A cell line derived from heart of rainbow trout is refractory to Tilapia lake virus
Cell lines are important in vitro models to answer biological mechanisms with less genetic variations. The present study was attempted to develop a cell line from rainbow trout, where we obtained a cell line from the heart, named “RBT‐H.” The cell line was authenticated using karyotyping and cytochr...
Gespeichert in:
| Veröffentlicht in: | Cell biology international 2024-03, Vol.48 (3), p.347-357 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 357 |
|---|---|
| container_issue | 3 |
| container_start_page | 347 |
| container_title | Cell biology international |
| container_volume | 48 |
| creator | Pande, Amit Thakuria, Dimpal Kushwaha, Basdeo Kumar, Ravindra S, Murali Rastogi, Aakriti Sood, Neeraj |
| description | Cell lines are important in vitro models to answer biological mechanisms with less genetic variations. The present study was attempted to develop a cell line from rainbow trout, where we obtained a cell line from the heart, named “RBT‐H.” The cell line was authenticated using karyotyping and cytochrome c oxidase subunit I (COI) gene sequencing. The karyotype demonstrated diploid chromosome number (2n) as 62 and the sequence of partial COI gene was 99.84% similar to rainbow trout COI data set, both suggesting the origin of RBT‐H from the rainbow trout. The heart cell line was mycoplasma‐free and found to be refractory to infection with the Tilapia lake virus. The RBT‐H cell line is deposited in the National Repository of Fish Cell Line (NRFC) at ICAR‐NBFGR, Lucknow, India, with Accession no. NRFC0075 for maintenance and distribution to researchers on request for R&D. |
| doi_str_mv | 10.1002/cbin.12125 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2926074699</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2926074699</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3165-cd9c51827305b848174a66f16550f242a7dc681f6d135872883b92b68620ba103</originalsourceid><addsrcrecordid>eNp90E1OwzAQBWALgWgpbDgAssQGIaV4nNhxlqXip1IFm7KOnMQRLmlc7KRVb8NZOBkOKSxYsPJI_vQ08xA6BzIGQuhNnul6DBQoO0BDIAkLRMjYYTdzFvAkYQN04tySEIBI8GM0CIXXSQRDtJjgXFUVrnStcKGs3qgCl9as8KuStsGmxFbqOjNb3FjTNlg7bFVpZd4Yu8ONwQtdybWWnx-VfFN4o23rTtFRKSunzvbvCL3c3y2mj8H8-WE2ncyDPAS_WV4kOQNB45CwTEQC4khyXvovRkoaURkXORdQ8gJCJmIqRJglNOOCU5JJIOEIXfW5a2veW-WadKVdd46slWldShPKSRz5Bjy9_EOXprW1365TLBQACfPqule5Nc75O9O11StpdymQtOs67bpOv7v2-GIf2WYrVfzSn3I9gB5sdaV2_0Sl09vZUx_6BT1ehz0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2925381195</pqid></control><display><type>article</type><title>A cell line derived from heart of rainbow trout is refractory to Tilapia lake virus</title><source>Access via Wiley Online Library</source><creator>Pande, Amit ; Thakuria, Dimpal ; Kushwaha, Basdeo ; Kumar, Ravindra ; S, Murali ; Rastogi, Aakriti ; Sood, Neeraj</creator><creatorcontrib>Pande, Amit ; Thakuria, Dimpal ; Kushwaha, Basdeo ; Kumar, Ravindra ; S, Murali ; Rastogi, Aakriti ; Sood, Neeraj</creatorcontrib><description>Cell lines are important in vitro models to answer biological mechanisms with less genetic variations. The present study was attempted to develop a cell line from rainbow trout, where we obtained a cell line from the heart, named “RBT‐H.” The cell line was authenticated using karyotyping and cytochrome c oxidase subunit I (COI) gene sequencing. The karyotype demonstrated diploid chromosome number (2n) as 62 and the sequence of partial COI gene was 99.84% similar to rainbow trout COI data set, both suggesting the origin of RBT‐H from the rainbow trout. The heart cell line was mycoplasma‐free and found to be refractory to infection with the Tilapia lake virus. The RBT‐H cell line is deposited in the National Repository of Fish Cell Line (NRFC) at ICAR‐NBFGR, Lucknow, India, with Accession no. NRFC0075 for maintenance and distribution to researchers on request for R&D.</description><identifier>ISSN: 1065-6995</identifier><identifier>EISSN: 1095-8355</identifier><identifier>DOI: 10.1002/cbin.12125</identifier><identifier>PMID: 38212941</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>cell line ; Cell lines ; Chromosome number ; COI protein ; Cytochrome-c oxidase ; Diploids ; Genetic diversity ; Heart ; Karyotypes ; karyotyping ; Oncorhynchus mykiss ; rainbow trout ; TiLV ; transfection</subject><ispartof>Cell biology international, 2024-03, Vol.48 (3), p.347-357</ispartof><rights>2024 International Federation of Cell Biology.</rights><rights>2024 International Federation for Cell Biology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3165-cd9c51827305b848174a66f16550f242a7dc681f6d135872883b92b68620ba103</cites><orcidid>0000-0001-6044-5835 ; 0000-0002-2046-7957 ; 0000-0001-6794-2471</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbin.12125$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbin.12125$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27931,27932,45581,45582</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38212941$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pande, Amit</creatorcontrib><creatorcontrib>Thakuria, Dimpal</creatorcontrib><creatorcontrib>Kushwaha, Basdeo</creatorcontrib><creatorcontrib>Kumar, Ravindra</creatorcontrib><creatorcontrib>S, Murali</creatorcontrib><creatorcontrib>Rastogi, Aakriti</creatorcontrib><creatorcontrib>Sood, Neeraj</creatorcontrib><title>A cell line derived from heart of rainbow trout is refractory to Tilapia lake virus</title><title>Cell biology international</title><addtitle>Cell Biol Int</addtitle><description>Cell lines are important in vitro models to answer biological mechanisms with less genetic variations. The present study was attempted to develop a cell line from rainbow trout, where we obtained a cell line from the heart, named “RBT‐H.” The cell line was authenticated using karyotyping and cytochrome c oxidase subunit I (COI) gene sequencing. The karyotype demonstrated diploid chromosome number (2n) as 62 and the sequence of partial COI gene was 99.84% similar to rainbow trout COI data set, both suggesting the origin of RBT‐H from the rainbow trout. The heart cell line was mycoplasma‐free and found to be refractory to infection with the Tilapia lake virus. The RBT‐H cell line is deposited in the National Repository of Fish Cell Line (NRFC) at ICAR‐NBFGR, Lucknow, India, with Accession no. NRFC0075 for maintenance and distribution to researchers on request for R&D.</description><subject>cell line</subject><subject>Cell lines</subject><subject>Chromosome number</subject><subject>COI protein</subject><subject>Cytochrome-c oxidase</subject><subject>Diploids</subject><subject>Genetic diversity</subject><subject>Heart</subject><subject>Karyotypes</subject><subject>karyotyping</subject><subject>Oncorhynchus mykiss</subject><subject>rainbow trout</subject><subject>TiLV</subject><subject>transfection</subject><issn>1065-6995</issn><issn>1095-8355</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp90E1OwzAQBWALgWgpbDgAssQGIaV4nNhxlqXip1IFm7KOnMQRLmlc7KRVb8NZOBkOKSxYsPJI_vQ08xA6BzIGQuhNnul6DBQoO0BDIAkLRMjYYTdzFvAkYQN04tySEIBI8GM0CIXXSQRDtJjgXFUVrnStcKGs3qgCl9as8KuStsGmxFbqOjNb3FjTNlg7bFVpZd4Yu8ONwQtdybWWnx-VfFN4o23rTtFRKSunzvbvCL3c3y2mj8H8-WE2ncyDPAS_WV4kOQNB45CwTEQC4khyXvovRkoaURkXORdQ8gJCJmIqRJglNOOCU5JJIOEIXfW5a2veW-WadKVdd46slWldShPKSRz5Bjy9_EOXprW1365TLBQACfPqule5Nc75O9O11StpdymQtOs67bpOv7v2-GIf2WYrVfzSn3I9gB5sdaV2_0Sl09vZUx_6BT1ehz0</recordid><startdate>202403</startdate><enddate>202403</enddate><creator>Pande, Amit</creator><creator>Thakuria, Dimpal</creator><creator>Kushwaha, Basdeo</creator><creator>Kumar, Ravindra</creator><creator>S, Murali</creator><creator>Rastogi, Aakriti</creator><creator>Sood, Neeraj</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6044-5835</orcidid><orcidid>https://orcid.org/0000-0002-2046-7957</orcidid><orcidid>https://orcid.org/0000-0001-6794-2471</orcidid></search><sort><creationdate>202403</creationdate><title>A cell line derived from heart of rainbow trout is refractory to Tilapia lake virus</title><author>Pande, Amit ; Thakuria, Dimpal ; Kushwaha, Basdeo ; Kumar, Ravindra ; S, Murali ; Rastogi, Aakriti ; Sood, Neeraj</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3165-cd9c51827305b848174a66f16550f242a7dc681f6d135872883b92b68620ba103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>cell line</topic><topic>Cell lines</topic><topic>Chromosome number</topic><topic>COI protein</topic><topic>Cytochrome-c oxidase</topic><topic>Diploids</topic><topic>Genetic diversity</topic><topic>Heart</topic><topic>Karyotypes</topic><topic>karyotyping</topic><topic>Oncorhynchus mykiss</topic><topic>rainbow trout</topic><topic>TiLV</topic><topic>transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pande, Amit</creatorcontrib><creatorcontrib>Thakuria, Dimpal</creatorcontrib><creatorcontrib>Kushwaha, Basdeo</creatorcontrib><creatorcontrib>Kumar, Ravindra</creatorcontrib><creatorcontrib>S, Murali</creatorcontrib><creatorcontrib>Rastogi, Aakriti</creatorcontrib><creatorcontrib>Sood, Neeraj</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pande, Amit</au><au>Thakuria, Dimpal</au><au>Kushwaha, Basdeo</au><au>Kumar, Ravindra</au><au>S, Murali</au><au>Rastogi, Aakriti</au><au>Sood, Neeraj</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A cell line derived from heart of rainbow trout is refractory to Tilapia lake virus</atitle><jtitle>Cell biology international</jtitle><addtitle>Cell Biol Int</addtitle><date>2024-03</date><risdate>2024</risdate><volume>48</volume><issue>3</issue><spage>347</spage><epage>357</epage><pages>347-357</pages><issn>1065-6995</issn><eissn>1095-8355</eissn><abstract>Cell lines are important in vitro models to answer biological mechanisms with less genetic variations. The present study was attempted to develop a cell line from rainbow trout, where we obtained a cell line from the heart, named “RBT‐H.” The cell line was authenticated using karyotyping and cytochrome c oxidase subunit I (COI) gene sequencing. The karyotype demonstrated diploid chromosome number (2n) as 62 and the sequence of partial COI gene was 99.84% similar to rainbow trout COI data set, both suggesting the origin of RBT‐H from the rainbow trout. The heart cell line was mycoplasma‐free and found to be refractory to infection with the Tilapia lake virus. The RBT‐H cell line is deposited in the National Repository of Fish Cell Line (NRFC) at ICAR‐NBFGR, Lucknow, India, with Accession no. NRFC0075 for maintenance and distribution to researchers on request for R&D.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>38212941</pmid><doi>10.1002/cbin.12125</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-6044-5835</orcidid><orcidid>https://orcid.org/0000-0002-2046-7957</orcidid><orcidid>https://orcid.org/0000-0001-6794-2471</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1065-6995 |
| ispartof | Cell biology international, 2024-03, Vol.48 (3), p.347-357 |
| issn | 1065-6995 1095-8355 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2926074699 |
| source | Access via Wiley Online Library |
| subjects | cell line Cell lines Chromosome number COI protein Cytochrome-c oxidase Diploids Genetic diversity Heart Karyotypes karyotyping Oncorhynchus mykiss rainbow trout TiLV transfection |
| title | A cell line derived from heart of rainbow trout is refractory to Tilapia lake virus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-04T06%3A59%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20cell%20line%20derived%20from%20heart%20of%20rainbow%20trout%20is%20refractory%20to%20Tilapia%C2%A0lake%20virus&rft.jtitle=Cell%20biology%20international&rft.au=Pande,%20Amit&rft.date=2024-03&rft.volume=48&rft.issue=3&rft.spage=347&rft.epage=357&rft.pages=347-357&rft.issn=1065-6995&rft.eissn=1095-8355&rft_id=info:doi/10.1002/cbin.12125&rft_dat=%3Cproquest_cross%3E2926074699%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2925381195&rft_id=info:pmid/38212941&rfr_iscdi=true |