LncRNA MIR100HG Promotes Cell Proliferation in Nasopharyngeal Carcinoma by Targeting miR-136-5p/IL-6 Axis
Nasopharyngeal carcinoma (NPC) features high mortality and poor prognosis. Additionally, long non-coding RNAs (lncRNAs) play a significant role in developing NPC and other types of cancer. But the functional mechanism of MIR100HG in NPC remains unclear. The long non-coding RNA MIR100HG messenger RNA...
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| Veröffentlicht in: | Molecular biotechnology 2024-05, Vol.66 (5), p.1279-1289 |
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| creator | Huang, Xiaoyan Zhong, Huaping Cai, Yunxiang |
| description | Nasopharyngeal carcinoma (NPC) features high mortality and poor prognosis. Additionally, long non-coding RNAs (lncRNAs) play a significant role in developing NPC and other types of cancer. But the functional mechanism of MIR100HG in NPC remains unclear. The long non-coding RNA MIR100HG messenger RNA (mRNA) expression was thoroughly evaluated in NPC tumors and adjacent tissues using quantitative polymerase chain reaction (qPCR). Furthermore, we employed Kaplan–Meier analysis to compare the expression of MIR100HG with survival outcomes. The CCK8 test was utilized to investigate the impact of the lncRNA MIR100HG/miR-136-5p/IL-6 axis on cell proliferation in NPC. The study’s findings indicated overexpression of the lncRNA MIR100HG in both NPC tumors and cell lines. This upregulation was associated with a poorer outcome in individuals with NPC. When lncRNA MIR100HG was knocked down in vitro, NPC cell proliferation was inhibited, resulting in tumor suppression. In certain oncogenic capacities, the lncRNA MIR100HG functions as a competitive endogenous RNA for miR-136-5p, hence impeding the inhibitory effect of miR-136-5p on its target gene, IL-6. In summary, the findings of the present investigation suggested that lncRNA MIR100HG exhibits promising characteristics as a potential indicator for the prognosis and diagnosis of NPC. |
| doi_str_mv | 10.1007/s12033-023-01028-y |
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Additionally, long non-coding RNAs (lncRNAs) play a significant role in developing NPC and other types of cancer. But the functional mechanism of MIR100HG in NPC remains unclear. The long non-coding RNA MIR100HG messenger RNA (mRNA) expression was thoroughly evaluated in NPC tumors and adjacent tissues using quantitative polymerase chain reaction (qPCR). Furthermore, we employed Kaplan–Meier analysis to compare the expression of MIR100HG with survival outcomes. The CCK8 test was utilized to investigate the impact of the lncRNA MIR100HG/miR-136-5p/IL-6 axis on cell proliferation in NPC. The study’s findings indicated overexpression of the lncRNA MIR100HG in both NPC tumors and cell lines. This upregulation was associated with a poorer outcome in individuals with NPC. When lncRNA MIR100HG was knocked down in vitro, NPC cell proliferation was inhibited, resulting in tumor suppression. In certain oncogenic capacities, the lncRNA MIR100HG functions as a competitive endogenous RNA for miR-136-5p, hence impeding the inhibitory effect of miR-136-5p on its target gene, IL-6. In summary, the findings of the present investigation suggested that lncRNA MIR100HG exhibits promising characteristics as a potential indicator for the prognosis and diagnosis of NPC.</description><identifier>ISSN: 1073-6085</identifier><identifier>EISSN: 1559-0305</identifier><identifier>DOI: 10.1007/s12033-023-01028-y</identifier><identifier>PMID: 38278928</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biochemistry ; Biological Techniques ; Biotechnology ; Cancer ; carcinoma ; Cell Biology ; Cell growth ; Cell Line, Tumor ; Cell proliferation ; Cell Proliferation - genetics ; Chemistry ; Chemistry and Materials Science ; Chromosome 5 ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic ; genes ; Human Genetics ; Humans ; Interleukin 6 ; Interleukin-6 - genetics ; Interleukin-6 - metabolism ; Male ; Medical prognosis ; messenger RNA ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Middle Aged ; mortality ; Nasopharyngeal carcinoma ; Nasopharyngeal Carcinoma - genetics ; Nasopharyngeal Carcinoma - metabolism ; Nasopharyngeal Carcinoma - pathology ; Nasopharyngeal Neoplasms - genetics ; Nasopharyngeal Neoplasms - metabolism ; Nasopharyngeal Neoplasms - pathology ; Non-coding RNA ; Original Paper ; Polymerase chain reaction ; Prognosis ; Protein Science ; quantitative polymerase chain reaction ; Ribonucleic acid ; RNA ; RNA, Long Noncoding - genetics ; RNA, Long Noncoding - metabolism ; Throat cancer ; Tumor suppression ; Tumors</subject><ispartof>Molecular biotechnology, 2024-05, Vol.66 (5), p.1279-1289</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024. 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Additionally, long non-coding RNAs (lncRNAs) play a significant role in developing NPC and other types of cancer. But the functional mechanism of MIR100HG in NPC remains unclear. The long non-coding RNA MIR100HG messenger RNA (mRNA) expression was thoroughly evaluated in NPC tumors and adjacent tissues using quantitative polymerase chain reaction (qPCR). Furthermore, we employed Kaplan–Meier analysis to compare the expression of MIR100HG with survival outcomes. The CCK8 test was utilized to investigate the impact of the lncRNA MIR100HG/miR-136-5p/IL-6 axis on cell proliferation in NPC. The study’s findings indicated overexpression of the lncRNA MIR100HG in both NPC tumors and cell lines. This upregulation was associated with a poorer outcome in individuals with NPC. When lncRNA MIR100HG was knocked down in vitro, NPC cell proliferation was inhibited, resulting in tumor suppression. In certain oncogenic capacities, the lncRNA MIR100HG functions as a competitive endogenous RNA for miR-136-5p, hence impeding the inhibitory effect of miR-136-5p on its target gene, IL-6. In summary, the findings of the present investigation suggested that lncRNA MIR100HG exhibits promising characteristics as a potential indicator for the prognosis and diagnosis of NPC.</description><subject>Biochemistry</subject><subject>Biological Techniques</subject><subject>Biotechnology</subject><subject>Cancer</subject><subject>carcinoma</subject><subject>Cell Biology</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - genetics</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromosome 5</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>genes</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Interleukin 6</subject><subject>Interleukin-6 - genetics</subject><subject>Interleukin-6 - metabolism</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>messenger RNA</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Middle Aged</subject><subject>mortality</subject><subject>Nasopharyngeal carcinoma</subject><subject>Nasopharyngeal Carcinoma - genetics</subject><subject>Nasopharyngeal Carcinoma - metabolism</subject><subject>Nasopharyngeal Carcinoma - pathology</subject><subject>Nasopharyngeal Neoplasms - genetics</subject><subject>Nasopharyngeal Neoplasms - metabolism</subject><subject>Nasopharyngeal Neoplasms - pathology</subject><subject>Non-coding RNA</subject><subject>Original Paper</subject><subject>Polymerase chain reaction</subject><subject>Prognosis</subject><subject>Protein Science</subject><subject>quantitative polymerase chain reaction</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>Throat cancer</subject><subject>Tumor suppression</subject><subject>Tumors</subject><issn>1073-6085</issn><issn>1559-0305</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2OFCEUhYnROD_6Ai4MiRs3OBcooGrZ6YwznbSj6YxrQlFUy6SqaKE6sd_e29OjJi50QYDw3UPOOYS84fCBA5irwgVIyUDg4iBqdnhGzrlSDQMJ6jmewUimoVZn5KKUBwDBVSVfkjNZC1M3oj4ncT35zd2CflptUPP2hn7JaUxzKHQZhuF4G2Ifsptjmmic6J0raffN5cO0DW6gS5d9nNLoaHug9y5vwxynLR3jhnGpmdpdrdZM08WPWF6RF70bSnj9tF-Srx-v75e3bP35ZrVcrJmXqpmZ1qYVRrkWtOJ966E2XeCtko12bWsaqHynhAYR0FhnVKca0QRfGe67vnIgL8n7k-4up-_7UGY7xuLRjJtC2hcruZK6qgUm8T9UoDRojUEh-u4v9CHt84RGLGYtpRJKHSlxonxOpeTQ212OI6ZlOdhjZ_bUmcXO7GNn9oBDb5-k9-0Yut8jv0pCQJ6Agk-Ye_7z9z9kfwLts55J</recordid><startdate>20240501</startdate><enddate>20240501</enddate><creator>Huang, Xiaoyan</creator><creator>Zhong, Huaping</creator><creator>Cai, Yunxiang</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0009-0007-8423-0215</orcidid></search><sort><creationdate>20240501</creationdate><title>LncRNA MIR100HG Promotes Cell Proliferation in Nasopharyngeal Carcinoma by Targeting miR-136-5p/IL-6 Axis</title><author>Huang, Xiaoyan ; Zhong, Huaping ; Cai, Yunxiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-667b275ab0651fbc087de1b5396abb7904cd52602e608d75d5929ec471cdf4a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biochemistry</topic><topic>Biological Techniques</topic><topic>Biotechnology</topic><topic>Cancer</topic><topic>carcinoma</topic><topic>Cell Biology</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - genetics</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromosome 5</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>genes</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Interleukin 6</topic><topic>Interleukin-6 - genetics</topic><topic>Interleukin-6 - metabolism</topic><topic>Male</topic><topic>Medical prognosis</topic><topic>messenger RNA</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Middle Aged</topic><topic>mortality</topic><topic>Nasopharyngeal carcinoma</topic><topic>Nasopharyngeal Carcinoma - genetics</topic><topic>Nasopharyngeal Carcinoma - metabolism</topic><topic>Nasopharyngeal Carcinoma - pathology</topic><topic>Nasopharyngeal Neoplasms - genetics</topic><topic>Nasopharyngeal Neoplasms - metabolism</topic><topic>Nasopharyngeal Neoplasms - pathology</topic><topic>Non-coding RNA</topic><topic>Original Paper</topic><topic>Polymerase chain reaction</topic><topic>Prognosis</topic><topic>Protein Science</topic><topic>quantitative polymerase chain reaction</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>Throat cancer</topic><topic>Tumor suppression</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Xiaoyan</creatorcontrib><creatorcontrib>Zhong, Huaping</creatorcontrib><creatorcontrib>Cai, Yunxiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Molecular biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Xiaoyan</au><au>Zhong, Huaping</au><au>Cai, Yunxiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LncRNA MIR100HG Promotes Cell Proliferation in Nasopharyngeal Carcinoma by Targeting miR-136-5p/IL-6 Axis</atitle><jtitle>Molecular biotechnology</jtitle><stitle>Mol Biotechnol</stitle><addtitle>Mol Biotechnol</addtitle><date>2024-05-01</date><risdate>2024</risdate><volume>66</volume><issue>5</issue><spage>1279</spage><epage>1289</epage><pages>1279-1289</pages><issn>1073-6085</issn><eissn>1559-0305</eissn><abstract>Nasopharyngeal carcinoma (NPC) features high mortality and poor prognosis. Additionally, long non-coding RNAs (lncRNAs) play a significant role in developing NPC and other types of cancer. But the functional mechanism of MIR100HG in NPC remains unclear. The long non-coding RNA MIR100HG messenger RNA (mRNA) expression was thoroughly evaluated in NPC tumors and adjacent tissues using quantitative polymerase chain reaction (qPCR). Furthermore, we employed Kaplan–Meier analysis to compare the expression of MIR100HG with survival outcomes. The CCK8 test was utilized to investigate the impact of the lncRNA MIR100HG/miR-136-5p/IL-6 axis on cell proliferation in NPC. The study’s findings indicated overexpression of the lncRNA MIR100HG in both NPC tumors and cell lines. This upregulation was associated with a poorer outcome in individuals with NPC. When lncRNA MIR100HG was knocked down in vitro, NPC cell proliferation was inhibited, resulting in tumor suppression. In certain oncogenic capacities, the lncRNA MIR100HG functions as a competitive endogenous RNA for miR-136-5p, hence impeding the inhibitory effect of miR-136-5p on its target gene, IL-6. In summary, the findings of the present investigation suggested that lncRNA MIR100HG exhibits promising characteristics as a potential indicator for the prognosis and diagnosis of NPC.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>38278928</pmid><doi>10.1007/s12033-023-01028-y</doi><tpages>11</tpages><orcidid>https://orcid.org/0009-0007-8423-0215</orcidid></addata></record> |
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| subjects | Biochemistry Biological Techniques Biotechnology Cancer carcinoma Cell Biology Cell growth Cell Line, Tumor Cell proliferation Cell Proliferation - genetics Chemistry Chemistry and Materials Science Chromosome 5 Female Gene expression Gene Expression Regulation, Neoplastic genes Human Genetics Humans Interleukin 6 Interleukin-6 - genetics Interleukin-6 - metabolism Male Medical prognosis messenger RNA MicroRNAs - genetics MicroRNAs - metabolism Middle Aged mortality Nasopharyngeal carcinoma Nasopharyngeal Carcinoma - genetics Nasopharyngeal Carcinoma - metabolism Nasopharyngeal Carcinoma - pathology Nasopharyngeal Neoplasms - genetics Nasopharyngeal Neoplasms - metabolism Nasopharyngeal Neoplasms - pathology Non-coding RNA Original Paper Polymerase chain reaction Prognosis Protein Science quantitative polymerase chain reaction Ribonucleic acid RNA RNA, Long Noncoding - genetics RNA, Long Noncoding - metabolism Throat cancer Tumor suppression Tumors |
| title | LncRNA MIR100HG Promotes Cell Proliferation in Nasopharyngeal Carcinoma by Targeting miR-136-5p/IL-6 Axis |
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