Near-Infrared Fluorescent Silica Nanoparticles Based on Gold–Silver Alloy Nanoclusters for Clinical Diagnosis
In clinical diagnosis, fluorescent particles are applied to detect analytes in biofluids, such as blood and saliva. However, current fluorescence detection methods have not been optimized to account for the overlapping autofluorescence peaks of biological substances. Gold and silver nanoclusters are...
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| Veröffentlicht in: | Chemical & pharmaceutical bulletin 2024/01/30, Vol.72(1), pp.121-126 |
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| creator | Ichimaru, Hiroaki Kikuchi, Shigetoshi |
| description | In clinical diagnosis, fluorescent particles are applied to detect analytes in biofluids, such as blood and saliva. However, current fluorescence detection methods have not been optimized to account for the overlapping autofluorescence peaks of biological substances. Gold and silver nanoclusters are known to the novel fluorescent materials and their emission wavelengths depend on cluster size. In this study, we developed fluorescent silica nanoparticles using gold–silver alloy nanoclusters and chitosan (CS) (NH2-SiO2@Au@CS@AuAg) by the layer-by-layer method. Under UV-light irradiation at 365 nm, the emission wavelength of NH2-SiO2@Au@CS@AuAg reached 750 nm in the near-IR region. Scanning electron microscopy images revealed that the shape of NH2-SiO2@Au@CS@AuAg was uniform and spherical. The fluorescence spectrum of horse blood obtained in the presence of NH2-SiO2@Au@CS@AuAg contained a specific fluorescence peak attributed to NH2-SiO2@Au@CS@AuAg, which was distinguishable from the autofluorescence peaks. These results showed that NH2-SiO2@Au@CS@AuAg has advantageous fluorescence properties for clinical diagnostic applications. |
| doi_str_mv | 10.1248/cpb.c23-00688 |
| format | Article |
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However, current fluorescence detection methods have not been optimized to account for the overlapping autofluorescence peaks of biological substances. Gold and silver nanoclusters are known to the novel fluorescent materials and their emission wavelengths depend on cluster size. In this study, we developed fluorescent silica nanoparticles using gold–silver alloy nanoclusters and chitosan (CS) (NH2-SiO2@Au@CS@AuAg) by the layer-by-layer method. Under UV-light irradiation at 365 nm, the emission wavelength of NH2-SiO2@Au@CS@AuAg reached 750 nm in the near-IR region. Scanning electron microscopy images revealed that the shape of NH2-SiO2@Au@CS@AuAg was uniform and spherical. The fluorescence spectrum of horse blood obtained in the presence of NH2-SiO2@Au@CS@AuAg contained a specific fluorescence peak attributed to NH2-SiO2@Au@CS@AuAg, which was distinguishable from the autofluorescence peaks. These results showed that NH2-SiO2@Au@CS@AuAg has advantageous fluorescence properties for clinical diagnostic applications.</description><identifier>ISSN: 0009-2363</identifier><identifier>EISSN: 1347-5223</identifier><identifier>DOI: 10.1248/cpb.c23-00688</identifier><identifier>PMID: 38296514</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>Blood ; Chitosan ; clinical diagnosis ; Diagnosis ; Emission analysis ; Fluorescence ; fluorescent silica nanoparticle ; Gold ; Gold base alloys ; gold–silver alloy nanocluster ; layer-by-layer method ; Light irradiation ; Nanoalloys ; Nanoclusters ; Nanoparticles ; Near infrared radiation ; near-IR emission ; Saliva ; Scanning electron microscopy ; Silicon dioxide ; Silver ; Ultraviolet radiation</subject><ispartof>Chemical and Pharmaceutical Bulletin, 2024/01/30, Vol.72(1), pp.121-126</ispartof><rights>2024 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c499t-bceafc77e699ee5d6a5a89f90d75e720f7d537dc9cd423aa1d5aafb675e70e983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38296514$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ichimaru, Hiroaki</creatorcontrib><creatorcontrib>Kikuchi, Shigetoshi</creatorcontrib><title>Near-Infrared Fluorescent Silica Nanoparticles Based on Gold–Silver Alloy Nanoclusters for Clinical Diagnosis</title><title>Chemical & pharmaceutical bulletin</title><addtitle>Chem. Pharm. Bull.</addtitle><description>In clinical diagnosis, fluorescent particles are applied to detect analytes in biofluids, such as blood and saliva. However, current fluorescence detection methods have not been optimized to account for the overlapping autofluorescence peaks of biological substances. Gold and silver nanoclusters are known to the novel fluorescent materials and their emission wavelengths depend on cluster size. In this study, we developed fluorescent silica nanoparticles using gold–silver alloy nanoclusters and chitosan (CS) (NH2-SiO2@Au@CS@AuAg) by the layer-by-layer method. Under UV-light irradiation at 365 nm, the emission wavelength of NH2-SiO2@Au@CS@AuAg reached 750 nm in the near-IR region. Scanning electron microscopy images revealed that the shape of NH2-SiO2@Au@CS@AuAg was uniform and spherical. The fluorescence spectrum of horse blood obtained in the presence of NH2-SiO2@Au@CS@AuAg contained a specific fluorescence peak attributed to NH2-SiO2@Au@CS@AuAg, which was distinguishable from the autofluorescence peaks. These results showed that NH2-SiO2@Au@CS@AuAg has advantageous fluorescence properties for clinical diagnostic applications.</description><subject>Blood</subject><subject>Chitosan</subject><subject>clinical diagnosis</subject><subject>Diagnosis</subject><subject>Emission analysis</subject><subject>Fluorescence</subject><subject>fluorescent silica nanoparticle</subject><subject>Gold</subject><subject>Gold base alloys</subject><subject>gold–silver alloy nanocluster</subject><subject>layer-by-layer method</subject><subject>Light irradiation</subject><subject>Nanoalloys</subject><subject>Nanoclusters</subject><subject>Nanoparticles</subject><subject>Near infrared radiation</subject><subject>near-IR emission</subject><subject>Saliva</subject><subject>Scanning electron microscopy</subject><subject>Silicon dioxide</subject><subject>Silver</subject><subject>Ultraviolet radiation</subject><issn>0009-2363</issn><issn>1347-5223</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNpd0btuFDEUBmALgcgSKGmRJRqaCb6Mx-MyLCREikIB1NZZ-0yYldde7BmkdLwDb8iT4L2wSDR2cT79ss9PyEvOLrho-7duu7pwQjaMdX3_iCy4bHWjhJCPyYIxZhohO3lGnpWyZkwopuVTciZ7YTrF2wVJdwi5uYlDhoyeXoU5ZSwO40Q_j2F0QO8gpi3kaXQBC30HpbIU6XUK_vfPXxX9wEwvQ0gPe-rCXCbMhQ4p02UYY80I9P0I9zGVsTwnTwYIBV8c73Py9erDl-XH5vbT9c3y8rZxrTFTs3IIg9MaO2MQle9AQW8Gw7xWqAUbtFdSe2ecb4UE4F4BDKtuN2VoenlO3hxytzl9n7FMdjPWb4UAEdNcrDBCGCU4V5W-_o-u05xjfd1OdVIJpXRVzUG5nErJONhtHjeQHyxndteErU3Y2oTdN1H9q2PqvNqgP-m_q69geQDrMsE9nsBx1fs4LSzfHafYf9NvkC1G-Qd0tZ7D</recordid><startdate>20240101</startdate><enddate>20240101</enddate><creator>Ichimaru, Hiroaki</creator><creator>Kikuchi, Shigetoshi</creator><general>The Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20240101</creationdate><title>Near-Infrared Fluorescent Silica Nanoparticles Based on Gold–Silver Alloy Nanoclusters for Clinical Diagnosis</title><author>Ichimaru, Hiroaki ; Kikuchi, Shigetoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-bceafc77e699ee5d6a5a89f90d75e720f7d537dc9cd423aa1d5aafb675e70e983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Blood</topic><topic>Chitosan</topic><topic>clinical diagnosis</topic><topic>Diagnosis</topic><topic>Emission analysis</topic><topic>Fluorescence</topic><topic>fluorescent silica nanoparticle</topic><topic>Gold</topic><topic>Gold base alloys</topic><topic>gold–silver alloy nanocluster</topic><topic>layer-by-layer method</topic><topic>Light irradiation</topic><topic>Nanoalloys</topic><topic>Nanoclusters</topic><topic>Nanoparticles</topic><topic>Near infrared radiation</topic><topic>near-IR emission</topic><topic>Saliva</topic><topic>Scanning electron microscopy</topic><topic>Silicon dioxide</topic><topic>Silver</topic><topic>Ultraviolet radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ichimaru, Hiroaki</creatorcontrib><creatorcontrib>Kikuchi, Shigetoshi</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chemical & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ichimaru, Hiroaki</au><au>Kikuchi, Shigetoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Near-Infrared Fluorescent Silica Nanoparticles Based on Gold–Silver Alloy Nanoclusters for Clinical Diagnosis</atitle><jtitle>Chemical & pharmaceutical bulletin</jtitle><addtitle>Chem. Pharm. Bull.</addtitle><date>2024-01-01</date><risdate>2024</risdate><volume>72</volume><issue>1</issue><spage>121</spage><epage>126</epage><pages>121-126</pages><artnum>c23-00688</artnum><issn>0009-2363</issn><eissn>1347-5223</eissn><abstract>In clinical diagnosis, fluorescent particles are applied to detect analytes in biofluids, such as blood and saliva. However, current fluorescence detection methods have not been optimized to account for the overlapping autofluorescence peaks of biological substances. Gold and silver nanoclusters are known to the novel fluorescent materials and their emission wavelengths depend on cluster size. In this study, we developed fluorescent silica nanoparticles using gold–silver alloy nanoclusters and chitosan (CS) (NH2-SiO2@Au@CS@AuAg) by the layer-by-layer method. Under UV-light irradiation at 365 nm, the emission wavelength of NH2-SiO2@Au@CS@AuAg reached 750 nm in the near-IR region. Scanning electron microscopy images revealed that the shape of NH2-SiO2@Au@CS@AuAg was uniform and spherical. The fluorescence spectrum of horse blood obtained in the presence of NH2-SiO2@Au@CS@AuAg contained a specific fluorescence peak attributed to NH2-SiO2@Au@CS@AuAg, which was distinguishable from the autofluorescence peaks. These results showed that NH2-SiO2@Au@CS@AuAg has advantageous fluorescence properties for clinical diagnostic applications.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>38296514</pmid><doi>10.1248/cpb.c23-00688</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Free Full-Text Journals in Chemistry |
| subjects | Blood Chitosan clinical diagnosis Diagnosis Emission analysis Fluorescence fluorescent silica nanoparticle Gold Gold base alloys gold–silver alloy nanocluster layer-by-layer method Light irradiation Nanoalloys Nanoclusters Nanoparticles Near infrared radiation near-IR emission Saliva Scanning electron microscopy Silicon dioxide Silver Ultraviolet radiation |
| title | Near-Infrared Fluorescent Silica Nanoparticles Based on Gold–Silver Alloy Nanoclusters for Clinical Diagnosis |
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