l-Glutamate biosensor for estimation of the taste of tomato specimens
An amperometric biosensor has been developed for measurement of Umami, or the taste based on the amount of l-glutamate, in tomato foods. The biosensor is based on an enzyme-mediator system in which l-glutamate oxidase is used for biochemical oxidation of l-glutamate and a tetrafulvalene-tetracyanoqu...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2006-09, Vol.386 (2), p.220-227 |
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| creator | Pauliukaite, Rasa Zhylyak, Gleb Citterio, Daniel Spichiger-Keller, Ursula E |
| description | An amperometric biosensor has been developed for measurement of Umami, or the taste based on the amount of l-glutamate, in tomato foods. The biosensor is based on an enzyme-mediator system in which l-glutamate oxidase is used for biochemical oxidation of l-glutamate and a tetrafulvalene-tetracyanoquinodimethane (TTF-TCNQ) paste, prepared from the mixture of TTF-TCNQ salt, graphite powder, and silicone oil, serves as the mediator. The limit of detection, calculated by use of a four-parameter logistic model, was 0.05 mmol L-¹, and the limit of quantification was 0.15 mmol L-¹. The correlation coefficient (R ²) was 0.990 and the relative standard deviation was no more than 1% (n=5). The response time (τ ₉₅) was 20-50 s, depending on concentration. The repeatability of the sensor was better than 5% (n=10). The sensor developed was stable for more than ten days. [graphic removed] |
| doi_str_mv | 10.1007/s00216-006-0656-2 |
| format | Article |
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The biosensor is based on an enzyme-mediator system in which l-glutamate oxidase is used for biochemical oxidation of l-glutamate and a tetrafulvalene-tetracyanoquinodimethane (TTF-TCNQ) paste, prepared from the mixture of TTF-TCNQ salt, graphite powder, and silicone oil, serves as the mediator. The limit of detection, calculated by use of a four-parameter logistic model, was 0.05 mmol L-¹, and the limit of quantification was 0.15 mmol L-¹. The correlation coefficient (R ²) was 0.990 and the relative standard deviation was no more than 1% (n=5). The response time (τ ₉₅) was 20-50 s, depending on concentration. The repeatability of the sensor was better than 5% (n=10). The sensor developed was stable for more than ten days. 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The biosensor is based on an enzyme-mediator system in which l-glutamate oxidase is used for biochemical oxidation of l-glutamate and a tetrafulvalene-tetracyanoquinodimethane (TTF-TCNQ) paste, prepared from the mixture of TTF-TCNQ salt, graphite powder, and silicone oil, serves as the mediator. The limit of detection, calculated by use of a four-parameter logistic model, was 0.05 mmol L-¹, and the limit of quantification was 0.15 mmol L-¹. The correlation coefficient (R ²) was 0.990 and the relative standard deviation was no more than 1% (n=5). The response time (τ ₉₅) was 20-50 s, depending on concentration. The repeatability of the sensor was better than 5% (n=10). The sensor developed was stable for more than ten days. 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The biosensor is based on an enzyme-mediator system in which l-glutamate oxidase is used for biochemical oxidation of l-glutamate and a tetrafulvalene-tetracyanoquinodimethane (TTF-TCNQ) paste, prepared from the mixture of TTF-TCNQ salt, graphite powder, and silicone oil, serves as the mediator. The limit of detection, calculated by use of a four-parameter logistic model, was 0.05 mmol L-¹, and the limit of quantification was 0.15 mmol L-¹. The correlation coefficient (R ²) was 0.990 and the relative standard deviation was no more than 1% (n=5). The response time (τ ₉₅) was 20-50 s, depending on concentration. The repeatability of the sensor was better than 5% (n=10). The sensor developed was stable for more than ten days. [graphic removed]</abstract><cop>Germany</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>16917673</pmid><doi>10.1007/s00216-006-0656-2</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Amino Acid Oxidoreductases - analysis Amino Acid Oxidoreductases - metabolism Amperometric biosensors Biosensing Techniques - methods Electrochemistry Electrodes Glutamates - chemistry Graphite - chemistry Heterocyclic Compounds - chemistry l-Glutamate determination l-Glutamate oxidase Lycopersicon esculentum Lycopersicon esculentum - chemistry Nitriles - chemistry Oxidation-Reduction Silicone Oils - chemistry Taste - physiology Time Factors TTF-TCNQ umami |
| title | l-Glutamate biosensor for estimation of the taste of tomato specimens |
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