Sequencing analysis and efficient biodiesel production by lipase from Pseudomonas aeruginosa
Background Recently, lipase processing for biodiesel production has shown a global increase as it is considered a potential alternative clean-fuel source. The current study’s objective is to investigate of lipolytic activity of lipase produced from different strains of Pseudomonas aeruginosa ( P. ae...
Gespeichert in:
| Veröffentlicht in: | Molecular biology reports 2024-12, Vol.51 (1), p.323-323, Article 323 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 323 |
|---|---|
| container_issue | 1 |
| container_start_page | 323 |
| container_title | Molecular biology reports |
| container_volume | 51 |
| creator | AL-Kadmy, Israa M. S. Aziz, Sarah Naji Hussein, Nadheema Hammood El-shafeiy, Samah N. Hamzah, Israa Hussein Suhail, Ahmed alhomaidi, Eman Algammal, Abdelazeem M. El-Saber Batiha, Gaber ElBadre, Hala M Hetta, Helal F. |
| description | Background
Recently, lipase processing for biodiesel production has shown a global increase as it is considered a potential alternative clean-fuel source. The current study’s objective is to investigate of lipolytic activity of lipase produced from different strains of
Pseudomonas aeruginosa
(
P. aeruginosa
) in biodiesel production using edible plant oils. The goal is to develop an efficient and cost-effective method for producing inexpensive and environmentally friendly biodiesel.
Methods and results
Four
P. aeruginosa
isolates were obtained from different environmental sources (soil), phenotypically identified, and it was confirmed by the PCR detection of the
16SrRNA
gene. The isolated
P. aeruginosa
strains were screened for lipase production, and the recovered lipase was purified. Besides, the lipase (
lip
) gene was detected by PCR, and the purified PCR products were sequenced and analyzed. The production of biofuel was conducted using gas chromatography among tested oils. It was found that castor oil was the best one that enhances lipase production
in-vitro
. |
| doi_str_mv | 10.1007/s11033-023-09156-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2932021994</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2931033323</sourcerecordid><originalsourceid>FETCH-LOGICAL-c326t-589afc41edbfc091de953c3755d16fa5ac7d956050b17664824ccc3edd1e59963</originalsourceid><addsrcrecordid>eNp9kE9LwzAYh4Mobk6_gAcpePFSTZqmbY4y_AcDBfUmhDR5OzLaZCbrYfv0ZutU8OAhJJDn9ya_B6Fzgq8JxuVNIARTmuIsLk5YkW4O0JiwkqY5L6tDNMYUkzSvGBmhkxAWGOOclOwYjWhFOS0qPEYfr_DZg1XGzhNpZbsOJsSDTqBpjDJgV0ltnDYQoE2W3ulerYyzSb1OWrOUAZLGuy55CdBr1zkrYxp8PzfWBXmKjhrZBjjb7xP0fn_3Nn1MZ88PT9PbWapoVqxSVnHZqJyArhsVm2jgjCpaMqZJ0UgmVak5KzDDNSmLIq-yXClFQWsCjPOCTtDVMDd-MLYJK9GZoKBtpQXXB5FxmuGMcJ5H9PIPunC9j8V31NYnzWiksoFS3oXgoRFLbzrp14JgsXUvBvciuhc792ITQxf70X3dgf6JfMuOAB2AEK_sHPzv2_-M_QIISpCO</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2931033323</pqid></control><display><type>article</type><title>Sequencing analysis and efficient biodiesel production by lipase from Pseudomonas aeruginosa</title><source>SpringerNature Journals</source><creator>AL-Kadmy, Israa M. S. ; Aziz, Sarah Naji ; Hussein, Nadheema Hammood ; El-shafeiy, Samah N. ; Hamzah, Israa Hussein ; Suhail, Ahmed ; alhomaidi, Eman ; Algammal, Abdelazeem M. ; El-Saber Batiha, Gaber ; ElBadre, Hala M ; Hetta, Helal F.</creator><creatorcontrib>AL-Kadmy, Israa M. S. ; Aziz, Sarah Naji ; Hussein, Nadheema Hammood ; El-shafeiy, Samah N. ; Hamzah, Israa Hussein ; Suhail, Ahmed ; alhomaidi, Eman ; Algammal, Abdelazeem M. ; El-Saber Batiha, Gaber ; ElBadre, Hala M ; Hetta, Helal F.</creatorcontrib><description>Background
Recently, lipase processing for biodiesel production has shown a global increase as it is considered a potential alternative clean-fuel source. The current study’s objective is to investigate of lipolytic activity of lipase produced from different strains of
Pseudomonas aeruginosa
(
P. aeruginosa
) in biodiesel production using edible plant oils. The goal is to develop an efficient and cost-effective method for producing inexpensive and environmentally friendly biodiesel.
Methods and results
Four
P. aeruginosa
isolates were obtained from different environmental sources (soil), phenotypically identified, and it was confirmed by the PCR detection of the
16SrRNA
gene. The isolated
P. aeruginosa
strains were screened for lipase production, and the recovered lipase was purified. Besides, the lipase (
lip
) gene was detected by PCR, and the purified PCR products were sequenced and analyzed. The production of biofuel was conducted using gas chromatography among tested oils. It was found that castor oil was the best one that enhances lipase production
in-vitro
.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-023-09156-z</identifier><identifier>PMID: 38393680</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Biodiesel fuels ; Biofuels ; Biomedical and Life Sciences ; Diesel ; Gas chromatography ; Histology ; Life Sciences ; Lipase ; Morphology ; Original Article ; Pseudomonas aeruginosa ; Sequence analysis</subject><ispartof>Molecular biology reports, 2024-12, Vol.51 (1), p.323-323, Article 323</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-589afc41edbfc091de953c3755d16fa5ac7d956050b17664824ccc3edd1e59963</cites><orcidid>0000-0002-5109-7338</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-023-09156-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-023-09156-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38393680$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>AL-Kadmy, Israa M. S.</creatorcontrib><creatorcontrib>Aziz, Sarah Naji</creatorcontrib><creatorcontrib>Hussein, Nadheema Hammood</creatorcontrib><creatorcontrib>El-shafeiy, Samah N.</creatorcontrib><creatorcontrib>Hamzah, Israa Hussein</creatorcontrib><creatorcontrib>Suhail, Ahmed</creatorcontrib><creatorcontrib>alhomaidi, Eman</creatorcontrib><creatorcontrib>Algammal, Abdelazeem M.</creatorcontrib><creatorcontrib>El-Saber Batiha, Gaber</creatorcontrib><creatorcontrib>ElBadre, Hala M</creatorcontrib><creatorcontrib>Hetta, Helal F.</creatorcontrib><title>Sequencing analysis and efficient biodiesel production by lipase from Pseudomonas aeruginosa</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Background
Recently, lipase processing for biodiesel production has shown a global increase as it is considered a potential alternative clean-fuel source. The current study’s objective is to investigate of lipolytic activity of lipase produced from different strains of
Pseudomonas aeruginosa
(
P. aeruginosa
) in biodiesel production using edible plant oils. The goal is to develop an efficient and cost-effective method for producing inexpensive and environmentally friendly biodiesel.
Methods and results
Four
P. aeruginosa
isolates were obtained from different environmental sources (soil), phenotypically identified, and it was confirmed by the PCR detection of the
16SrRNA
gene. The isolated
P. aeruginosa
strains were screened for lipase production, and the recovered lipase was purified. Besides, the lipase (
lip
) gene was detected by PCR, and the purified PCR products were sequenced and analyzed. The production of biofuel was conducted using gas chromatography among tested oils. It was found that castor oil was the best one that enhances lipase production
in-vitro
.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biodiesel fuels</subject><subject>Biofuels</subject><subject>Biomedical and Life Sciences</subject><subject>Diesel</subject><subject>Gas chromatography</subject><subject>Histology</subject><subject>Life Sciences</subject><subject>Lipase</subject><subject>Morphology</subject><subject>Original Article</subject><subject>Pseudomonas aeruginosa</subject><subject>Sequence analysis</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kE9LwzAYh4Mobk6_gAcpePFSTZqmbY4y_AcDBfUmhDR5OzLaZCbrYfv0ZutU8OAhJJDn9ya_B6Fzgq8JxuVNIARTmuIsLk5YkW4O0JiwkqY5L6tDNMYUkzSvGBmhkxAWGOOclOwYjWhFOS0qPEYfr_DZg1XGzhNpZbsOJsSDTqBpjDJgV0ltnDYQoE2W3ulerYyzSb1OWrOUAZLGuy55CdBr1zkrYxp8PzfWBXmKjhrZBjjb7xP0fn_3Nn1MZ88PT9PbWapoVqxSVnHZqJyArhsVm2jgjCpaMqZJ0UgmVak5KzDDNSmLIq-yXClFQWsCjPOCTtDVMDd-MLYJK9GZoKBtpQXXB5FxmuGMcJ5H9PIPunC9j8V31NYnzWiksoFS3oXgoRFLbzrp14JgsXUvBvciuhc792ITQxf70X3dgf6JfMuOAB2AEK_sHPzv2_-M_QIISpCO</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>AL-Kadmy, Israa M. S.</creator><creator>Aziz, Sarah Naji</creator><creator>Hussein, Nadheema Hammood</creator><creator>El-shafeiy, Samah N.</creator><creator>Hamzah, Israa Hussein</creator><creator>Suhail, Ahmed</creator><creator>alhomaidi, Eman</creator><creator>Algammal, Abdelazeem M.</creator><creator>El-Saber Batiha, Gaber</creator><creator>ElBadre, Hala M</creator><creator>Hetta, Helal F.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5109-7338</orcidid></search><sort><creationdate>20241201</creationdate><title>Sequencing analysis and efficient biodiesel production by lipase from Pseudomonas aeruginosa</title><author>AL-Kadmy, Israa M. S. ; Aziz, Sarah Naji ; Hussein, Nadheema Hammood ; El-shafeiy, Samah N. ; Hamzah, Israa Hussein ; Suhail, Ahmed ; alhomaidi, Eman ; Algammal, Abdelazeem M. ; El-Saber Batiha, Gaber ; ElBadre, Hala M ; Hetta, Helal F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-589afc41edbfc091de953c3755d16fa5ac7d956050b17664824ccc3edd1e59963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biodiesel fuels</topic><topic>Biofuels</topic><topic>Biomedical and Life Sciences</topic><topic>Diesel</topic><topic>Gas chromatography</topic><topic>Histology</topic><topic>Life Sciences</topic><topic>Lipase</topic><topic>Morphology</topic><topic>Original Article</topic><topic>Pseudomonas aeruginosa</topic><topic>Sequence analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>AL-Kadmy, Israa M. S.</creatorcontrib><creatorcontrib>Aziz, Sarah Naji</creatorcontrib><creatorcontrib>Hussein, Nadheema Hammood</creatorcontrib><creatorcontrib>El-shafeiy, Samah N.</creatorcontrib><creatorcontrib>Hamzah, Israa Hussein</creatorcontrib><creatorcontrib>Suhail, Ahmed</creatorcontrib><creatorcontrib>alhomaidi, Eman</creatorcontrib><creatorcontrib>Algammal, Abdelazeem M.</creatorcontrib><creatorcontrib>El-Saber Batiha, Gaber</creatorcontrib><creatorcontrib>ElBadre, Hala M</creatorcontrib><creatorcontrib>Hetta, Helal F.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>AL-Kadmy, Israa M. S.</au><au>Aziz, Sarah Naji</au><au>Hussein, Nadheema Hammood</au><au>El-shafeiy, Samah N.</au><au>Hamzah, Israa Hussein</au><au>Suhail, Ahmed</au><au>alhomaidi, Eman</au><au>Algammal, Abdelazeem M.</au><au>El-Saber Batiha, Gaber</au><au>ElBadre, Hala M</au><au>Hetta, Helal F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequencing analysis and efficient biodiesel production by lipase from Pseudomonas aeruginosa</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2024-12-01</date><risdate>2024</risdate><volume>51</volume><issue>1</issue><spage>323</spage><epage>323</epage><pages>323-323</pages><artnum>323</artnum><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Background
Recently, lipase processing for biodiesel production has shown a global increase as it is considered a potential alternative clean-fuel source. The current study’s objective is to investigate of lipolytic activity of lipase produced from different strains of
Pseudomonas aeruginosa
(
P. aeruginosa
) in biodiesel production using edible plant oils. The goal is to develop an efficient and cost-effective method for producing inexpensive and environmentally friendly biodiesel.
Methods and results
Four
P. aeruginosa
isolates were obtained from different environmental sources (soil), phenotypically identified, and it was confirmed by the PCR detection of the
16SrRNA
gene. The isolated
P. aeruginosa
strains were screened for lipase production, and the recovered lipase was purified. Besides, the lipase (
lip
) gene was detected by PCR, and the purified PCR products were sequenced and analyzed. The production of biofuel was conducted using gas chromatography among tested oils. It was found that castor oil was the best one that enhances lipase production
in-vitro
.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>38393680</pmid><doi>10.1007/s11033-023-09156-z</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-5109-7338</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-4851 |
| ispartof | Molecular biology reports, 2024-12, Vol.51 (1), p.323-323, Article 323 |
| issn | 0301-4851 1573-4978 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2932021994 |
| source | SpringerNature Journals |
| subjects | Animal Anatomy Animal Biochemistry Biodiesel fuels Biofuels Biomedical and Life Sciences Diesel Gas chromatography Histology Life Sciences Lipase Morphology Original Article Pseudomonas aeruginosa Sequence analysis |
| title | Sequencing analysis and efficient biodiesel production by lipase from Pseudomonas aeruginosa |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T23%3A59%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sequencing%20analysis%20and%20efficient%20biodiesel%20production%20by%20lipase%20from%20Pseudomonas%20aeruginosa&rft.jtitle=Molecular%20biology%20reports&rft.au=AL-Kadmy,%20Israa%20M.%20S.&rft.date=2024-12-01&rft.volume=51&rft.issue=1&rft.spage=323&rft.epage=323&rft.pages=323-323&rft.artnum=323&rft.issn=0301-4851&rft.eissn=1573-4978&rft_id=info:doi/10.1007/s11033-023-09156-z&rft_dat=%3Cproquest_cross%3E2931033323%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2931033323&rft_id=info:pmid/38393680&rfr_iscdi=true |