Investigating the Effects and Mechanisms of Cyclomorusin on Osteoclasts in a High Glucose Environment
Diabetes mellitus is an endocrine disease characterized by prolonged hyperglycemia. Prolonged high blood sugar levels interfere with the differentiation and maturation process of OBs and OCs, leading to the onset of osteoporosis. However, OCs differentiation and maturation is a complex regulatory pr...
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| Veröffentlicht in: | Chemistry & biodiversity 2024-05, Vol.21 (5), p.e202301741-n/a |
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| creator | Guo, Dong‐Gui Zhu, Jun Wang, Hui‐Juan Pan, Bo‐Wen |
| description | Diabetes mellitus is an endocrine disease characterized by prolonged hyperglycemia. Prolonged high blood sugar levels interfere with the differentiation and maturation process of OBs and OCs, leading to the onset of osteoporosis. However, OCs differentiation and maturation is a complex regulatory process. In this study, we used a co‐culture system of RAW264.7 and MC3T3‐E1 cells under HG concentration to explore the effect of CYM on OCs in a HG environment. The effects of CYM on the formation and function of OCs were observed using TRAP‐positive cell counts and bone resorption pits. Then, mRNA and protein expression levels of OCs‐related genes were detected by real‐time qPCR and western blotting. The results showed that CYM had an inhibitory effect on OCs differentiation and bone resorption, reduced mRNAs expression of OCs‐associated genes, and downregulated RANKL/RANK/TRAF6 pathway that mediates OCs differentiation. CYM could be a promising natural compound against diabetic osteoporosis. |
| doi_str_mv | 10.1002/cbdv.202301741 |
| format | Article |
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Prolonged high blood sugar levels interfere with the differentiation and maturation process of OBs and OCs, leading to the onset of osteoporosis. However, OCs differentiation and maturation is a complex regulatory process. In this study, we used a co‐culture system of RAW264.7 and MC3T3‐E1 cells under HG concentration to explore the effect of CYM on OCs in a HG environment. The effects of CYM on the formation and function of OCs were observed using TRAP‐positive cell counts and bone resorption pits. Then, mRNA and protein expression levels of OCs‐related genes were detected by real‐time qPCR and western blotting. The results showed that CYM had an inhibitory effect on OCs differentiation and bone resorption, reduced mRNAs expression of OCs‐associated genes, and downregulated RANKL/RANK/TRAF6 pathway that mediates OCs differentiation. CYM could be a promising natural compound against diabetic osteoporosis.</description><identifier>ISSN: 1612-1872</identifier><identifier>EISSN: 1612-1880</identifier><identifier>DOI: 10.1002/cbdv.202301741</identifier><identifier>PMID: 38477870</identifier><language>eng</language><publisher>Switzerland: Wiley Subscription Services, Inc</publisher><subject>Animals ; Blood levels ; Bone resorption ; Bone Resorption - drug therapy ; Bone Resorption - metabolism ; Cell culture ; Cell Differentiation - drug effects ; Cells, Cultured ; Cyclomorusin ; Diabetes mellitus ; Differentiation ; Dose-Response Relationship, Drug ; Endocrine disorders ; Gene expression ; Genes ; Glucose - metabolism ; Glucose - pharmacology ; High glucose concentrations ; Hyperglycemia ; Maturation ; Mice ; mRNA ; Osteoclasts ; Osteoclasts - cytology ; Osteoclasts - drug effects ; Osteoclasts - metabolism ; Osteoporosis ; RANK Ligand - metabolism ; RANKL/RANK/TRAF6 pathway ; RAW 264.7 Cells ; Receptor Activator of Nuclear Factor-kappa B - genetics ; Receptor Activator of Nuclear Factor-kappa B - metabolism ; TNF Receptor-Associated Factor 6 - metabolism ; TRAF6 protein ; Western blotting</subject><ispartof>Chemistry & biodiversity, 2024-05, Vol.21 (5), p.e202301741-n/a</ispartof><rights>2024 Wiley-VHCA AG, Zurich, Switzerland</rights><rights>2024 Wiley-VHCA AG, Zurich, Switzerland.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3281-25d9cb38be2ecc1b756630dc6e5c8d4e6822cd9e6f0c3140fe2339a7c77e35c43</cites><orcidid>0009-0007-1140-1802</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbdv.202301741$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbdv.202301741$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38477870$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Dong‐Gui</creatorcontrib><creatorcontrib>Zhu, Jun</creatorcontrib><creatorcontrib>Wang, Hui‐Juan</creatorcontrib><creatorcontrib>Pan, Bo‐Wen</creatorcontrib><title>Investigating the Effects and Mechanisms of Cyclomorusin on Osteoclasts in a High Glucose Environment</title><title>Chemistry & biodiversity</title><addtitle>Chem Biodivers</addtitle><description>Diabetes mellitus is an endocrine disease characterized by prolonged hyperglycemia. Prolonged high blood sugar levels interfere with the differentiation and maturation process of OBs and OCs, leading to the onset of osteoporosis. However, OCs differentiation and maturation is a complex regulatory process. In this study, we used a co‐culture system of RAW264.7 and MC3T3‐E1 cells under HG concentration to explore the effect of CYM on OCs in a HG environment. The effects of CYM on the formation and function of OCs were observed using TRAP‐positive cell counts and bone resorption pits. Then, mRNA and protein expression levels of OCs‐related genes were detected by real‐time qPCR and western blotting. The results showed that CYM had an inhibitory effect on OCs differentiation and bone resorption, reduced mRNAs expression of OCs‐associated genes, and downregulated RANKL/RANK/TRAF6 pathway that mediates OCs differentiation. CYM could be a promising natural compound against diabetic osteoporosis.</description><subject>Animals</subject><subject>Blood levels</subject><subject>Bone resorption</subject><subject>Bone Resorption - drug therapy</subject><subject>Bone Resorption - metabolism</subject><subject>Cell culture</subject><subject>Cell Differentiation - drug effects</subject><subject>Cells, Cultured</subject><subject>Cyclomorusin</subject><subject>Diabetes mellitus</subject><subject>Differentiation</subject><subject>Dose-Response Relationship, Drug</subject><subject>Endocrine disorders</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Glucose - metabolism</subject><subject>Glucose - pharmacology</subject><subject>High glucose concentrations</subject><subject>Hyperglycemia</subject><subject>Maturation</subject><subject>Mice</subject><subject>mRNA</subject><subject>Osteoclasts</subject><subject>Osteoclasts - cytology</subject><subject>Osteoclasts - drug effects</subject><subject>Osteoclasts - metabolism</subject><subject>Osteoporosis</subject><subject>RANK Ligand - metabolism</subject><subject>RANKL/RANK/TRAF6 pathway</subject><subject>RAW 264.7 Cells</subject><subject>Receptor Activator of Nuclear Factor-kappa B - genetics</subject><subject>Receptor Activator of Nuclear Factor-kappa B - metabolism</subject><subject>TNF Receptor-Associated Factor 6 - metabolism</subject><subject>TRAF6 protein</subject><subject>Western blotting</subject><issn>1612-1872</issn><issn>1612-1880</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1P2zAYh61paJSPK0dkaZddWvyRxM4Rug6QirgAV8t586Y1SmwWJ0X97-eq0Em77GTrp8eP_L4_Qi44m3HGxBVU9WYmmJCMq4x_IRNecDHlWrOvh7sSx-QkxtfEp1x_I8dSZ0ppxSYE7_0G4-BWdnB-RYc10kXTIAyRWl_TB4S19S52kYaGzrfQhi70Y3SeBk8f44ABWhsTnRJL79xqTW_bEUJMHr9xffAd-uGMHDW2jXj-cZ6S51-Lp_nddPl4ez-_Xk5BCs2nIq9LqKSuUCAAr1ReFJLVUGAOus6w0EJAXWLRMJA8Yw0KKUurQCmUOWTylPzYe9_68HtMc5nORcC2tR7DGI0ok1GLPNMJ_f4P-hrG3qffGcnyrMwzJvNEzfYU9CHGHhvz1rvO9lvDmdkVYHYFmEMB6cHlh3asOqwP-OfGE1DugXfX4vY_OjO_-fnyV_4H0LOSRA</recordid><startdate>202405</startdate><enddate>202405</enddate><creator>Guo, Dong‐Gui</creator><creator>Zhu, Jun</creator><creator>Wang, Hui‐Juan</creator><creator>Pan, Bo‐Wen</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0009-0007-1140-1802</orcidid></search><sort><creationdate>202405</creationdate><title>Investigating the Effects and Mechanisms of Cyclomorusin on Osteoclasts in a High Glucose Environment</title><author>Guo, Dong‐Gui ; Zhu, Jun ; Wang, Hui‐Juan ; Pan, Bo‐Wen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3281-25d9cb38be2ecc1b756630dc6e5c8d4e6822cd9e6f0c3140fe2339a7c77e35c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Blood levels</topic><topic>Bone resorption</topic><topic>Bone Resorption - drug therapy</topic><topic>Bone Resorption - metabolism</topic><topic>Cell culture</topic><topic>Cell Differentiation - drug effects</topic><topic>Cells, Cultured</topic><topic>Cyclomorusin</topic><topic>Diabetes mellitus</topic><topic>Differentiation</topic><topic>Dose-Response Relationship, Drug</topic><topic>Endocrine disorders</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Glucose - metabolism</topic><topic>Glucose - pharmacology</topic><topic>High glucose concentrations</topic><topic>Hyperglycemia</topic><topic>Maturation</topic><topic>Mice</topic><topic>mRNA</topic><topic>Osteoclasts</topic><topic>Osteoclasts - cytology</topic><topic>Osteoclasts - drug effects</topic><topic>Osteoclasts - metabolism</topic><topic>Osteoporosis</topic><topic>RANK Ligand - metabolism</topic><topic>RANKL/RANK/TRAF6 pathway</topic><topic>RAW 264.7 Cells</topic><topic>Receptor Activator of Nuclear Factor-kappa B - genetics</topic><topic>Receptor Activator of Nuclear Factor-kappa B - metabolism</topic><topic>TNF Receptor-Associated Factor 6 - metabolism</topic><topic>TRAF6 protein</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Dong‐Gui</creatorcontrib><creatorcontrib>Zhu, Jun</creatorcontrib><creatorcontrib>Wang, Hui‐Juan</creatorcontrib><creatorcontrib>Pan, Bo‐Wen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chemistry & biodiversity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Dong‐Gui</au><au>Zhu, Jun</au><au>Wang, Hui‐Juan</au><au>Pan, Bo‐Wen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigating the Effects and Mechanisms of Cyclomorusin on Osteoclasts in a High Glucose Environment</atitle><jtitle>Chemistry & biodiversity</jtitle><addtitle>Chem Biodivers</addtitle><date>2024-05</date><risdate>2024</risdate><volume>21</volume><issue>5</issue><spage>e202301741</spage><epage>n/a</epage><pages>e202301741-n/a</pages><issn>1612-1872</issn><eissn>1612-1880</eissn><abstract>Diabetes mellitus is an endocrine disease characterized by prolonged hyperglycemia. Prolonged high blood sugar levels interfere with the differentiation and maturation process of OBs and OCs, leading to the onset of osteoporosis. However, OCs differentiation and maturation is a complex regulatory process. In this study, we used a co‐culture system of RAW264.7 and MC3T3‐E1 cells under HG concentration to explore the effect of CYM on OCs in a HG environment. The effects of CYM on the formation and function of OCs were observed using TRAP‐positive cell counts and bone resorption pits. Then, mRNA and protein expression levels of OCs‐related genes were detected by real‐time qPCR and western blotting. The results showed that CYM had an inhibitory effect on OCs differentiation and bone resorption, reduced mRNAs expression of OCs‐associated genes, and downregulated RANKL/RANK/TRAF6 pathway that mediates OCs differentiation. CYM could be a promising natural compound against diabetic osteoporosis.</abstract><cop>Switzerland</cop><pub>Wiley Subscription Services, Inc</pub><pmid>38477870</pmid><doi>10.1002/cbdv.202301741</doi><tpages>7</tpages><orcidid>https://orcid.org/0009-0007-1140-1802</orcidid></addata></record> |
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| subjects | Animals Blood levels Bone resorption Bone Resorption - drug therapy Bone Resorption - metabolism Cell culture Cell Differentiation - drug effects Cells, Cultured Cyclomorusin Diabetes mellitus Differentiation Dose-Response Relationship, Drug Endocrine disorders Gene expression Genes Glucose - metabolism Glucose - pharmacology High glucose concentrations Hyperglycemia Maturation Mice mRNA Osteoclasts Osteoclasts - cytology Osteoclasts - drug effects Osteoclasts - metabolism Osteoporosis RANK Ligand - metabolism RANKL/RANK/TRAF6 pathway RAW 264.7 Cells Receptor Activator of Nuclear Factor-kappa B - genetics Receptor Activator of Nuclear Factor-kappa B - metabolism TNF Receptor-Associated Factor 6 - metabolism TRAF6 protein Western blotting |
| title | Investigating the Effects and Mechanisms of Cyclomorusin on Osteoclasts in a High Glucose Environment |
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