A rapid and low-cost platform for detection of bacterial based on microchamber PCR microfluidic chip
Polymerase chain reaction (PCR) has been considered as the gold standard for detecting nucleic acids. The simple PCR system is of great significance for medical applications in remote areas, especially for the developing countries. Herein, we proposed a low-cost self-assembled platform for microcham...
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| Veröffentlicht in: | Biomedical microdevices 2024-06, Vol.26 (2), p.20, Article 20 |
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| creator | Li, Zhenqing Ma, Xiaolu Zhang, Zhen Wang, Xiaoyang Yang, Bo Yang, Jing Zeng, Yuan Yuan, Xujun Zhang, Dawei Yamaguchi, Yoshinori |
| description | Polymerase chain reaction (PCR) has been considered as the gold standard for detecting nucleic acids. The simple PCR system is of great significance for medical applications in remote areas, especially for the developing countries. Herein, we proposed a low-cost self-assembled platform for microchamber PCR. The working principle is rotating the chamber PCR microfluidic chip between two heaters with fixed temperature to solve the problem of low temperature variation rate. The system consists of two temperature controllers, a screw slide rail, a chamber array microfluidic chip and a self-built software. Such a system can be constructed at a cost of about US$60. The micro chamber PCR can be finished by rotating the microfluidic chip between two heaters with fixed temperature. Results demonstrated that the sensitivity of the temperature controller is 0.1℃. The relative error of the duration for the microfluidic chip was 0.02 s. Finally, we successfully finished amplification of the target gene of
Porphyromonas gingivalis
in the chamber PCR microfluidic chip within 35 min and on-site detection of its PCR products by fluorescence. The chip consisted of 3200 cylindrical chambers. The volume of reagent in each volume is as low as 0.628 nL. This work provides an effective method to reduce the amplification time required for micro chamber PCR. |
| doi_str_mv | 10.1007/s10544-024-00699-x |
| format | Article |
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Porphyromonas gingivalis
in the chamber PCR microfluidic chip within 35 min and on-site detection of its PCR products by fluorescence. The chip consisted of 3200 cylindrical chambers. The volume of reagent in each volume is as low as 0.628 nL. This work provides an effective method to reduce the amplification time required for micro chamber PCR.</description><identifier>ISSN: 1387-2176</identifier><identifier>ISSN: 1572-8781</identifier><identifier>EISSN: 1572-8781</identifier><identifier>DOI: 10.1007/s10544-024-00699-x</identifier><identifier>PMID: 38430318</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Amplification ; Biological and Medical Physics ; Biomedical Engineering and Bioengineering ; Biophysics ; Cylindrical chambers ; Developing countries ; Engineering ; Engineering Fluid Dynamics ; LDCs ; Low cost ; Low temperature ; Microfluidics ; Microfluidics - methods ; Nanotechnology ; Nucleic acids ; Oligonucleotide Array Sequence Analysis - methods ; Pathogens ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Reagents ; Rotation ; Self-assembly ; Silicon wafers ; Temperature ; Temperature control</subject><ispartof>Biomedical microdevices, 2024-06, Vol.26 (2), p.20, Article 20</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-f774c12454837f03d8b386bdb9c30670b8eee9c558fc5fd22456b788ec3b6ff13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10544-024-00699-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10544-024-00699-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38430318$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Zhenqing</creatorcontrib><creatorcontrib>Ma, Xiaolu</creatorcontrib><creatorcontrib>Zhang, Zhen</creatorcontrib><creatorcontrib>Wang, Xiaoyang</creatorcontrib><creatorcontrib>Yang, Bo</creatorcontrib><creatorcontrib>Yang, Jing</creatorcontrib><creatorcontrib>Zeng, Yuan</creatorcontrib><creatorcontrib>Yuan, Xujun</creatorcontrib><creatorcontrib>Zhang, Dawei</creatorcontrib><creatorcontrib>Yamaguchi, Yoshinori</creatorcontrib><title>A rapid and low-cost platform for detection of bacterial based on microchamber PCR microfluidic chip</title><title>Biomedical microdevices</title><addtitle>Biomed Microdevices</addtitle><addtitle>Biomed Microdevices</addtitle><description>Polymerase chain reaction (PCR) has been considered as the gold standard for detecting nucleic acids. The simple PCR system is of great significance for medical applications in remote areas, especially for the developing countries. Herein, we proposed a low-cost self-assembled platform for microchamber PCR. The working principle is rotating the chamber PCR microfluidic chip between two heaters with fixed temperature to solve the problem of low temperature variation rate. The system consists of two temperature controllers, a screw slide rail, a chamber array microfluidic chip and a self-built software. Such a system can be constructed at a cost of about US$60. The micro chamber PCR can be finished by rotating the microfluidic chip between two heaters with fixed temperature. Results demonstrated that the sensitivity of the temperature controller is 0.1℃. The relative error of the duration for the microfluidic chip was 0.02 s. Finally, we successfully finished amplification of the target gene of
Porphyromonas gingivalis
in the chamber PCR microfluidic chip within 35 min and on-site detection of its PCR products by fluorescence. The chip consisted of 3200 cylindrical chambers. The volume of reagent in each volume is as low as 0.628 nL. This work provides an effective method to reduce the amplification time required for micro chamber PCR.</description><subject>Amplification</subject><subject>Biological and Medical Physics</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biophysics</subject><subject>Cylindrical chambers</subject><subject>Developing countries</subject><subject>Engineering</subject><subject>Engineering Fluid Dynamics</subject><subject>LDCs</subject><subject>Low cost</subject><subject>Low temperature</subject><subject>Microfluidics</subject><subject>Microfluidics - methods</subject><subject>Nanotechnology</subject><subject>Nucleic acids</subject><subject>Oligonucleotide Array Sequence Analysis - methods</subject><subject>Pathogens</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Reagents</subject><subject>Rotation</subject><subject>Self-assembly</subject><subject>Silicon wafers</subject><subject>Temperature</subject><subject>Temperature control</subject><issn>1387-2176</issn><issn>1572-8781</issn><issn>1572-8781</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1P3DAQhq2qqCzb_oEeKktceknxR2I7x9WqLUgrgRCcLX-yRkmc2omg_x4vgSJx6MH2aPzMO_a8AHzF6AdGiJ9ljJq6rhApC7G2rR4_gBVuOKkEF_hjiangFcGcHYOTnO8Rwi1j7BM4pqKmiGKxAnYDkxqDhWqwsIsPlYl5gmOnJh9TD8sGrZucmUIcYPRQKzO5FFRXouwsLNk-mBTNXvXaJXi1vV4SvpuDDQaafRg_gyOvuuy-vJxrcPvr5832vNpd_r7YbnaVoYRNlee8NpjUTS0o94haoalg2urWUMQ40sI515qmEd403pJCMs2FcIZq5j2ma_B90R1T_DO7PMk-ZOO6Tg0uzlmSltaENwKzgp6-Q-_jnIbyugN1mA8vE1oDslDlQzkn5-WYQq_SX4mRPHggFw9k8UA-eyAfS9G3F-lZ987-K3kdegHoAuRyNdy59Nb7P7JP8m2R9Q</recordid><startdate>20240601</startdate><enddate>20240601</enddate><creator>Li, Zhenqing</creator><creator>Ma, Xiaolu</creator><creator>Zhang, Zhen</creator><creator>Wang, Xiaoyang</creator><creator>Yang, Bo</creator><creator>Yang, Jing</creator><creator>Zeng, Yuan</creator><creator>Yuan, Xujun</creator><creator>Zhang, Dawei</creator><creator>Yamaguchi, Yoshinori</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SP</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>L7M</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20240601</creationdate><title>A rapid and low-cost platform for detection of bacterial based on microchamber PCR microfluidic chip</title><author>Li, Zhenqing ; Ma, Xiaolu ; Zhang, Zhen ; Wang, Xiaoyang ; Yang, Bo ; Yang, Jing ; Zeng, Yuan ; Yuan, Xujun ; Zhang, Dawei ; Yamaguchi, Yoshinori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-f774c12454837f03d8b386bdb9c30670b8eee9c558fc5fd22456b788ec3b6ff13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Amplification</topic><topic>Biological and Medical Physics</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biophysics</topic><topic>Cylindrical chambers</topic><topic>Developing countries</topic><topic>Engineering</topic><topic>Engineering Fluid Dynamics</topic><topic>LDCs</topic><topic>Low cost</topic><topic>Low temperature</topic><topic>Microfluidics</topic><topic>Microfluidics - methods</topic><topic>Nanotechnology</topic><topic>Nucleic acids</topic><topic>Oligonucleotide Array Sequence Analysis - methods</topic><topic>Pathogens</topic><topic>Polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Reagents</topic><topic>Rotation</topic><topic>Self-assembly</topic><topic>Silicon wafers</topic><topic>Temperature</topic><topic>Temperature control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Zhenqing</creatorcontrib><creatorcontrib>Ma, Xiaolu</creatorcontrib><creatorcontrib>Zhang, Zhen</creatorcontrib><creatorcontrib>Wang, Xiaoyang</creatorcontrib><creatorcontrib>Yang, Bo</creatorcontrib><creatorcontrib>Yang, Jing</creatorcontrib><creatorcontrib>Zeng, Yuan</creatorcontrib><creatorcontrib>Yuan, Xujun</creatorcontrib><creatorcontrib>Zhang, Dawei</creatorcontrib><creatorcontrib>Yamaguchi, Yoshinori</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical microdevices</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Zhenqing</au><au>Ma, Xiaolu</au><au>Zhang, Zhen</au><au>Wang, Xiaoyang</au><au>Yang, Bo</au><au>Yang, Jing</au><au>Zeng, Yuan</au><au>Yuan, Xujun</au><au>Zhang, Dawei</au><au>Yamaguchi, Yoshinori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid and low-cost platform for detection of bacterial based on microchamber PCR microfluidic chip</atitle><jtitle>Biomedical microdevices</jtitle><stitle>Biomed Microdevices</stitle><addtitle>Biomed Microdevices</addtitle><date>2024-06-01</date><risdate>2024</risdate><volume>26</volume><issue>2</issue><spage>20</spage><pages>20-</pages><artnum>20</artnum><issn>1387-2176</issn><issn>1572-8781</issn><eissn>1572-8781</eissn><abstract>Polymerase chain reaction (PCR) has been considered as the gold standard for detecting nucleic acids. The simple PCR system is of great significance for medical applications in remote areas, especially for the developing countries. Herein, we proposed a low-cost self-assembled platform for microchamber PCR. The working principle is rotating the chamber PCR microfluidic chip between two heaters with fixed temperature to solve the problem of low temperature variation rate. The system consists of two temperature controllers, a screw slide rail, a chamber array microfluidic chip and a self-built software. Such a system can be constructed at a cost of about US$60. The micro chamber PCR can be finished by rotating the microfluidic chip between two heaters with fixed temperature. Results demonstrated that the sensitivity of the temperature controller is 0.1℃. The relative error of the duration for the microfluidic chip was 0.02 s. Finally, we successfully finished amplification of the target gene of
Porphyromonas gingivalis
in the chamber PCR microfluidic chip within 35 min and on-site detection of its PCR products by fluorescence. The chip consisted of 3200 cylindrical chambers. The volume of reagent in each volume is as low as 0.628 nL. This work provides an effective method to reduce the amplification time required for micro chamber PCR.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>38430318</pmid><doi>10.1007/s10544-024-00699-x</doi></addata></record> |
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| ispartof | Biomedical microdevices, 2024-06, Vol.26 (2), p.20, Article 20 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Amplification Biological and Medical Physics Biomedical Engineering and Bioengineering Biophysics Cylindrical chambers Developing countries Engineering Engineering Fluid Dynamics LDCs Low cost Low temperature Microfluidics Microfluidics - methods Nanotechnology Nucleic acids Oligonucleotide Array Sequence Analysis - methods Pathogens Polymerase chain reaction Polymerase Chain Reaction - methods Reagents Rotation Self-assembly Silicon wafers Temperature Temperature control |
| title | A rapid and low-cost platform for detection of bacterial based on microchamber PCR microfluidic chip |
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