Monitoring faecal contamination of the Thames estuary using a semiautomated early warning system
The Colifast Early Warning System, based on measuring beta-galactosidase activity (2 h method), was evaluated for monitoring the level of faecal contamination in the upper tidal Thames. Two trials were performed, one following heavy rain in November 2000, the next during a dry and sunny period in Ju...
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| Veröffentlicht in: | Water science and technology 2002-01, Vol.46 (3), p.25-31 |
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| description | The Colifast Early Warning System, based on measuring beta-galactosidase activity (2 h method), was evaluated for monitoring the level of faecal contamination in the upper tidal Thames. Two trials were performed, one following heavy rain in November 2000, the next during a dry and sunny period in July 2001. In general the beta-galactosidase activity and the two coliform reference methods (recovery following membrane filtration with membrane lauryl sulphate broth (MLSB) and Colilert Quantitray) were comparable. However, in several samples in July the beta-galactosidase activity seemed to overestimate the number of culturable coliforms, suggesting that the rapid enzymatic method detected beta-galactosidase produced by other bacterial sources, such as Aeromonas spp. or Vibrio spp., or nonculturable coliforms. The later could be attributed to sunlight-induced injury. Nevertheless, the rapid method based on beta-galactosidase activity gave an estimate of the level of culturable coliforms, which did not differ from both coliform reference methods by more than one log. Monitoring of beta-galactosidase activity in river water samples using the Colifast Analyser may therefore be useful as an early warning indicator of faecal contamination. |
| doi_str_mv | 10.2166/wst.2002.0047 |
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D</creator><creatorcontrib>TRYLAND, I ; SURMAN, S ; BERG, J. D</creatorcontrib><description>The Colifast Early Warning System, based on measuring beta-galactosidase activity (2 h method), was evaluated for monitoring the level of faecal contamination in the upper tidal Thames. Two trials were performed, one following heavy rain in November 2000, the next during a dry and sunny period in July 2001. In general the beta-galactosidase activity and the two coliform reference methods (recovery following membrane filtration with membrane lauryl sulphate broth (MLSB) and Colilert Quantitray) were comparable. However, in several samples in July the beta-galactosidase activity seemed to overestimate the number of culturable coliforms, suggesting that the rapid enzymatic method detected beta-galactosidase produced by other bacterial sources, such as Aeromonas spp. or Vibrio spp., or nonculturable coliforms. The later could be attributed to sunlight-induced injury. Nevertheless, the rapid method based on beta-galactosidase activity gave an estimate of the level of culturable coliforms, which did not differ from both coliform reference methods by more than one log. Monitoring of beta-galactosidase activity in river water samples using the Colifast Analyser may therefore be useful as an early warning indicator of faecal contamination.</description><identifier>ISSN: 0273-1223</identifier><identifier>ISBN: 9781843394228</identifier><identifier>ISBN: 1843394227</identifier><identifier>EISSN: 1996-9732</identifier><identifier>DOI: 10.2166/wst.2002.0047</identifier><identifier>PMID: 12227600</identifier><identifier>CODEN: WSTED4</identifier><language>eng</language><publisher>London: IWA</publisher><subject>Aeromonas ; Applied sciences ; Automation ; Bacteria ; beta-Galactosidase - analysis ; beta-Galactosidase - pharmacology ; Biological Assay - methods ; Brackish ; British Isles, England, Thames R ; Cattle ; Coliforms ; Contamination ; Early warning systems ; Earth sciences ; Earth, ocean, space ; Engineering and environment geology. Geothermics ; England ; Enterobacteriaceae - isolation & purification ; Environmental monitoring ; Environmental Monitoring - methods ; Estuaries ; Estuarine dynamics ; Exact sciences and technology ; Feces ; Feces - enzymology ; Galactosidase ; Membrane filtration ; Methods ; Natural water pollution ; Pollution ; Pollution, environment geology ; Rain ; Rainfall ; River water ; Rivers ; Seasons ; Seawaters, estuaries ; Sensitivity and Specificity ; Vibrio ; Water analysis ; Water Pollutants - analysis ; Water sampling ; Water treatment and pollution ; Waterborne diseases ; β-Galactosidase</subject><ispartof>Water science and technology, 2002-01, Vol.46 (3), p.25-31</ispartof><rights>2002 INIST-CNRS</rights><rights>Copyright IWA Publishing Aug 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-6021433c758e3a8cee9f5bb983e55e73a2f204372c16eccc48081bc80a2443333</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,780,784,789,790,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14182513$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12227600$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TRYLAND, I</creatorcontrib><creatorcontrib>SURMAN, S</creatorcontrib><creatorcontrib>BERG, J. D</creatorcontrib><title>Monitoring faecal contamination of the Thames estuary using a semiautomated early warning system</title><title>Water science and technology</title><addtitle>Water Sci Technol</addtitle><description>The Colifast Early Warning System, based on measuring beta-galactosidase activity (2 h method), was evaluated for monitoring the level of faecal contamination in the upper tidal Thames. Two trials were performed, one following heavy rain in November 2000, the next during a dry and sunny period in July 2001. In general the beta-galactosidase activity and the two coliform reference methods (recovery following membrane filtration with membrane lauryl sulphate broth (MLSB) and Colilert Quantitray) were comparable. However, in several samples in July the beta-galactosidase activity seemed to overestimate the number of culturable coliforms, suggesting that the rapid enzymatic method detected beta-galactosidase produced by other bacterial sources, such as Aeromonas spp. or Vibrio spp., or nonculturable coliforms. The later could be attributed to sunlight-induced injury. Nevertheless, the rapid method based on beta-galactosidase activity gave an estimate of the level of culturable coliforms, which did not differ from both coliform reference methods by more than one log. Monitoring of beta-galactosidase activity in river water samples using the Colifast Analyser may therefore be useful as an early warning indicator of faecal contamination.</description><subject>Aeromonas</subject><subject>Applied sciences</subject><subject>Automation</subject><subject>Bacteria</subject><subject>beta-Galactosidase - analysis</subject><subject>beta-Galactosidase - pharmacology</subject><subject>Biological Assay - methods</subject><subject>Brackish</subject><subject>British Isles, England, Thames R</subject><subject>Cattle</subject><subject>Coliforms</subject><subject>Contamination</subject><subject>Early warning systems</subject><subject>Earth sciences</subject><subject>Earth, ocean, space</subject><subject>Engineering and environment geology. Geothermics</subject><subject>England</subject><subject>Enterobacteriaceae - isolation & purification</subject><subject>Environmental monitoring</subject><subject>Environmental Monitoring - methods</subject><subject>Estuaries</subject><subject>Estuarine dynamics</subject><subject>Exact sciences and technology</subject><subject>Feces</subject><subject>Feces - enzymology</subject><subject>Galactosidase</subject><subject>Membrane filtration</subject><subject>Methods</subject><subject>Natural water pollution</subject><subject>Pollution</subject><subject>Pollution, environment geology</subject><subject>Rain</subject><subject>Rainfall</subject><subject>River water</subject><subject>Rivers</subject><subject>Seasons</subject><subject>Seawaters, estuaries</subject><subject>Sensitivity and Specificity</subject><subject>Vibrio</subject><subject>Water analysis</subject><subject>Water Pollutants - analysis</subject><subject>Water sampling</subject><subject>Water treatment and pollution</subject><subject>Waterborne diseases</subject><subject>β-Galactosidase</subject><issn>0273-1223</issn><issn>1996-9732</issn><isbn>9781843394228</isbn><isbn>1843394227</isbn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqFkU1r3DAQhkWb0mzSHHstgtLevB2NZEs6htAvSMklObtaZdw42FIqyYT995XJQqCXnATSM6N35mHsvYAtiq778pjLFgFwC6D0K7YR1naN1RJfszOrjTBKSqsQzRHbAGrZCER5zE5yvgcALRW8Zcf1DnUHsGG_f8UwlpjG8IcPjrybuI-huHkMrowx8Djwckf8-s7NlDnlsri050teCxzPNI9uKXF2hW45uTTt-aNLYX3N-1xofsfeDG7KdHY4T9nNt6_XFz-ay6vvPy_OLxuvFJSmAxQ1udetIemMJ7JDu9tZI6ltSUuHA4KSGr3oyHuvDBix8wYcqlon5Sn7_NT3IcW_S83Zz2P2NE0uUFxyj3U5Srbdy6DAFlSrXgah052FFfz4H3gflxTqtL2wSiphNK4BmyfKp5hzoqF_SONcl9kL6Fe1fVXbr2r7VW3lPxy6LruZbp_pg7sKfDoALldvQ3LBj_mZq_9iK6T8B72KqM0</recordid><startdate>20020101</startdate><enddate>20020101</enddate><creator>TRYLAND, I</creator><creator>SURMAN, S</creator><creator>BERG, J. 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D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-6021433c758e3a8cee9f5bb983e55e73a2f204372c16eccc48081bc80a2443333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Aeromonas</topic><topic>Applied sciences</topic><topic>Automation</topic><topic>Bacteria</topic><topic>beta-Galactosidase - analysis</topic><topic>beta-Galactosidase - pharmacology</topic><topic>Biological Assay - methods</topic><topic>Brackish</topic><topic>British Isles, England, Thames R</topic><topic>Cattle</topic><topic>Coliforms</topic><topic>Contamination</topic><topic>Early warning systems</topic><topic>Earth sciences</topic><topic>Earth, ocean, space</topic><topic>Engineering and environment geology. 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D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monitoring faecal contamination of the Thames estuary using a semiautomated early warning system</atitle><jtitle>Water science and technology</jtitle><addtitle>Water Sci Technol</addtitle><date>2002-01-01</date><risdate>2002</risdate><volume>46</volume><issue>3</issue><spage>25</spage><epage>31</epage><pages>25-31</pages><issn>0273-1223</issn><eissn>1996-9732</eissn><isbn>9781843394228</isbn><isbn>1843394227</isbn><coden>WSTED4</coden><abstract>The Colifast Early Warning System, based on measuring beta-galactosidase activity (2 h method), was evaluated for monitoring the level of faecal contamination in the upper tidal Thames. Two trials were performed, one following heavy rain in November 2000, the next during a dry and sunny period in July 2001. In general the beta-galactosidase activity and the two coliform reference methods (recovery following membrane filtration with membrane lauryl sulphate broth (MLSB) and Colilert Quantitray) were comparable. However, in several samples in July the beta-galactosidase activity seemed to overestimate the number of culturable coliforms, suggesting that the rapid enzymatic method detected beta-galactosidase produced by other bacterial sources, such as Aeromonas spp. or Vibrio spp., or nonculturable coliforms. The later could be attributed to sunlight-induced injury. Nevertheless, the rapid method based on beta-galactosidase activity gave an estimate of the level of culturable coliforms, which did not differ from both coliform reference methods by more than one log. Monitoring of beta-galactosidase activity in river water samples using the Colifast Analyser may therefore be useful as an early warning indicator of faecal contamination.</abstract><cop>London</cop><pub>IWA</pub><pmid>12227600</pmid><doi>10.2166/wst.2002.0047</doi><tpages>7</tpages></addata></record> |
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| ispartof | Water science and technology, 2002-01, Vol.46 (3), p.25-31 |
| issn | 0273-1223 1996-9732 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Aeromonas Applied sciences Automation Bacteria beta-Galactosidase - analysis beta-Galactosidase - pharmacology Biological Assay - methods Brackish British Isles, England, Thames R Cattle Coliforms Contamination Early warning systems Earth sciences Earth, ocean, space Engineering and environment geology. Geothermics England Enterobacteriaceae - isolation & purification Environmental monitoring Environmental Monitoring - methods Estuaries Estuarine dynamics Exact sciences and technology Feces Feces - enzymology Galactosidase Membrane filtration Methods Natural water pollution Pollution Pollution, environment geology Rain Rainfall River water Rivers Seasons Seawaters, estuaries Sensitivity and Specificity Vibrio Water analysis Water Pollutants - analysis Water sampling Water treatment and pollution Waterborne diseases β-Galactosidase |
| title | Monitoring faecal contamination of the Thames estuary using a semiautomated early warning system |
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