Cell‑free fetal DNA at 11‑13 weeks of gestation is not altered in complicated pregnancies
Non-invasive maternal cell-free fetal DNA (cffDNA) is a promising biomarker for screening common genetic syndromes. Alterations in the expression levels of cffDNA in the maternal circulation have been demonstrated in abnormal pregnancies. However, the results are conflicting. The present study aimed...
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| Veröffentlicht in: | Biomedical reports 2024-04, Vol.20 (4), p.69-69, Article 69 |
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| creator | Koukou, Zoi Panteris, Eleftherios Manolakos, Emmanouel Papadopoulos, Aristeidis Papoulidis, Ioannis Relakis, Konstantinos Sifakis, Stavros |
| description | Non-invasive maternal cell-free fetal DNA (cffDNA) is a promising biomarker for screening common genetic syndromes. Alterations in the expression levels of cffDNA in the maternal circulation have been demonstrated in abnormal pregnancies. However, the results are conflicting. The present study aimed to investigate whether cffDNA levels are associated with pregnancy complications. The study group comprised pregnant women who presented with pregnancy complications, such as preterm birth, gestational hypertension, intrauterine growth retardation, gestational diabetes, polyhydramnios, oligohydramnios, vaginal bleeding and placental abruption. The control group comprised women who had a normal pregnancy course. Blood samples were obtained from 500 pregnant women between 11-13 weeks of gestation. cffDNA was amplified, sequenced and analyzed using the next-generation aneuploidy test of a Panorama-Natera kit. Nuchal translucency (NT) thickness as well as pregnancy associated plasma protein-A (PAPP-A) and β-human chorionic gonadotropin (β-hCG) levels were also assessed. Statistical analysis was performed in 494 out of the 500 samples collected with SPSS v.26 using non-parametric methods. The parameters were normalized by the multiples of median (MoM) method. The expression levels of PAPP-A, β-hCG, and the NT mean MoM values were significantly different between the study and control groups (P=0.005, P |
| doi_str_mv | 10.3892/br.2024.1757 |
| format | Article |
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Alterations in the expression levels of cffDNA in the maternal circulation have been demonstrated in abnormal pregnancies. However, the results are conflicting. The present study aimed to investigate whether cffDNA levels are associated with pregnancy complications. The study group comprised pregnant women who presented with pregnancy complications, such as preterm birth, gestational hypertension, intrauterine growth retardation, gestational diabetes, polyhydramnios, oligohydramnios, vaginal bleeding and placental abruption. The control group comprised women who had a normal pregnancy course. Blood samples were obtained from 500 pregnant women between 11-13 weeks of gestation. cffDNA was amplified, sequenced and analyzed using the next-generation aneuploidy test of a Panorama-Natera kit. Nuchal translucency (NT) thickness as well as pregnancy associated plasma protein-A (PAPP-A) and β-human chorionic gonadotropin (β-hCG) levels were also assessed. Statistical analysis was performed in 494 out of the 500 samples collected with SPSS v.26 using non-parametric methods. The parameters were normalized by the multiples of median (MoM) method. The expression levels of PAPP-A, β-hCG, and the NT mean MoM values were significantly different between the study and control groups (P=0.005, P<0.001 and P=0.007, respectively). However, the expression levels of cffDNA and the mean MoM values were not significantly different between these two groups (P=0.687). The findings of the present study support the conclusion that cffDNA expression is not altered in a series of pregnancy complications. The prognostic value of cffDNA in predicting adverse pregnancy outcomes requires further investigation.</description><identifier>ISSN: 2049-9434</identifier><identifier>EISSN: 2049-9442</identifier><identifier>DOI: 10.3892/br.2024.1757</identifier><identifier>PMID: 38495346</identifier><language>eng</language><publisher>England: Spandidos Publications</publisher><subject>Amniotic fluid ; Analysis ; Aneuploidy ; Biomarkers ; Chorionic gonadotropin ; Demographics ; Deoxyribonucleic acid ; Diabetes mellitus ; Diabetes therapy ; DNA ; Eclampsia ; Ethylenediaminetetraacetic acid ; Fetuses ; Gestation ; Gestational diabetes ; Gonadotropins ; Growth rate ; Gynecology ; Hospitals ; Hypertension ; Infants (Premature) ; Maximum likelihood method ; Mean ; Medical screening ; Miscarriage ; Newborn babies ; Obstetrics ; Pituitary (anterior) ; Placenta ; Preeclampsia ; Pregnancy ; Pregnancy complications ; Pregnant women ; Premature birth ; Statistical analysis ; Vagina ; Womens health</subject><ispartof>Biomedical reports, 2024-04, Vol.20 (4), p.69-69, Article 69</ispartof><rights>Copyright: © 2024 Koukou et al.</rights><rights>COPYRIGHT 2024 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2024</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c381t-6ac746308e5fe2b5525a4f58d118dfa73b6b07d4530f6f8cff7af70e6987b43f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38495346$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koukou, Zoi</creatorcontrib><creatorcontrib>Panteris, Eleftherios</creatorcontrib><creatorcontrib>Manolakos, Emmanouel</creatorcontrib><creatorcontrib>Papadopoulos, Aristeidis</creatorcontrib><creatorcontrib>Papoulidis, Ioannis</creatorcontrib><creatorcontrib>Relakis, Konstantinos</creatorcontrib><creatorcontrib>Sifakis, Stavros</creatorcontrib><title>Cell‑free fetal DNA at 11‑13 weeks of gestation is not altered in complicated pregnancies</title><title>Biomedical reports</title><addtitle>Biomed Rep</addtitle><description>Non-invasive maternal cell-free fetal DNA (cffDNA) is a promising biomarker for screening common genetic syndromes. Alterations in the expression levels of cffDNA in the maternal circulation have been demonstrated in abnormal pregnancies. However, the results are conflicting. The present study aimed to investigate whether cffDNA levels are associated with pregnancy complications. The study group comprised pregnant women who presented with pregnancy complications, such as preterm birth, gestational hypertension, intrauterine growth retardation, gestational diabetes, polyhydramnios, oligohydramnios, vaginal bleeding and placental abruption. The control group comprised women who had a normal pregnancy course. Blood samples were obtained from 500 pregnant women between 11-13 weeks of gestation. cffDNA was amplified, sequenced and analyzed using the next-generation aneuploidy test of a Panorama-Natera kit. Nuchal translucency (NT) thickness as well as pregnancy associated plasma protein-A (PAPP-A) and β-human chorionic gonadotropin (β-hCG) levels were also assessed. Statistical analysis was performed in 494 out of the 500 samples collected with SPSS v.26 using non-parametric methods. The parameters were normalized by the multiples of median (MoM) method. The expression levels of PAPP-A, β-hCG, and the NT mean MoM values were significantly different between the study and control groups (P=0.005, P<0.001 and P=0.007, respectively). However, the expression levels of cffDNA and the mean MoM values were not significantly different between these two groups (P=0.687). The findings of the present study support the conclusion that cffDNA expression is not altered in a series of pregnancy complications. The prognostic value of cffDNA in predicting adverse pregnancy outcomes requires further investigation.</description><subject>Amniotic fluid</subject><subject>Analysis</subject><subject>Aneuploidy</subject><subject>Biomarkers</subject><subject>Chorionic gonadotropin</subject><subject>Demographics</subject><subject>Deoxyribonucleic acid</subject><subject>Diabetes mellitus</subject><subject>Diabetes therapy</subject><subject>DNA</subject><subject>Eclampsia</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Fetuses</subject><subject>Gestation</subject><subject>Gestational diabetes</subject><subject>Gonadotropins</subject><subject>Growth rate</subject><subject>Gynecology</subject><subject>Hospitals</subject><subject>Hypertension</subject><subject>Infants (Premature)</subject><subject>Maximum likelihood method</subject><subject>Mean</subject><subject>Medical screening</subject><subject>Miscarriage</subject><subject>Newborn babies</subject><subject>Obstetrics</subject><subject>Pituitary (anterior)</subject><subject>Placenta</subject><subject>Preeclampsia</subject><subject>Pregnancy</subject><subject>Pregnancy complications</subject><subject>Pregnant women</subject><subject>Premature birth</subject><subject>Statistical analysis</subject><subject>Vagina</subject><subject>Womens health</subject><issn>2049-9434</issn><issn>2049-9442</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNptkU1uFDEQhS0EIlHIjjWyxIYFM_i3bS9Hw68UwQbWlttdHjl024PtEWLHFbhBzpKjcBI8SggCYS9cLn2v9EoPoceUrLk27MVY1owwsaZKqnvolBFhVkYIdv-u5uIEndd6SfoxijCpH6ITroWRXAynyG5hnn9-_xEKAA7Q3Ixfvt9g166vKO19yq-vvgJ8rjgHvIPaXIs54Vhxyg27uUGBCceEfV72c_Su9e--wC655CPUR-hBcHOF89v3DH16_erj9u3q4sObd9vNxcpzTdtqcF6JgRMNMgAbpWTSiSD1RKmeglN8HEaiJiE5CUPQPgTlgiIwGK1GwQM_Q89u5u5L_nLoPu0Sq--7uQT5UC0zgyJSaSY6-vQf9DIfSuruLCeEC6I45X-onZvBxhRyK84fh9qNMoxLbZTu1Po_VL8TLNHnBCH2_l-C5zcCX3KtBYLdl7i48s1SYo-R2rHYY6T2GGnHn9x6PYwLTHfw7wD5L_GKm_U</recordid><startdate>20240401</startdate><enddate>20240401</enddate><creator>Koukou, Zoi</creator><creator>Panteris, Eleftherios</creator><creator>Manolakos, Emmanouel</creator><creator>Papadopoulos, Aristeidis</creator><creator>Papoulidis, Ioannis</creator><creator>Relakis, Konstantinos</creator><creator>Sifakis, Stavros</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20240401</creationdate><title>Cell‑free fetal DNA at 11‑13 weeks of gestation is not altered in complicated pregnancies</title><author>Koukou, Zoi ; 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Alterations in the expression levels of cffDNA in the maternal circulation have been demonstrated in abnormal pregnancies. However, the results are conflicting. The present study aimed to investigate whether cffDNA levels are associated with pregnancy complications. The study group comprised pregnant women who presented with pregnancy complications, such as preterm birth, gestational hypertension, intrauterine growth retardation, gestational diabetes, polyhydramnios, oligohydramnios, vaginal bleeding and placental abruption. The control group comprised women who had a normal pregnancy course. Blood samples were obtained from 500 pregnant women between 11-13 weeks of gestation. cffDNA was amplified, sequenced and analyzed using the next-generation aneuploidy test of a Panorama-Natera kit. Nuchal translucency (NT) thickness as well as pregnancy associated plasma protein-A (PAPP-A) and β-human chorionic gonadotropin (β-hCG) levels were also assessed. Statistical analysis was performed in 494 out of the 500 samples collected with SPSS v.26 using non-parametric methods. The parameters were normalized by the multiples of median (MoM) method. The expression levels of PAPP-A, β-hCG, and the NT mean MoM values were significantly different between the study and control groups (P=0.005, P<0.001 and P=0.007, respectively). However, the expression levels of cffDNA and the mean MoM values were not significantly different between these two groups (P=0.687). The findings of the present study support the conclusion that cffDNA expression is not altered in a series of pregnancy complications. The prognostic value of cffDNA in predicting adverse pregnancy outcomes requires further investigation.</abstract><cop>England</cop><pub>Spandidos Publications</pub><pmid>38495346</pmid><doi>10.3892/br.2024.1757</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | Spandidos Publications Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Amniotic fluid Analysis Aneuploidy Biomarkers Chorionic gonadotropin Demographics Deoxyribonucleic acid Diabetes mellitus Diabetes therapy DNA Eclampsia Ethylenediaminetetraacetic acid Fetuses Gestation Gestational diabetes Gonadotropins Growth rate Gynecology Hospitals Hypertension Infants (Premature) Maximum likelihood method Mean Medical screening Miscarriage Newborn babies Obstetrics Pituitary (anterior) Placenta Preeclampsia Pregnancy Pregnancy complications Pregnant women Premature birth Statistical analysis Vagina Womens health |
| title | Cell‑free fetal DNA at 11‑13 weeks of gestation is not altered in complicated pregnancies |
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