Cell-free synthesis of the Salmonella specific broad host range bacteriophage, felixO1

Phage-based biocontrol of foodborne Salmonella is limited by the requisite use of Salmonella to propagate the phages. This limitation can be circumvented by producing Salmonella phages using a cell-free gene expression system (CFE) with a non-pathogenic chassis. Here, we produce the Salmonella phage felixO1 using an E. coli-based CFE system. •Simple method for obtaining high-concentration phage genomic DNA•Cell free expression of Felixounavirus bacteriophages•Escherichia coli based cell free expression of Salmonella specific phage