A novel core-shell up-conversion nanoparticles immunochromatographic assay for the detection of deoxynivalenol in cereals
Deoxynivalenol (DON) toxin is a type B group of trichothecene mycotoxins mainly originating from specific Fusarium fungi, seriously harming human and livestock health. Herein, a novel core-shell up-conversion nanoparticles immunochromatographic assay (CS-UCNPs-ICA) was developed for deoxynivalenol b...
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description | Deoxynivalenol (DON) toxin is a type B group of trichothecene mycotoxins mainly originating from specific Fusarium fungi, seriously harming human and livestock health. Herein, a novel core-shell up-conversion nanoparticles immunochromatographic assay (CS-UCNPs-ICA) was developed for deoxynivalenol based on the competitive reaction principle. By exploiting the fluorescence intensity of the T and C lines of CS-UCNPs-ICA, the concentrations of DON were obtained sensitively and precisely under optimized conditions in 5 min with a detection limit of 0.1 ng/mL. The CS-UCNPs-ICA strips only specifically detect DON and its derivatives (3-Ac-DON and 15-Ac-DON), with no cross-reaction with other mycotoxins. The low CV values illustrated a modest intra- and inter-assay variation, confirming the superior precision of this method. In the spiked experiment, the mean recoveries of corn and wheat ranged from 94.74% to 100.90% and 96.21%–104.81%, respectively. Furthermore, the approach generated results that were in good agreement with data from HPLC and ELISA analyses of naturally contaminated feed and cereals, confirming that the significant advantages of proposed strips were their high practicality, rapidness, and simplicity. Therefore, the CS-UCNPs-ICA strips platform serves as a promising candidate for developing new approaches for rapid testing or high throughput screening from DON in food products.
[Display omitted]
•Deoxynivalenol was detected by immunochromatography combined with up-conversion.•Core-shell structure effectively enhanced the up-conversion luminescence intensity.•Limit of detection of test strips for deoxynivalenol was 0.1 ng/mL.•The proposed strips had superb sensitivity, specificity, stability, and precision. |
doi_str_mv | 10.1016/j.talanta.2024.125806 |
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[Display omitted]
•Deoxynivalenol was detected by immunochromatography combined with up-conversion.•Core-shell structure effectively enhanced the up-conversion luminescence intensity.•Limit of detection of test strips for deoxynivalenol was 0.1 ng/mL.•The proposed strips had superb sensitivity, specificity, stability, and precision.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2024.125806</identifier><identifier>PMID: 38368833</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Cereals ; Deoxynivalenol ; Edible Grain - chemistry ; Enzyme-Linked Immunosorbent Assay - methods ; Food Contamination - analysis ; Fusarium - chemistry ; Humans ; Immunochromatographic assay ; Mycotoxins - analysis ; Nanoparticles ; Rapid detection ; Trichothecenes ; Up-conversion nanoparticles</subject><ispartof>Talanta (Oxford), 2024-05, Vol.272, p.125806-125806, Article 125806</ispartof><rights>2024 Elsevier B.V.</rights><rights>Copyright © 2024 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c313t-40df217b6b00f484696c6e7a61426505df45f2a8b029be0388656b36b3226e1d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.talanta.2024.125806$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38368833$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shen, Yanghong</creatorcontrib><creatorcontrib>Zhao, Xiuling</creatorcontrib><creatorcontrib>Zhang, Zaiting</creatorcontrib><creatorcontrib>Fang, Keyi</creatorcontrib><creatorcontrib>Chen, Shen</creatorcontrib><creatorcontrib>Tian, Shiyi</creatorcontrib><creatorcontrib>Fei, Jianfeng</creatorcontrib><creatorcontrib>Zhu, Junli</creatorcontrib><title>A novel core-shell up-conversion nanoparticles immunochromatographic assay for the detection of deoxynivalenol in cereals</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>Deoxynivalenol (DON) toxin is a type B group of trichothecene mycotoxins mainly originating from specific Fusarium fungi, seriously harming human and livestock health. Herein, a novel core-shell up-conversion nanoparticles immunochromatographic assay (CS-UCNPs-ICA) was developed for deoxynivalenol based on the competitive reaction principle. By exploiting the fluorescence intensity of the T and C lines of CS-UCNPs-ICA, the concentrations of DON were obtained sensitively and precisely under optimized conditions in 5 min with a detection limit of 0.1 ng/mL. The CS-UCNPs-ICA strips only specifically detect DON and its derivatives (3-Ac-DON and 15-Ac-DON), with no cross-reaction with other mycotoxins. The low CV values illustrated a modest intra- and inter-assay variation, confirming the superior precision of this method. In the spiked experiment, the mean recoveries of corn and wheat ranged from 94.74% to 100.90% and 96.21%–104.81%, respectively. Furthermore, the approach generated results that were in good agreement with data from HPLC and ELISA analyses of naturally contaminated feed and cereals, confirming that the significant advantages of proposed strips were their high practicality, rapidness, and simplicity. Therefore, the CS-UCNPs-ICA strips platform serves as a promising candidate for developing new approaches for rapid testing or high throughput screening from DON in food products.
[Display omitted]
•Deoxynivalenol was detected by immunochromatography combined with up-conversion.•Core-shell structure effectively enhanced the up-conversion luminescence intensity.•Limit of detection of test strips for deoxynivalenol was 0.1 ng/mL.•The proposed strips had superb sensitivity, specificity, stability, and precision.</description><subject>Cereals</subject><subject>Deoxynivalenol</subject><subject>Edible Grain - chemistry</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Food Contamination - analysis</subject><subject>Fusarium - chemistry</subject><subject>Humans</subject><subject>Immunochromatographic assay</subject><subject>Mycotoxins - analysis</subject><subject>Nanoparticles</subject><subject>Rapid detection</subject><subject>Trichothecenes</subject><subject>Up-conversion nanoparticles</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFr3DAQhUVJaTZpf0KDjrl4O5JsrXwKIaRtINBLexayPO5qsSVHkpfsv6-W3fQaGHgMvDfD-wj5ymDNgMlvu3U2o_HZrDnwes14o0B-ICumNqISzUZckBWAaKuW1XBJrlLaAQAXID6RS6GEVEqIFTncUx_2OFIbIlZpi-NIl7mywe8xJhc89caH2cTs7IiJumlafLDbGCaTw99o5q2z1KRkDnQIkeYt0h4z2nzMhqEs4fXg3d6M6MNInacWI5oxfSYfhyL45azX5M_3x98PP6vnXz-eHu6fKyuYyFUN_cDZppMdwFCrWrbSStwYyWouG2j6oW4GblQHvO0QhFKykZ0ow7lE1otrcnu6O8fwsmDKenLJlp7GY1iS5i1XjZKsrYu1OVltDClFHPQc3WTiQTPQR-p6p8_U9ZG6PlEvuZvzi6WbsP-fesNcDHcnA5aie4dRJ-vQW-xdLKh0H9w7L_4BwIuX4w</recordid><startdate>20240515</startdate><enddate>20240515</enddate><creator>Shen, Yanghong</creator><creator>Zhao, Xiuling</creator><creator>Zhang, Zaiting</creator><creator>Fang, Keyi</creator><creator>Chen, Shen</creator><creator>Tian, Shiyi</creator><creator>Fei, Jianfeng</creator><creator>Zhu, Junli</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20240515</creationdate><title>A novel core-shell up-conversion nanoparticles immunochromatographic assay for the detection of deoxynivalenol in cereals</title><author>Shen, Yanghong ; Zhao, Xiuling ; Zhang, Zaiting ; Fang, Keyi ; Chen, Shen ; Tian, Shiyi ; Fei, Jianfeng ; Zhu, Junli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c313t-40df217b6b00f484696c6e7a61426505df45f2a8b029be0388656b36b3226e1d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Cereals</topic><topic>Deoxynivalenol</topic><topic>Edible Grain - chemistry</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Food Contamination - analysis</topic><topic>Fusarium - chemistry</topic><topic>Humans</topic><topic>Immunochromatographic assay</topic><topic>Mycotoxins - analysis</topic><topic>Nanoparticles</topic><topic>Rapid detection</topic><topic>Trichothecenes</topic><topic>Up-conversion nanoparticles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shen, Yanghong</creatorcontrib><creatorcontrib>Zhao, Xiuling</creatorcontrib><creatorcontrib>Zhang, Zaiting</creatorcontrib><creatorcontrib>Fang, Keyi</creatorcontrib><creatorcontrib>Chen, Shen</creatorcontrib><creatorcontrib>Tian, Shiyi</creatorcontrib><creatorcontrib>Fei, Jianfeng</creatorcontrib><creatorcontrib>Zhu, Junli</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shen, Yanghong</au><au>Zhao, Xiuling</au><au>Zhang, Zaiting</au><au>Fang, Keyi</au><au>Chen, Shen</au><au>Tian, Shiyi</au><au>Fei, Jianfeng</au><au>Zhu, Junli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel core-shell up-conversion nanoparticles immunochromatographic assay for the detection of deoxynivalenol in cereals</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2024-05-15</date><risdate>2024</risdate><volume>272</volume><spage>125806</spage><epage>125806</epage><pages>125806-125806</pages><artnum>125806</artnum><issn>0039-9140</issn><eissn>1873-3573</eissn><abstract>Deoxynivalenol (DON) toxin is a type B group of trichothecene mycotoxins mainly originating from specific Fusarium fungi, seriously harming human and livestock health. Herein, a novel core-shell up-conversion nanoparticles immunochromatographic assay (CS-UCNPs-ICA) was developed for deoxynivalenol based on the competitive reaction principle. By exploiting the fluorescence intensity of the T and C lines of CS-UCNPs-ICA, the concentrations of DON were obtained sensitively and precisely under optimized conditions in 5 min with a detection limit of 0.1 ng/mL. The CS-UCNPs-ICA strips only specifically detect DON and its derivatives (3-Ac-DON and 15-Ac-DON), with no cross-reaction with other mycotoxins. The low CV values illustrated a modest intra- and inter-assay variation, confirming the superior precision of this method. In the spiked experiment, the mean recoveries of corn and wheat ranged from 94.74% to 100.90% and 96.21%–104.81%, respectively. Furthermore, the approach generated results that were in good agreement with data from HPLC and ELISA analyses of naturally contaminated feed and cereals, confirming that the significant advantages of proposed strips were their high practicality, rapidness, and simplicity. Therefore, the CS-UCNPs-ICA strips platform serves as a promising candidate for developing new approaches for rapid testing or high throughput screening from DON in food products.
[Display omitted]
•Deoxynivalenol was detected by immunochromatography combined with up-conversion.•Core-shell structure effectively enhanced the up-conversion luminescence intensity.•Limit of detection of test strips for deoxynivalenol was 0.1 ng/mL.•The proposed strips had superb sensitivity, specificity, stability, and precision.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>38368833</pmid><doi>10.1016/j.talanta.2024.125806</doi><tpages>1</tpages></addata></record> |
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subjects | Cereals Deoxynivalenol Edible Grain - chemistry Enzyme-Linked Immunosorbent Assay - methods Food Contamination - analysis Fusarium - chemistry Humans Immunochromatographic assay Mycotoxins - analysis Nanoparticles Rapid detection Trichothecenes Up-conversion nanoparticles |
title | A novel core-shell up-conversion nanoparticles immunochromatographic assay for the detection of deoxynivalenol in cereals |
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