Effect of Woven Fabric Biodegradable Polyglycolic Acid on Joint Resurfacing with Fresh Chondrocytes in a Rabbit Model
To evaluate the effectiveness of the cell-material in situ on joint resurfacing, a woven fabric polyglycolic acid (PGA) treated with fresh chondrocytes was used for repairing cartilage defects. Full-thickness defects were created in the weight-bearing surfaces of the femoral intercondylar fossa in a...
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Veröffentlicht in: | Key engineering materials 2007-01, Vol.330-332, p.1197-1200 |
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creator | Yan, Shi Gui Guan, Bing Gang Shi, Zhong Li Liu, Yang Bo Feng, Jie Yan, Wei Qi |
description | To evaluate the effectiveness of the cell-material in situ on joint resurfacing, a woven
fabric polyglycolic acid (PGA) treated with fresh chondrocytes was used for repairing cartilage
defects. Full-thickness defects were created in the weight-bearing surfaces of the femoral
intercondylar fossa in a rabbit model. The defect was filled with and without PGA under surgical
condition. Before implantation, chondrocytes were co-cultured with PGA for one day. The animals
were sacrificed at eight weeks after implantation and evaluated grossly and histological score.
Morphological examination showed that for PGA/chondrocytes group, the repaired tissue appeared
similar in color and texture to the surrounding articular surface. While for the untreated control, no
cartilage-like tissue was observed at all defects, but connective fibrous tissue. Histological analysis
revealed neochondrogenesis and clusters of cartilage matrix with specific safranin-O staining for the
PGA/cell group. The Gross and histological evaluation indicated a significantly higher score for
PGA/cell group than for PGA and control group. These results suggest that the woven fabric PGA
may facilitate the formation of cartilage tissues by providing a biodegradable and good-handle
vehicle for the delivery to and retention of organized cell matrix constructs in vivo site. It might
therefore enhance neochondrogenesis because of the superior biodegradable and biocompatible of
PGA scaffold sheet, while the more suitable biological environment might sustain cell growth and
in situ cell function, suggesting a promising candidate for functional tissue engineering of clinical
environment. |
doi_str_mv | 10.4028/www.scientific.net/KEM.330-332.1197 |
format | Article |
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fabric polyglycolic acid (PGA) treated with fresh chondrocytes was used for repairing cartilage
defects. Full-thickness defects were created in the weight-bearing surfaces of the femoral
intercondylar fossa in a rabbit model. The defect was filled with and without PGA under surgical
condition. Before implantation, chondrocytes were co-cultured with PGA for one day. The animals
were sacrificed at eight weeks after implantation and evaluated grossly and histological score.
Morphological examination showed that for PGA/chondrocytes group, the repaired tissue appeared
similar in color and texture to the surrounding articular surface. While for the untreated control, no
cartilage-like tissue was observed at all defects, but connective fibrous tissue. Histological analysis
revealed neochondrogenesis and clusters of cartilage matrix with specific safranin-O staining for the
PGA/cell group. The Gross and histological evaluation indicated a significantly higher score for
PGA/cell group than for PGA and control group. These results suggest that the woven fabric PGA
may facilitate the formation of cartilage tissues by providing a biodegradable and good-handle
vehicle for the delivery to and retention of organized cell matrix constructs in vivo site. It might
therefore enhance neochondrogenesis because of the superior biodegradable and biocompatible of
PGA scaffold sheet, while the more suitable biological environment might sustain cell growth and
in situ cell function, suggesting a promising candidate for functional tissue engineering of clinical
environment.</description><identifier>ISSN: 1013-9826</identifier><identifier>ISSN: 1662-9795</identifier><identifier>EISSN: 1662-9795</identifier><identifier>DOI: 10.4028/www.scientific.net/KEM.330-332.1197</identifier><language>eng</language><publisher>Trans Tech Publications Ltd</publisher><ispartof>Key engineering materials, 2007-01, Vol.330-332, p.1197-1200</ispartof><rights>2007 Trans Tech Publications Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c308t-3e0094865210e2268bd2617dc699af95e412f0ccfc64f390f378e6328590e0c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttps://www.scientific.net/Image/TitleCover/52?width=600</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Yan, Shi Gui</creatorcontrib><creatorcontrib>Guan, Bing Gang</creatorcontrib><creatorcontrib>Shi, Zhong Li</creatorcontrib><creatorcontrib>Liu, Yang Bo</creatorcontrib><creatorcontrib>Feng, Jie</creatorcontrib><creatorcontrib>Yan, Wei Qi</creatorcontrib><title>Effect of Woven Fabric Biodegradable Polyglycolic Acid on Joint Resurfacing with Fresh Chondrocytes in a Rabbit Model</title><title>Key engineering materials</title><description>To evaluate the effectiveness of the cell-material in situ on joint resurfacing, a woven
fabric polyglycolic acid (PGA) treated with fresh chondrocytes was used for repairing cartilage
defects. Full-thickness defects were created in the weight-bearing surfaces of the femoral
intercondylar fossa in a rabbit model. The defect was filled with and without PGA under surgical
condition. Before implantation, chondrocytes were co-cultured with PGA for one day. The animals
were sacrificed at eight weeks after implantation and evaluated grossly and histological score.
Morphological examination showed that for PGA/chondrocytes group, the repaired tissue appeared
similar in color and texture to the surrounding articular surface. While for the untreated control, no
cartilage-like tissue was observed at all defects, but connective fibrous tissue. Histological analysis
revealed neochondrogenesis and clusters of cartilage matrix with specific safranin-O staining for the
PGA/cell group. The Gross and histological evaluation indicated a significantly higher score for
PGA/cell group than for PGA and control group. These results suggest that the woven fabric PGA
may facilitate the formation of cartilage tissues by providing a biodegradable and good-handle
vehicle for the delivery to and retention of organized cell matrix constructs in vivo site. It might
therefore enhance neochondrogenesis because of the superior biodegradable and biocompatible of
PGA scaffold sheet, while the more suitable biological environment might sustain cell growth and
in situ cell function, suggesting a promising candidate for functional tissue engineering of clinical
environment.</description><issn>1013-9826</issn><issn>1662-9795</issn><issn>1662-9795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqVkE1r3DAQhk1poGmS_6BTL8WOPtaydUyW3eaTltDQo5DHo10FR0okucv--yhsIecehhl4Xx6Yp6q-M9osKO_Pd7tdk8Chz846aDzm89vVfSMErYXgDWOq-1QdMyl5rTrVfi43ZaJWPZdfqq8pPVEqWM_a42peWYuQSbDkT_iLnqzNEB2QSxdG3EQzmmFC8itM-820hzCV6ALcSIInN8H5TB4wzdEacH5Ddi5vyTpi2pLlNvgxBthnTMR5YsiDGQaXyX3hTqfVkTVTwrN_-6R6XK9-L6_qu58_rpcXdzUI2udaIKVq0cuWM4qcy34YuWTdCFIpY1WLC8YtBbAgF1YoakXXoxS8bxVFClKcVN8O3JcYXmdMWT-7BDhNxmOYk-ZKtl0neSkuD0WIIaWIVr9E92ziXjOq35Xrolx_KNdFuS7KdVFehut35YWyOlByND5lhK1-CnP05cX_4rwBCYOVDQ</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>Yan, Shi Gui</creator><creator>Guan, Bing Gang</creator><creator>Shi, Zhong Li</creator><creator>Liu, Yang Bo</creator><creator>Feng, Jie</creator><creator>Yan, Wei Qi</creator><general>Trans Tech Publications Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope></search><sort><creationdate>20070101</creationdate><title>Effect of Woven Fabric Biodegradable Polyglycolic Acid on Joint Resurfacing with Fresh Chondrocytes in a Rabbit Model</title><author>Yan, Shi Gui ; Guan, Bing Gang ; Shi, Zhong Li ; Liu, Yang Bo ; Feng, Jie ; Yan, Wei Qi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c308t-3e0094865210e2268bd2617dc699af95e412f0ccfc64f390f378e6328590e0c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yan, Shi Gui</creatorcontrib><creatorcontrib>Guan, Bing Gang</creatorcontrib><creatorcontrib>Shi, Zhong Li</creatorcontrib><creatorcontrib>Liu, Yang Bo</creatorcontrib><creatorcontrib>Feng, Jie</creatorcontrib><creatorcontrib>Yan, Wei Qi</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Key engineering materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yan, Shi Gui</au><au>Guan, Bing Gang</au><au>Shi, Zhong Li</au><au>Liu, Yang Bo</au><au>Feng, Jie</au><au>Yan, Wei Qi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Woven Fabric Biodegradable Polyglycolic Acid on Joint Resurfacing with Fresh Chondrocytes in a Rabbit Model</atitle><jtitle>Key engineering materials</jtitle><date>2007-01-01</date><risdate>2007</risdate><volume>330-332</volume><spage>1197</spage><epage>1200</epage><pages>1197-1200</pages><issn>1013-9826</issn><issn>1662-9795</issn><eissn>1662-9795</eissn><abstract>To evaluate the effectiveness of the cell-material in situ on joint resurfacing, a woven
fabric polyglycolic acid (PGA) treated with fresh chondrocytes was used for repairing cartilage
defects. Full-thickness defects were created in the weight-bearing surfaces of the femoral
intercondylar fossa in a rabbit model. The defect was filled with and without PGA under surgical
condition. Before implantation, chondrocytes were co-cultured with PGA for one day. The animals
were sacrificed at eight weeks after implantation and evaluated grossly and histological score.
Morphological examination showed that for PGA/chondrocytes group, the repaired tissue appeared
similar in color and texture to the surrounding articular surface. While for the untreated control, no
cartilage-like tissue was observed at all defects, but connective fibrous tissue. Histological analysis
revealed neochondrogenesis and clusters of cartilage matrix with specific safranin-O staining for the
PGA/cell group. The Gross and histological evaluation indicated a significantly higher score for
PGA/cell group than for PGA and control group. These results suggest that the woven fabric PGA
may facilitate the formation of cartilage tissues by providing a biodegradable and good-handle
vehicle for the delivery to and retention of organized cell matrix constructs in vivo site. It might
therefore enhance neochondrogenesis because of the superior biodegradable and biocompatible of
PGA scaffold sheet, while the more suitable biological environment might sustain cell growth and
in situ cell function, suggesting a promising candidate for functional tissue engineering of clinical
environment.</abstract><pub>Trans Tech Publications Ltd</pub><doi>10.4028/www.scientific.net/KEM.330-332.1197</doi><tpages>4</tpages></addata></record> |
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title | Effect of Woven Fabric Biodegradable Polyglycolic Acid on Joint Resurfacing with Fresh Chondrocytes in a Rabbit Model |
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