Role of induced nitric oxide synthases in orofacial nociception/discomfort after dental tooth bleaching with hydrogen peroxide
To evaluate the role of induced nitric oxide synthase (iNOS) in nociception/orofacial discomfort in rats submitted to tooth whitening with hydrogen peroxide (H₂O₂). Wistar rats were divided into three groups (n = 24/group): a sham group not submitted to whitening treatment, a saline group submitted...
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| Veröffentlicht in: | Archives of oral biology 2024-05, Vol.161, p.105937-105937, Article 105937 |
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| creator | Pinto, Marcílio Rodrigues da Silva Medeiros, Kirlya Isabel Maia, Letícia Menezes Coelho, Antonio Alexandre Negreiros Nunes Alves, Ana Paula Caetano, Caio Ferreira Freire Mesquita, Karine Cestaro de Barros Silva, Paulo Goberlânio Sousa, Fabricio Bitu |
| description | To evaluate the role of induced nitric oxide synthase (iNOS) in nociception/orofacial discomfort in rats submitted to tooth whitening with hydrogen peroxide (H₂O₂).
Wistar rats were divided into three groups (n = 24/group): a sham group not submitted to whitening treatment, a saline group submitted to whitening treatment, and a test group submitted to whitening treatment and blockade of iNOS with aminoguanidine 50 mg/kg/day. After 24 and 48 h, and 7 days, the animals were euthanized to collect trigeminal ganglia and maxillae to histomorphometric analysis (size of neuronal bodies and percentage of pulp area filled by vessels) and behavior/nociception (Grimace scales, scratching and biting counting, weight loss and nociception assay). ANOVA-1- or − 2-way tests were used (p |
| doi_str_mv | 10.1016/j.archoralbio.2024.105937 |
| format | Article |
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Wistar rats were divided into three groups (n = 24/group): a sham group not submitted to whitening treatment, a saline group submitted to whitening treatment, and a test group submitted to whitening treatment and blockade of iNOS with aminoguanidine 50 mg/kg/day. After 24 and 48 h, and 7 days, the animals were euthanized to collect trigeminal ganglia and maxillae to histomorphometric analysis (size of neuronal bodies and percentage of pulp area filled by vessels) and behavior/nociception (Grimace scales, scratching and biting counting, weight loss and nociception assay). ANOVA-1- or − 2-way tests were used (p < 0.05, GraphPadPrism 5.0).
The aminoguanidine-treated group showed a reduction in nociceptive threshold in the masseteric region (p < 0.001), Grimace scale scores (p < 0.001), number of scratching (p = 0.011) and body mass loss (p = 0.007). After 24 and 48 h of tooth bleaching, the saline group showed a significant increase in the mean area of the blood vessels (p = 0.020) and iNOS immunostaining in odontoblasts (p = 0.002) and non-odontoblasts cells (p = 0.025). Aminoguanidine reversed both increases. Tooth bleaching reduced the mean area of neuronal bodies, and aminoguanidine significantly reversed it (p = 0.019), but an increase in GFAP immunostaining in neuronal bodies did not reduce after seven-days or after aminoguanidine treatment (p = 0.003).
iNOS blockage by aminoguanidine plays an important role in nociception and orofacial discomfort by control of inflammation in dental pulp after tooth bleaching with hydrogen peroxide (H₂O₂) 35%.
•Dental tooth bleaching leads do behavior alterations and increase in nociception.•Dental tooth bleaching increases inducible iNOS in dental pulp and inflammation in trigeminal ganglia.•Blockage of iNOS reduces dental inflammation and behavior/nociception alterations, without impair in neuronal inflammation.</description><identifier>ISSN: 0003-9969</identifier><identifier>EISSN: 1879-1506</identifier><identifier>DOI: 10.1016/j.archoralbio.2024.105937</identifier><identifier>PMID: 38442471</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Pain. Tooth Bleaching. Aminoguanidine. Nitric oxide</subject><ispartof>Archives of oral biology, 2024-05, Vol.161, p.105937-105937, Article 105937</ispartof><rights>2024 Elsevier Ltd</rights><rights>Copyright © 2024 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c321t-1a6734c7cb69e5cc728e32c303ac8f1aed56d6f1e260800e6209694d54a6ba853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S000399692400058X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38442471$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pinto, Marcílio Rodrigues</creatorcontrib><creatorcontrib>da Silva Medeiros, Kirlya Isabel</creatorcontrib><creatorcontrib>Maia, Letícia Menezes</creatorcontrib><creatorcontrib>Coelho, Antonio Alexandre</creatorcontrib><creatorcontrib>Negreiros Nunes Alves, Ana Paula</creatorcontrib><creatorcontrib>Caetano, Caio Ferreira Freire</creatorcontrib><creatorcontrib>Mesquita, Karine Cestaro</creatorcontrib><creatorcontrib>de Barros Silva, Paulo Goberlânio</creatorcontrib><creatorcontrib>Sousa, Fabricio Bitu</creatorcontrib><title>Role of induced nitric oxide synthases in orofacial nociception/discomfort after dental tooth bleaching with hydrogen peroxide</title><title>Archives of oral biology</title><addtitle>Arch Oral Biol</addtitle><description>To evaluate the role of induced nitric oxide synthase (iNOS) in nociception/orofacial discomfort in rats submitted to tooth whitening with hydrogen peroxide (H₂O₂).
Wistar rats were divided into three groups (n = 24/group): a sham group not submitted to whitening treatment, a saline group submitted to whitening treatment, and a test group submitted to whitening treatment and blockade of iNOS with aminoguanidine 50 mg/kg/day. After 24 and 48 h, and 7 days, the animals were euthanized to collect trigeminal ganglia and maxillae to histomorphometric analysis (size of neuronal bodies and percentage of pulp area filled by vessels) and behavior/nociception (Grimace scales, scratching and biting counting, weight loss and nociception assay). ANOVA-1- or − 2-way tests were used (p < 0.05, GraphPadPrism 5.0).
The aminoguanidine-treated group showed a reduction in nociceptive threshold in the masseteric region (p < 0.001), Grimace scale scores (p < 0.001), number of scratching (p = 0.011) and body mass loss (p = 0.007). After 24 and 48 h of tooth bleaching, the saline group showed a significant increase in the mean area of the blood vessels (p = 0.020) and iNOS immunostaining in odontoblasts (p = 0.002) and non-odontoblasts cells (p = 0.025). Aminoguanidine reversed both increases. Tooth bleaching reduced the mean area of neuronal bodies, and aminoguanidine significantly reversed it (p = 0.019), but an increase in GFAP immunostaining in neuronal bodies did not reduce after seven-days or after aminoguanidine treatment (p = 0.003).
iNOS blockage by aminoguanidine plays an important role in nociception and orofacial discomfort by control of inflammation in dental pulp after tooth bleaching with hydrogen peroxide (H₂O₂) 35%.
•Dental tooth bleaching leads do behavior alterations and increase in nociception.•Dental tooth bleaching increases inducible iNOS in dental pulp and inflammation in trigeminal ganglia.•Blockage of iNOS reduces dental inflammation and behavior/nociception alterations, without impair in neuronal inflammation.</description><subject>Pain. Tooth Bleaching. Aminoguanidine. Nitric oxide</subject><issn>0003-9969</issn><issn>1879-1506</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqNkE1vEzEQhi0EoqHwF5C5cdnUH7ve3SOKKFSqhITgbDnj2a6jjSfYDjQXfjsuKYgjJ2vsZ_zOPIy9kWIthTRXu7VLMFNyyzbQWgnV1vtu1P0TtpJDPzayE-YpWwkhdDOOZrxgL3Le1bIzRj5nF3poW9X2csV-fqYFOU08RH8E9DyGkgJwug8eeT7FMruMuT5zSjQ5CG7hkSAAHkqgeOVDBtpPlAp3U8HEPcZSmUJUZr5d0MEc4h3_EWo5n3yiO4z8gOl3wkv2bHJLxleP5yX7ev3-y-Zjc_vpw83m3W0DWsnSSGd63UIPWzNiB9CrAbUCLbSDYZIOfWe8mSQqIwYh0ChRt2591zqzdUOnL9nb87-HRN-OmIvd17lxWVxEOmarRj2owai-r-h4RiFRzgkne0hh79LJSmEf9Nud_Ue_fdBvz_pr7-vHmON2j_5v5x_fFdicAazLfg-YbIaAsYoPCaFYT-E_Yn4B81WfNQ</recordid><startdate>202405</startdate><enddate>202405</enddate><creator>Pinto, Marcílio Rodrigues</creator><creator>da Silva Medeiros, Kirlya Isabel</creator><creator>Maia, Letícia Menezes</creator><creator>Coelho, Antonio Alexandre</creator><creator>Negreiros Nunes Alves, Ana Paula</creator><creator>Caetano, Caio Ferreira Freire</creator><creator>Mesquita, Karine Cestaro</creator><creator>de Barros Silva, Paulo Goberlânio</creator><creator>Sousa, Fabricio Bitu</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202405</creationdate><title>Role of induced nitric oxide synthases in orofacial nociception/discomfort after dental tooth bleaching with hydrogen peroxide</title><author>Pinto, Marcílio Rodrigues ; da Silva Medeiros, Kirlya Isabel ; Maia, Letícia Menezes ; Coelho, Antonio Alexandre ; Negreiros Nunes Alves, Ana Paula ; Caetano, Caio Ferreira Freire ; Mesquita, Karine Cestaro ; de Barros Silva, Paulo Goberlânio ; Sousa, Fabricio Bitu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c321t-1a6734c7cb69e5cc728e32c303ac8f1aed56d6f1e260800e6209694d54a6ba853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Pain. Tooth Bleaching. Aminoguanidine. Nitric oxide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pinto, Marcílio Rodrigues</creatorcontrib><creatorcontrib>da Silva Medeiros, Kirlya Isabel</creatorcontrib><creatorcontrib>Maia, Letícia Menezes</creatorcontrib><creatorcontrib>Coelho, Antonio Alexandre</creatorcontrib><creatorcontrib>Negreiros Nunes Alves, Ana Paula</creatorcontrib><creatorcontrib>Caetano, Caio Ferreira Freire</creatorcontrib><creatorcontrib>Mesquita, Karine Cestaro</creatorcontrib><creatorcontrib>de Barros Silva, Paulo Goberlânio</creatorcontrib><creatorcontrib>Sousa, Fabricio Bitu</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of oral biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pinto, Marcílio Rodrigues</au><au>da Silva Medeiros, Kirlya Isabel</au><au>Maia, Letícia Menezes</au><au>Coelho, Antonio Alexandre</au><au>Negreiros Nunes Alves, Ana Paula</au><au>Caetano, Caio Ferreira Freire</au><au>Mesquita, Karine Cestaro</au><au>de Barros Silva, Paulo Goberlânio</au><au>Sousa, Fabricio Bitu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of induced nitric oxide synthases in orofacial nociception/discomfort after dental tooth bleaching with hydrogen peroxide</atitle><jtitle>Archives of oral biology</jtitle><addtitle>Arch Oral Biol</addtitle><date>2024-05</date><risdate>2024</risdate><volume>161</volume><spage>105937</spage><epage>105937</epage><pages>105937-105937</pages><artnum>105937</artnum><issn>0003-9969</issn><eissn>1879-1506</eissn><abstract>To evaluate the role of induced nitric oxide synthase (iNOS) in nociception/orofacial discomfort in rats submitted to tooth whitening with hydrogen peroxide (H₂O₂).
Wistar rats were divided into three groups (n = 24/group): a sham group not submitted to whitening treatment, a saline group submitted to whitening treatment, and a test group submitted to whitening treatment and blockade of iNOS with aminoguanidine 50 mg/kg/day. After 24 and 48 h, and 7 days, the animals were euthanized to collect trigeminal ganglia and maxillae to histomorphometric analysis (size of neuronal bodies and percentage of pulp area filled by vessels) and behavior/nociception (Grimace scales, scratching and biting counting, weight loss and nociception assay). ANOVA-1- or − 2-way tests were used (p < 0.05, GraphPadPrism 5.0).
The aminoguanidine-treated group showed a reduction in nociceptive threshold in the masseteric region (p < 0.001), Grimace scale scores (p < 0.001), number of scratching (p = 0.011) and body mass loss (p = 0.007). After 24 and 48 h of tooth bleaching, the saline group showed a significant increase in the mean area of the blood vessels (p = 0.020) and iNOS immunostaining in odontoblasts (p = 0.002) and non-odontoblasts cells (p = 0.025). Aminoguanidine reversed both increases. Tooth bleaching reduced the mean area of neuronal bodies, and aminoguanidine significantly reversed it (p = 0.019), but an increase in GFAP immunostaining in neuronal bodies did not reduce after seven-days or after aminoguanidine treatment (p = 0.003).
iNOS blockage by aminoguanidine plays an important role in nociception and orofacial discomfort by control of inflammation in dental pulp after tooth bleaching with hydrogen peroxide (H₂O₂) 35%.
•Dental tooth bleaching leads do behavior alterations and increase in nociception.•Dental tooth bleaching increases inducible iNOS in dental pulp and inflammation in trigeminal ganglia.•Blockage of iNOS reduces dental inflammation and behavior/nociception alterations, without impair in neuronal inflammation.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>38442471</pmid><doi>10.1016/j.archoralbio.2024.105937</doi><tpages>1</tpages></addata></record> |
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| ispartof | Archives of oral biology, 2024-05, Vol.161, p.105937-105937, Article 105937 |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Pain. Tooth Bleaching. Aminoguanidine. Nitric oxide |
| title | Role of induced nitric oxide synthases in orofacial nociception/discomfort after dental tooth bleaching with hydrogen peroxide |
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