Application of factorial and Doehlert designs for optimization of protopectinase production by a Geotrichum klebahnii strain
Two successive factorial designs followed by two response surface methodology were applied to optimize protopectinase production by Geotrichum klebahnii. Factorial designs were used to study the effect of 11 variables (mineral pool and pH) on enzyme production. Only pH and Fe 2+ had significant effe...
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| Veröffentlicht in: | Process biochemistry (1991) 2007-02, Vol.42 (2), p.175-179 |
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| container_title | Process biochemistry (1991) |
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| creator | Cavalitto, S.F. Mignone, C.F. |
| description | Two successive factorial designs followed by two response surface methodology were applied to optimize protopectinase production by
Geotrichum klebahnii. Factorial designs were used to study the effect of 11 variables (mineral pool and pH) on enzyme production. Only pH and Fe
2+ had significant effect on the protopectinase production in the conditions of the assay, the interaction between them not being significant. According to this result pH and Fe
2+ were multivariate according to a Doelhert design. The central points were pH 3.5 and Fe
2+ 1.8
μmol
L
−1. The results show a negative effect of pH and a positive (quadratic) effect of Fe
2+ on enzyme production. The second Doelhert design was centered at pH 3.3. A relative maximum was obtained at pH 2.8 and Fe
2+ 0.540
μmol
L
−1, where the enzyme activity obtained was 236
U/mL. This value is two times higher than the value reported elsewhere. |
| doi_str_mv | 10.1016/j.procbio.2006.07.031 |
| format | Article |
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Geotrichum klebahnii. Factorial designs were used to study the effect of 11 variables (mineral pool and pH) on enzyme production. Only pH and Fe
2+ had significant effect on the protopectinase production in the conditions of the assay, the interaction between them not being significant. According to this result pH and Fe
2+ were multivariate according to a Doelhert design. The central points were pH 3.5 and Fe
2+ 1.8
μmol
L
−1. The results show a negative effect of pH and a positive (quadratic) effect of Fe
2+ on enzyme production. The second Doelhert design was centered at pH 3.3. A relative maximum was obtained at pH 2.8 and Fe
2+ 0.540
μmol
L
−1, where the enzyme activity obtained was 236
U/mL. This value is two times higher than the value reported elsewhere.</description><identifier>ISSN: 1359-5113</identifier><identifier>EISSN: 1873-3298</identifier><identifier>DOI: 10.1016/j.procbio.2006.07.031</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>Doehlert design ; Enzyme production ; Geotrichum klebahnii ; Plackett–Burman design ; Protopectinase ; Trace elements</subject><ispartof>Process biochemistry (1991), 2007-02, Vol.42 (2), p.175-179</ispartof><rights>2006 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-ee1418dbce4e20aa5450beb6167d64b1f403aa23c1cba039b6841525f0eca0453</citedby><cites>FETCH-LOGICAL-c371t-ee1418dbce4e20aa5450beb6167d64b1f403aa23c1cba039b6841525f0eca0453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.procbio.2006.07.031$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids></links><search><creatorcontrib>Cavalitto, S.F.</creatorcontrib><creatorcontrib>Mignone, C.F.</creatorcontrib><title>Application of factorial and Doehlert designs for optimization of protopectinase production by a Geotrichum klebahnii strain</title><title>Process biochemistry (1991)</title><description>Two successive factorial designs followed by two response surface methodology were applied to optimize protopectinase production by
Geotrichum klebahnii. Factorial designs were used to study the effect of 11 variables (mineral pool and pH) on enzyme production. Only pH and Fe
2+ had significant effect on the protopectinase production in the conditions of the assay, the interaction between them not being significant. According to this result pH and Fe
2+ were multivariate according to a Doelhert design. The central points were pH 3.5 and Fe
2+ 1.8
μmol
L
−1. The results show a negative effect of pH and a positive (quadratic) effect of Fe
2+ on enzyme production. The second Doelhert design was centered at pH 3.3. A relative maximum was obtained at pH 2.8 and Fe
2+ 0.540
μmol
L
−1, where the enzyme activity obtained was 236
U/mL. This value is two times higher than the value reported elsewhere.</description><subject>Doehlert design</subject><subject>Enzyme production</subject><subject>Geotrichum klebahnii</subject><subject>Plackett–Burman design</subject><subject>Protopectinase</subject><subject>Trace elements</subject><issn>1359-5113</issn><issn>1873-3298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkE1P3DAQhiPUSlDoT0DyqbekM3GcbE4VouVDQuICZ8t2Juxss3FqeyuB-PH1sqhXTjOjed_5eIriHKFCwPb7plqCd5Z9VQO0FXQVSDwqTnDVyVLW_epTzqXqS4Uoj4svMW4gKxDhpHi9WJaJnUnsZ-FHMRqXfGAzCTMP4qen9UQhiYEiP81RjD4IvyTe8st_S16e_EIu8Wwi7cth596a9lkYcU0-BXbr3Vb8nsia9cwsYgqG57Pi82imSF_f42nxePXr4fKmvLu_vr28uCud7DCVRNjgarCOGqrBGNUosGRbbLuhbSyODUhjaunQWQOyt-2qQVWrEcgZaJQ8Lb4d5ubb_uwoJr3l6GiazEx-F3XdK-hrqLNQHYQu-BgDjXoJvDXhWSPoPWu90e-s9Z61hk5nktn34-Cj_MVfpqCjY5odDRwyGD14_mDCPy2tjh4</recordid><startdate>20070201</startdate><enddate>20070201</enddate><creator>Cavalitto, S.F.</creator><creator>Mignone, C.F.</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>L7M</scope></search><sort><creationdate>20070201</creationdate><title>Application of factorial and Doehlert designs for optimization of protopectinase production by a Geotrichum klebahnii strain</title><author>Cavalitto, S.F. ; Mignone, C.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-ee1418dbce4e20aa5450beb6167d64b1f403aa23c1cba039b6841525f0eca0453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Doehlert design</topic><topic>Enzyme production</topic><topic>Geotrichum klebahnii</topic><topic>Plackett–Burman design</topic><topic>Protopectinase</topic><topic>Trace elements</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cavalitto, S.F.</creatorcontrib><creatorcontrib>Mignone, C.F.</creatorcontrib><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Process biochemistry (1991)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cavalitto, S.F.</au><au>Mignone, C.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of factorial and Doehlert designs for optimization of protopectinase production by a Geotrichum klebahnii strain</atitle><jtitle>Process biochemistry (1991)</jtitle><date>2007-02-01</date><risdate>2007</risdate><volume>42</volume><issue>2</issue><spage>175</spage><epage>179</epage><pages>175-179</pages><issn>1359-5113</issn><eissn>1873-3298</eissn><abstract>Two successive factorial designs followed by two response surface methodology were applied to optimize protopectinase production by
Geotrichum klebahnii. Factorial designs were used to study the effect of 11 variables (mineral pool and pH) on enzyme production. Only pH and Fe
2+ had significant effect on the protopectinase production in the conditions of the assay, the interaction between them not being significant. According to this result pH and Fe
2+ were multivariate according to a Doelhert design. The central points were pH 3.5 and Fe
2+ 1.8
μmol
L
−1. The results show a negative effect of pH and a positive (quadratic) effect of Fe
2+ on enzyme production. The second Doelhert design was centered at pH 3.3. A relative maximum was obtained at pH 2.8 and Fe
2+ 0.540
μmol
L
−1, where the enzyme activity obtained was 236
U/mL. This value is two times higher than the value reported elsewhere.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.procbio.2006.07.031</doi><tpages>5</tpages></addata></record> |
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| ispartof | Process biochemistry (1991), 2007-02, Vol.42 (2), p.175-179 |
| issn | 1359-5113 1873-3298 |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | Doehlert design Enzyme production Geotrichum klebahnii Plackett–Burman design Protopectinase Trace elements |
| title | Application of factorial and Doehlert designs for optimization of protopectinase production by a Geotrichum klebahnii strain |
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