Probing cell metabolism using the two-photon excitation autofluorescence lifetime of tryptophan

Compared to intensity detection, fluorescence lifetime has the advantage of being unaffected by variations in excitation intensity, fluorophore concentration, or attenuation due to biological absorption and scattering. In this Letter, to the best of our knowledge, we present the use of the two-photo...

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Veröffentlicht in:	Optics letters 2024-03, Vol.49 (5), p.1105-1108
Hauptverfasser:	Wu, Ting, Liao, Jiuling, Xiang, Feng, Yu, Jia, Huo, Yinwen, Gao, Yufeng, Li, Hui, Zheng, Wei
Format:	Artikel
Sprache:	eng
Schlagworte:	Apoptosis Carbonyls Excitation Fluorescence HeLa Cells Humans Molecular conformation Photons Tryptophan Tryptophan - chemistry Tumors
Online-Zugang:	Volltext
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