Dramatic fluorescence enhancement of PCN-224 and its application in “turn off” immunoassay for sensitive detection of E. coli O157:H7 in milk

•FE-PCN-224 is synthesized with the dramatic fluorescence enhancement by 20.4 times.•Ag+ ions are reduced on FE-PCN-224 to achieve high-efficiency fluorescence quenching.•The proposed immunoassay reaches a lower LOD by 29.7 times than the classical ELISA. In this study, a type of luminescent porous...

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Veröffentlicht in:	Food chemistry 2024-07, Vol.445, p.138749-138749, Article 138749
Hauptverfasser:	Fang, Bolong, Liu, Xin, Peng, Juan, Li, Yuzhi, Gong, Zhiyong, Lai, Weihua
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals E. coli O157:H7 ELISA Escherichia coli O157 Fluorescence enhancement Fluorescence quenching Food Microbiology Immunoassay - methods Metal Nanoparticles Milk - microbiology PCN-224 Silver
Online-Zugang:	Volltext
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