beta -galactosidase assays of single-cell lysates on a microchip: a complementary method for enzymatic analysis of single cells

The use of microfluidic glass chips for continuous single-cell lysis and assay of internal beta -Galactosidase ( beta -Gal) content is described. Cells were transported single file toward a Y-shaped mixing junction at which lytic agents were introduced by suction. Flow velocities of similar to 100 a...

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Veröffentlicht in:	Proceedings of the IEEE 2004-01, Vol.92 (1), p.115-125
Hauptverfasser:	Ocvirk, G, Salimi-Moosavi, H, Szarka, R J, Arriaga, E A, Andersson, P E, Smith, R, Dovichi, N J, Harrison, D J
Format:	Artikel
Sprache:	eng
Schlagworte:	Assaying Fluorescein Fluorescence Microfluidics Optimization Proteins Sodium Triton
Online-Zugang:	Volltext
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