Thallium Toxicity and its Interference with Potassium Pathways Tested on Various Cell Lines
Thallium (Tl) is a highly toxic heavy metal whose mechanism of toxicity is still not completely understood. The aim of this study was to test Tl cytotoxicity on several cell lines of different tissue origin in order to clarify specific Tl toxicity to a particular organ. In addition, possible interfe...
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| Veröffentlicht in: | Biological trace element research 2024-11, Vol.202 (11), p.5025-5035 |
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| creator | Marjanović Čermak, Ana Marija Mustać, Stipe Cvjetko, Petra Pavičić, Ivan Kifer, Domagoj Bešić, Erim Domijan, Ana-Marija |
| description | Thallium (Tl) is a highly toxic heavy metal whose mechanism of toxicity is still not completely understood. The aim of this study was to test Tl cytotoxicity on several cell lines of different tissue origin in order to clarify specific Tl toxicity to a particular organ. In addition, possible interference of Tl with cell potassium (K) transport was examined. Human keratinocytes (HaCaT), human hepatocellular carcinoma (HepG2), porcine kidney epithelial cells (PK15), human neuroblastoma (SH-SY5Y) and Chinese hamster lung fibroblast cells (V79) were treated with thallium (I) acetate in a wide concentration range (3.9–500 µg/mL) for 24 h, 48 and 72 h. To assess competitive interaction between Tl and K, the cells were treated with four Tl concentrations close to IC
50
(15.63, 31.25, 62.50, 125 µg/mL) in combination with/or without potassium (I) acetate (500 µg/mL). The cells’ morphology was monitored, and cytotoxic effect was assessed by 3-(4, 5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) test. The most sensitive to Tl exposure were SH-SY5Y cells, while HepG2 were the most resistant. The combined exposure to thallium (I) acetate and potassium (I) acetate for every cell line, except V79 cells, resulted in higher cell viability compared to thallium (I) acetate alone. The results of our study indicate that cell sensitivity to Tl treatment is largely affected by tissue culture origin, its function, and Na
+
/K
+
-ATPase activity. |
| doi_str_mv | 10.1007/s12011-024-04086-8 |
| format | Article |
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50
(15.63, 31.25, 62.50, 125 µg/mL) in combination with/or without potassium (I) acetate (500 µg/mL). The cells’ morphology was monitored, and cytotoxic effect was assessed by 3-(4, 5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) test. The most sensitive to Tl exposure were SH-SY5Y cells, while HepG2 were the most resistant. The combined exposure to thallium (I) acetate and potassium (I) acetate for every cell line, except V79 cells, resulted in higher cell viability compared to thallium (I) acetate alone. The results of our study indicate that cell sensitivity to Tl treatment is largely affected by tissue culture origin, its function, and Na
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/K
+
-ATPase activity.</description><identifier>ISSN: 0163-4984</identifier><identifier>ISSN: 1559-0720</identifier><identifier>EISSN: 1559-0720</identifier><identifier>DOI: 10.1007/s12011-024-04086-8</identifier><identifier>PMID: 38349487</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Acetates ; Acetic acid ; Animals ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Cell culture ; Cell Line ; Cell lines ; Cell Survival - drug effects ; Cell viability ; Cells ; Cricetinae ; Cricetulus ; Cytotoxicity ; Dose-Response Relationship, Drug ; Epithelial cells ; Epithelium ; Heavy metals ; Hep G2 Cells ; Hepatocellular carcinoma ; Humans ; Keratinocytes ; Life Sciences ; Na+/K+-exchanging ATPase ; Neoplasms ; Nutrition ; Oncology ; Potassium ; Potassium - metabolism ; Sensitivity analysis ; Swine ; Thallium ; Thallium - toxicity ; Tissue culture ; Toxicity ; Toxicity testing</subject><ispartof>Biological trace element research, 2024-11, Vol.202 (11), p.5025-5035</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-c25e4217e304b2049e19a943e0698c8fba57bab23df4f9be3a06f02565067f673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12011-024-04086-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12011-024-04086-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38349487$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marjanović Čermak, Ana Marija</creatorcontrib><creatorcontrib>Mustać, Stipe</creatorcontrib><creatorcontrib>Cvjetko, Petra</creatorcontrib><creatorcontrib>Pavičić, Ivan</creatorcontrib><creatorcontrib>Kifer, Domagoj</creatorcontrib><creatorcontrib>Bešić, Erim</creatorcontrib><creatorcontrib>Domijan, Ana-Marija</creatorcontrib><title>Thallium Toxicity and its Interference with Potassium Pathways Tested on Various Cell Lines</title><title>Biological trace element research</title><addtitle>Biol Trace Elem Res</addtitle><addtitle>Biol Trace Elem Res</addtitle><description>Thallium (Tl) is a highly toxic heavy metal whose mechanism of toxicity is still not completely understood. The aim of this study was to test Tl cytotoxicity on several cell lines of different tissue origin in order to clarify specific Tl toxicity to a particular organ. In addition, possible interference of Tl with cell potassium (K) transport was examined. Human keratinocytes (HaCaT), human hepatocellular carcinoma (HepG2), porcine kidney epithelial cells (PK15), human neuroblastoma (SH-SY5Y) and Chinese hamster lung fibroblast cells (V79) were treated with thallium (I) acetate in a wide concentration range (3.9–500 µg/mL) for 24 h, 48 and 72 h. To assess competitive interaction between Tl and K, the cells were treated with four Tl concentrations close to IC
50
(15.63, 31.25, 62.50, 125 µg/mL) in combination with/or without potassium (I) acetate (500 µg/mL). The cells’ morphology was monitored, and cytotoxic effect was assessed by 3-(4, 5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) test. The most sensitive to Tl exposure were SH-SY5Y cells, while HepG2 were the most resistant. The combined exposure to thallium (I) acetate and potassium (I) acetate for every cell line, except V79 cells, resulted in higher cell viability compared to thallium (I) acetate alone. The results of our study indicate that cell sensitivity to Tl treatment is largely affected by tissue culture origin, its function, and Na
+
/K
+
-ATPase activity.</description><subject>Acetates</subject><subject>Acetic acid</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell culture</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cell Survival - drug effects</subject><subject>Cell viability</subject><subject>Cells</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>Cytotoxicity</subject><subject>Dose-Response Relationship, Drug</subject><subject>Epithelial cells</subject><subject>Epithelium</subject><subject>Heavy metals</subject><subject>Hep G2 Cells</subject><subject>Hepatocellular carcinoma</subject><subject>Humans</subject><subject>Keratinocytes</subject><subject>Life Sciences</subject><subject>Na+/K+-exchanging ATPase</subject><subject>Neoplasms</subject><subject>Nutrition</subject><subject>Oncology</subject><subject>Potassium</subject><subject>Potassium - metabolism</subject><subject>Sensitivity analysis</subject><subject>Swine</subject><subject>Thallium</subject><subject>Thallium - toxicity</subject><subject>Tissue culture</subject><subject>Toxicity</subject><subject>Toxicity testing</subject><issn>0163-4984</issn><issn>1559-0720</issn><issn>1559-0720</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1PGzEQhq2qqIS0f6CHylIvvSyMP9YfRxS1JVIkcghcOFjezWxjtNkN9q4g_x5DKEgcOM1hnnnn1UPIdwanDECfJcaBsQK4LECCUYX5RCasLG0BmsNnMgGmRCGtkcfkJKVbAKa5FV_IsTBCWmn0hNysNr5tw7ilq_4h1GHYU9-taRgSnXcDxgYjdjXS-zBs6LIffEpP8NIPm3u_T3SFacA17Tt67WPox0Rn2LZ0ETpMX8lR49uE317mlFz9-b2aXRSLy7_z2fmiqAVXQ1HzEiVnGgXIioO0yKy3UiAoa2rTVL7Ula-4WDeysRUKD6oBXqoSlG6UFlPy65C7i_3dmAu5bUh1ruE7zI0ct1yBLrXlGf35Dr3tx9jldk4wxoQCaWym-IGqY59SxMbtYtj6uHcM3JN6d1Dvsnr3rN6ZfPTjJXqstrh-PfnvOgPiAKS86v5hfPv9QewjzfON1Q</recordid><startdate>202411</startdate><enddate>202411</enddate><creator>Marjanović Čermak, Ana Marija</creator><creator>Mustać, Stipe</creator><creator>Cvjetko, Petra</creator><creator>Pavičić, Ivan</creator><creator>Kifer, Domagoj</creator><creator>Bešić, Erim</creator><creator>Domijan, Ana-Marija</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7QP</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>K9.</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>202411</creationdate><title>Thallium Toxicity and its Interference with Potassium Pathways Tested on Various Cell Lines</title><author>Marjanović Čermak, Ana Marija ; Mustać, Stipe ; Cvjetko, Petra ; Pavičić, Ivan ; Kifer, Domagoj ; Bešić, Erim ; Domijan, Ana-Marija</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-c25e4217e304b2049e19a943e0698c8fba57bab23df4f9be3a06f02565067f673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acetates</topic><topic>Acetic acid</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell culture</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Cell Survival - drug effects</topic><topic>Cell viability</topic><topic>Cells</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>Cytotoxicity</topic><topic>Dose-Response Relationship, Drug</topic><topic>Epithelial cells</topic><topic>Epithelium</topic><topic>Heavy metals</topic><topic>Hep G2 Cells</topic><topic>Hepatocellular carcinoma</topic><topic>Humans</topic><topic>Keratinocytes</topic><topic>Life Sciences</topic><topic>Na+/K+-exchanging ATPase</topic><topic>Neoplasms</topic><topic>Nutrition</topic><topic>Oncology</topic><topic>Potassium</topic><topic>Potassium - metabolism</topic><topic>Sensitivity analysis</topic><topic>Swine</topic><topic>Thallium</topic><topic>Thallium - toxicity</topic><topic>Tissue culture</topic><topic>Toxicity</topic><topic>Toxicity testing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marjanović Čermak, Ana Marija</creatorcontrib><creatorcontrib>Mustać, Stipe</creatorcontrib><creatorcontrib>Cvjetko, Petra</creatorcontrib><creatorcontrib>Pavičić, Ivan</creatorcontrib><creatorcontrib>Kifer, Domagoj</creatorcontrib><creatorcontrib>Bešić, Erim</creatorcontrib><creatorcontrib>Domijan, Ana-Marija</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Biological trace element research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marjanović Čermak, Ana Marija</au><au>Mustać, Stipe</au><au>Cvjetko, Petra</au><au>Pavičić, Ivan</au><au>Kifer, Domagoj</au><au>Bešić, Erim</au><au>Domijan, Ana-Marija</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Thallium Toxicity and its Interference with Potassium Pathways Tested on Various Cell Lines</atitle><jtitle>Biological trace element research</jtitle><stitle>Biol Trace Elem Res</stitle><addtitle>Biol Trace Elem Res</addtitle><date>2024-11</date><risdate>2024</risdate><volume>202</volume><issue>11</issue><spage>5025</spage><epage>5035</epage><pages>5025-5035</pages><issn>0163-4984</issn><issn>1559-0720</issn><eissn>1559-0720</eissn><abstract>Thallium (Tl) is a highly toxic heavy metal whose mechanism of toxicity is still not completely understood. The aim of this study was to test Tl cytotoxicity on several cell lines of different tissue origin in order to clarify specific Tl toxicity to a particular organ. In addition, possible interference of Tl with cell potassium (K) transport was examined. Human keratinocytes (HaCaT), human hepatocellular carcinoma (HepG2), porcine kidney epithelial cells (PK15), human neuroblastoma (SH-SY5Y) and Chinese hamster lung fibroblast cells (V79) were treated with thallium (I) acetate in a wide concentration range (3.9–500 µg/mL) for 24 h, 48 and 72 h. To assess competitive interaction between Tl and K, the cells were treated with four Tl concentrations close to IC
50
(15.63, 31.25, 62.50, 125 µg/mL) in combination with/or without potassium (I) acetate (500 µg/mL). The cells’ morphology was monitored, and cytotoxic effect was assessed by 3-(4, 5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) test. The most sensitive to Tl exposure were SH-SY5Y cells, while HepG2 were the most resistant. The combined exposure to thallium (I) acetate and potassium (I) acetate for every cell line, except V79 cells, resulted in higher cell viability compared to thallium (I) acetate alone. The results of our study indicate that cell sensitivity to Tl treatment is largely affected by tissue culture origin, its function, and Na
+
/K
+
-ATPase activity.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>38349487</pmid><doi>10.1007/s12011-024-04086-8</doi><tpages>11</tpages></addata></record> |
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| subjects | Acetates Acetic acid Animals Biochemistry Biomedical and Life Sciences Biotechnology Cell culture Cell Line Cell lines Cell Survival - drug effects Cell viability Cells Cricetinae Cricetulus Cytotoxicity Dose-Response Relationship, Drug Epithelial cells Epithelium Heavy metals Hep G2 Cells Hepatocellular carcinoma Humans Keratinocytes Life Sciences Na+/K+-exchanging ATPase Neoplasms Nutrition Oncology Potassium Potassium - metabolism Sensitivity analysis Swine Thallium Thallium - toxicity Tissue culture Toxicity Toxicity testing |
| title | Thallium Toxicity and its Interference with Potassium Pathways Tested on Various Cell Lines |
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