bmp-2 Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration
Bone morphogenetic protein-2 ( ) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the gene through in vivo electroporation. Natural bone tissues with skeletal muscles can be considered potential candidates for bi...
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| Veröffentlicht in: | Materials 2024-02, Vol.17 (4), p.880 |
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| creator | Kawai, Mariko Yamamoto Yoshida, Takeshi Kato, Tomoki Watanabe, Takuma Kashiwagi, Marina Yamanaka, Shigeki Yamamoto, Hiromitsu Nagahiro, Shigeki Iwamoto, Tsutomu Masud, Khan Aoki, Kazuhiro Ohura, Kiyoshi Nakao, Kazumasa |
| description | Bone morphogenetic protein-2 (
) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the
gene through in vivo electroporation. Natural bone tissues with skeletal muscles can be considered potential candidates for biomaterials. However, our previous system using plate-type electrodes did not achieve a 100% success rate in inducing bone tissues in skeletal muscles. In this study, we aimed to enhance the efficiency of bone tissue formation in skeletal muscles by using a non-viral
gene expression plasmid vector (pCAGGS-
) and needle-type electrodes.
We injected the
gene with pCAGGS-
into the skeletal muscles of rats' legs and immediately placed needle-type electrodes there. Skeletal tissues were then observed on the 21st day after gene transfer using soft X-ray and histological analyses.
The use of needle-type electrodes resulted in a 100% success rate in inducing bone tissues in skeletal muscles. In contrast, the plate-type electrodes only exhibited a 33% success rate. Thus, needle-type electrodes can be more efficient and reliable for transferring the
gene to skeletal muscles, making them potential biomaterials for repairing bone defects. |
| doi_str_mv | 10.3390/ma17040880 |
| format | Article |
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) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the
gene through in vivo electroporation. Natural bone tissues with skeletal muscles can be considered potential candidates for biomaterials. However, our previous system using plate-type electrodes did not achieve a 100% success rate in inducing bone tissues in skeletal muscles. In this study, we aimed to enhance the efficiency of bone tissue formation in skeletal muscles by using a non-viral
gene expression plasmid vector (pCAGGS-
) and needle-type electrodes.
We injected the
gene with pCAGGS-
into the skeletal muscles of rats' legs and immediately placed needle-type electrodes there. Skeletal tissues were then observed on the 21st day after gene transfer using soft X-ray and histological analyses.
The use of needle-type electrodes resulted in a 100% success rate in inducing bone tissues in skeletal muscles. In contrast, the plate-type electrodes only exhibited a 33% success rate. Thus, needle-type electrodes can be more efficient and reliable for transferring the
gene to skeletal muscles, making them potential biomaterials for repairing bone defects.</description><identifier>ISSN: 1996-1944</identifier><identifier>EISSN: 1996-1944</identifier><identifier>DOI: 10.3390/ma17040880</identifier><identifier>PMID: 38399131</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Biomedical materials ; Bones ; Calcification ; Efficiency ; Electric fields ; Electrodes ; Electroporation ; Fractures ; Gene expression ; Gene therapy ; Muscles ; Plasmids ; Regeneration (physiology) ; Soft x rays ; Stainless steel ; Vectors (Biology)</subject><ispartof>Materials, 2024-02, Vol.17 (4), p.880</ispartof><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c310t-7fe8e3a7355a9fcad6c3002e04f2584ae677a5a92efd9d6f9234e02d89879933</cites><orcidid>0000-0003-3306-3238 ; 0000-0001-5432-851X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38399131$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kawai, Mariko Yamamoto</creatorcontrib><creatorcontrib>Yoshida, Takeshi</creatorcontrib><creatorcontrib>Kato, Tomoki</creatorcontrib><creatorcontrib>Watanabe, Takuma</creatorcontrib><creatorcontrib>Kashiwagi, Marina</creatorcontrib><creatorcontrib>Yamanaka, Shigeki</creatorcontrib><creatorcontrib>Yamamoto, Hiromitsu</creatorcontrib><creatorcontrib>Nagahiro, Shigeki</creatorcontrib><creatorcontrib>Iwamoto, Tsutomu</creatorcontrib><creatorcontrib>Masud, Khan</creatorcontrib><creatorcontrib>Aoki, Kazuhiro</creatorcontrib><creatorcontrib>Ohura, Kiyoshi</creatorcontrib><creatorcontrib>Nakao, Kazumasa</creatorcontrib><title>bmp-2 Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration</title><title>Materials</title><addtitle>Materials (Basel)</addtitle><description>Bone morphogenetic protein-2 (
) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the
gene through in vivo electroporation. Natural bone tissues with skeletal muscles can be considered potential candidates for biomaterials. However, our previous system using plate-type electrodes did not achieve a 100% success rate in inducing bone tissues in skeletal muscles. In this study, we aimed to enhance the efficiency of bone tissue formation in skeletal muscles by using a non-viral
gene expression plasmid vector (pCAGGS-
) and needle-type electrodes.
We injected the
gene with pCAGGS-
into the skeletal muscles of rats' legs and immediately placed needle-type electrodes there. Skeletal tissues were then observed on the 21st day after gene transfer using soft X-ray and histological analyses.
The use of needle-type electrodes resulted in a 100% success rate in inducing bone tissues in skeletal muscles. In contrast, the plate-type electrodes only exhibited a 33% success rate. Thus, needle-type electrodes can be more efficient and reliable for transferring the
gene to skeletal muscles, making them potential biomaterials for repairing bone defects.</description><subject>Biomedical materials</subject><subject>Bones</subject><subject>Calcification</subject><subject>Efficiency</subject><subject>Electric fields</subject><subject>Electrodes</subject><subject>Electroporation</subject><subject>Fractures</subject><subject>Gene expression</subject><subject>Gene therapy</subject><subject>Muscles</subject><subject>Plasmids</subject><subject>Regeneration (physiology)</subject><subject>Soft x rays</subject><subject>Stainless steel</subject><subject>Vectors (Biology)</subject><issn>1996-1944</issn><issn>1996-1944</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkU1LxDAQhoMorqgXf4AEvIhQTTLdtjm6sn7AqqB7L9lmotG0WZMW8eZPN7J-4Vxm4H14Z5iXkD3OjgEkO2kVL1nOqoqtkS0uZZFxmefrf-YR2Y3xiaUC4JWQm2QEFUjJgW-R90W7zAS9wA6zeVBdNBgCanr_jA575ej1EBuHkb7a_pHeIGqXwLcl0qnDpg9eJ01FOjXGNha7nqpO0zt0Vi0c0on1reoxWOUiNT7Qie8wyQ9pX1C99d0O2TBJxN2vvk3m59P52WU2u724OjudZQ1w1melwQpBlTAeK2kapYsGGBPIciPGVa6wKEuVJIFGS10YKSBHJnQlq1JKgG1yuLJdBv8yYOzr1sYGnVMd-iHWQoLIoUgPSujBP_TJD6FLx31SnEHOoUzU0Ypqgo8xoKmXwbYqvNWc1Z_J1L_JJHj_y3JYtKh_0O8c4APzIYhK</recordid><startdate>20240214</startdate><enddate>20240214</enddate><creator>Kawai, Mariko Yamamoto</creator><creator>Yoshida, Takeshi</creator><creator>Kato, Tomoki</creator><creator>Watanabe, Takuma</creator><creator>Kashiwagi, Marina</creator><creator>Yamanaka, Shigeki</creator><creator>Yamamoto, Hiromitsu</creator><creator>Nagahiro, Shigeki</creator><creator>Iwamoto, Tsutomu</creator><creator>Masud, Khan</creator><creator>Aoki, Kazuhiro</creator><creator>Ohura, Kiyoshi</creator><creator>Nakao, Kazumasa</creator><general>MDPI AG</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3306-3238</orcidid><orcidid>https://orcid.org/0000-0001-5432-851X</orcidid></search><sort><creationdate>20240214</creationdate><title>bmp-2 Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration</title><author>Kawai, Mariko Yamamoto ; 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) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the
gene through in vivo electroporation. Natural bone tissues with skeletal muscles can be considered potential candidates for biomaterials. However, our previous system using plate-type electrodes did not achieve a 100% success rate in inducing bone tissues in skeletal muscles. In this study, we aimed to enhance the efficiency of bone tissue formation in skeletal muscles by using a non-viral
gene expression plasmid vector (pCAGGS-
) and needle-type electrodes.
We injected the
gene with pCAGGS-
into the skeletal muscles of rats' legs and immediately placed needle-type electrodes there. Skeletal tissues were then observed on the 21st day after gene transfer using soft X-ray and histological analyses.
The use of needle-type electrodes resulted in a 100% success rate in inducing bone tissues in skeletal muscles. In contrast, the plate-type electrodes only exhibited a 33% success rate. Thus, needle-type electrodes can be more efficient and reliable for transferring the
gene to skeletal muscles, making them potential biomaterials for repairing bone defects.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>38399131</pmid><doi>10.3390/ma17040880</doi><orcidid>https://orcid.org/0000-0003-3306-3238</orcidid><orcidid>https://orcid.org/0000-0001-5432-851X</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; PubMed Central Open Access |
| subjects | Biomedical materials Bones Calcification Efficiency Electric fields Electrodes Electroporation Fractures Gene expression Gene therapy Muscles Plasmids Regeneration (physiology) Soft x rays Stainless steel Vectors (Biology) |
| title | bmp-2 Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration |
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