicity against human skin fibroblasts and human red blood cells indicating their higher biocompatibility compared to the plain AuNRs. Treatment of wounded mice with AuNR@C-At5 accelerated the wound healing process which was further enhanced by applying laser. The system developed in this study has great potential for customization for specific antimicrobial or antifungal therapy via conjugation of different types of AMPs with higher selectivity and can therefore serve as a guide for any future attempts in this regard. [Display omitted] •The antimicrobial peptides were successfully conjugated to the AuNRs.•The conjugation of the peptides to the AuNRs reduced the toxicity of the peptides.•The AuNR@C-At5 exhibited promising antimicrobial and wound healing effects.•The laser irradiation enhanced the antimicrobial and wound healing effects.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>36191410</pmid><doi>10.1016/j.colsurfb.2022.112887</doi></addata></record>
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subjects Animals
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - therapeutic use
antibacterial properties
Antimicrobial peptide
Antimicrobial Peptides
biocompatibility
blood
cytotoxicity
fibroblasts
gold
Gold - pharmacology
Gold - therapeutic use
Gold nanoparticles
Gram-Negative Bacteria
Gram-Positive Bacteria
Humans
irradiation
Mice
nanorods
Nanotubes
Peptides
Photothermal Therapy
photothermotherapy
skin (animal)
Wound Healing
title Antimicrobial peptide functionalized gold nanorods combining near-infrared photothermal therapy for effective wound healing
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