Inhibitory effect of phenolics extracted from sorghum genotypes on Aspergillus parasiticus (NRRL 2999) growth and aflatoxin production
The inhibitory activity of bioactive polyphenols present in six sorghum genotypes -- two red (AON 486 and IS 620), two yellow (LPJ and IS 17779) and two white (SPV 86 and SPV 462) varieties -- on Aspergillus parasiticus (NRRL 2999) growth and aflatoxin production was evaluated. In the first experime...
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| Veröffentlicht in: | Journal of the science of food and agriculture 2007-04, Vol.87 (6), p.1140-1148 |
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| creator | Ratnavathi, C.V Sashidhar, R.B |
| description | The inhibitory activity of bioactive polyphenols present in six sorghum genotypes -- two red (AON 486 and IS 620), two yellow (LPJ and IS 17779) and two white (SPV 86 and SPV 462) varieties -- on Aspergillus parasiticus (NRRL 2999) growth and aflatoxin production was evaluated. In the first experiment the production of aflatoxins in the six sorghum genotypes after removal of surface phenolics by acidic methanol treatment was studied and compared with that in untreated grains. Aflatoxin production was found to be fourfold higher in treated grains. The total phenols and bioactive polyphenols extracted by acidic methanol were quantified using the Folin-Denis method and the bovine serum albumin-benzidine conjugate procedure respectively. In the second experiment the effect of extracted sorghum phenolics under in vitro conditions on fungal growth and aflatoxin production was studied at two concentrations (0.01% and 0.1%) of phenolics. Extracted phenolics added to yeast extract sucrose (YES) medium at 0.1% concentration showed an inhibitory effect on aflatoxin production. At 0.01% phenolic concentration, aflatoxin production was minimal on day 3 after infection. At other time points the aflatoxin content was similar to that in the control. At 9 days after infection the fungal biomass in IS 620 was significantly lower than that in the control. At 0.1% phenolic concentration, aflatoxin production was minimal and the red genotype IS 620 showed maximum resistance. Fungal biomass was lowest at all growth stages in IS 620 as compared with the control. Polyphenol oxidase (PPO) activity was not detected in A. parasiticus grown on YES medium (control). PPO activity was not induced in A. parasiticus by the addition of phenolics to the liquid culture medium (no PPO activity was detected in the culture medium). The inhibitory activity of bioactive polyphenols could be attributed to the lack of PPO enzyme in this fungus. |
| doi_str_mv | 10.1002/jsfa.2827 |
| format | Article |
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In the first experiment the production of aflatoxins in the six sorghum genotypes after removal of surface phenolics by acidic methanol treatment was studied and compared with that in untreated grains. Aflatoxin production was found to be fourfold higher in treated grains. The total phenols and bioactive polyphenols extracted by acidic methanol were quantified using the Folin-Denis method and the bovine serum albumin-benzidine conjugate procedure respectively. In the second experiment the effect of extracted sorghum phenolics under in vitro conditions on fungal growth and aflatoxin production was studied at two concentrations (0.01% and 0.1%) of phenolics. Extracted phenolics added to yeast extract sucrose (YES) medium at 0.1% concentration showed an inhibitory effect on aflatoxin production. At 0.01% phenolic concentration, aflatoxin production was minimal on day 3 after infection. At other time points the aflatoxin content was similar to that in the control. At 9 days after infection the fungal biomass in IS 620 was significantly lower than that in the control. At 0.1% phenolic concentration, aflatoxin production was minimal and the red genotype IS 620 showed maximum resistance. Fungal biomass was lowest at all growth stages in IS 620 as compared with the control. Polyphenol oxidase (PPO) activity was not detected in A. parasiticus grown on YES medium (control). PPO activity was not induced in A. parasiticus by the addition of phenolics to the liquid culture medium (no PPO activity was detected in the culture medium). The inhibitory activity of bioactive polyphenols could be attributed to the lack of PPO enzyme in this fungus.</description><identifier>ISSN: 0022-5142</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.2827</identifier><identifier>CODEN: JSFAAE</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>aflatoxin ; Aflatoxins ; Aspergillus parasiticus ; Biological and medical sciences ; catechol oxidase ; Cereal and baking product industries ; Chemical compounds ; color ; culture media ; Enzymes ; food contamination ; Food industries ; Food science ; Food toxicology ; Fundamental and applied biological sciences. Psychology ; fungal biomass ; Fungi ; genotype ; Genotype & phenotype ; Genotypes ; grain sorghum ; Grasses ; in vitro studies ; mechanism of action ; Methanol ; microbial contamination ; microbial growth ; phenolics ; Phenols ; Plant growth ; polyphenol oxidase ; polyphenols ; Sorghum ; Sorghum bicolor ; Toxins ; Yeasts</subject><ispartof>Journal of the science of food and agriculture, 2007-04, Vol.87 (6), p.1140-1148</ispartof><rights>Copyright © 2007 Society of Chemical Industry</rights><rights>2007 INIST-CNRS</rights><rights>Copyright John Wiley and Sons, Limited Apr 30, 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4507-adeca54d6eb86b39d9669227a000057c5da06e3ccdc43e8c9843e865ef13cb8f3</citedby><cites>FETCH-LOGICAL-c4507-adeca54d6eb86b39d9669227a000057c5da06e3ccdc43e8c9843e865ef13cb8f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjsfa.2827$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjsfa.2827$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18671616$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Ratnavathi, C.V</creatorcontrib><creatorcontrib>Sashidhar, R.B</creatorcontrib><title>Inhibitory effect of phenolics extracted from sorghum genotypes on Aspergillus parasiticus (NRRL 2999) growth and aflatoxin production</title><title>Journal of the science of food and agriculture</title><addtitle>J. Sci. Food Agric</addtitle><description>The inhibitory activity of bioactive polyphenols present in six sorghum genotypes -- two red (AON 486 and IS 620), two yellow (LPJ and IS 17779) and two white (SPV 86 and SPV 462) varieties -- on Aspergillus parasiticus (NRRL 2999) growth and aflatoxin production was evaluated. In the first experiment the production of aflatoxins in the six sorghum genotypes after removal of surface phenolics by acidic methanol treatment was studied and compared with that in untreated grains. Aflatoxin production was found to be fourfold higher in treated grains. The total phenols and bioactive polyphenols extracted by acidic methanol were quantified using the Folin-Denis method and the bovine serum albumin-benzidine conjugate procedure respectively. In the second experiment the effect of extracted sorghum phenolics under in vitro conditions on fungal growth and aflatoxin production was studied at two concentrations (0.01% and 0.1%) of phenolics. Extracted phenolics added to yeast extract sucrose (YES) medium at 0.1% concentration showed an inhibitory effect on aflatoxin production. At 0.01% phenolic concentration, aflatoxin production was minimal on day 3 after infection. At other time points the aflatoxin content was similar to that in the control. At 9 days after infection the fungal biomass in IS 620 was significantly lower than that in the control. At 0.1% phenolic concentration, aflatoxin production was minimal and the red genotype IS 620 showed maximum resistance. Fungal biomass was lowest at all growth stages in IS 620 as compared with the control. Polyphenol oxidase (PPO) activity was not detected in A. parasiticus grown on YES medium (control). PPO activity was not induced in A. parasiticus by the addition of phenolics to the liquid culture medium (no PPO activity was detected in the culture medium). The inhibitory activity of bioactive polyphenols could be attributed to the lack of PPO enzyme in this fungus.</description><subject>aflatoxin</subject><subject>Aflatoxins</subject><subject>Aspergillus parasiticus</subject><subject>Biological and medical sciences</subject><subject>catechol oxidase</subject><subject>Cereal and baking product industries</subject><subject>Chemical compounds</subject><subject>color</subject><subject>culture media</subject><subject>Enzymes</subject><subject>food contamination</subject><subject>Food industries</subject><subject>Food science</subject><subject>Food toxicology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>fungal biomass</subject><subject>Fungi</subject><subject>genotype</subject><subject>Genotype & phenotype</subject><subject>Genotypes</subject><subject>grain sorghum</subject><subject>Grasses</subject><subject>in vitro studies</subject><subject>mechanism of action</subject><subject>Methanol</subject><subject>microbial contamination</subject><subject>microbial growth</subject><subject>phenolics</subject><subject>Phenols</subject><subject>Plant growth</subject><subject>polyphenol oxidase</subject><subject>polyphenols</subject><subject>Sorghum</subject><subject>Sorghum bicolor</subject><subject>Toxins</subject><subject>Yeasts</subject><issn>0022-5142</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkVFv0zAUhSMEEmXwwC_AQgKxh2y2kzjxYzfRMlSG1DF4tG4dO3VJ42A7WvsH-N04SgUSEuLpWrrfOb5HJ0leEnxBMKaXO6_hgla0fJTMCOZlijHBj5NZ3NG0IDl9mjzzfocx5pyxWfLzptuajQnWHZHSWsmArEb9VnW2NdIjdQgOZFA10s7ukbeu2Q571MR9OPbKI9uhue-Va0zbDh714MCbYGR8v7tdr1eIcs7PUePsQ9gi6GoEuoVgD6ZDvbP1IIOx3fPkiYbWqxeneZbcL95_uf6Qrj4vb67nq1TmBS5TqJWEIq-Z2lRsk_E6ZuCUlhDz4KKURQ2YqUzKWuaZqiSvxsEKpUkmN5XOzpK3k2_8-segfBB746VqW-iUHbygPKcZL8h_QcIrzDgrI_j6L3BnB9fFEIJSykqalTxC5xMknfXeKS16Z_bgjoJgMfYmxt7E2Ftk35wMwUtotYNOGv9HULGSMMIidzlxD6ZVx38bio93i_nJOZ0Uxgd1-K0A913EIGUhvt0uxdWnr0u-wGsx8q8mXoMV0Lh4xf0dxSTDuGI5oTz7BSOPwOY</recordid><startdate>20070430</startdate><enddate>20070430</enddate><creator>Ratnavathi, C.V</creator><creator>Sashidhar, R.B</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><general>John Wiley and Sons, Limited</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QL</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7ST</scope><scope>7T5</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7U7</scope></search><sort><creationdate>20070430</creationdate><title>Inhibitory effect of phenolics extracted from sorghum genotypes on Aspergillus parasiticus (NRRL 2999) growth and aflatoxin production</title><author>Ratnavathi, C.V ; Sashidhar, R.B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4507-adeca54d6eb86b39d9669227a000057c5da06e3ccdc43e8c9843e865ef13cb8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>aflatoxin</topic><topic>Aflatoxins</topic><topic>Aspergillus parasiticus</topic><topic>Biological and medical sciences</topic><topic>catechol oxidase</topic><topic>Cereal and baking product industries</topic><topic>Chemical compounds</topic><topic>color</topic><topic>culture media</topic><topic>Enzymes</topic><topic>food contamination</topic><topic>Food industries</topic><topic>Food science</topic><topic>Food toxicology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>fungal biomass</topic><topic>Fungi</topic><topic>genotype</topic><topic>Genotype & phenotype</topic><topic>Genotypes</topic><topic>grain sorghum</topic><topic>Grasses</topic><topic>in vitro studies</topic><topic>mechanism of action</topic><topic>Methanol</topic><topic>microbial contamination</topic><topic>microbial growth</topic><topic>phenolics</topic><topic>Phenols</topic><topic>Plant growth</topic><topic>polyphenol oxidase</topic><topic>polyphenols</topic><topic>Sorghum</topic><topic>Sorghum bicolor</topic><topic>Toxins</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ratnavathi, C.V</creatorcontrib><creatorcontrib>Sashidhar, R.B</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>Toxicology Abstracts</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ratnavathi, C.V</au><au>Sashidhar, R.B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory effect of phenolics extracted from sorghum genotypes on Aspergillus parasiticus (NRRL 2999) growth and aflatoxin production</atitle><jtitle>Journal of the science of food and agriculture</jtitle><addtitle>J. Sci. Food Agric</addtitle><date>2007-04-30</date><risdate>2007</risdate><volume>87</volume><issue>6</issue><spage>1140</spage><epage>1148</epage><pages>1140-1148</pages><issn>0022-5142</issn><eissn>1097-0010</eissn><coden>JSFAAE</coden><abstract>The inhibitory activity of bioactive polyphenols present in six sorghum genotypes -- two red (AON 486 and IS 620), two yellow (LPJ and IS 17779) and two white (SPV 86 and SPV 462) varieties -- on Aspergillus parasiticus (NRRL 2999) growth and aflatoxin production was evaluated. In the first experiment the production of aflatoxins in the six sorghum genotypes after removal of surface phenolics by acidic methanol treatment was studied and compared with that in untreated grains. Aflatoxin production was found to be fourfold higher in treated grains. The total phenols and bioactive polyphenols extracted by acidic methanol were quantified using the Folin-Denis method and the bovine serum albumin-benzidine conjugate procedure respectively. In the second experiment the effect of extracted sorghum phenolics under in vitro conditions on fungal growth and aflatoxin production was studied at two concentrations (0.01% and 0.1%) of phenolics. Extracted phenolics added to yeast extract sucrose (YES) medium at 0.1% concentration showed an inhibitory effect on aflatoxin production. At 0.01% phenolic concentration, aflatoxin production was minimal on day 3 after infection. At other time points the aflatoxin content was similar to that in the control. At 9 days after infection the fungal biomass in IS 620 was significantly lower than that in the control. At 0.1% phenolic concentration, aflatoxin production was minimal and the red genotype IS 620 showed maximum resistance. Fungal biomass was lowest at all growth stages in IS 620 as compared with the control. Polyphenol oxidase (PPO) activity was not detected in A. parasiticus grown on YES medium (control). PPO activity was not induced in A. parasiticus by the addition of phenolics to the liquid culture medium (no PPO activity was detected in the culture medium). The inhibitory activity of bioactive polyphenols could be attributed to the lack of PPO enzyme in this fungus.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/jsfa.2827</doi><tpages>9</tpages></addata></record> |
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| subjects | aflatoxin Aflatoxins Aspergillus parasiticus Biological and medical sciences catechol oxidase Cereal and baking product industries Chemical compounds color culture media Enzymes food contamination Food industries Food science Food toxicology Fundamental and applied biological sciences. Psychology fungal biomass Fungi genotype Genotype & phenotype Genotypes grain sorghum Grasses in vitro studies mechanism of action Methanol microbial contamination microbial growth phenolics Phenols Plant growth polyphenol oxidase polyphenols Sorghum Sorghum bicolor Toxins Yeasts |
| title | Inhibitory effect of phenolics extracted from sorghum genotypes on Aspergillus parasiticus (NRRL 2999) growth and aflatoxin production |
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