A CRISPR/Cas12 trans-cleavage reporter enabling label-free colorimetric detection of SARS-CoV-2 and its variants

We present a label-free colorimetric CRISPR/Cas-based method enabling affordable molecular diagnostics for SARS-CoV-2. This technique utilizes 3,3′-diethylthiadicarbocyanine iodide (DISC2(5)) which exhibits a distinct color transition from purple to blue when it forms dimers by inserting into the du...

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Veröffentlicht in:	Biosensors & bioelectronics 2024-05, Vol.251, p.116102-116102, Article 116102
Hauptverfasser:	Kim, Hansol, Jang, Hyowon, Song, Jayeon, Lee, Sang Mo, Lee, Seoyoung, Kwon, Hyung-Jun, Kim, Sunjoo, Kang, Taejoon, Park, Hyun Gyu
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenine Biosensing Techniques Colorimetry COVID-19 COVID-19 - diagnosis CRISPR-Cas Systems - genetics CRISPR/Cas12a Humans Molecular diagnostics Nucleic Acid Amplification Techniques RNA, Viral SARS-CoV-2 SARS-CoV-2 - genetics Variants
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