Cell-free synthesis of the Salmonella specific broad host range bacteriophage, felixO1
Phage-based biocontrol of foodborne Salmonella is limited by the requisite use of Salmonella to propagate the phages. This limitation can be circumvented by producing Salmonella phages using a cell-free gene expression system (CFE) with a non-pathogenic chassis. Here, we produce the Salmonella phage...
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| Veröffentlicht in: | Journal of microbiological methods 2024-05, Vol.220, p.106920, Article 106920 |
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| container_title | Journal of microbiological methods |
| container_volume | 220 |
| creator | McFarlane, John A. Garenne, David Noireaux, Vincent Bowden, Steven D. |
| description | Phage-based biocontrol of foodborne Salmonella is limited by the requisite use of Salmonella to propagate the phages. This limitation can be circumvented by producing Salmonella phages using a cell-free gene expression system (CFE) with a non-pathogenic chassis. Here, we produce the Salmonella phage felixO1 using an E. coli-based CFE system.
•Simple method for obtaining high-concentration phage genomic DNA•Cell free expression of Felixounavirus bacteriophages•Escherichia coli based cell free expression of Salmonella specific phage |
| doi_str_mv | 10.1016/j.mimet.2024.106920 |
| format | Article |
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| ispartof | Journal of microbiological methods, 2024-05, Vol.220, p.106920, Article 106920 |
| issn | 0167-7012 1872-8359 1872-8359 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Bacteriophages - genetics Cell-free expression Escherichia coli - genetics felixO1 Genome, Viral Host Specificity Phage Salmonella Salmonella - genetics Salmonella Phages - genetics |
| title | Cell-free synthesis of the Salmonella specific broad host range bacteriophage, felixO1 |
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