In vitro effects of uncarboxylated osteocalcin on buffalo Leydig cell steroidogenesis
Uncarboxylated osteocalcin (UcOCN), a bone derived circulating protein, has been demonstrated to influence steroidogenesis in testicular Leydig cells of murine and human species. However, the role of UcOCN in testosterone biosynthesis remains unexplored in domestic animals. The present study aimed t...
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| Veröffentlicht in: | Veterinary research communications 2024-06, Vol.48 (3), p.1423-1433 |
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| creator | Bharath Kumar, B. S. Mallick, Smrutirekha Manjunathachar, H. V. Shashank, C.G. Sharma, Ankur Nagoorvali, Dudekula Soren, Simson Jadhav, Vyankat Gangadhar Pandita, Sujata |
| description | Uncarboxylated osteocalcin (UcOCN), a bone derived circulating protein, has been demonstrated to influence steroidogenesis in testicular Leydig cells of murine and human species. However, the role of UcOCN in testosterone biosynthesis remains unexplored in domestic animals. The present study aimed to investigate the impact of UcOCN on the expressions of steroidogenic genes (HSD3β1, HSD3β6, CYP17A1, CYP11A1), testosterone production and GPRC6A receptor localization in buffalo Leydig cells. Leydig cells from the testes of adult Murrah buffalo were isolated, with an average cell count and viability after digestion and Percoll enrichment of 1.43 × 10
6
cells/g of testes and 78.5%, respectively. Immunophenotyping of Percoll-enriched cell suspension by flow cytometry showed populations of Leydig cells ranging between 69 and 73.9%. Immunostaining confirmed the presence of GPRC6A receptors and CYP11A1 positive Leydig cells. When these cells were cultured and incubated with varying levels of UcOCN (6, 12, 24, and 48 ng/ml) and LH, there was a significant (
P
|
| doi_str_mv | 10.1007/s11259-024-10320-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2929130580</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3063914548</sourcerecordid><originalsourceid>FETCH-LOGICAL-c326t-ac7925cda474108535759636433881f63608d2d3894a526a8c8a19bfa0e3b9fe3</originalsourceid><addsrcrecordid>eNp9kMFqGzEQhkVpaBwnL9BDEfTSyyYjjbQrHYtpG4Mhl-QstFrJrFmvUmm31G8fJXZTyCEnDeibf2Y-Qj4zuGYAzU1mjEtdARcVA-RQiQ9kwWSDVSNE_ZEsgNWyalDBObnIeQcAWgF-IueoEKSWakEe1iP9008pUh-Cd1OmMdB5dDa18e9hsJPvaMyTj84Orh9pHGk7h2CHSDf-0PVb6vww0EKk2Hdx60ef-3xJzgqS_dXpXZKHnz_uV7fV5u7XevV9Uznk9VRZ12guXWdFIxgoibKRusZaICrFQqlAdbxDpYWVvLbKKct0Gyx4bHXwuCTfjrmPKf6efZ7Mvs_PC9nRxzkbrrlm5dRy9ZJ8fYPu4pzGsp1BqFEzIYUqFD9SLsWckw_mMfV7mw6GgXmWbo7STZFuXqQbUZq-nKLndu-715Z_lguARyCXr3Hr0__Z78Q-ARQri7I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3063914548</pqid></control><display><type>article</type><title>In vitro effects of uncarboxylated osteocalcin on buffalo Leydig cell steroidogenesis</title><source>SpringerLink Journals - AutoHoldings</source><creator>Bharath Kumar, B. S. ; Mallick, Smrutirekha ; Manjunathachar, H. V. ; Shashank, C.G. ; Sharma, Ankur ; Nagoorvali, Dudekula ; Soren, Simson ; Jadhav, Vyankat Gangadhar ; Pandita, Sujata</creator><creatorcontrib>Bharath Kumar, B. S. ; Mallick, Smrutirekha ; Manjunathachar, H. V. ; Shashank, C.G. ; Sharma, Ankur ; Nagoorvali, Dudekula ; Soren, Simson ; Jadhav, Vyankat Gangadhar ; Pandita, Sujata</creatorcontrib><description>Uncarboxylated osteocalcin (UcOCN), a bone derived circulating protein, has been demonstrated to influence steroidogenesis in testicular Leydig cells of murine and human species. However, the role of UcOCN in testosterone biosynthesis remains unexplored in domestic animals. The present study aimed to investigate the impact of UcOCN on the expressions of steroidogenic genes (HSD3β1, HSD3β6, CYP17A1, CYP11A1), testosterone production and GPRC6A receptor localization in buffalo Leydig cells. Leydig cells from the testes of adult Murrah buffalo were isolated, with an average cell count and viability after digestion and Percoll enrichment of 1.43 × 10
6
cells/g of testes and 78.5%, respectively. Immunophenotyping of Percoll-enriched cell suspension by flow cytometry showed populations of Leydig cells ranging between 69 and 73.9%. Immunostaining confirmed the presence of GPRC6A receptors and CYP11A1 positive Leydig cells. When these cells were cultured and incubated with varying levels of UcOCN (6, 12, 24, and 48 ng/ml) and LH, there was a significant (
P
< 0.01) increase in testosterone production and up-regulation (
P
< 0.05) of
CYP11A1
,
CYP17A1
,
HSD3β1
and
HSD3β6
gene expression. In summary, the present study underscored the effects of UcOCN on testosterone biosynthesis, expression of crucial steroidogenic genes and interaction with GPRC6A receptors in buffalo Leydig cells, emphasizing its potential implications in andrology.</description><identifier>ISSN: 0165-7380</identifier><identifier>EISSN: 1573-7446</identifier><identifier>DOI: 10.1007/s11259-024-10320-4</identifier><identifier>PMID: 38305958</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biomedical and Life Sciences ; Biosynthesis ; Domestic animals ; Flow cytometry ; Gene expression ; Leydig cells ; Life Sciences ; Localization ; Osteocalcin ; Steroidogenesis ; Testes ; Testosterone ; Veterinary Medicine/Veterinary Science ; Zoology</subject><ispartof>Veterinary research communications, 2024-06, Vol.48 (3), p.1423-1433</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-ac7925cda474108535759636433881f63608d2d3894a526a8c8a19bfa0e3b9fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11259-024-10320-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11259-024-10320-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38305958$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bharath Kumar, B. S.</creatorcontrib><creatorcontrib>Mallick, Smrutirekha</creatorcontrib><creatorcontrib>Manjunathachar, H. V.</creatorcontrib><creatorcontrib>Shashank, C.G.</creatorcontrib><creatorcontrib>Sharma, Ankur</creatorcontrib><creatorcontrib>Nagoorvali, Dudekula</creatorcontrib><creatorcontrib>Soren, Simson</creatorcontrib><creatorcontrib>Jadhav, Vyankat Gangadhar</creatorcontrib><creatorcontrib>Pandita, Sujata</creatorcontrib><title>In vitro effects of uncarboxylated osteocalcin on buffalo Leydig cell steroidogenesis</title><title>Veterinary research communications</title><addtitle>Vet Res Commun</addtitle><addtitle>Vet Res Commun</addtitle><description>Uncarboxylated osteocalcin (UcOCN), a bone derived circulating protein, has been demonstrated to influence steroidogenesis in testicular Leydig cells of murine and human species. However, the role of UcOCN in testosterone biosynthesis remains unexplored in domestic animals. The present study aimed to investigate the impact of UcOCN on the expressions of steroidogenic genes (HSD3β1, HSD3β6, CYP17A1, CYP11A1), testosterone production and GPRC6A receptor localization in buffalo Leydig cells. Leydig cells from the testes of adult Murrah buffalo were isolated, with an average cell count and viability after digestion and Percoll enrichment of 1.43 × 10
6
cells/g of testes and 78.5%, respectively. Immunophenotyping of Percoll-enriched cell suspension by flow cytometry showed populations of Leydig cells ranging between 69 and 73.9%. Immunostaining confirmed the presence of GPRC6A receptors and CYP11A1 positive Leydig cells. When these cells were cultured and incubated with varying levels of UcOCN (6, 12, 24, and 48 ng/ml) and LH, there was a significant (
P
< 0.01) increase in testosterone production and up-regulation (
P
< 0.05) of
CYP11A1
,
CYP17A1
,
HSD3β1
and
HSD3β6
gene expression. In summary, the present study underscored the effects of UcOCN on testosterone biosynthesis, expression of crucial steroidogenic genes and interaction with GPRC6A receptors in buffalo Leydig cells, emphasizing its potential implications in andrology.</description><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Domestic animals</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Leydig cells</subject><subject>Life Sciences</subject><subject>Localization</subject><subject>Osteocalcin</subject><subject>Steroidogenesis</subject><subject>Testes</subject><subject>Testosterone</subject><subject>Veterinary Medicine/Veterinary Science</subject><subject>Zoology</subject><issn>0165-7380</issn><issn>1573-7446</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kMFqGzEQhkVpaBwnL9BDEfTSyyYjjbQrHYtpG4Mhl-QstFrJrFmvUmm31G8fJXZTyCEnDeibf2Y-Qj4zuGYAzU1mjEtdARcVA-RQiQ9kwWSDVSNE_ZEsgNWyalDBObnIeQcAWgF-IueoEKSWakEe1iP9008pUh-Cd1OmMdB5dDa18e9hsJPvaMyTj84Orh9pHGk7h2CHSDf-0PVb6vww0EKk2Hdx60ef-3xJzgqS_dXpXZKHnz_uV7fV5u7XevV9Uznk9VRZ12guXWdFIxgoibKRusZaICrFQqlAdbxDpYWVvLbKKct0Gyx4bHXwuCTfjrmPKf6efZ7Mvs_PC9nRxzkbrrlm5dRy9ZJ8fYPu4pzGsp1BqFEzIYUqFD9SLsWckw_mMfV7mw6GgXmWbo7STZFuXqQbUZq-nKLndu-715Z_lguARyCXr3Hr0__Z78Q-ARQri7I</recordid><startdate>20240601</startdate><enddate>20240601</enddate><creator>Bharath Kumar, B. S.</creator><creator>Mallick, Smrutirekha</creator><creator>Manjunathachar, H. V.</creator><creator>Shashank, C.G.</creator><creator>Sharma, Ankur</creator><creator>Nagoorvali, Dudekula</creator><creator>Soren, Simson</creator><creator>Jadhav, Vyankat Gangadhar</creator><creator>Pandita, Sujata</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20240601</creationdate><title>In vitro effects of uncarboxylated osteocalcin on buffalo Leydig cell steroidogenesis</title><author>Bharath Kumar, B. S. ; Mallick, Smrutirekha ; Manjunathachar, H. V. ; Shashank, C.G. ; Sharma, Ankur ; Nagoorvali, Dudekula ; Soren, Simson ; Jadhav, Vyankat Gangadhar ; Pandita, Sujata</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-ac7925cda474108535759636433881f63608d2d3894a526a8c8a19bfa0e3b9fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Domestic animals</topic><topic>Flow cytometry</topic><topic>Gene expression</topic><topic>Leydig cells</topic><topic>Life Sciences</topic><topic>Localization</topic><topic>Osteocalcin</topic><topic>Steroidogenesis</topic><topic>Testes</topic><topic>Testosterone</topic><topic>Veterinary Medicine/Veterinary Science</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bharath Kumar, B. S.</creatorcontrib><creatorcontrib>Mallick, Smrutirekha</creatorcontrib><creatorcontrib>Manjunathachar, H. V.</creatorcontrib><creatorcontrib>Shashank, C.G.</creatorcontrib><creatorcontrib>Sharma, Ankur</creatorcontrib><creatorcontrib>Nagoorvali, Dudekula</creatorcontrib><creatorcontrib>Soren, Simson</creatorcontrib><creatorcontrib>Jadhav, Vyankat Gangadhar</creatorcontrib><creatorcontrib>Pandita, Sujata</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bharath Kumar, B. S.</au><au>Mallick, Smrutirekha</au><au>Manjunathachar, H. V.</au><au>Shashank, C.G.</au><au>Sharma, Ankur</au><au>Nagoorvali, Dudekula</au><au>Soren, Simson</au><au>Jadhav, Vyankat Gangadhar</au><au>Pandita, Sujata</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro effects of uncarboxylated osteocalcin on buffalo Leydig cell steroidogenesis</atitle><jtitle>Veterinary research communications</jtitle><stitle>Vet Res Commun</stitle><addtitle>Vet Res Commun</addtitle><date>2024-06-01</date><risdate>2024</risdate><volume>48</volume><issue>3</issue><spage>1423</spage><epage>1433</epage><pages>1423-1433</pages><issn>0165-7380</issn><eissn>1573-7446</eissn><abstract>Uncarboxylated osteocalcin (UcOCN), a bone derived circulating protein, has been demonstrated to influence steroidogenesis in testicular Leydig cells of murine and human species. However, the role of UcOCN in testosterone biosynthesis remains unexplored in domestic animals. The present study aimed to investigate the impact of UcOCN on the expressions of steroidogenic genes (HSD3β1, HSD3β6, CYP17A1, CYP11A1), testosterone production and GPRC6A receptor localization in buffalo Leydig cells. Leydig cells from the testes of adult Murrah buffalo were isolated, with an average cell count and viability after digestion and Percoll enrichment of 1.43 × 10
6
cells/g of testes and 78.5%, respectively. Immunophenotyping of Percoll-enriched cell suspension by flow cytometry showed populations of Leydig cells ranging between 69 and 73.9%. Immunostaining confirmed the presence of GPRC6A receptors and CYP11A1 positive Leydig cells. When these cells were cultured and incubated with varying levels of UcOCN (6, 12, 24, and 48 ng/ml) and LH, there was a significant (
P
< 0.01) increase in testosterone production and up-regulation (
P
< 0.05) of
CYP11A1
,
CYP17A1
,
HSD3β1
and
HSD3β6
gene expression. In summary, the present study underscored the effects of UcOCN on testosterone biosynthesis, expression of crucial steroidogenic genes and interaction with GPRC6A receptors in buffalo Leydig cells, emphasizing its potential implications in andrology.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>38305958</pmid><doi>10.1007/s11259-024-10320-4</doi><tpages>11</tpages></addata></record> |
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| subjects | Biomedical and Life Sciences Biosynthesis Domestic animals Flow cytometry Gene expression Leydig cells Life Sciences Localization Osteocalcin Steroidogenesis Testes Testosterone Veterinary Medicine/Veterinary Science Zoology |
| title | In vitro effects of uncarboxylated osteocalcin on buffalo Leydig cell steroidogenesis |
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