Accumulation of docosapentaenoic acid (n-3 DPA) in a novel isolate of the marine ichthyosporean Sphaeroforma arctica
Objective Currently, there is lack of a consistent and highly enriched source for docosapentaenoic acid (n-3 DPA, C22:5), and this work report the isolation of microorganism that naturally produces n-3 DPA. Results In this work, we screened microorganisms in our culture collections with the goal to...
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| Veröffentlicht in: | Biotechnology letters 2024-06, Vol.46 (3), p.373-383 |
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| container_title | Biotechnology letters |
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| creator | Yan, Qiang Wilson Liu, Ying-Chun Barrett, Christa Haake, Kelly Seeler, Daniel May, Oliver Zirkle, Ross |
| description | Objective
Currently, there is lack of a consistent and highly enriched source for docosapentaenoic acid (n-3 DPA, C22:5), and this work report the isolation of microorganism that naturally produces n-3 DPA.
Results
In this work, we screened microorganisms in our culture collections with the goal to isolate a strain with high levels of n-3 DPA. We isolated a strain of
Sphaeroforma arctica
that produces up to 11% n-3 DPA in total fatty acid and has a high n-3 DPA to DHA/EPA ratio. The cell growth of the isolated strain was characterized using microscopy imaging and flow cytometer technologies to confirm the coenocytic pattern of cell divisions previously described in
S. arctica
. Our novel isolate of
S. arctica
grew more robustly and produced significantly more n-3 DPA compared to previously isolated and described strains indicating the uniqueness of the discovered strain.
Conclusion
Overall, this work reports a first isolate n-3 DPA producing microorganism and establishes the foundation for future strain improvement and elucidation of the physiological function of this LC-PUFA for human nutrition and health. |
| doi_str_mv | 10.1007/s10529-024-03472-5 |
| format | Article |
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Currently, there is lack of a consistent and highly enriched source for docosapentaenoic acid (n-3 DPA, C22:5), and this work report the isolation of microorganism that naturally produces n-3 DPA.
Results
In this work, we screened microorganisms in our culture collections with the goal to isolate a strain with high levels of n-3 DPA. We isolated a strain of
Sphaeroforma arctica
that produces up to 11% n-3 DPA in total fatty acid and has a high n-3 DPA to DHA/EPA ratio. The cell growth of the isolated strain was characterized using microscopy imaging and flow cytometer technologies to confirm the coenocytic pattern of cell divisions previously described in
S. arctica
. Our novel isolate of
S. arctica
grew more robustly and produced significantly more n-3 DPA compared to previously isolated and described strains indicating the uniqueness of the discovered strain.
Conclusion
Overall, this work reports a first isolate n-3 DPA producing microorganism and establishes the foundation for future strain improvement and elucidation of the physiological function of this LC-PUFA for human nutrition and health.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-024-03472-5</identifier><identifier>PMID: 38493279</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Applied Microbiology ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Cell culture ; Cell division ; Cell growth ; Culture collections ; docosapentaenoic acid ; Fatty acids ; flow cytometry ; Human nutrition ; Hypotheses ; Life Sciences ; Microbiology ; Microorganisms ; microscopy ; Nutrition ; Oils & fats ; Original Research Paper ; Polyunsaturated fatty acids</subject><ispartof>Biotechnology letters, 2024-06, Vol.46 (3), p.373-383</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c359t-ec4e5c76000436d354a113748787e99da1befd3d8bc958f8f5cf08bc1ede8c0c3</cites><orcidid>0009-0007-5245-8274</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10529-024-03472-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10529-024-03472-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38493279$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yan, Qiang Wilson</creatorcontrib><creatorcontrib>Liu, Ying-Chun</creatorcontrib><creatorcontrib>Barrett, Christa</creatorcontrib><creatorcontrib>Haake, Kelly</creatorcontrib><creatorcontrib>Seeler, Daniel</creatorcontrib><creatorcontrib>May, Oliver</creatorcontrib><creatorcontrib>Zirkle, Ross</creatorcontrib><title>Accumulation of docosapentaenoic acid (n-3 DPA) in a novel isolate of the marine ichthyosporean Sphaeroforma arctica</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><addtitle>Biotechnol Lett</addtitle><description>Objective
Currently, there is lack of a consistent and highly enriched source for docosapentaenoic acid (n-3 DPA, C22:5), and this work report the isolation of microorganism that naturally produces n-3 DPA.
Results
In this work, we screened microorganisms in our culture collections with the goal to isolate a strain with high levels of n-3 DPA. We isolated a strain of
Sphaeroforma arctica
that produces up to 11% n-3 DPA in total fatty acid and has a high n-3 DPA to DHA/EPA ratio. The cell growth of the isolated strain was characterized using microscopy imaging and flow cytometer technologies to confirm the coenocytic pattern of cell divisions previously described in
S. arctica
. Our novel isolate of
S. arctica
grew more robustly and produced significantly more n-3 DPA compared to previously isolated and described strains indicating the uniqueness of the discovered strain.
Conclusion
Overall, this work reports a first isolate n-3 DPA producing microorganism and establishes the foundation for future strain improvement and elucidation of the physiological function of this LC-PUFA for human nutrition and health.</description><subject>Applied Microbiology</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell culture</subject><subject>Cell division</subject><subject>Cell growth</subject><subject>Culture collections</subject><subject>docosapentaenoic acid</subject><subject>Fatty acids</subject><subject>flow cytometry</subject><subject>Human nutrition</subject><subject>Hypotheses</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>microscopy</subject><subject>Nutrition</subject><subject>Oils & fats</subject><subject>Original Research Paper</subject><subject>Polyunsaturated fatty acids</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkcFuFSEUhomxsbfVF3BhSNzUBfUwwDAsb6pWkyaaqGvCZc54aWZghJkmfftSb9XEhV0Rwvf_B_gIecnhnAPot4WDagyDRjIQUjdMPSEbrrRgrdbtU7IBLjlT0jTH5KSUawAwGvQzciw6aUSjzYYsW-_XaR3dElKkaaB98qm4GePiMKbgqfOhp2eRCfruy_YNDZE6GtMNjjSUVHN4n1r2SCeXQ0Qa_H7Z36Yyp4wu0q_z3mFOQ8qToy77JXj3nBwNbiz44mE9Jd8_vP928ZFdfb78dLG9Yl4oszD0EpXXbb23FG0vlHScCy073Wk0pnd8h0Mv-m7njeqGblB-gLrh2GPnwYtTcnbonXP6uWJZ7BSKx3F0EdNarOBKtLyTwB9FmzqhMYK3pqKv_0Gv05pjfYgVILUEBR1UqjlQPqdSMg52zqF-0a3lYO_12YM-W_XZX_qsqqFXD9XrbsL-T-S3rwqIA1DqUfyB-e_s_9TeAZPmpRM</recordid><startdate>20240601</startdate><enddate>20240601</enddate><creator>Yan, Qiang Wilson</creator><creator>Liu, Ying-Chun</creator><creator>Barrett, Christa</creator><creator>Haake, Kelly</creator><creator>Seeler, Daniel</creator><creator>May, Oliver</creator><creator>Zirkle, Ross</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>L7M</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0009-0007-5245-8274</orcidid></search><sort><creationdate>20240601</creationdate><title>Accumulation of docosapentaenoic acid (n-3 DPA) in a novel isolate of the marine ichthyosporean Sphaeroforma arctica</title><author>Yan, Qiang Wilson ; Liu, Ying-Chun ; Barrett, Christa ; Haake, Kelly ; Seeler, Daniel ; May, Oliver ; Zirkle, Ross</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-ec4e5c76000436d354a113748787e99da1befd3d8bc958f8f5cf08bc1ede8c0c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Applied Microbiology</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell culture</topic><topic>Cell division</topic><topic>Cell growth</topic><topic>Culture collections</topic><topic>docosapentaenoic acid</topic><topic>Fatty acids</topic><topic>flow cytometry</topic><topic>Human nutrition</topic><topic>Hypotheses</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>microscopy</topic><topic>Nutrition</topic><topic>Oils & fats</topic><topic>Original Research Paper</topic><topic>Polyunsaturated fatty acids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yan, Qiang Wilson</creatorcontrib><creatorcontrib>Liu, Ying-Chun</creatorcontrib><creatorcontrib>Barrett, Christa</creatorcontrib><creatorcontrib>Haake, Kelly</creatorcontrib><creatorcontrib>Seeler, Daniel</creatorcontrib><creatorcontrib>May, Oliver</creatorcontrib><creatorcontrib>Zirkle, Ross</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yan, Qiang Wilson</au><au>Liu, Ying-Chun</au><au>Barrett, Christa</au><au>Haake, Kelly</au><au>Seeler, Daniel</au><au>May, Oliver</au><au>Zirkle, Ross</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Accumulation of docosapentaenoic acid (n-3 DPA) in a novel isolate of the marine ichthyosporean Sphaeroforma arctica</atitle><jtitle>Biotechnology letters</jtitle><stitle>Biotechnol Lett</stitle><addtitle>Biotechnol Lett</addtitle><date>2024-06-01</date><risdate>2024</risdate><volume>46</volume><issue>3</issue><spage>373</spage><epage>383</epage><pages>373-383</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><abstract>Objective
Currently, there is lack of a consistent and highly enriched source for docosapentaenoic acid (n-3 DPA, C22:5), and this work report the isolation of microorganism that naturally produces n-3 DPA.
Results
In this work, we screened microorganisms in our culture collections with the goal to isolate a strain with high levels of n-3 DPA. We isolated a strain of
Sphaeroforma arctica
that produces up to 11% n-3 DPA in total fatty acid and has a high n-3 DPA to DHA/EPA ratio. The cell growth of the isolated strain was characterized using microscopy imaging and flow cytometer technologies to confirm the coenocytic pattern of cell divisions previously described in
S. arctica
. Our novel isolate of
S. arctica
grew more robustly and produced significantly more n-3 DPA compared to previously isolated and described strains indicating the uniqueness of the discovered strain.
Conclusion
Overall, this work reports a first isolate n-3 DPA producing microorganism and establishes the foundation for future strain improvement and elucidation of the physiological function of this LC-PUFA for human nutrition and health.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>38493279</pmid><doi>10.1007/s10529-024-03472-5</doi><tpages>11</tpages><orcidid>https://orcid.org/0009-0007-5245-8274</orcidid></addata></record> |
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| ispartof | Biotechnology letters, 2024-06, Vol.46 (3), p.373-383 |
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| subjects | Applied Microbiology Biochemistry Biomedical and Life Sciences Biotechnology Cell culture Cell division Cell growth Culture collections docosapentaenoic acid Fatty acids flow cytometry Human nutrition Hypotheses Life Sciences Microbiology Microorganisms microscopy Nutrition Oils & fats Original Research Paper Polyunsaturated fatty acids |
| title | Accumulation of docosapentaenoic acid (n-3 DPA) in a novel isolate of the marine ichthyosporean Sphaeroforma arctica |
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