Suberin deficiency and its effect on the transport physiology of young poplar roots
The precise functions of suberized apoplastic barriers in root water and nutrient transport physiology have not fully been elucidated. While lots of research has been performed with mutants of Arabidopsis, little to no data are available for mutants of agricultural crop or tree species. By employing...
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| Veröffentlicht in: | The New phytologist 2024-04, Vol.242 (1), p.137-153 |
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| creator | Grünhofer, Paul Heimerich, Ines Pohl, Svenja Oertel, Marlene Meng, Hongjun Zi, Lin Lucignano, Kevin Bokhari, Syed Nadeem Hussain Guo, Yayu Li, Ruili Lin, Jinxing Fladung, Matthias Kreszies, Tino Stöcker, Tyll Schoof, Heiko Schreiber, Lukas |
| description | The precise functions of suberized apoplastic barriers in root water and nutrient transport physiology have not fully been elucidated. While lots of research has been performed with mutants of Arabidopsis, little to no data are available for mutants of agricultural crop or tree species. By employing a combined set of physiological, histochemical, analytical, and transport physiological methods as well as RNA-sequencing, this study investigated the implications of remarkable CRISPR/Cas9-induced suberization defects in young roots of the economically important gray poplar. While barely affecting overall plant development, contrary to literature-based expectations significant root suberin reductions of up to 80-95% in four independent mutants were shown to not evidently affect the root hydraulic conductivity during non-stress conditions. In addition, subliminal iron deficiency symptoms and increased translocation of a photosynthesis inhibitor as well as NaCl highlight the involvement of suberin in nutrient transport physiology. The multifaceted nature of the root hydraulic conductivity does not allow drawing simplified conclusions such as that the suberin amount must always be correlated with the water transport properties of roots. However, the decreased masking of plasma membrane surface area could facilitate the uptake but also leakage of beneficial and harmful solutes. |
| doi_str_mv | 10.1111/nph.19588 |
| format | Article |
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While lots of research has been performed with mutants of Arabidopsis, little to no data are available for mutants of agricultural crop or tree species. By employing a combined set of physiological, histochemical, analytical, and transport physiological methods as well as RNA-sequencing, this study investigated the implications of remarkable CRISPR/Cas9-induced suberization defects in young roots of the economically important gray poplar. While barely affecting overall plant development, contrary to literature-based expectations significant root suberin reductions of up to 80-95% in four independent mutants were shown to not evidently affect the root hydraulic conductivity during non-stress conditions. In addition, subliminal iron deficiency symptoms and increased translocation of a photosynthesis inhibitor as well as NaCl highlight the involvement of suberin in nutrient transport physiology. The multifaceted nature of the root hydraulic conductivity does not allow drawing simplified conclusions such as that the suberin amount must always be correlated with the water transport properties of roots. 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| subjects | CRISPR Defects Economic importance Gene editing Gene sequencing Iron deficiency Mutants Nutrient deficiency Nutrient transport Photosynthesis Physiology Plant species Poplar Roots Sodium chloride Solutes Symptoms Translocation Transport properties Water transport |
| title | Suberin deficiency and its effect on the transport physiology of young poplar roots |
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