potential of confocal imaging for measuring physiological changes in brewer's yeast
ABSTRACT This study focused on the implementation of fluorescence optical methods and laser scanning confocal microscopy for monitoring brewing yeast performance. Physiological parameters and cell compounds in yeast cells (glycogen, neutral lipids, trehalose, bud scars, DNA and intracellular protein...
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| Veröffentlicht in: | Journal of the Institute of Brewing 2006, Vol.112 (2), p.134-147 |
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| container_end_page | 147 |
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| container_issue | 2 |
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| container_title | Journal of the Institute of Brewing |
| container_volume | 112 |
| creator | Schlee, C Miedl, M Leiper, K.A Stewart, G.G |
| description | ABSTRACT
This study focused on the implementation of fluorescence optical methods and laser scanning confocal microscopy for monitoring brewing yeast performance. Physiological parameters and cell compounds in yeast cells (glycogen, neutral lipids, trehalose, bud scars, DNA and intracellular proteinases) have been successfully visualised with the aid of highly specific fluorochromes. The expression and sub cellular localisation of proteinase A during fermentation has been studied employing a Saccharomyces cerevisiae green fluorescent protein clone. This novel approach to monitoring brewing yeast performance provides new insights into physiological events that occur during wort fermentation. |
| doi_str_mv | 10.1002/j.2050-0416.2006.tb00243.x |
| format | Article |
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This study focused on the implementation of fluorescence optical methods and laser scanning confocal microscopy for monitoring brewing yeast performance. Physiological parameters and cell compounds in yeast cells (glycogen, neutral lipids, trehalose, bud scars, DNA and intracellular proteinases) have been successfully visualised with the aid of highly specific fluorochromes. The expression and sub cellular localisation of proteinase A during fermentation has been studied employing a Saccharomyces cerevisiae green fluorescent protein clone. This novel approach to monitoring brewing yeast performance provides new insights into physiological events that occur during wort fermentation.</description><identifier>ISSN: 0046-9750</identifier><identifier>EISSN: 2050-0416</identifier><identifier>DOI: 10.1002/j.2050-0416.2006.tb00243.x</identifier><identifier>CODEN: JINBAL</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Beers ; Biological and medical sciences ; brewers yeast ; Brewing ; chemical constituents of plants ; confocal imaging ; confocal scanning laser microscopy ; fermentation ; Fermented food industries ; fluorescence emission spectroscopy ; fluorescence microscopy ; Food industries ; Fundamental and applied biological sciences. Psychology ; fungal anatomy ; glycogen ; green fluorescent protein ; lipid content ; proteinase ; proteinases ; quantitative analysis ; Saccharomyces cerevisiae ; trehalose ; yeast</subject><ispartof>Journal of the Institute of Brewing, 2006, Vol.112 (2), p.134-147</ispartof><rights>2006 The Institute of Brewing & Distilling</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4933-d00eff8c7973b4c6afddcf389edffecfb079d8d5ff716bb9133e310c1745e8db3</citedby><cites>FETCH-LOGICAL-c4933-d00eff8c7973b4c6afddcf389edffecfb079d8d5ff716bb9133e310c1745e8db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fj.2050-0416.2006.tb00243.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fj.2050-0416.2006.tb00243.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,4021,27921,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18281638$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Schlee, C</creatorcontrib><creatorcontrib>Miedl, M</creatorcontrib><creatorcontrib>Leiper, K.A</creatorcontrib><creatorcontrib>Stewart, G.G</creatorcontrib><title>potential of confocal imaging for measuring physiological changes in brewer's yeast</title><title>Journal of the Institute of Brewing</title><description>ABSTRACT
This study focused on the implementation of fluorescence optical methods and laser scanning confocal microscopy for monitoring brewing yeast performance. Physiological parameters and cell compounds in yeast cells (glycogen, neutral lipids, trehalose, bud scars, DNA and intracellular proteinases) have been successfully visualised with the aid of highly specific fluorochromes. The expression and sub cellular localisation of proteinase A during fermentation has been studied employing a Saccharomyces cerevisiae green fluorescent protein clone. This novel approach to monitoring brewing yeast performance provides new insights into physiological events that occur during wort fermentation.</description><subject>Beers</subject><subject>Biological and medical sciences</subject><subject>brewers yeast</subject><subject>Brewing</subject><subject>chemical constituents of plants</subject><subject>confocal imaging</subject><subject>confocal scanning laser microscopy</subject><subject>fermentation</subject><subject>Fermented food industries</subject><subject>fluorescence emission spectroscopy</subject><subject>fluorescence microscopy</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>fungal anatomy</subject><subject>glycogen</subject><subject>green fluorescent protein</subject><subject>lipid content</subject><subject>proteinase</subject><subject>proteinases</subject><subject>quantitative analysis</subject><subject>Saccharomyces cerevisiae</subject><subject>trehalose</subject><subject>yeast</subject><issn>0046-9750</issn><issn>2050-0416</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqVkEFP3DAQhS3USmyhv6EREu0pyzi2Y4cDUotaSgXtYUFwsxzHDl6y8dbOit1_X0dZ0TMnP48_v5l5CJ1gmGOA4mw5L4BBDhSXSUE5H-pUpmS-PUCz16d3aAZAy7ziDA7RhxiXAIwWmM3QYu0H0w9OdZm3mfa99Tppt1Kt69vM-pCtjIqbMN7WT7vofOdbNzL6SfWtiZnrszqYFxO-xGyX2OEYvbeqi-bj_jxC9z--313-zG_-XF1ffr3JNa0IyRsAY63QvOKkprpUtmm0JaIyjbVG2xp41YiGWctxWdcVJsQQDBpzyoxoanKEPk--6-D_bkwc5MpFbbpO9cZvoiwqDlywMoHnE6iDjzEYK9chbRh2EoMcc5RLOYYlx7DkmKPc5yi36fPpvouKaW0bVK9d_O8gCoFLIhJ3MXEvrjO7N3SQv66_JZEM8snAxcFsXw1UeJYlJ5zJh99XksDDHbvlj3KR-E8Tb5WXqg1pqPtFAZgAhkpQTMk_Slyhrg</recordid><startdate>2006</startdate><enddate>2006</enddate><creator>Schlee, C</creator><creator>Miedl, M</creator><creator>Leiper, K.A</creator><creator>Stewart, G.G</creator><general>Blackwell Publishing Ltd</general><general>Institute of Brewing</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope></search><sort><creationdate>2006</creationdate><title>potential of confocal imaging for measuring physiological changes in brewer's yeast</title><author>Schlee, C ; Miedl, M ; Leiper, K.A ; Stewart, G.G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4933-d00eff8c7973b4c6afddcf389edffecfb079d8d5ff716bb9133e310c1745e8db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Beers</topic><topic>Biological and medical sciences</topic><topic>brewers yeast</topic><topic>Brewing</topic><topic>chemical constituents of plants</topic><topic>confocal imaging</topic><topic>confocal scanning laser microscopy</topic><topic>fermentation</topic><topic>Fermented food industries</topic><topic>fluorescence emission spectroscopy</topic><topic>fluorescence microscopy</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>fungal anatomy</topic><topic>glycogen</topic><topic>green fluorescent protein</topic><topic>lipid content</topic><topic>proteinase</topic><topic>proteinases</topic><topic>quantitative analysis</topic><topic>Saccharomyces cerevisiae</topic><topic>trehalose</topic><topic>yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schlee, C</creatorcontrib><creatorcontrib>Miedl, M</creatorcontrib><creatorcontrib>Leiper, K.A</creatorcontrib><creatorcontrib>Stewart, G.G</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><jtitle>Journal of the Institute of Brewing</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schlee, C</au><au>Miedl, M</au><au>Leiper, K.A</au><au>Stewart, G.G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>potential of confocal imaging for measuring physiological changes in brewer's yeast</atitle><jtitle>Journal of the Institute of Brewing</jtitle><date>2006</date><risdate>2006</risdate><volume>112</volume><issue>2</issue><spage>134</spage><epage>147</epage><pages>134-147</pages><issn>0046-9750</issn><eissn>2050-0416</eissn><coden>JINBAL</coden><abstract>ABSTRACT
This study focused on the implementation of fluorescence optical methods and laser scanning confocal microscopy for monitoring brewing yeast performance. Physiological parameters and cell compounds in yeast cells (glycogen, neutral lipids, trehalose, bud scars, DNA and intracellular proteinases) have been successfully visualised with the aid of highly specific fluorochromes. The expression and sub cellular localisation of proteinase A during fermentation has been studied employing a Saccharomyces cerevisiae green fluorescent protein clone. This novel approach to monitoring brewing yeast performance provides new insights into physiological events that occur during wort fermentation.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1002/j.2050-0416.2006.tb00243.x</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of the Institute of Brewing, 2006, Vol.112 (2), p.134-147 |
| issn | 0046-9750 2050-0416 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_29707856 |
| source | Wiley Online Library - AutoHoldings Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Beers Biological and medical sciences brewers yeast Brewing chemical constituents of plants confocal imaging confocal scanning laser microscopy fermentation Fermented food industries fluorescence emission spectroscopy fluorescence microscopy Food industries Fundamental and applied biological sciences. Psychology fungal anatomy glycogen green fluorescent protein lipid content proteinase proteinases quantitative analysis Saccharomyces cerevisiae trehalose yeast |
| title | potential of confocal imaging for measuring physiological changes in brewer's yeast |
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