Point-of-care detection of Monkeypox virus clades using high-performance upconversion nanoparticle-based lateral flow assay
There is an urgent need for a point-of-care testing (POCT) method in developing and underserved regions to distinguish between two Monkeypox virus (MPXV) clades, given their varying transmissibility and clinical manifestations. In this paper, we target the specific complement protein gene fragment o...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2024-04, Vol.191 (4), p.177, Article 177 |
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| container_title | Mikrochimica acta (1966) |
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| creator | Jin, Birui Ma, Chuan Zhang, Chuyao Yin, Huiling Zhao, Guoxu Hu, Jie Li, Zedong |
| description | There is an urgent need for a point-of-care testing (POCT) method in developing and underserved regions to distinguish between two Monkeypox virus (MPXV) clades, given their varying transmissibility and clinical manifestations. In this paper, we target the specific complement protein gene fragment of two MPXV clades and construct a high-performance upconversion nanoparticles–based lateral flow assay (UCNPs-based LFA) with double T-lines and a shared C-line. This enables qualitative and quantitative dual-mode detection when combined with a smartphone and a benchtop fluorescence analyzer. The developed LFA exhibits stable performance, convenient operation, rapid readout (within 8 min), and a much lower limit of detection (LOD) (~ pM level) compared to existing POCT methods. The proposed detection platform demonstrates significant potential for pathogen diagnosis using a POCT approach.
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| doi_str_mv | 10.1007/s00604-024-06241-3 |
| format | Article |
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Graphical Abstract</description><subject>Analytical Chemistry</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Limit of Detection</subject><subject>Microengineering</subject><subject>Monkeypox virus</subject><subject>Mpox</subject><subject>Mpox (monkeypox)</subject><subject>Nanochemistry</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Original Paper</subject><subject>Point-of-Care Systems</subject><subject>Point-of-Care Testing</subject><subject>Upconversion</subject><subject>Viruses</subject><issn>0026-3672</issn><issn>1436-5073</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1PFTEUhhuigSv6B1iYJm7cVPs17bA0BNEEowv2Tac9cxmY2w49M8ANf97CRUlcuGjOos_7npM8hBwJ_klwbj8j54ZrxmV9RmrB1B5ZCa0Ma7hVr8iKc2mYMlYekDeIV5wLW7l9cqBarYVp9Yo8_MpDmlnuWfAFaIQZwjzkRHNPf-R0Ddsp39PboSxIw-gjIF1wSGt6Oawv2QSlz2XjUwC6TCGnWyj4mE4-5cmXeQgjsM4jRDr6GYofaT_mO-oR_fYted37EeHd8zwkF19PL06-sfOfZ99PvpyzoGwzMyOFDm0jIiiv-tAILUMXouRdhD5qKzvdtiaCUN1xA14b03kVeQNWxoZrdUg-7mqnkm8WwNltBgwwjj5BXtDJY2Utb6xsK_rhH_QqLyXV456oVkgrZaXkjgolIxbo3VSGjS9bJ7h7NON2Zlw1457MOFVD75-rl24D8W_kj4oKqB2A9Sutobzs_k_tb2Phm0c</recordid><startdate>20240401</startdate><enddate>20240401</enddate><creator>Jin, Birui</creator><creator>Ma, Chuan</creator><creator>Zhang, Chuyao</creator><creator>Yin, Huiling</creator><creator>Zhao, Guoxu</creator><creator>Hu, Jie</creator><creator>Li, Zedong</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20240401</creationdate><title>Point-of-care detection of Monkeypox virus clades using high-performance upconversion nanoparticle-based lateral flow assay</title><author>Jin, Birui ; Ma, Chuan ; Zhang, Chuyao ; Yin, Huiling ; Zhao, Guoxu ; Hu, Jie ; Li, Zedong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-6214c851de3a3fc5142cbcd20bdefd472b4886de13b95ea466ba3d05e72d5043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Analytical Chemistry</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Limit of Detection</topic><topic>Microengineering</topic><topic>Monkeypox virus</topic><topic>Mpox</topic><topic>Mpox (monkeypox)</topic><topic>Nanochemistry</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Original Paper</topic><topic>Point-of-Care Systems</topic><topic>Point-of-Care Testing</topic><topic>Upconversion</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jin, Birui</creatorcontrib><creatorcontrib>Ma, Chuan</creatorcontrib><creatorcontrib>Zhang, Chuyao</creatorcontrib><creatorcontrib>Yin, Huiling</creatorcontrib><creatorcontrib>Zhao, Guoxu</creatorcontrib><creatorcontrib>Hu, Jie</creatorcontrib><creatorcontrib>Li, Zedong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jin, Birui</au><au>Ma, Chuan</au><au>Zhang, Chuyao</au><au>Yin, Huiling</au><au>Zhao, Guoxu</au><au>Hu, Jie</au><au>Li, Zedong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Point-of-care detection of Monkeypox virus clades using high-performance upconversion nanoparticle-based lateral flow assay</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2024-04-01</date><risdate>2024</risdate><volume>191</volume><issue>4</issue><spage>177</spage><pages>177-</pages><artnum>177</artnum><issn>0026-3672</issn><issn>1436-5073</issn><eissn>1436-5073</eissn><abstract>There is an urgent need for a point-of-care testing (POCT) method in developing and underserved regions to distinguish between two Monkeypox virus (MPXV) clades, given their varying transmissibility and clinical manifestations. In this paper, we target the specific complement protein gene fragment of two MPXV clades and construct a high-performance upconversion nanoparticles–based lateral flow assay (UCNPs-based LFA) with double T-lines and a shared C-line. This enables qualitative and quantitative dual-mode detection when combined with a smartphone and a benchtop fluorescence analyzer. The developed LFA exhibits stable performance, convenient operation, rapid readout (within 8 min), and a much lower limit of detection (LOD) (~ pM level) compared to existing POCT methods. The proposed detection platform demonstrates significant potential for pathogen diagnosis using a POCT approach.
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| subjects | Analytical Chemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Limit of Detection Microengineering Monkeypox virus Mpox Mpox (monkeypox) Nanochemistry Nanoparticles Nanotechnology Original Paper Point-of-Care Systems Point-of-Care Testing Upconversion Viruses |
| title | Point-of-care detection of Monkeypox virus clades using high-performance upconversion nanoparticle-based lateral flow assay |
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