Effect of curing regime on the cytotoxicity of resin-modified glass-ionomer lining cements applied to an odontoblast-cell line
The aim of this in vitro study was to evaluate the cytotoxicity of resin-modified glass-ionomer lining cements submitted to different curing regimes and applied to an immortalized odontoblast-cell line (MDPC-23). Forty round-shaped specimens of each experimental material (Fuji Lining LC and Vitrebon...
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| Veröffentlicht in: | Dental materials 2006-09, Vol.22 (9), p.864-869 |
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| creator | Aranha, Andreza M.F. Giro, Elisa M.A. Souza, Pedro P.C. Hebling, Josimeri de Souza Costa, Carlos A. |
| description | The aim of this in vitro study was to evaluate the cytotoxicity of resin-modified glass-ionomer lining cements submitted to different curing regimes and applied to an immortalized odontoblast-cell line (MDPC-23).
Forty round-shaped specimens of each experimental material (Fuji Lining LC and Vitrebond) were prepared. They were light-cured for the manufacturers’ recommended time (MRT
=
30
s), under-cured (0.5 MRT
=
15
s), over-cured (1.5 MRT
=
45
s) or allowed to dark cure (0 MRT). Sterilized filter papers soaked with either 5
μL of PBS or HEMA were used as negative and positive control, respectively. After placing the specimens individually in wells of 24-well dishes, odontoblast-like cells MDPC-23 (30,000
cells/cm
2) were plated in each well and incubated for 72
h in a humidified incubator at 37
°C with 5% CO
2 and 95% air. The cytotoxicity was evaluated by the cell metabolism (MTT assay) and cell morphology (SEM).
Fuji Lining LC was less cytotoxic than Vitrebond (
p
<
0.05) in all the experimental conditions. However, the cytotoxicity of Fuji Lining LC was noticeably increased in the absence of light-curing while the same was not observed for Vitrebond. The length of light-curing (15, 30 or 45
s) did not influence the toxicity of both lining materials when they were applied on the odontoblast-cell line MDPC-23.
The light-activation plays an important role in reducing the cytotoxicity of Fuji Lining LC. Following the manufacturer’ recommendation regarding the light-curing regime may prevent toxic effect to the pulp cells. |
| doi_str_mv | 10.1016/j.dental.2005.11.015 |
| format | Article |
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Forty round-shaped specimens of each experimental material (Fuji Lining LC and Vitrebond) were prepared. They were light-cured for the manufacturers’ recommended time (MRT
=
30
s), under-cured (0.5 MRT
=
15
s), over-cured (1.5 MRT
=
45
s) or allowed to dark cure (0 MRT). Sterilized filter papers soaked with either 5
μL of PBS or HEMA were used as negative and positive control, respectively. After placing the specimens individually in wells of 24-well dishes, odontoblast-like cells MDPC-23 (30,000
cells/cm
2) were plated in each well and incubated for 72
h in a humidified incubator at 37
°C with 5% CO
2 and 95% air. The cytotoxicity was evaluated by the cell metabolism (MTT assay) and cell morphology (SEM).
Fuji Lining LC was less cytotoxic than Vitrebond (
p
<
0.05) in all the experimental conditions. However, the cytotoxicity of Fuji Lining LC was noticeably increased in the absence of light-curing while the same was not observed for Vitrebond. The length of light-curing (15, 30 or 45
s) did not influence the toxicity of both lining materials when they were applied on the odontoblast-cell line MDPC-23.
The light-activation plays an important role in reducing the cytotoxicity of Fuji Lining LC. Following the manufacturer’ recommendation regarding the light-curing regime may prevent toxic effect to the pulp cells.</description><identifier>ISSN: 0109-5641</identifier><identifier>EISSN: 1879-0097</identifier><identifier>DOI: 10.1016/j.dental.2005.11.015</identifier><identifier>PMID: 16388848</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Cell Line, Transformed ; Cell Shape - drug effects ; Coloring Agents - metabolism ; Curing regime ; Cytotoxicity ; Dental Cavity Lining ; Dentin-Bonding Agents - radiation effects ; Dentin-Bonding Agents - toxicity ; Glass Ionomer Cements - chemistry ; Glass Ionomer Cements - radiation effects ; Glass Ionomer Cements - toxicity ; Glass-ionomer cements ; HEMA ; Light ; Methacrylates - toxicity ; Mice ; Microscopy, Electron, Scanning ; Odontoblast ; Odontoblasts - drug effects ; Phase Transition ; Resins, Synthetic - radiation effects ; Resins, Synthetic - toxicity ; Tetrazolium Salts - metabolism</subject><ispartof>Dental materials, 2006-09, Vol.22 (9), p.864-869</ispartof><rights>2005 Academy of Dental Materials</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-957db399df7ed2c1b86b801d721cba0b5d5783edad47b69395b9a992f86bf4c43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.dental.2005.11.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16388848$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aranha, Andreza M.F.</creatorcontrib><creatorcontrib>Giro, Elisa M.A.</creatorcontrib><creatorcontrib>Souza, Pedro P.C.</creatorcontrib><creatorcontrib>Hebling, Josimeri</creatorcontrib><creatorcontrib>de Souza Costa, Carlos A.</creatorcontrib><title>Effect of curing regime on the cytotoxicity of resin-modified glass-ionomer lining cements applied to an odontoblast-cell line</title><title>Dental materials</title><addtitle>Dent Mater</addtitle><description>The aim of this in vitro study was to evaluate the cytotoxicity of resin-modified glass-ionomer lining cements submitted to different curing regimes and applied to an immortalized odontoblast-cell line (MDPC-23).
Forty round-shaped specimens of each experimental material (Fuji Lining LC and Vitrebond) were prepared. They were light-cured for the manufacturers’ recommended time (MRT
=
30
s), under-cured (0.5 MRT
=
15
s), over-cured (1.5 MRT
=
45
s) or allowed to dark cure (0 MRT). Sterilized filter papers soaked with either 5
μL of PBS or HEMA were used as negative and positive control, respectively. After placing the specimens individually in wells of 24-well dishes, odontoblast-like cells MDPC-23 (30,000
cells/cm
2) were plated in each well and incubated for 72
h in a humidified incubator at 37
°C with 5% CO
2 and 95% air. The cytotoxicity was evaluated by the cell metabolism (MTT assay) and cell morphology (SEM).
Fuji Lining LC was less cytotoxic than Vitrebond (
p
<
0.05) in all the experimental conditions. However, the cytotoxicity of Fuji Lining LC was noticeably increased in the absence of light-curing while the same was not observed for Vitrebond. The length of light-curing (15, 30 or 45
s) did not influence the toxicity of both lining materials when they were applied on the odontoblast-cell line MDPC-23.
The light-activation plays an important role in reducing the cytotoxicity of Fuji Lining LC. Following the manufacturer’ recommendation regarding the light-curing regime may prevent toxic effect to the pulp cells.</description><subject>Animals</subject><subject>Cell Line, Transformed</subject><subject>Cell Shape - drug effects</subject><subject>Coloring Agents - metabolism</subject><subject>Curing regime</subject><subject>Cytotoxicity</subject><subject>Dental Cavity Lining</subject><subject>Dentin-Bonding Agents - radiation effects</subject><subject>Dentin-Bonding Agents - toxicity</subject><subject>Glass Ionomer Cements - chemistry</subject><subject>Glass Ionomer Cements - radiation effects</subject><subject>Glass Ionomer Cements - toxicity</subject><subject>Glass-ionomer cements</subject><subject>HEMA</subject><subject>Light</subject><subject>Methacrylates - toxicity</subject><subject>Mice</subject><subject>Microscopy, Electron, Scanning</subject><subject>Odontoblast</subject><subject>Odontoblasts - drug effects</subject><subject>Phase Transition</subject><subject>Resins, Synthetic - radiation effects</subject><subject>Resins, Synthetic - toxicity</subject><subject>Tetrazolium Salts - metabolism</subject><issn>0109-5641</issn><issn>1879-0097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD2P1DAQhi0E4vYO_gFCrugSPEmcxA0SOh1w0kk0UFv-GC9eJfZiexHb8NtxtCvRUU3zvO_MPIS8AdYCg_H9obUYilrajjHeArQM-DOyg3kSDWNiek52DJho-DjADbnN-cAYGzoBL8kNjP08z8O8I38enENTaHTUnJIPe5pw71ekMdDyA6k5l1jib298OW9QwuxDs0brnUdL94vKufExxBUTXXzYGgyu9bJM1fG4bFCJVAUabQwl6hoojcFl2Wh8RV44tWR8fZ135Punh2_3X5qnr58f7z8-NWbgU2kEn6zuhbBuQtsZ0POoZwZ26sBoxTS3fJp7tMoOkx5FL7gWSojOVc4NZujvyLtL7zHFnyfMRa4-b1eogPGUZSc4cBBjBYcLaFLMOaGTx-RXlc4SmNy8y4O8eJebdwkgq_cae3vtP-kV7b_QVXQFPlwArF_-8phkNh6DQetT9S9t9P_f8Bfjz5i5</recordid><startdate>20060901</startdate><enddate>20060901</enddate><creator>Aranha, Andreza M.F.</creator><creator>Giro, Elisa M.A.</creator><creator>Souza, Pedro P.C.</creator><creator>Hebling, Josimeri</creator><creator>de Souza Costa, Carlos A.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20060901</creationdate><title>Effect of curing regime on the cytotoxicity of resin-modified glass-ionomer lining cements applied to an odontoblast-cell line</title><author>Aranha, Andreza M.F. ; Giro, Elisa M.A. ; Souza, Pedro P.C. ; Hebling, Josimeri ; de Souza Costa, Carlos A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-957db399df7ed2c1b86b801d721cba0b5d5783edad47b69395b9a992f86bf4c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Cell Line, Transformed</topic><topic>Cell Shape - drug effects</topic><topic>Coloring Agents - metabolism</topic><topic>Curing regime</topic><topic>Cytotoxicity</topic><topic>Dental Cavity Lining</topic><topic>Dentin-Bonding Agents - radiation effects</topic><topic>Dentin-Bonding Agents - toxicity</topic><topic>Glass Ionomer Cements - chemistry</topic><topic>Glass Ionomer Cements - radiation effects</topic><topic>Glass Ionomer Cements - toxicity</topic><topic>Glass-ionomer cements</topic><topic>HEMA</topic><topic>Light</topic><topic>Methacrylates - toxicity</topic><topic>Mice</topic><topic>Microscopy, Electron, Scanning</topic><topic>Odontoblast</topic><topic>Odontoblasts - drug effects</topic><topic>Phase Transition</topic><topic>Resins, Synthetic - radiation effects</topic><topic>Resins, Synthetic - toxicity</topic><topic>Tetrazolium Salts - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aranha, Andreza M.F.</creatorcontrib><creatorcontrib>Giro, Elisa M.A.</creatorcontrib><creatorcontrib>Souza, Pedro P.C.</creatorcontrib><creatorcontrib>Hebling, Josimeri</creatorcontrib><creatorcontrib>de Souza Costa, Carlos A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Dental materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aranha, Andreza M.F.</au><au>Giro, Elisa M.A.</au><au>Souza, Pedro P.C.</au><au>Hebling, Josimeri</au><au>de Souza Costa, Carlos A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of curing regime on the cytotoxicity of resin-modified glass-ionomer lining cements applied to an odontoblast-cell line</atitle><jtitle>Dental materials</jtitle><addtitle>Dent Mater</addtitle><date>2006-09-01</date><risdate>2006</risdate><volume>22</volume><issue>9</issue><spage>864</spage><epage>869</epage><pages>864-869</pages><issn>0109-5641</issn><eissn>1879-0097</eissn><abstract>The aim of this in vitro study was to evaluate the cytotoxicity of resin-modified glass-ionomer lining cements submitted to different curing regimes and applied to an immortalized odontoblast-cell line (MDPC-23).
Forty round-shaped specimens of each experimental material (Fuji Lining LC and Vitrebond) were prepared. They were light-cured for the manufacturers’ recommended time (MRT
=
30
s), under-cured (0.5 MRT
=
15
s), over-cured (1.5 MRT
=
45
s) or allowed to dark cure (0 MRT). Sterilized filter papers soaked with either 5
μL of PBS or HEMA were used as negative and positive control, respectively. After placing the specimens individually in wells of 24-well dishes, odontoblast-like cells MDPC-23 (30,000
cells/cm
2) were plated in each well and incubated for 72
h in a humidified incubator at 37
°C with 5% CO
2 and 95% air. The cytotoxicity was evaluated by the cell metabolism (MTT assay) and cell morphology (SEM).
Fuji Lining LC was less cytotoxic than Vitrebond (
p
<
0.05) in all the experimental conditions. However, the cytotoxicity of Fuji Lining LC was noticeably increased in the absence of light-curing while the same was not observed for Vitrebond. The length of light-curing (15, 30 or 45
s) did not influence the toxicity of both lining materials when they were applied on the odontoblast-cell line MDPC-23.
The light-activation plays an important role in reducing the cytotoxicity of Fuji Lining LC. Following the manufacturer’ recommendation regarding the light-curing regime may prevent toxic effect to the pulp cells.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>16388848</pmid><doi>10.1016/j.dental.2005.11.015</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0109-5641 |
| ispartof | Dental materials, 2006-09, Vol.22 (9), p.864-869 |
| issn | 0109-5641 1879-0097 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_29515196 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Cell Line, Transformed Cell Shape - drug effects Coloring Agents - metabolism Curing regime Cytotoxicity Dental Cavity Lining Dentin-Bonding Agents - radiation effects Dentin-Bonding Agents - toxicity Glass Ionomer Cements - chemistry Glass Ionomer Cements - radiation effects Glass Ionomer Cements - toxicity Glass-ionomer cements HEMA Light Methacrylates - toxicity Mice Microscopy, Electron, Scanning Odontoblast Odontoblasts - drug effects Phase Transition Resins, Synthetic - radiation effects Resins, Synthetic - toxicity Tetrazolium Salts - metabolism |
| title | Effect of curing regime on the cytotoxicity of resin-modified glass-ionomer lining cements applied to an odontoblast-cell line |
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