Inhalation of gaseous hypochlorous acid and its effect on human respiratory epithelial cells in laboratory model systems
During the disinfection of indoor spaces using gaseous hypochlorous acid (HOCl ), inhalation is the most common route of exposure for humans. In this study, an artificial human respiratory tract model was exposed to 12-140 ppb HOCl at an aspiration flow rate of 800 mL/s for 15 h in a 1 m chamber. Th...
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| Veröffentlicht in: | Journal of microorganism control 2024, Vol.29 (1), p.39-44 |
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| creator | Muramatsu, Takashi Kodama, Kazuya Yamada, Tetsuyoshi Yamada, Atsushi Fukuzaki, Satoshi |
| description | During the disinfection of indoor spaces using gaseous hypochlorous acid (HOCl
), inhalation is the most common route of exposure for humans. In this study, an artificial human respiratory tract model was exposed to 12-140 ppb HOCl
at an aspiration flow rate of 800 mL/s for 15 h in a 1 m
chamber. The respiratory tract model was equipped with 5th order bronchi and all gas-contact parts were made of silicone rubber with no other chlorine-consuming substances. The concentration of HOCl
reaching the lung pseudo-space was approximately 47.4% of the HOCl
concentrations in the chamber and was calculated to be very close to zero when the chamber concentration was less than 20.5 ppb. The disappearance of HOCl
during inhalation is likely due to the adsorption of HOCl
on the gas-contact silicone rubber surfaces. The cytotoxicity of HOCl
on respiratory epithelial cells was also examined using human air-liquid-interface airway tissue models. Human nasal epithelium and bronchiolar epithelium were exposed to 100 ppb and 500 ppb HOCl
for 8 h and 5 d, respectively. No significant effects of HOCl
on cell viability and ciliary activity were observed in any cell type, indicating that low concentrations of HOCl
, less than 500 ppb, had no cytotoxic effect. |
| doi_str_mv | 10.4265/jmc.29.1_39 |
| format | Article |
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), inhalation is the most common route of exposure for humans. In this study, an artificial human respiratory tract model was exposed to 12-140 ppb HOCl
at an aspiration flow rate of 800 mL/s for 15 h in a 1 m
chamber. The respiratory tract model was equipped with 5th order bronchi and all gas-contact parts were made of silicone rubber with no other chlorine-consuming substances. The concentration of HOCl
reaching the lung pseudo-space was approximately 47.4% of the HOCl
concentrations in the chamber and was calculated to be very close to zero when the chamber concentration was less than 20.5 ppb. The disappearance of HOCl
during inhalation is likely due to the adsorption of HOCl
on the gas-contact silicone rubber surfaces. The cytotoxicity of HOCl
on respiratory epithelial cells was also examined using human air-liquid-interface airway tissue models. Human nasal epithelium and bronchiolar epithelium were exposed to 100 ppb and 500 ppb HOCl
for 8 h and 5 d, respectively. No significant effects of HOCl
on cell viability and ciliary activity were observed in any cell type, indicating that low concentrations of HOCl
, less than 500 ppb, had no cytotoxic effect.</description><identifier>ISSN: 2758-6383</identifier><identifier>EISSN: 2758-6391</identifier><identifier>DOI: 10.4265/jmc.29.1_39</identifier><identifier>PMID: 38508761</identifier><language>eng</language><publisher>Japan</publisher><subject>Epithelial Cells ; Gases ; Humans ; Hypochlorous Acid - pharmacology ; Lung ; Silicone Elastomers</subject><ispartof>Journal of microorganism control, 2024, Vol.29 (1), p.39-44</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c333t-d38c19e8907a2e16162db073b71156d062cdda0b656318f57f69874eb2b440f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,4025,27925,27926,27927</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38508761$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Muramatsu, Takashi</creatorcontrib><creatorcontrib>Kodama, Kazuya</creatorcontrib><creatorcontrib>Yamada, Tetsuyoshi</creatorcontrib><creatorcontrib>Yamada, Atsushi</creatorcontrib><creatorcontrib>Fukuzaki, Satoshi</creatorcontrib><title>Inhalation of gaseous hypochlorous acid and its effect on human respiratory epithelial cells in laboratory model systems</title><title>Journal of microorganism control</title><addtitle>J Microorg Control</addtitle><description>During the disinfection of indoor spaces using gaseous hypochlorous acid (HOCl
), inhalation is the most common route of exposure for humans. In this study, an artificial human respiratory tract model was exposed to 12-140 ppb HOCl
at an aspiration flow rate of 800 mL/s for 15 h in a 1 m
chamber. The respiratory tract model was equipped with 5th order bronchi and all gas-contact parts were made of silicone rubber with no other chlorine-consuming substances. The concentration of HOCl
reaching the lung pseudo-space was approximately 47.4% of the HOCl
concentrations in the chamber and was calculated to be very close to zero when the chamber concentration was less than 20.5 ppb. The disappearance of HOCl
during inhalation is likely due to the adsorption of HOCl
on the gas-contact silicone rubber surfaces. The cytotoxicity of HOCl
on respiratory epithelial cells was also examined using human air-liquid-interface airway tissue models. Human nasal epithelium and bronchiolar epithelium were exposed to 100 ppb and 500 ppb HOCl
for 8 h and 5 d, respectively. No significant effects of HOCl
on cell viability and ciliary activity were observed in any cell type, indicating that low concentrations of HOCl
, less than 500 ppb, had no cytotoxic effect.</description><subject>Epithelial Cells</subject><subject>Gases</subject><subject>Humans</subject><subject>Hypochlorous Acid - pharmacology</subject><subject>Lung</subject><subject>Silicone Elastomers</subject><issn>2758-6383</issn><issn>2758-6391</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtrwzAMgM3YWEfX0-7Dx8Fo50diO8dR9igUduk9OLazuDhxZjuw_PsltOtJEvokpA-AB4w2GWH5y7FVG1JscEmLK3BHeC7WjBb4-pILugCrGI8IIUpRJmh2CxZU5Ehwhu_A765rpJPJ-g76Gn7LaPwQYTP2XjXOh7mQymooOw1titDUtVEJTngztLKDwcTeBpl8GKHpbWqMs9JBZZyL0HbQycqf263XxsE4xmTaeA9uaumiWZ3jEhze3w7bz_X-62O3fd2vFaU0rTUVChdGFIhLYjDDjOgKcVpxjHOmESNKa4kqljOKRZ3zmhWCZ6YiVZahmi7B02ltH_zPYGIqWxvn42Q3P1qSglOMMjJ5WoLnE6qCjzGYuuyDbWUYS4zKWXY5yZ4Gyln2RD-eFw9Va_SF_VdL_wAxfHxq</recordid><startdate>2024</startdate><enddate>2024</enddate><creator>Muramatsu, Takashi</creator><creator>Kodama, Kazuya</creator><creator>Yamada, Tetsuyoshi</creator><creator>Yamada, Atsushi</creator><creator>Fukuzaki, Satoshi</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2024</creationdate><title>Inhalation of gaseous hypochlorous acid and its effect on human respiratory epithelial cells in laboratory model systems</title><author>Muramatsu, Takashi ; Kodama, Kazuya ; Yamada, Tetsuyoshi ; Yamada, Atsushi ; Fukuzaki, Satoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c333t-d38c19e8907a2e16162db073b71156d062cdda0b656318f57f69874eb2b440f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Epithelial Cells</topic><topic>Gases</topic><topic>Humans</topic><topic>Hypochlorous Acid - pharmacology</topic><topic>Lung</topic><topic>Silicone Elastomers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Muramatsu, Takashi</creatorcontrib><creatorcontrib>Kodama, Kazuya</creatorcontrib><creatorcontrib>Yamada, Tetsuyoshi</creatorcontrib><creatorcontrib>Yamada, Atsushi</creatorcontrib><creatorcontrib>Fukuzaki, Satoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microorganism control</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muramatsu, Takashi</au><au>Kodama, Kazuya</au><au>Yamada, Tetsuyoshi</au><au>Yamada, Atsushi</au><au>Fukuzaki, Satoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhalation of gaseous hypochlorous acid and its effect on human respiratory epithelial cells in laboratory model systems</atitle><jtitle>Journal of microorganism control</jtitle><addtitle>J Microorg Control</addtitle><date>2024</date><risdate>2024</risdate><volume>29</volume><issue>1</issue><spage>39</spage><epage>44</epage><pages>39-44</pages><issn>2758-6383</issn><eissn>2758-6391</eissn><abstract>During the disinfection of indoor spaces using gaseous hypochlorous acid (HOCl
), inhalation is the most common route of exposure for humans. In this study, an artificial human respiratory tract model was exposed to 12-140 ppb HOCl
at an aspiration flow rate of 800 mL/s for 15 h in a 1 m
chamber. The respiratory tract model was equipped with 5th order bronchi and all gas-contact parts were made of silicone rubber with no other chlorine-consuming substances. The concentration of HOCl
reaching the lung pseudo-space was approximately 47.4% of the HOCl
concentrations in the chamber and was calculated to be very close to zero when the chamber concentration was less than 20.5 ppb. The disappearance of HOCl
during inhalation is likely due to the adsorption of HOCl
on the gas-contact silicone rubber surfaces. The cytotoxicity of HOCl
on respiratory epithelial cells was also examined using human air-liquid-interface airway tissue models. Human nasal epithelium and bronchiolar epithelium were exposed to 100 ppb and 500 ppb HOCl
for 8 h and 5 d, respectively. No significant effects of HOCl
on cell viability and ciliary activity were observed in any cell type, indicating that low concentrations of HOCl
, less than 500 ppb, had no cytotoxic effect.</abstract><cop>Japan</cop><pmid>38508761</pmid><doi>10.4265/jmc.29.1_39</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 2758-6383 2758-6391 |
| language | eng |
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| source | MEDLINE; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese |
| subjects | Epithelial Cells Gases Humans Hypochlorous Acid - pharmacology Lung Silicone Elastomers |
| title | Inhalation of gaseous hypochlorous acid and its effect on human respiratory epithelial cells in laboratory model systems |
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