NANOJET as a chemical scalpel for accessing the internal 3D-structure of biological cells

NANOJET as a chemical scalpel for accessing the internal 3D-structure of biological cells

The aim of this work is to obtain information about the internal structure of biological objects (BOs) using the Nanonozzle Plasma Jet Microfabrication Tool (NANOJET). As BOs we used Dictyostelium amoeba. Our results show that the NANOJET allows removal of the cell plasma membrane with negligible da...

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Veröffentlicht in:Microelectronic engineering 2004-06, Vol.73, p.843-846
Hauptverfasser: Rabinovych, O., Pedrak, R., Rangelow, I.W., Ruehling, H., Maniak, M.
Format: Artikel
Sprache:eng
Schlagworte:
(Nano-)jet
Atomic-force microscopy
Critical-point-drying
Downstream plasma
Nozzle
Preparation of biological cells
Scanning electron microscopy
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Zusammenfassung:The aim of this work is to obtain information about the internal structure of biological objects (BOs) using the Nanonozzle Plasma Jet Microfabrication Tool (NANOJET). As BOs we used Dictyostelium amoeba. Our results show that the NANOJET allows removal of the cell plasma membrane with negligible damaging its internal structures. Using scanning electron microscopy, we are able to see the spherical and tubular organelles within the cell.
ISSN:0167-9317
1873-5568
DOI:10.1016/j.mee.2004.03.062