Microbial community and antimicrobial resistance niche differentiation in a multistage, surface flow constructed wetland
•First-time size-fractionated microbial communities have been analyzed in constructed wetlands.•Community analysis reveals microbial niche partitioning on taxonomic and metabolic level.•Constructed wetland effectively immobilized wastewater particle-associated bacteria and associated AMR load.•Free-...
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| Veröffentlicht in: | Water research (Oxford) 2024-05, Vol.254, p.121408-121408, Article 121408 |
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| creator | Bydalek, Franciszek Webster, Gordon Barden, Ruth Weightman, Andrew J. Kasprzyk-Hordern, Barbara Wenk, Jannis |
| description | •First-time size-fractionated microbial communities have been analyzed in constructed wetlands.•Community analysis reveals microbial niche partitioning on taxonomic and metabolic level.•Constructed wetland effectively immobilized wastewater particle-associated bacteria and associated AMR load.•Free-living bacteria can be a primary escape route of AMR from constructed wetlands.
Free-living (FL) and particulate-associated (PA) communities are distinct bacterioplankton lifestyles with different mobility and dissemination routes. Understanding spatio-temporal dynamics of PA and FL fractions will allow improvement to wastewater treatment processes including pathogen and AMR bacteria removal. In this study, PA, FL and sediment community composition and antimicrobial resistance gene (ARG; tetW, ermB, sul1, intI1) dynamics were investigated in a full-scale municipal wastewater free-water surface polishing constructed wetland. Taxonomic composition of PA and FL microbial communities shifted towards less diverse communities (Shannon, Chao1) at the CW effluent but retained a distinct fraction-specific composition. Wastewater treatment plant derived PA communities introduced the bulk of AMR load (70 %) into the CW. However, the FL fraction was responsible for exporting over 60 % of the effluent AMR load given its high mobility and the effective immobilization (1–3 log removal) of PA communities. Strong correlations (r2>0.8, p < 0.05) were observed between the FL fraction, tetW and emrB dynamics, and amplicon sequence variants (ASVs) of potentially pathogenic taxa, including Bacteroides, Enterobacteriaceae, Aeromonadaceae, and Lachnospiraceae. This study reveals niche differentiation of microbial communities and associated AMR in CWs and shows that free-living bacteria are a primary escape route of pathogenic and ARG load from CWs under low-flow hydraulic conditions.
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| doi_str_mv | 10.1016/j.watres.2024.121408 |
| format | Article |
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Free-living (FL) and particulate-associated (PA) communities are distinct bacterioplankton lifestyles with different mobility and dissemination routes. Understanding spatio-temporal dynamics of PA and FL fractions will allow improvement to wastewater treatment processes including pathogen and AMR bacteria removal. In this study, PA, FL and sediment community composition and antimicrobial resistance gene (ARG; tetW, ermB, sul1, intI1) dynamics were investigated in a full-scale municipal wastewater free-water surface polishing constructed wetland. Taxonomic composition of PA and FL microbial communities shifted towards less diverse communities (Shannon, Chao1) at the CW effluent but retained a distinct fraction-specific composition. Wastewater treatment plant derived PA communities introduced the bulk of AMR load (70 %) into the CW. However, the FL fraction was responsible for exporting over 60 % of the effluent AMR load given its high mobility and the effective immobilization (1–3 log removal) of PA communities. Strong correlations (r2>0.8, p < 0.05) were observed between the FL fraction, tetW and emrB dynamics, and amplicon sequence variants (ASVs) of potentially pathogenic taxa, including Bacteroides, Enterobacteriaceae, Aeromonadaceae, and Lachnospiraceae. This study reveals niche differentiation of microbial communities and associated AMR in CWs and shows that free-living bacteria are a primary escape route of pathogenic and ARG load from CWs under low-flow hydraulic conditions.
[Display omitted]</description><identifier>ISSN: 0043-1354</identifier><identifier>EISSN: 1879-2448</identifier><identifier>DOI: 10.1016/j.watres.2024.121408</identifier><identifier>PMID: 38442607</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Aeromonadaceae ; antibiotic resistance ; antibiotic resistance genes ; Antimicrobial resistance ; bacterioplankton ; Bacteroides ; community structure ; Constructed wetlands ; ecological differentiation ; Enterobacteriaceae ; Lachnospiraceae ; Microbial communities ; municipal wastewater ; overland flow ; Particle-associated ; Pathogens ; Planktonic free-living ; sediments ; taxonomy ; wastewater treatment ; water</subject><ispartof>Water research (Oxford), 2024-05, Vol.254, p.121408-121408, Article 121408</ispartof><rights>2024</rights><rights>Copyright © 2024. Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c390t-b0acbb8d9d53fc0baba8c5ae3eb6104e9e99e32d7f7d0c8bd9969e5fedbdcaa03</cites><orcidid>0000-0003-1603-5665 ; 0000-0001-9182-0407 ; 0000-0002-9530-7835 ; 0000-0002-6809-2875</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0043135424003105$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65308</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38442607$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bydalek, Franciszek</creatorcontrib><creatorcontrib>Webster, Gordon</creatorcontrib><creatorcontrib>Barden, Ruth</creatorcontrib><creatorcontrib>Weightman, Andrew J.</creatorcontrib><creatorcontrib>Kasprzyk-Hordern, Barbara</creatorcontrib><creatorcontrib>Wenk, Jannis</creatorcontrib><title>Microbial community and antimicrobial resistance niche differentiation in a multistage, surface flow constructed wetland</title><title>Water research (Oxford)</title><addtitle>Water Res</addtitle><description>•First-time size-fractionated microbial communities have been analyzed in constructed wetlands.•Community analysis reveals microbial niche partitioning on taxonomic and metabolic level.•Constructed wetland effectively immobilized wastewater particle-associated bacteria and associated AMR load.•Free-living bacteria can be a primary escape route of AMR from constructed wetlands.
Free-living (FL) and particulate-associated (PA) communities are distinct bacterioplankton lifestyles with different mobility and dissemination routes. Understanding spatio-temporal dynamics of PA and FL fractions will allow improvement to wastewater treatment processes including pathogen and AMR bacteria removal. In this study, PA, FL and sediment community composition and antimicrobial resistance gene (ARG; tetW, ermB, sul1, intI1) dynamics were investigated in a full-scale municipal wastewater free-water surface polishing constructed wetland. Taxonomic composition of PA and FL microbial communities shifted towards less diverse communities (Shannon, Chao1) at the CW effluent but retained a distinct fraction-specific composition. Wastewater treatment plant derived PA communities introduced the bulk of AMR load (70 %) into the CW. However, the FL fraction was responsible for exporting over 60 % of the effluent AMR load given its high mobility and the effective immobilization (1–3 log removal) of PA communities. Strong correlations (r2>0.8, p < 0.05) were observed between the FL fraction, tetW and emrB dynamics, and amplicon sequence variants (ASVs) of potentially pathogenic taxa, including Bacteroides, Enterobacteriaceae, Aeromonadaceae, and Lachnospiraceae. This study reveals niche differentiation of microbial communities and associated AMR in CWs and shows that free-living bacteria are a primary escape route of pathogenic and ARG load from CWs under low-flow hydraulic conditions.
[Display omitted]</description><subject>Aeromonadaceae</subject><subject>antibiotic resistance</subject><subject>antibiotic resistance genes</subject><subject>Antimicrobial resistance</subject><subject>bacterioplankton</subject><subject>Bacteroides</subject><subject>community structure</subject><subject>Constructed wetlands</subject><subject>ecological differentiation</subject><subject>Enterobacteriaceae</subject><subject>Lachnospiraceae</subject><subject>Microbial communities</subject><subject>municipal wastewater</subject><subject>overland flow</subject><subject>Particle-associated</subject><subject>Pathogens</subject><subject>Planktonic free-living</subject><subject>sediments</subject><subject>taxonomy</subject><subject>wastewater treatment</subject><subject>water</subject><issn>0043-1354</issn><issn>1879-2448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkU1PFTEUhhsjkSv6D4zp0gVz6dfMtBsSQkRJIG503fTjFHozH9h2vPDv7c0gS1k0XfQ5523eB6FPlGwpod3Zbrs3JUHeMsLEljIqiHyDNlT2qmFCyLdoQ4jgDeWtOEbvc94RQhjj6h065lII1pF-gx5vo0uzjWbAbh7HZYrlCZvJ11Pi-PJWc2IuZnKAp-juAfsYAiSokClxnnCcsMHjMpQDdgenOC8pmIqHYd7X1VMuaXEFPN5DGWrAB3QUzJDh4_N9gn5dff15-b25-fHt-vLipnFckdJYYpy10ivf8uCINdZI1xrgYDtKBChQCjjzfeg9cdJ6pToFbQBvvTOG8BP0Zd37kObfC-Six5gdDPUPMC9Zc9ryVspOqFdRprhksutEX1GxorWgnBME_ZDiaNKTpkQf9OidXvXogx696qljn58TFjuCfxn656MC5ysAtZI_EZLOLkKt3ccErmg_x_8n_AUv9Kdk</recordid><startdate>20240501</startdate><enddate>20240501</enddate><creator>Bydalek, Franciszek</creator><creator>Webster, Gordon</creator><creator>Barden, Ruth</creator><creator>Weightman, Andrew J.</creator><creator>Kasprzyk-Hordern, Barbara</creator><creator>Wenk, Jannis</creator><general>Elsevier Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0003-1603-5665</orcidid><orcidid>https://orcid.org/0000-0001-9182-0407</orcidid><orcidid>https://orcid.org/0000-0002-9530-7835</orcidid><orcidid>https://orcid.org/0000-0002-6809-2875</orcidid></search><sort><creationdate>20240501</creationdate><title>Microbial community and antimicrobial resistance niche differentiation in a multistage, surface flow constructed wetland</title><author>Bydalek, Franciszek ; 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Free-living (FL) and particulate-associated (PA) communities are distinct bacterioplankton lifestyles with different mobility and dissemination routes. Understanding spatio-temporal dynamics of PA and FL fractions will allow improvement to wastewater treatment processes including pathogen and AMR bacteria removal. In this study, PA, FL and sediment community composition and antimicrobial resistance gene (ARG; tetW, ermB, sul1, intI1) dynamics were investigated in a full-scale municipal wastewater free-water surface polishing constructed wetland. Taxonomic composition of PA and FL microbial communities shifted towards less diverse communities (Shannon, Chao1) at the CW effluent but retained a distinct fraction-specific composition. Wastewater treatment plant derived PA communities introduced the bulk of AMR load (70 %) into the CW. However, the FL fraction was responsible for exporting over 60 % of the effluent AMR load given its high mobility and the effective immobilization (1–3 log removal) of PA communities. Strong correlations (r2>0.8, p < 0.05) were observed between the FL fraction, tetW and emrB dynamics, and amplicon sequence variants (ASVs) of potentially pathogenic taxa, including Bacteroides, Enterobacteriaceae, Aeromonadaceae, and Lachnospiraceae. This study reveals niche differentiation of microbial communities and associated AMR in CWs and shows that free-living bacteria are a primary escape route of pathogenic and ARG load from CWs under low-flow hydraulic conditions.
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| subjects | Aeromonadaceae antibiotic resistance antibiotic resistance genes Antimicrobial resistance bacterioplankton Bacteroides community structure Constructed wetlands ecological differentiation Enterobacteriaceae Lachnospiraceae Microbial communities municipal wastewater overland flow Particle-associated Pathogens Planktonic free-living sediments taxonomy wastewater treatment water |
| title | Microbial community and antimicrobial resistance niche differentiation in a multistage, surface flow constructed wetland |
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