The use of proteases to remove protein-based residues from solid surfaces

The use of proteases to remove protein-based residues from solid surfaces

The efficiency of the commercial proteolytic preparation ‘Alcalase’ in removing model proteins (casein, BSA and haemoglobin) experimentally burned onto a model solid surface (glass) was investigated. The cleaning efficiency was monitored qualitatively by direct observation and quantitatively by moni...

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Veröffentlicht in:Process biochemistry (1991) 2005-10, Vol.40 (10), p.3377-3382
Hauptverfasser: Turner, Kevin, Serantoni, Marina, Boyce, Angela, Walsh, Gary
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Sprache:eng
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Alcalase
Enzyme-cleaning
Fouling
Glass
Protease
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container_issue 10
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container_title Process biochemistry (1991)
container_volume 40
creator Turner, Kevin
Serantoni, Marina
Boyce, Angela
Walsh, Gary
description The efficiency of the commercial proteolytic preparation ‘Alcalase’ in removing model proteins (casein, BSA and haemoglobin) experimentally burned onto a model solid surface (glass) was investigated. The cleaning efficiency was monitored qualitatively by direct observation and quantitatively by monitoring polypeptide/peptide/amino acid accumulation in the wash and by three-dimensional atomic force microscopy. The results indicate that treatment with enzyme at industrially realistic inclusion levels removes a very significant proportion of protein foulant and that post-cleaning residual enzyme activity on the glass surface can be easily removed or inactivated. Results are discussed in the context of their potential application in the bioprocess sector.
doi_str_mv 10.1016/j.procbio.2005.03.040
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1873-3298
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subjects Alcalase
Enzyme-cleaning
Fouling
Glass
Protease
title The use of proteases to remove protein-based residues from solid surfaces
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